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Abstracts of Posters Presented at the International Anesthesia Research Society; 72nd Clinical and Scientific Congress; Orlando, FL; March 7-11, 1998: Critical Care Anesthesia

FIBRIN MONOMERS (FM) IN MINI PIGS DURING AND AFTER PARAOXON EXPOSURE

Toomes, M.; Petroianu, G. MD; Werth, WA; Rufer, R. MD

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doi: 10.1097/00000539-199802001-00155
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Abstract S156

INTRODUCTION: Paraoxon (POX) can cause thrombosis, embolisation and death. There is little agreement about the extent and nature of the coagulation abnormality induced [1].

PURPOSE OF STUDY: Establish the effect of POX on the coagulation of mini pigs as assessed by measurements of the FM concentration.

METHODS AND MATERIALS: Anesthezised male mini pigs (ZESTRANI-method) were intubated and mechanically ventilated [2]. Montoring: BP continuous (carotid), CVP (internal jugular), capnometry, pulsoximetry, arterial and venous blood gases, hematocrit (Hct). Fluids, atropine, and magnesium were administered through the external jugular vein to maintain Hct and vital parameters close to 75% of the baseline values. Mini pigs received POX (0.3, 1, 3, 9, 27 and 81 mg kg-1 BW-1 respectively) or saline (control animal). Venous blood (internal jugular) was obtained every ten minutes during and every thirty minutes for two hours after POX-infusion. For measurements of FM the Berichrom[registered sign] FM test (Behringwerke AG, 35001 Marburg) was used. The measured values were expressed in percent of the baseline values.

RESULTS: No increase in the FM concentration was detected in control- or 0.3, 1, 3, 9 and 27 mg kg-1 BW-1 POX-animals. At a dose of 81 mg POX kg-1 BW-1 a marked increase of FM was noticed (Figure 1).

F1-155
Figure 1:
POX was infused from t0 to t50

DISCUSSION: An increase in FM concentration is a very sensitive indicator of an activated coagulation system. It was shown by earlier measurements of FM concentrations that POX has no in vitro coagulation activating effects [3]. The activation of coagulation seen after application of 81 mg POX kg-1 BW-1 is probably due to a massive release of catecholamines from the adrenals. As the adrenals are activated by acetylcholine (ACh) and POX is an ACh esterase inhibitor, high dose POX can cause a phaeochromocytoma-like reaction with i.a. activation of the coagulation.

REFERENCES

1. Arch Pharmacol 353 (196): R 127 # 479
2. J Vet Med A 43 (1996): 415-421
3. Toxicol 119 (1997): 167-173
© 1998 International Anesthesia Research Society