Original ArticlesUsing Skin Gene Markers for Estimating Early Postmortem Interval at Different TemperaturesAli, Mona Mohamed PhD*; Ibrahim, Samah Fathy PhD*†; Fayed, Amel Ahmed PhD†Author Information From the *Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Cairo University, Egypt; and †College of Medicine, Princess Nourah Bint-Abdulrahman University, Saudi Arabia. Manuscript received February 27, 2017; accepted June 12, 2017. The authors report no conflict of interest. Reprints: Samah Fathy Ibrahim, PhD, Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Cairo University, Cairo 11562, Egypt. E-mail: email@example.com. The American Journal of Forensic Medicine and Pathology: December 2017 - Volume 38 - Issue 4 - p 323-325 doi: 10.1097/PAF.0000000000000337 Buy Metrics Abstract Many researches document long-term RNA persistence in a variety of tissues and its applicability in estimating the postmortem interval (PMI). Skin-specific mRNA marker, late cornified envelope 1C (LCE1C), was used to identified skin samples. Before using the LCE1C in criminal casework, its persistence and applicability for estimating PMI in different temperatures were tested. Twelve skin samples were collected from 6 patients, and 6 samples were kept at 24°C and others were kept at 40°C for 5 days. The expression levels of LCE1C mRNA are serially detected and quantified using real-time polymerase chain reaction. The expression levels of LCE1C were decreased with increasing the time interval in time-dependent manner, whereas changing the surrounding temperatures did not show any statistical significance. These results could suggest using LCE1C in estimation of PMI. Moreover, these encourage investigators and crime laboratories to know environmental conditions before interpreting the results. Copyright © 2017 Wolters Kluwer Health, Inc. All rights reserved.