Secondary Logo

Journal Logo

Anesthetics Discriminate Between Tonic and Phasic γ-Aminobutyric Acid Receptors on Hippocampal CA1 Neurons

Bieda, Mark C. PhD; Su, Henry BS; MacIver, M Bruce MSc, PhD

doi: 10.1213/ane.0b013e3181904571
Anesthetic Pharmacology: Research Reports
Free
SDC

BACKGROUND: Anesthesia is produced by a depression of neuronal signaling in the central nervous system (CNS); however, the mechanism(s) of action underlying this depression remain unclear. Recent studies have indicated that anesthetics can enhance inhibition of CNS neurons by increasing current flow through tonic γ-aminobutyric acid (GABAA) receptor gated chloride channels in their membranes. Enhanced tonic inhibition would contribute to CNS depression produced by anesthetics, but it remains to be determined to what extent anesthetic actions at these receptors contribute to CNS depression. In the present study, we compared and contrasted the involvement of tonic versus synaptic GABAA receptors in the functional depression of CNS neurons produced by isoflurane and thiopental.

METHODS: In rat hippocampal slices, whole cell patch clamp recordings were used to study anesthetic effects on CA1 neuron intrinsic excitability, and population spike recordings were used to investigate effects on synaptically evoked discharge. These responses were chosen to test whether anesthetic effects on GABA receptors alter single neuron discharge and/or circuit level synaptic functioning. Phasic (synaptic) GABA receptors were selectively blocked using the GABAA antagonist gabazine and tonic responses were blocked using the chloride channel blocker picrotoxin.

RESULTS: Clinically relevant and equi-effective concentrations of thiopental and isoflurane depressed CA1 neuron synaptically evoked discharge. This depression was partially reversed by blocking synaptic GABAA receptors with gabazine (20 μM). The thiopental-induced depression was reversed by approximately 60%, but the isoflurane-induced depression was reversed by only approximately 20%. Blocking tonic GABAA receptors with the addition of 100 μM picrotoxin produced an additional 40% reversal of the thiopental-induced depression, but no additional reversal was seen for isoflurane-depressed responses. In response to direct DC current injection, CA1 neuron discharge was depressed by thiopental and membrane conductance was increased. Both of these effects were reversed by picrotoxin, but not by gabazine. Isoflurane, in contrast, neither depressed current-evoked discharge, nor altered the membrane conductance of CA1 neurons.

CONCLUSIONS: These results indicate that general anesthetics discriminate between synaptic and tonic GABAA receptors. Effects on both phasic and tonic receptors combined to depress circuit responses produced by thiopental, whereas only effects on synaptic GABA receptors appeared to play an important role for isoflurane. Together with the other known sites of action for these two anesthetics, our results support a multisite, agent-specific mechanism for anesthetic actions.

IMPLICATIONS: We demonstrate that anesthetics can discriminate between different types of γ-aminobutyric acid receptors. This finding provides impetus to develop more selective agents for these receptors in the hope that we can increase the safety, increase the effectiveness and reduce unwanted side effects for new anesthetics.

From the Department of Anesthesia, Stanford University School of Medicine, Stanford, California.

Accepted for publication September 29, 2008.

Supported provided by NIH NIGMS and NIDA.

Reprints will not be available from the author.

Address correspondence to M. Bruce MacIver, MSc, PhD, Department of Anesthesia, Stanford University School of Medicine, SUMC S288, Stanford, CA 94305-5117. Address e-mail to maciver@stanford.edu.

General anesthetics have been shown to depress neuronal responses throughout the central nervous system and some of this depression appears to result from actions at γ-aminobutyric acid (GABAA)-mediated inhibitory synapses via postsynaptic ionotropic chloride channels.1,2 GABAA receptor/channels (GABARs) exist at synapses, clustered beneath active zones of nerve terminals that release GABA and also in perisynaptic locations where they can sense the spillover of GABA from nearby synapses.3,4 There appear to be many types of GABAA receptors expressed, made up of different combinations of subunits which impart distinct biophysical, physiological, and pharmacological characteristics to each receptor/channel.5 In hippocampal CA1 neurons, for example, there are traditional fast GABARs found at synapses on the cell bodies of pyramidal cells and these are thought to be comprised of α 1–2, β 2, and γ 2 subunits.6 They exhibit rapid activation (approximately 1 ms) and fast desensitizing responses to GABA and likely function to control rapid feedback inhibition of pyramidal neurons.7,8 Slow synaptic GABAA receptors are localized to dendritic synapses and are thought to include β 3 subunits and exhibit slower onset (>3 ms) and longer decay time kinetics.9,10 These slow synaptic receptors are ideally suited to modulate synaptic plasticity and dendritic integration of CA1 neurons.11 A third type of receptor appears to be more diffusely distributed, responding to low ambient levels of GABA not associated with synapses, perhaps located in extrasynaptic cellular regions.12 These receptors generate tonic chloride currents that show little evidence of desensitizing.5 Tonic receptors expressed by CA1 neurons respond to low μM concentrations of GABA and appear to be comprised of receptors that include α 5 subunits.13,14

Several recent studies suggest that tonic GABARs could be important targets for general anesthetics and alcohols.13,15–20 The purpose of the present study was to compare the involvement of tonic versus synaptic GABAA receptors in the depressant effect produced by a barbiturate, thiopental, and an inhaled anesthetic, isoflurane. Synaptic GABAA receptors were selectively blocked using gabazine,15,16,21 and any additional involvement of tonic receptors was determined by blocking remaining chloride channels with picrotoxin (Fig. 1). Two measures of neuronal depression were used. The first tested individual nerve cell responses to depolarizing current injection, as a measure of effects on intrinsic excitability. The second measured effects on population spike responses to assess whether synaptic and circuit level depression involved anesthetic-induced tonic GABAA receptor activation.

Figure 1

Figure 1

Back to Top | Article Outline

METHODS

Brain slice preparation methods have been described in detail.22,23 In short, standard transverse hippocampal slices (0.4 μm) from mature Sprague-Dawley rats (P28–P40, most P33–36) were prepared using a vibratome. All procedures conformed to National Institute of Health guidelines for the care and use of animals and were approved by Stanford University’s animal use IRB.

Back to Top | Article Outline

Electrophysiology

Standard visualized slice procedures were used. All whole-cell recordings were from CA1 pyramidal neurons in the cell body layer (stratum pyramidale) with seal resistances of at least 3 GOhm. Input resistances ranged between 150 and 400 Mohm, and there were no obvious differences between cells that responded to thiopental or isoflurane. Every neuron displayed stable spiking and input resistance during control recordings (±2%–4%). Throughout current clamp experiments, sets of current steps were applied repetitively at fixed intervals (typically, six steps/set: two hyperpolarizing, one at 0 pA, and three depolarizing; 1 set/min). In long duration (>2 hr) current clamp experiments, we found that neurons tolerated protocols using small numbers of depolarizing current steps repetitively applied (three/set) at these low repetition rates. We did not apply tonic currents to adjust resting potentials. All experiments were conducted at room temperature (22°C–24°C) using a submersion chamber and >95% of tubing was Teflon to minimize drug binding or permeation loss. Using continuous perfusion of artificial cerebrospinal fluid (ACSF) at 2–3 mL/min, complete bath replacement took approximately 2 min, as measured by dye exchange. Each slice was used for only a single experiment. The following external (ACSF) was used (in mM): 124 NaCl, 3.5 KCl, 1.25 NaH2PO4, 2 MgSO4, 2 CaCl2, 26 NaHCO3, 10 glucose; it was bubbled with 95% O2/5% CO2 to reach pH 7.4.

We used standard whole-cell methods (pipette resistance 4–8 Mohm). A potassium gluconate-based internal solution was used (in mM): 100 K-gluconate, 10 EGTA, 5 MgCl2, 40 HEPES, 2 Na2ATP, 0.3 NaGTP (pH 7.2 with KOH). All voltage values were corrected for electrode offset and junction potentials. All recordings were established for >15 min before recording baseline data and recordings showing unstable response properties (>4% variability) were not used.

Whole-cell DC current-evoked spiking data were collected using PClamp 8.0 (Axon Instruments, Union City, CA) and analyzed using custom procedures written in Igor Pro 5.0 (Wavemetrics, Oswego, OR).

Population spike responses were generated by stimulating Schaffer-collateral fibers using bipolar tungsten microelectrodes (10 MOhm FHC, Bowdoin, ME) driven by a Grass s48 stimulator (0.22–0.25 ms pulse width, 5–8 V). Glass micropipette recording electrodes filled with ACSF (1.0–2.0 KOhm) were placed at the boarder of stratum oriens and stratum pyramidale to monitor CA1 neuron population spike discharge amplitudes. Only slices that exhibited population spike responses of at least 10 mV were used for experiments. Signals were amplified (1000×, Brownlee CO Santa Clara, CA), digitized (National Instruments USB 6009) and stored (MacBook Pro, IgorPro, Wavemetrics). Analysis was performed using IgorPro and two-tailed Student’s t-test or repeated measures ANOVA with Tukey test were used to evaluate statistical significance (P < 0.05 or below; GraphPad software, Prism, San Diego, CA).

Back to Top | Article Outline

Compounds

All chemicals for ACSF were reagent grade or better and obtained from Sigma/RBI. Thiopental was obtained from Sigma-Aldrich (Saint Louis, MO) and made fresh daily as a 12.5 mM stock in distilled water and NaOH. Isoflurane was obtained from Hospira (Novaplus, Lake Forest, IL) and applied using a calibrated vaporizor (Isotec, SurgiVet, Waukesha, WI). Gas phase isoflurane concentrations were measured using a Datex 254 agent monitor (Puriton-Bennett, Pleasanton, CA), and solution concentrations were verified using a custom electrochemical measure as previously described.22

Back to Top | Article Outline

RESULTS

Thiopental Depressed CA1 Neuron Action Potential Discharge Via Tonic GABAA Receptors

The barbiturate anesthetic, thiopental, produced a rapid and reversible depression of CA1 neuron discharge activity (Fig. 2). Action potential responses to low intensity current injection (40 pA) were completely blocked and responses to higher intensity current pulses were strongly depressed. When synaptic GABA receptors were antagonized with the competitive receptor antagonist gabazine, there was no apparent reversal of thiopental-induced depression. In contrast, application of picrotoxin in the continued presence of thiopental and gabazine produced a full reversal of the barbiturate-induced depression. These findings were highly significant, comparing thiopental-induced depression to control discharge levels and for the picrotoxin-induced reversal of thiopental’s effect (Fig. 2).

Figure 2

Figure 2

The barbiturate-induced depression of action potential discharge was accompanied by an increase in membrane conductance, measured using hyperpolarizing current pulse injections (Fig. 2). Thiopental increased membrane conductance by approximately 100%, and this increase was not antagonized by blocking synaptic GABA receptors with gabazine. The increase in membrane conductance, however, was reversed by blocking tonic GABA-gated chloride channels with picrotoxin.

Back to Top | Article Outline

Isoflurane-Induced Depression of CA1 Neuron Excitatibility

In contrast to the strong depressant effects produced by thiopental, isoflurane did not alter action potential discharge responses to depolarizing current in CA1 neurons (Fig. 3). Even in the presence of high concentrations (1.0 rat MAC; 350 μM) sufficient for surgical levels of anesthesia, there was little change evident for low stimulus intensity responses (40 pA) and no apparent effect for responses to higher stimulation levels. Similarly, there was no apparent change in membrane conductance produced by isoflurane.

Figure 3

Figure 3

Back to Top | Article Outline

Synaptic and Tonic GABA Receptor Inhibition of CA1 Neurons

Gabazine alone produced a small, nonsignificant at n = 5, increase in action potential discharge frequency (1.4 ± 1.1 Hz; P > 0.1). Picrotoxin applied in the continuing presence of gabazine (n = 5) or alone (n = 5) produced a significant, but modest, increase in discharge frequency (1.5 ± 0.6 Hz; P < 0.01, pooled data). Thus, under resting conditions, there was only a small influence of synaptic or tonic GABA-mediated inhibition on the excitability of CA1 neurons. A more pronounced involvement of GABA inhibition was evident for synaptically driven excitation, measured using the Schaffer-collateral to CA1 neuron population spike response (Fig. 4). Blocking synaptic GABA receptors with gabazine produced a significant increase in population spike amplitudes (116% ± 9% of control; P < 0.005, n = 5). Subsequent addition of picrotoxin produced a small, but nonsignificant, further increase in population spike amplitudes, indicating little additional tonic GABA-mediated inhibition. The most dramatic effect was the appearance of additional population spikes after block of synaptically mediated inhibition with gabazine (Fig. 4), with no apparent change with the addition of picrotoxin. Thus, under synaptically driven excitation, both feedforward and feedback GABA-mediated inhibition appears to limit the discharge of CA1 neurons, with little involvement of tonic GABA receptors.

Figure 4

Figure 4

Back to Top | Article Outline

Thiopental-Induced Depression of Synaptically Evoked Discharge

Thiopental produced a brief increase in population spike amplitudes followed by a profound depression of discharge (Fig. 5). Blocking synaptic GABA receptors with gabazine significantly reversed the thiopental-induced depression (from 0 to 66% ± 8% of control; P < 0.001, n = 5). Subsequent application of picrotoxin fully reversed the remaining thiopental-induced depression (to 111% ± 10% of control, P < 0.001, n = 5). This indicated that both synaptic and tonic GABA receptors contribute to the depressant effects of thiopental for synaptically driven CA1 neuron discharge.

Figure 5

Figure 5

Back to Top | Article Outline

Isoflurane-Induced Depression of Synaptically Evoked Discharge

Isoflurane produced only a depression of population spike discharge of CA1 neurons (Fig. 6). The onset kinetics for depression was considerably faster than that produced by thiopental (Tau 1/2 of 3.8 min for isoflurane vs 14.6 min for thiopental). A slower component of depression was also evident for isoflurane that was only revealed after application of the GABA antagonists. This indicates that 1 minimum alveolar anesthetic concentration (MAC) isoflurane acts at an additional, non-GABA, site, which exhibits much slower onset kinetics. Gabazine reversed the isoflurane-induced depression to almost 40% of control population spike values, indicating that a significant enhancement of synaptic GABAR-mediated inhibition contributes to this depression (P < 0.001; n = 5). The addition of picrotoxin, to block residual tonic GABAR-mediated inhibition, had no effect (Fig. 6). Previous studies suggested that tonic GABARs might be more sensitive to low concentrations of isoflurane, in the amnesic range of 0.25–0.3 rat MAC.17 To test this, we repeated the population spike depression experiments using 90 μM of isoflurane (approximately 0.25 MAC). This low concentration of isoflurane depressed responses to 60% of control amplitudes with a Tau 1/2 of 9.3 min. Blocking synaptic GABARs with gabazine produced a 11% ± 7.3% reversal of the isoflurane-induced population spike depression (Fig. 6, bottom, P < 0.01). Blocking residual tonic GABARs with picrotoxin did not produce any further reversal. Thus, tonic GABARs do not appear to play a role in depression of synaptically evoked CA1 neuron discharge for either high or low concentrations of isoflurane, but synaptic GABARs contribute significantly to population spike depression at both concentrations. These results, together with the effects produced by thiopental, are summarized in Figure 7.

Figure 6

Figure 6

Figure 7

Figure 7

Back to Top | Article Outline

DISCUSSION

Thiopental acted mainly to enhance phasic GABAA receptor-mediated inhibition, but significant effects were also seen on tonic receptors. Isoflurane, in contrast, enhanced phasic GABAA inhibition but did not appear to enhance tonic GABAA receptors to depress CA1 neuron synaptically evoked discharge. Thus, these two anesthetics can readily discriminate between tonic and phasic GABA receptors. This adds an additional level of complexity to the multiple sites of action previously shown to exist for these two anesthetics by demonstrating that selective interactions occur even among tonic versus phasic GABA receptors.1,6,13

Our results confirm earlier work indicating that GABAA receptors are important targets for both IV and volatile anesthetics1 acting on CA1 neurons23 and other neuron types throughout the brain.24 Recent studies have suggested that tonic receptors may be more important mediators of anesthetic-induced neuronal depression than are phasic receptors.13–18 This has been based largely on observations that tonic receptors appear to be quite sensitive to low concentrations of anesthetics and could be expected to contribute a larger charge transfer or shunting inhibition because of the continuous, nondesensitizing nature of tonic receptors.13 We provide the first quantitative comparison of anesthetic effects at phasic and tonic GABAA receptors using functional measures of neuronal depression in a well-characterized synaptic circuit, the Schaffer-collateral to CA1 neuron pathway. Surprisingly, even though isoflurane can enhance tonic GABAA-mediated currents in these neurons,14,15,17 this did not manifest as a major effect contributing to either direct current-evoked discharge (Fig. 3) or synaptically evoked discharge of CA1 neurons (Figs. 6 and 7). Instead, isoflurane appeared to depress the Schaffer-collateral to CA1 neuron circuit through a combination of effects on phasic GABAA receptors (Fig. 7) and by depressing glutamate-mediated excitatory synaptic inputs, together with additional enhancement non–GABA-mediated inhibitory currents as previously reported.23 Apparently, the isoflurane-induced enhancement of tonic currents in hippocampal cells is not strong enough to alter the discharge properties of these neurons. Perhaps this is because volatile anesthetics do not directly activate the receptors like IV anesthetics do, therefore, in the absence of high ambient GABA concentrations, these drugs are relatively ineffective. If this is true, then tonic GABAA receptors could play a larger role in vivo where higher levels of background GABA might pertain. The IV drug thiopental, in contrast, clearly acted through enhancing both tonic and phasic GABAA receptors to depress CA1 neuron discharge (Fig. 7), and a major component of this depression appeared to involve postsynaptic tonic GABAA receptors (Fig. 2). This is similar to the effect produced by propofol using direct current-evoked discharge as a functional measure of anesthetic-induced depression of discharge.16,25 Of course, direct current-evoked discharge only provides a functional measure for possible tonic receptor contributions at or near the cell body/spike initiation region of neurons. This is why we also tested for a functional depression of synaptically evoked discharge using the population spike measure. The population spike response should be sensitive to tonic GABAA current activation throughout the dendritic region, where shunting of excitatory currents could occur, as well as other membrane regions associated with spike initiation.

It is likely that anesthetic actions at tonic GABAA receptors will contribute more or less to functional depression of neuronal discharge depending on the particular neuron type, receptor subtypes expressed, and/or subcellular distribution of tonic receptors. For example, Jia et al.25 have recently found that isoflurane can significantly depress direct current-evoked discharge of ventrobasal thalamic neurons expressing α 4 containing tonic GABAA receptors. Although this depression was only partial (approximately 25%), it appeared to add to enhanced synaptic GABAA currents in these cells to inhibit firing activity. We may yet discover neurons that can be entirely depressed by isoflurane-induced enhanced tonic currents, but even ventrobasal thalamic neurons appear to be depressed by actions involving several additional sites.24,25

In summary, we provide the first quantitative comparison of anesthetic actions on synaptic and tonic GABAA receptors to demonstrate an agent-specific ability to discriminate between these sites of action.

Back to Top | Article Outline

REFERENCES

1. Tanelian DL, Kosek P, Mody I, MacIver MB. The role of the GABAA receptor/chloride channel complex in anesthesia. Anesthesiology 1993;78:757–76
2. Zeller A, Arras M, Jurd R, Rudolph U. Mapping the contribution of beta3-containing GABAA receptors to volatile and intravenous general anesthetic actions. BMC Pharm 2007;7:2
3. Wei W, Zhang N, Peng Z, Houser CR, Mody I. Perisynaptic localization of delta subunit-containing GABA(A) receptors and their activation by GABA spillover in the mouse dentate gyrus. J Neurosci 2003;23:10650–61
4. Mody I, Glykys J, Wei W. A new meaning for “Gin & Tonic”: tonic inhibition as the target for ethanol action in the brain. Alcohol 2007;41:145–53
5. Mody I, Pearce RA. Diversity of inhibitory neurotransmission through GABA(A) receptors. Trends Neurosci 2004;27:569–75
6. Prenosil GA, Schneider Gasser EM, Rudolph U, Keist R, Fritschy JM, Vogt KE. Specific subtypes of GABAA receptors mediate phasic and tonic forms of inhibition in hippocampal pyramidal neurons. J Neurophys 2006;96:846–57
7. Banks MI, Pearce RA. Kinetic differences between synaptic and extrasynaptic GABA(A) receptors in CA1 pyramidal cells. J Neurosci 2000;20:937–48
8. Pouille F, Scanziani M. Routing of spike series by dynamic circuits in the hippocampus. Nature 2004;429:717–23
9. Pearce RA. Physiological evidence for two distinct GABAA responses in rat hippocampus. Neuron 1993;10:189–200
10. Ramadan E, Fu Z, Losi G, Homanics GE, Neale JH, Vicini S. GABA(A) receptor beta3 subunit deletion decreases alpha2/3 subunits and IPSC duration. J Neurophys 2003;89:128–34
11. Banks MI, White JA, Pearce RA. Interactions between distinct GABA(A) circuits in hippocampus. Neuron 2000;25:449–57
12. McDonald LM, Sheppard WF, Staveley SM, Sohal B, Tattersall FD, Hutson PH. Gaboxadol, a selective extrasynaptic GABA(A) agonist, does not generalise to other sleep-enhancing drugs: a rat drug discrimination study. Neuropharmacology 2007;52:844–53
13. Orser BA. Extrasynaptic GABAA receptors are critical targets for sedative-hypnotic drugs. JCSM 2006;2:S12–8
14. Caraiscos VB, Elliott EM, You-Ten KE, Cheng VY, Belelli D, Newell JG, Jackson MF, Lambert JJ, Rosahl TW, Wafford KA, MacDonald JF, Orser BA. Tonic inhibition in mouse hippocampal CA1 pyramidal neurons is mediated by alpha5 subunit-containing gamma-aminobutyric acid type A receptors. PNAS 2004;101:3662–7
15. Bai D, Zhu G, Pennefather P, Jackson MF, MacDonald JF, Orser BA. Distinct functional and pharmacological properties of tonic and quantal inhibitory postsynaptic currents mediated by gamma-aminobutyric acid(A) receptors in hippocampal neurons. Mol Pharm 2001;59:814–24
16. Bieda MC, MacIver MB. Major role for tonic GABAA conductances in anesthetic suppression of intrinsic neuronal excitability. J Neurophys 2004;92:1658–67
17. Caraiscos VB, Newell JG, You-Ten KE, Elliott EM, Rosahl TW, Wafford KA, MacDonald JF, Orser BA. Selective enhancement of tonic GABAergic inhibition in murine hippocampal neurons by low concentrations of the volatile anesthetic isoflurane. J Neurosci 2004;24:8454–8
18. Wei W, Faria LC, Mody I. Low ethanol concentrations selectively augment the tonic inhibition mediated by {delta} subunit-containing GABAA receptors in hippocampal neurons. J Neurosci 2004;24:8379–82
19. Cope DW, Hughes SW, Crunelli V. GABAA receptor-mediated tonic inhibition in thalamic neurons. J Neurosci 2005;25:11553–63
20. Chandra D, Jia F, Liang J, Peng Z, Suryanarayanan A, Werner DF, Spigelman I, Houser CR, Olsen RW, Harrison NL, Homanics GE. GABAA receptor alpha 4 subunits mediate extrasynaptic inhibition in thalamus and dentate gyrus and the action of gaboxadol. PNAS 2006;103:15230–5
21. McCartney MR, Deeb TZ, Henderson TN, Hales TG. Tonically active GABAA receptors in hippocampal pyramidal neurons exhibit constitutive GABA-independent gating. Mol Pharm 2007;71:539–48
22. Hagan CE, Pearce RA, Trudell JR, MacIver MB. Concentration measures of volatile anesthetics in the aqueous phase using calcium sensitive electrodes. J Neurosci Methods 1998;81:177–84
23. Pittson S, Himmel AM, MacIver MB. Multiple synaptic and membrane sites of anesthetic action in the CA1 region of rat hippocampal slices. BMC Neurosci 2004;5:52
24. Hemmings HC Jr, Akabas MH, Goldstein PA, Trudell JR, Orser BA, Harrison NL. Emerging molecular mechanisms of general anesthetic action. TIPS 2005;26:503–10
25. Jia F, Yue M, Chandra D, Homanics GE, Goldstein PA, Harrison NL. Isoflurane is a potent modulator of extrasynaptic GABA(A) receptors in the thalamus. J Pharmacol Exp Ther 2008;324:1127–35
© 2009 International Anesthesia Research Society