Original ArticlesExpression of Glucose-Regulated Protein 78 and miR-199a in Rat Brain After Fatal Ligature StrangulationFeng, Xueying MD*†; Zhang, Dongchuan MD*; Gong, Qingjin MD*; Zhang, Zhiyong MD*‡; Quan, Li MD, PhD*Author Information From the *Department of Forensic Pathology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou; †Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai; and ‡Forensic Science Center, Shunde Branch of Foshan Public Security Bureau, Foshan, China. Manuscript received August 26, 2016; accepted December 6, 2016. Supported by the Natural Science Foundation of Guangdong Province (grant no. 2015A030313110) and Shanghai Key Laboratory of Forensic Medicine (grant no. KF1503). The authors report no conflict of interest. Reprints: Li Quan, MD, PhD, Department of Forensic Pathology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2 Rd, Guangzhou 510080, China. E-mail: firstname.lastname@example.org. The American Journal of Forensic Medicine and Pathology: March 2017 - Volume 38 - Issue 1 - p 78-82 doi: 10.1097/PAF.0000000000000298 Buy Metrics Abstract The roles of endoplasmic reticulum (ER) stress and microRNA in the brain tissue after fatal mechanical asphyxia have not been clearly elucidated. We examined the expression of glucose-regulated protein 78 (GRP78), the key regulator of unfolded protein response, and miR-199a in the brain tissues of rats subjected to fatal ligature strangulation to understand the roles of ER stress and microRNA in ligature strangulation. The expressions of GRP78 and miR-199a in rat cortex, hippocampi, and midbrain were measured by immunohistochemistry and Western blot analysis in a rat model of ligature strangulation. Furthermore, the levels of miR-199a-3p and miR-199a-5p were detected by real-time fluorescent quantitative polymerase chain reaction. Glucose-regulated protein 78 was highly expressed in the cortex and midbrain in the ligature strangulation group (P < 0.01) when compared with the control group. The expression of GRP78 in the hippocampi showed no significant difference between the 2 groups. miR-199a-3p in the cortex and midbrain was significantly down-regulated in the ligature strangulation group (P < 0.01). However, miR-199a-5p in each brain region showed no significant difference between the 2 groups. In conclusion, ER stress was involved in the physiological and pathological processes of ligature strangulation. Furthermore, upstream miR-199a may play an important regulatory role in mechanical asphyxia. Copyright © 2017 Wolters Kluwer Health, Inc. All rights reserved.