Review ArticlesWhole Slide Imaging: Technology and ApplicationsHanna, Matthew G. MD*; Parwani, Anil MD, PhD, MBA†; Sirintrapun, Sahussapont Joseph MD*Author Information *Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY †Department of Pathology, Ohio State University Wexner Medical Center, Columbus, OH The authors have no funding or conflicts of interest to disclose. Reprints: Matthew G. Hanna, MD, Department of Pathology, Memorial Sloan Kettering Cancer Center, 1275 York Ave, New York, NY 10065 (e-mail: email@example.com). Advances In Anatomic Pathology: July 2020 - Volume 27 - Issue 4 - p 251-259 doi: 10.1097/PAP.0000000000000273 Buy Metrics Abstract Pathology has benefited from advanced innovation with novel technology to implement a digital solution. Whole slide imaging is a disruptive technology where glass slides are scanned to produce digital images. There have been significant advances in whole slide scanning hardware and software that have allowed for ready access of whole slide images. The digital images, or whole slide images, can be viewed comparable to glass slides in a microscope, as digital files. Whole slide imaging has increased in adoption among pathologists, pathology departments, and scientists for clinical, educational, and research initiatives. Worldwide usage of whole slide imaging has grown significantly. Pathology regulatory organizations (ie, College of American Pathologists) have put forth guidelines for clinical validation, and the US Food and Drug Administration have also approved whole slide imaging for primary diagnosis. This article will review the digital pathology ecosystem and discuss clinical and nonclinical applications of its use. Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.