Original StudyMelanocytes Pattern in the Normal Nail, With Special Reference to Nail Bed MelanocytesPerrin, Christophe MD*,†; Michiels, Jean-F. MD*; Boyer, Julien MD*; Ambrosetti, Damien MD* Author Information *Laboratoire Central d'Anatomie Pathologique, Hôpital L. Pasteur, University of Nice, Nice, France; and †Nail's Dermatology Consultations, Cannes, France. Correspondence: Christophe Perrin, MD, Laboratoire Central d'Anatomie Pathologique, Hôpital L Pasteur, 30, Avenue Voie Romaine- CS 51069, 06001 Nice Cedex 1, France (e-mail: [email protected]). The authors declare no conflicts of interest. The authors state that IRB approval was not required. The manuscript is original, has not been published previously, and is not under consideration for publication elsewhere. The American Journal of Dermatopathology: March 2018 - Volume 40 - Issue 3 - p 180-184 doi: 10.1097/DAD.0000000000000940 Buy Metrics Abstract Previous studies of the density of melanocytes in the normal nail bed have had conflicting results. This is unfortunate because knowing the normal values might help the difficult distinction between a benign subungual melanotic macule and an early melanoma in situ. Five specimens of normal nail unit were analyzed. On hematoxylin and eosin–stained sections the melanocytes were undetectable. We defined the melanocyte count (MC) as the number of melanocytes per 1-mm stretch of nail epithelium. The mean MC for nail matrix was 6.86 with a range of 4–14. The melanocytes were irregularly scattered in the basal and suprabasilar layer of the matrix epithelium. Abundant and uneven cytoplasmic dendrites were focally observed in the matrix. The MC for the nail bed ranged from 0 to 5 with a mean of 0.43. The melanocytes were restricted to the basal layer with thin cytoplasmic dendrites. Two cases showed a complete absence of melanocytes in the nail bed. In the ventral portion of the proximal nail fold, called the eponychium, the MC ranged between 0 and 5/mm with a mean of 2.27/mm. In conclusion, we discovered foci in normal nail beds, in which the melanocytic density can be relatively high and reach the level seen in the matrix. HMB45 is more sensitive than Microphtalmia-associated transcription factor (MITF) for the evaluation of intraepithelial melanocytic density of the nail unit. If MITF is used alone in the nail bed, its weak sensitivity may result in a false-negative interpretation and may be wrongly reassuring in the evaluation of early melanomas. On hematoxylin and eosin sections, basal and suprabasal nail keratinocytes are sometimes crowded, showing oval or elongated dark-staining nucleus and a clear cytoplasm and mimics a melanocytic proliferation. On HMB45 or Melan A staining, the morphology and the distribution of the dendrites of matrical melanocytes can mimic the dendritic pattern usually described in acral melanoma. Therefore, the interpretation of nail melanocytic atypia must be prudent. Copyright © 2017 Wolters Kluwer Health, Inc. All rights reserved.