Original ArticlesLMO2-negative Expression Predicts the Presence of MYC Translocations in Aggressive B-Cell LymphomasColomo, Luis MD*,†; Vazquez, Ivonne MD*; Papaleo, Natalia MD*; Espinet, Blanca MD*; Ferrer, Anna MD*; Franco, Catalina MD*; Comerma, Laura MD*; Hernandez, Silvia MD*; Calvo, Xavier MD*; Salar, Antonio MD*; Climent, Fina MD‡; Mate, José Luis MD§; Forcada, Pilar MD∥; Mozos, Anna MD¶; Nonell, Lara MD#; Martinez, Antonio MD†; Carrio, Anna MD†; Costa, Dolors MD†; Dlouhy, Ivan MD†; Salaverria, Itziar MD†; Martin-Subero, Jose Ignacio MD†; Lopez-Guillermo, Armando MD†; Valera, Alexandra MD†,**; Campo, Elias MD†; Grup per l’Estudi dels Limfomes de Catalunya i Balears (GELCAB)Author Information *Department of Pathology, Hematopathology Unit, Hospital del Mar, Institute of Investigacions Mediques-IMIM, University Pompeu Fabra †Department of Pathology, Hematopathology Section, Hospital Clinic, Institute of Investigacions Biomediques Agust Pi Sunyer (IDIBAPS), University of Barcelona ‡Department of Pathology, Hospital de Bellvitge-IDIBELL, Hospitalet de Llobregat ¶Department of Pathology, Hospital de Sant Pau #Microarray Analysis Service, Institute of Investigacions Mediques-IMIM, Barcelona §Department of Pathology, Hospital Germans Trias i Pujol, Badalona ∥Department of Pathology, Hospital Mutua Terrassa, Terrassa, Spain **Institute of Pathology, Locarno, Switzerland Supported by grants from the Comisión Interministerial de Ciencia y Tecnología (CICYT) SAF2015-64885-R and the Generalitat de Catalunya 2009SGR992 (E.C.), from Spanish Ministry of Economy and Competitivity, Fondo de Investigación Sanitaria (FIS), Instituto de Salud Carlos III PI11/01621 (B.E.) and PI10/00366 (A.F.), and grant ACCIO-CIDEM RD08-2-0035 (L.C.). Conflicts of Interest and Source of Funding: E.C. is an ICREA-Academia researcher of the Generalitat de Catalunya. For the remaining authors none were declared. Correspondence: Luis Colomo, MD, Department of Pathology, Hematopathology Section, Hospital del Mar; Passeig Maritim 25-29, Barcelona 08003, Spain (e-mail: [email protected]). The American Journal of Surgical Pathology: July 2017 - Volume 41 - Issue 7 - p 877-886 doi: 10.1097/PAS.0000000000000839 Buy SDC Erratum Metrics Abstract MYC translocation is a defining feature of Burkitt lymphoma (BL), and the new category of high-grade B-cell lymphomas with MYC and BCL2 and/or BCL6 translocations, and occurs in 6% to 15% of diffuse large B-cell lymphomas (DLBCLs). The low incidence of MYC translocations in DLBCL makes the genetic study of all these lymphomas cumbersome. Strategies based on an initial immunophenotypic screening to select cases with a high probability of carrying the translocation may be useful. LMO2 is a germinal center marker expressed in most lymphomas originated in these cells. Mining gene expression profiling studies, we observed LMO2 downregulation in BL and large B-cell lymphoma (LBCL) with MYC translocations, and postulated that LMO2 protein expression could assist to identify such cases. We analyzed LMO2 protein expression in 46 BLs and 284 LBCL. LMO2 was expressed in 1/46 (2%) BL cases, 146/268 (54.5%) DLBCL cases, and 2/16 (12.5%) high-grade B-cell lymphoma cases with MYC and BCL2 and/or BCL6 translocations. All BLs carried MYC translocation (P<0.001), whereas LMO2 was only positive in 6/42 (14%) LBCL with MYC translocation (P<0.001). The relationship between LMO2 negativity and MYC translocation was further analyzed in different subsets of tumors according to CD10 expression and cell of origin. Lack of LMO2 expression was associated with the detection of MYC translocations with high sensitivity (87%), specificity (87%), positive predictive value and negative predictive value (74% and 94%, respectively), and accuracy (87%) in CD10+ LBCL. Comparing LMO2 and MYC protein expression, all statistic measures of performance of LMO2 surpassed MYC in CD10+ LBCL. These findings suggest that LMO2 loss may be a good predictor for the presence of MYC translocation in CD10+ LBCL. Erratum In the article that appeared on page 877 of the July 2017 issue, an affiliation was missing for the second author, Dr. Ivonne Vazquez. In addition to the Department of Pathology, Hematopathology Unit, Hospital del Mar, Institute of Investigacions Mediques-IMIM, University Pompeu Fabra, Dr. Vazquez is also affiliated with Universitat Autonoma de Barcelona. The authors regret this error. The American Journal of Surgical Pathology. 43(3):434, March 2019. Copyright © 2017 Wolters Kluwer Health, Inc. All rights reserved.