USING FLOW CYTOMETRIC ANALYSIS, we followed Changes in a patient's bone marrow which was infiltrated with cells resistant to chemotherapy Samples taken prior to, during and after treatment with dianhydrogalactitol (2 days). adrosine arabinoside and hydroxyurea (2 days). and Adraaycin (2 days) contained an aneuploid population of cells which exhibited changes in ploidy, viability and distribution in cell cycle compartment. The restricted changes in cell ploidy and viability of tumor cells encountered as well as limited increases in S phase on day 4 and C2 phase on day 6 with rebound on day 9 of C1 phase cells suggest the tumor to be sensitive to dianhydrogalactitol but not to the other agents used.
Memorial Sloan-Kettring Cancer Center, New York, New York.