Extraction of 20(S)-Ginsenoside Rg₂ from Cultured Panax notoginseng Cells In Vitro Stimulates Human Umbilical Cord Vein Endothelial Cell Proliferation

Abstract

From the cultured cells of Panax notoginseng, a kind of ginseng saponin—ginsenoside Rg₂, was separated and purified, and its structure was elucidated as 6-O-[α-L-rhamnose (l→2)-β-D-glucopyranosyl]3β, 12β, 20(S)-trihydroxydammar-24-ene [20(S)-ginsenoside Rg₂]. Moreover, its bioactivity stimulates human umbilical cord vein endothelial cell proliferation; the growth of the cells treated with 20(S)-ginsenoside Rg₂ was 14-fold higher than that of untreated ones, and the level of the cell's plasminogen activator (PA) was also twofold higher.