In Brief:
IMPLICATIONS: The low aqueous solubility of nonpolar groups (hydrophobicity) is a powerful thermodynamic force determining the conformation, and thus the function, of macromolecules such as proteins. The inhaled anesthetic isoflurane does not affect the aqueous solubility of two simple nonpolar molecules (methane and toluene) in concentrations used for clinical anesthesia. These results argue against alteration in the aqueous solubility of nonpolar groups on macromolecules as a primary component of the mechanism of action of inhaled anesthetics.
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