Original ArticleHighly Sensitive HLA-DNA Typing From Formalin-Fixed and Paraffin-Embedded Tissue SamplesOta, Masao PhD*; Shimada, Kazunori BS†; Asamura, Hideki MD*; Katsuyama, Yoshihiko PhD‡; Fukushima, Hirofumi MD*Author Information From the *Department of Legal Medicine, Shinshu University School of Medicine, Matsumoto, Japan; †Genome Science Laboratories, Co, Ltd, Fukushima, Japan; and ‡Department of Pharmacy, Shinshu University Hospital, Matsumoto, Japan. Manuscript received July 10, 2005; accepted August 19, 2005. This work was supported in part by research grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan 09670434 (Grand-in-Aid for Scientific Research (C)). Reprints: Masao Ota, PhD, Department of Legal Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan. E-mail: [email protected]. The American Journal of Forensic Medicine and Pathology: December 2006 - Volume 27 - Issue 4 - p 347-351 doi: 10.1097/01.paf.0000233558.79449.0c Buy Metrics Abstract Nucleotide sequences have been determined for more than 1700 different alleles at the core of the human leukocyte antigen (HLA) system. The highly polymorphic character of these genes affects adaptive immune response and is also useful for forensic applications. HLA typing from formalin-fixed and paraffin-embedded tissue provides abundant useful information for both clinical settings and forensic investigations. This study, which investigated the potential use of DNA from formalin-fixed and paraffin-embedded tissue samples in an HLA PCR sequence-specific primer and probe (SPP) system, showed that tissue fixed in formalin for less than 3 days and embedded in paraffin can serve as a useful source of DNA for PCR-SPP typing kits. © 2006 Lippincott Williams & Wilkins, Inc.