Original ArticleApo D in Soft Tissue Tumors A Novel Marker for Dermatofibrosarcoma ProtuberansWest, Robert B MD, PhD*; Harvell, Jeff MD*; Linn, Sabine C MD, PhD*; Lui, Chih Long¶; Prapong, Wijan¶; Hernandez-Boussard, Tina PhD¶; Montgomery, Kelli*; Nielsen, Torsten O MD, PhD†; Rubin, Brian P MD, PhD‡; Patel, Rajiv MD§; Goldblum, John R MD§; Brown, Patrick O MD, PhD¶; van de Rijn, Matt MD, PhD*Author Information From the Departments of *Pathology and ¶Biochemistry and Howard Hughes Medical Institute, Stanford University Medical Center, Stanford, CA; †Department of Pathology and Genetic Pathology Evaluation Centre, Vancouver General Hospital, Vancouver, British Columbia, Canada; ‡Department of Anatomical Pathology, University of Washington Medical Center, Seattle, WA; and §Department of Anatomic Pathology, Cleveland Clinic Foundation, Cleveland, OH. Supported by NIH grants CA85129 and CA84967 and the Howard Hughes Medical Institute. P.O.B. is an Associate Investigator of the Howard Hughes Medical Institute. S.C.L. was a recipient of a Dutch Cancer Society Postdoctoral Research Fellowship. Reprints: Matt van de Rijn, Department of Pathology, Stanford University Medical Center, 300 Pasteur Drive, Stanford, CA 94305 (e-mail: [email protected]). The American Journal of Surgical Pathology: August 2004 - Volume 28 - Issue 8 - p 1063-1069 doi: 10.1097/01.pas.0000126857.86186.4c Buy Metrics Abstract Using gene microarray expression profiling, we previously found that apolipoprotein D (Apo D) was highly expressed in dermatofibrosarcoma protuberans (DFSP). In this study, we confirm that Apo D is highly and relatively specifically expressed in DFSP using immunohistochemistry. A tissue microarray containing 421 soft tissue tumors was constructed and stained with antibodies against Apo D and CD34. Cytoplasmic immunostaining for Apo D was found in 9 of 10 typical DFSPs. In addition, 3 of 3 Bednar tumors and 2 of 3 giant cell fibroblastomas stained in conventional sections. In contrast, Apo D was immunoreactive in only a very small subset of a diverse collection of other soft tissue tumors, including Malignant Fibrous Histiocytoma (MFH), glomus tumor, neurofibroma, and malignant peripheral nerve sheath tumors. Immunostains for Apo D were negative in conventional sections of 16 fibrous histiocytomas, and an additional 12 variants of fibrous histiocytoma. Digital images of all immunohistochemical and hematoxylin and eosin tissue microarray stains are available at the accompanying website (http://microarray-pubs.stanford.edu/tma_portal/apod/). We conclude that Apo D is strongly expressed in DFSPs and neural lesions and may be useful in differentiating DFSP from fibrous histiocytoma. © 2004 Lippincott Williams & Wilkins, Inc.