Although biologics are effective for treating inflammatory bowel diseases (IBDs) (1), up to 30% of patients do not respond (primary nonresponders) and another 50% lose response over time (secondary loss of response [SLR]) (2,3). Subtherapeutic drug concentrations with or without the development of antidrug antibodies (ADA) can explain a substantial portion of these outcomes (4).
Therapeutic drug monitoring (TDM), defined as the measurement of drug concentrations and ADA, has surfaced as an important tool for optimizing biologic therapy (5). Reactive TDM is the evaluation of drug concentration and ADA in the setting of treatment failure and can help facilitate decision-making in both primary nonresponse (PNR) and SLR (6–10). Preliminary data suggest that proactive TDM, defined as the systematic measurement of drug trough concentrations and ADA with dose adaptation to a target drug concentration, can also improve the efficacy of anti–tumor necrosis factor (anti-TNF) therapy (11–19). Proactive TDM may also be used to decrease the dose of infliximab in patients in remission with greater than adequate infliximab concentrations (20–23) or for optimizing infliximab monotherapy as a potential alternative to combination therapy with an immunomodulator (IMM) in select patients (24,25). However, there is debate on when and how to perform TDM in clinical practice.
We aimed to reach a consensus on the role of TDM of biologics in IBD and sought to identify clinically relevant drug concentrations and ADA thresholds to guide physicians on how to better apply TDM in clinical practice.
We applied a modified Delphi method to establish consensus, as previously described (5,26). A comprehensive literature review was performed regarding TDM of biologic therapies in IBD using PubMed and MEDLINE databases. We used the search terms: “inflammatory bowel disease”; “Crohn's disease”; “ulcerative colitis”; “anti-drug antibodies”; “immunogenicity”; “therapeutic drug monitoring”; “point of care assays”; “pharmacokinetics” AND “infliximab” OR “adalimumab” OR “certolizumab pegol” OR “golimumab” OR “vedolizumab” OR “ustekinumab.” Forty-five statements were subsequently formulated (K.P. and A.S.C) on the potential application of TDM in IBD. These statements were grouped into 5 domains: reactive TDM, proactive TDM, general statements regarding TDM, immunogenicity, and drug concentrations to target. The statements, along with literature, were then presented to a panel of 10 gastroenterologists with expertise in IBD and TDM who anonymously rated them on a scale of 1–10 (1 = strongly disagree and 10 = strongly agree). An Expert Consensus Development Meeting was held virtually on October 30, 2020, to review, discuss, refine, and reformulate statements that did not meet criteria for agreement or that were ambiguous. During the meeting, additional statements were proposed. Panelists then confidentially revoted, and statements rated ≥7 by 80% or more of the participants were accepted.
During the virtual Expert Consensus Development Meeting, 8 statements were reworded, 7 new statements were proposed, and 19 statements were rerated. Consensus was finally reached in 48 of 49 statements (Tables 1–5 and see Table 1, Supplementary Digital Content 1, http://links.lww.com/AJG/C132).
Statements that reached consensus regarding the role of reactive TDM are presented in Table 1. Supportive text for these statements (1–9) is provided in Supplementary Digital Content 2 (http://links.lww.com/AJG/C133) (27–44).
Statements that reached consensus regarding the role of proactive TDM are presented in Table 2. Supportive text for these statements is provided below.
Numerous exposure-outcome relationship data from prospective studies and post hoc analyses of randomized controlled trials (RCTs) have shown that higher induction, postinduction, and maintenance anti-TNF drug concentrations are associated with more favorable therapeutic outcomes suggesting a role for proactive TDM for optimizing anti-TNF therapy (3–5). Furthermore, the TAXIT (Trough Concentration Adapted Infliximab Treatment) (RCT), although did not reached its the primary end point, showed that proactive TDM compared with clinically based dosing was associated with lower frequency of undetectable infliximab concentrations and lower risk of relapse (15). In addition, in patients with Crohn's disease (CD) and subtherapeutic drug concentrations, a 1-time dose optimization increased clinical remission rates and decreased C-reactive protein (CRP) (15). The PAILOT (Pediatric Crohn's Disease Adalimumab Level-based Optimization Treatment) RCT demonstrated that proactive dose adjustment of adalimumab when treating pediatric CD was associated with a higher rate of corticosteroid-free clinical remission at all visits from weeks 8–72 when compared with reactive TDM. A proactive TDM approach was also associated with a higher rate of composite sustained corticosteroid-free clinical remission, normal CRP, and normal fecal calprotectin at all time points (16). Furthermore, several retrospective studies for infliximab and 1 for adalimumab have demonstrated that proactive TDM compared with empiric dose optimization and/or reactive TDM was associated with better therapeutic outcomes, such as greater treatment persistence, less need for IBD-related surgery or hospitalization, and lower risk of ADA (11–13,17,18). A recent retrospective multicenter study showed that in patients with an SLR to infliximab who underwent reactive TDM, subsequent proactive TDM after the initial reactive TDM was associated with greater infliximab treatment persistence and fewer IBD-related hospitalizations than reactive TDM alone (19).
Proactive TDM is probably most important in more severely active patients and in those who have higher drug clearance, such as patients during induction therapy and patients with acute severe ulcerative colitis and more severe CD. These patients have a high inflammatory burden, an increased drug clearance, and, therefore, a greater risk of inadequate drug exposure, immunogenicity, and treatment failure (45–48). Another IBD population with a high drug clearance is the pediatric population (49,50).
Proactive TDM can also have an important role when de-escalating therapy (20–23). A prospective study by Amiot et al. (20) suggested that in patients with IBD in clinical remission, de-escalation of infliximab therapy should be performed based on TDM rather than symptoms and CRP. A recent retrospective study of 96 patients with IBD in remission showed that TDM‐based compared with clinically based de-escalation was associated with a decreased risk of relapse (21). Furthermore, it is clinically reasonable to confirm that the trough concentration is still adequate after dose de-escalation. A study from Petitcollin et al. (22) of 91 patients with IBD in deep remission showed that TDM is also useful for following patients after de-escalation. Similarly, proactive TDM should be considered after removal of an immunosuppressive therapy (i.e., azathioprine or methotrexate) (51). A study by Drobne et al. (52) including patients with CD treated with infliximab combination therapy with an IMM showed that a detectable trough infliximab concentration at the time of IMM withdrawal is associated with long-term response. Of note, drug trough concentrations >5 μg/mL at the time of ΙΜΜ withdrawal had a positive predictive value of 100% for not losing response to infliximab (52).
Another possible use of proactive TDM could be to optimize monotherapy in a select group of patients as an alternative to combination therapy with an IMM. In a post hoc analysis of the SONIC (Study of Biologic and Immunomodulator-Naive Patients in CD) RCT, stratification of infliximab concentrations displayed comparable outcomes within each concentration quartile irrespective of concomitant azathioprine, suggesting that combination therapy with infliximab and azathioprine may not be required if adequate drug concentrations of infliximab are attained using proactive TDM (51). Furthermore, 2 recent retrospective studies showed that drug persistence was similar between patients on optimized infliximab monotherapy based on proactive TDM and patients receiving combination therapy (24,25). Lega et al. also showed that in patients undergoing proactive TDM for optimizing infliximab monotherapy compared with those receiving unoptimized infliximab monotherapy, infliximab drug concentrations during maintenance therapy were higher and treatment discontinuation was lower. Moreover, no patient undergoing proactive TDM had antibodies to infliximab (ATI) at first TDM compared with 41% of patients receiving unoptimized infliximab monotherapy (P = 0.002) (24). However, currently, there are no data from RCTs supporting the concept of optimized infliximab monotherapy based on proactive TDM as an alternative to combination therapy with an IMM.
It should be clear that we are not recommending anti-TNF monotherapy over combination therapy with an IMM because unoptimized monotherapy without early and aggressive proactive TDM is not as effective as combination therapy with an IMM and should not be considered. However, proactive TDM-based optimized anti-TNF monotherapy could be considered in a select group of adherent patients based on several factors such as risk of adverse events and patient preference(53–56). Examples include situations where there is concern for increased risk of serious infection or malignancy (54) or when there is no genetic predisposition for immunogenicity (55,56). Proactive TDM for optimizing anti-TNF monotherapy is better than unoptimized anti-TNF monotherapy.
Regarding biologics other than anti-TNF therapies, the only data supporting the role of proactive TDM currently derive exclusively from exposure-response relationship studies showing that higher vedolizumab and ustekinumab concentrations are associated with better therapeutic outcomes (30–44).
General considerations regarding TDM
General statements regarding TDM that reached consensus are presented in Table 3. Supportive text for these statement is provided below and in Supplementary Digital Content 2 (http://links.lww.com/AJG/C133).
Anti-TNF induction therapy.
Several studies have shown an association between higher induction anti-TNF drug concentrations and favorable therapeutic outcomes in IBD, implying that TDM should probably be performed early after treatment initiation. For example, higher infliximab concentrations at weeks 6 and 14 are associated with higher rates of positive clinical outcomes, so checking drug concentrations at these time points is reasonable (3–5). TDM during induction is important because patients during induction have active disease (often characterized by low serum albumin and high baseline CRP levels) and consequently increased drug clearance, putting them at higher risk of inadequate drug exposure, early development of immunogenicity, and treatment failure. (57–62). In addition to ADA, low albumin and high CRP levels are associated with a higher anti-TNF clearance. There is also some evidence that male gender and high body mass index are correlated with lower drug concentration (63). In the prospective PANTS (Personalized anti-TNF therapy in CD) study, low drug concentration at week 14 was independently associated with PNR and nonremission at week 54 for both infliximab and adalimumab. The optimal week 14 drug concentrations associated with remission at weeks 14 and 52 were 7 mg/L for infliximab and 12 mg/L for adalimumab. For both drugs, suboptimal week 14 drug concentrations were associated with immunogenicity, as was the development of ADA with subsequent low drug concentrations (64). In a study by Verstockt et al. (58), patients with low adalimumab concentrations at week 4 (<8.3 μg/mL) were at significantly higher risk to have antibodies to adalimumab (ATA) by week 12 (46.7% vs 13.0%, P = 0.009). The 21.4% of patients who were ATA-positive by week 12 had significantly more frequent dose escalation and experienced sustained clinical benefit less frequently because of PNR or SLR.
Infliximab drug holiday.
In a study from Baert et al. (65) among 128 consecutive patients who restarted infliximab after a median 15-month hiatus, the absence of ATI at an early sample after re-exposure to infliximab (typically before second infusion) was associated with improved short-term responses. Similarly, higher trough concentrations at an early sample after re-exposure to infliximab were associated with long-term response. Of note, ATI at an early sample after re-exposure to infliximab were associated with a higher rate of infusion reactions (with detectable ATI) after reinitiating therapy. In fact, the greatest risk of a serious acute infusion reaction is the second or third dose after a drug holiday (65). Although data are limited, testing for ADA after the first reinduction and administering the second dose only after confirmation of absence of ADA for safety reasons is recommended. The same study showed that IMM cotreatment at restart was the only clinical predictor for preventing infusion reactions, implying that an addition of an IMM when reinitiating infliximab after a drug holiday is a valid option (65).
Although commercially available assays typically correlate well, absolute drug concentrations can differ among assays or even the same type of assay (66–77). This is very important because clinical decisions are typically based on drug concentration thresholds to target. Two recent studies comparing a commercially available homogeneous mobility shift assay (HMSA) and the enzyme-linked immunosorbent assay (ELISA) to assess infliximab, adalimumab, and ustekinumab concentrations demonstrated quantitative and qualitative discrepancies in drug concentrations (72,73). Similar discrepancies have been identified between ELISAs and point-of-care assays (74). As the clinical impact of these differences has not been extensively investigated, and until commercial assays are accurately cross-validated and standardized, patients should ideally be followed and managed over time with the same TDM assay. To facilitate harmonization of TDM assays and quality control, implementation of an international standard and use of universal calibrators should be considered.
Supportive text for statement 22 is provided in Supplementary Digital Content 2 (http://links.lww.com/AJG/C133) (78–80).
Statements that reached consensus regarding immunogenicity are presented in Table 4. Supportive text for these statements is provided below. Among all statements, only 1 did not reach consensus: “Low-titer antibodies to adalimumab (ATA) can be defined as <4 μg/mL for the ELISA” (10% vote of ≥7).
Numerous studies have shown that ADA are associated with subtherapeutic or undetectable drug concentrations and undesirable clinical outcomes, such as PNR, SLR, and infusion reactions (45,57–59,64,81–88). These refer mostly to high-titer, neutralizing, persistent ADA that cannot be overcome with dose optimization and are associated with undetectable drug concentrations and treatment failure. It seems that ADA present when drug is still detectable by a drug-tolerant assay may not be clinically relevant (77,86). However, there are some data suggesting that ADA, even at low concentrations and in the presence of drug, may still be a risk factor for SLR to infliximab or adalimumab and treatment discontinuation, highlighting the importance of systematic TDM to look for an increase of ADA titers and/or undetectable drug concentrations and to determine if ADA can be overcome after treatment optimization (88,89). A recent prospective study showed that the prevalence of ATI and ATA is high when detected early using a drug-tolerant assay and their presence predicts further treatment discontinuation (89). Time to treatment discontinuation was significantly shorter in patients with ATA ≥2.0 μg/mL or ATI ≥4.0 μg/mL at week 2 compared with patients without positive ADA. In multivariate analysis, ATI or ATA at week 2 were the only factors associated with treatment discontinuation (89).
In case of SLR to anti-TNF therapy because of the development of high-titer ADA, physicians should switch to a different biologic. A study more than 10 years ago showed that in patients with detectable ATI, a change to another anti-TNF agent was associated with a complete or partial response in 92% of patients, whereas dose escalation resulted in a response of only 17% (90). More recently, Yanai et al. (7) showed that ATA >4 μg/mL or ATI >9 μg/mL identified patients who did not respond to an increased drug dosage. Although dosage increases were more effective for patients with no or low-titer ADA, patients with high-titer ADA had longer durations of response when anti-TNFs were switched than when dosage was increased.
When considering a switching within drug class, the recommendation would be to add an IMM to a subsequent anti-TNF therapy to prevent the formation of ADA to the second anti-TNF. In a recent RCT, consecutive patients with IBD who developed an SLR to monotherapy with an anti-TNF because of ADA received a second anti-TNF and were randomized to receive either combination therapy with a second anti-TNF (adalimumab, 40; infliximab, 50 patients) with azathioprine (n = 45) or a second anti-TNF as monotherapy (n = 45). Rates of clinical failure and appearance of undetectable trough concentrations with high ADA were higher in monotherapy compared with combination therapy (91).
However, the distinction between low and high ADA titers may be difficult because they are assay-specific and there are still limited data for assays other than the HMSA and for biologics other than infliximab (92). This is of great clinical importance because low-titer ADA can be overcome by treatment optimization (dose escalation, dose interval shortening, and/or addition of an IMM) (93–98), while high-titer ADA can lead to undetectable or low drug concentrations, infusion reactions, and treatment failure (45,57–59,64,81–88). Two studies showed that ATI >9.1 U/mL were associated with failure of dose intensification after SLR, infliximab discontinuation, and infusion reactions (24,85). Ben-Horin et al. (94) reported that in 5 patients with IBD and SLR to infliximab because of immunogenicity, ATI gradually decreased, drug concentrations increased, and clinical responses were restored after the administration of IMM. Ungar et al. (95) showed that in almost half of patients with IBD and SLR because of immunogenicity, ATA could be gradually reversed by the addition of an IMM with restoration of a clinical response (95). Moreover, Strik et al. (93) showed that in 77% of IBD patients with SLR because of immunogenicity, addition of IMM resulted in undetectable ADA levels, increased serum drug concentrations, and regaining of clinical response in patients treated with infliximab and adalimumab (93). Regarding ADA titers that can be overcome with treatment optimization, Papamichael et al. (96) demonstrated that ATI <8.8 U/mL using the HMSA were associated with drug retention in patients with IBD in whom infliximab was optimized, either proactively or reactively, to overcome immunogenicity. Similarly, a recent study using a large database-derived cohort showed that ATI ≤8.55 U/mL through HMSA were associated with overcoming ATI with dose escalation (98).
The formation of ADA cannot only be overcome, but also be prevented by the use of IMM (64,99–101). A retrospective multicenter study showed that thiopurine-infliximab combination therapy in patients with CD was associated with reduced ATI formation compared with infliximab monotherapy (99). In the prospective PANTS study, combination IMM (thiopurine or methotrexate) therapy mitigated the risk of developing ATI [hazard ratio: 0.39; 95% confidence interval (CI): 0.32–0.46, P < 0.001) and ATA (hazard ratio: 0.44; 95% CI: 0.31–0.64; P < 0.001) (64). A meta-analysis of 35 studies showed that combined treatment with IMM is associated with reduced risk of formation of antibodies against anti-TNF in patients with IBD. The pooled risk ratio for formation of ADA in patients receiving combined therapy with IMM vs that of patients receiving anti-TNF monotherapy was 0.49 (95% CI: 0.41–0.59; P < 0.001) (101). Finally, it seems that even lower doses (<1 mg/kg) of azathioprine can prevent immunogenicity of infliximab in patients with IBD receiving combination therapy (100).
Regarding risk factors for ADA formation, a genomewide association study found that the HLA-DQA1*05 allele increased the risk of ATI and ATA development by 2-fold in patients with CD, regardless of concomitant IMM use. The highest rates of immunogenicity, 92% at 1 year, were observed in patients treated with infliximab monotherapy who carried HLA-DQA1*05. Conversely, the lowest rates of immunogenicity, 10% at 1 year, were observed in patients treated with adalimumab combination therapy who did not carry HLA-DQA1*05 (55). In the same line, HLADQA1*05 was found to be independently associated with a high risk of ATI in addition to infliximab SLR and treatment discontinuation (56).
Immunogenicity to biologics other than anti-TNF therapy is less common. The development of ADA is relatively low for vedolizumab and ustekinumab ranging from 1% to 4.1% and 0.7%–4.6%, respectively (102,103).
Biologic drug concentrations to target
Statements that reached consensus regarding drug concentrations to target are presented in Tables 5 and Table 1, Supplementary Digital Content 1 (http://links.lww.com/AJG/C132). Supportive text for these statements is provided below and in Supplementary Digital Content 2 (http://links.lww.com/AJG/C133) (for statements 40–48).
Numerous exposure-response relationship studies suggest that biologic drug concentration thresholds and ranges seem to differ depending on treatment goals (Table 6), disease phenotypes, and assays used (2–5,31–33,39–41,81,104–118). In general, higher drug concentrations tend to be associated with more stringent outcomes such as endoscopic and histologic remission (2–5), while even higher drug concentrations may be needed for IBD phenotypes characterized by a higher inflammatory burden, such as fistulizing CD (119,120) and acute severe ulcerative colitis (46). However, these data mostly refer to infliximab and adalimumab.
We would like to highlight that for all statements regarding the biologic drug concentrations to target the suggested range was based on previously published association data (3–5) and the upper limit of range typically refers to drug concentration associated with more stringent therapeutic outcomes such as biochemical, endoscopic, histologic, or composite remission defined as any combination of the previous.
During the online meeting, it was highlighted that there are only limited data about the drug concentrations to target for certolizumab pegol, golimumab, vedolizumab, and ustekinumab (statements 40–48) and the panelists felt that robust recommendations could not be made based on so few studies and that the data be presented only as a supplementary table (see Table 1, Supplementary Digital Content 1, http://links.lww.com/AJG/C132). For these biologics, the suggested range was based on data from post hoc analysis of RCTs and prospective studies, where available (3–5).
Although most gastroenterology societies, as well as expert groups, endorse the use of reactive TDM of anti-TNF therapy, there is still a debate regarding the role of proactive TDM (10). There is also debate regarding the application of reactive TDM for non-TNF biologics and threshold drug concentrations to target.
The panel agreed that reactive TDM should be used for all biologics for both PNR and SLR. It was also recommended that treatment discontinuation should not be considered for infliximab or adalimumab until a drug concentration of at least 10–15 μg/mL is achieved. In the absence of high-quality data and reflecting also the clinical practice of the panelists, the suggested range of 10–15 μg/mL was selected based on data from incremental gain (116) and quartile analysis (107,110,120) of association studies showing that drug concentrations in quartiles (Q)3 and 4 are associated with better therapeutic outcomes. For example, infliximab concentrations ≥ 12.3 μg/mL (Q4) are associated with higher rates of endoscopic and histologic healing (110). Moreover, infliximab concentrations in Q3 (10.1–20.1 μg/mL) or Q4 (≥20.2 μg/mL) are associated with higher rates of mucosal or fistula healing as well as fistula closure (120). By using a rather higher (10–15 μg/mL) than the standard infliximab (5–10 μg/mL) or adalimumab (8–12 μg/mL) concentration range to target (mostly referring to proactive TDM), we wanted to highlight that the drug should not be inappropriately abandoned for a presumed mechanistic failure when reactive TDM is applied for SLR. This is very important because most of the SLR is attributed to pharmacokinetic (PK) issues because of low/subtherapeutic drug concentrations (2).
RCTs to test proactive TDM are more limited demonstrating inconsistent results probably also because of differences in study design and algorithms used for dose optimization (15,16,121–123). The TAXIT (15) and the TAILORIX (121) (A Study investigating Tailored Treatment With Infliximab for Active Crohn's Disease) RCTs did not reach their primary outcomes, while the PAILOT (16) and the PRECISION (123) (Precision Dosing of Infliximab vs Conventional Dosing of Infliximab) RCTs showed that proactive TDM is associated with better therapeutic outcomes compared with standard of care. More recently, the NOR-DRUM (NORwegian DRUg Monitoring study) RCT was the first study to compare the efficacy and safety of proactive TDM starting early during the induction phase with standard infliximab therapy in patients with immune-mediated inflammatory diseases, such as rheumatoid arthritis, spondyloarthritis, psoriatic arthritis, IBD, or psoriasis (122). Although the primary end point of clinical remission at week 30 and numerous secondary outcomes were not met, it is difficult to draw firm conclusions for IBD because the trial did not have the statistical power to test hypotheses within each disease subgroup. We would like to highlight that only one-third of the study population who received the randomized intervention had IBD, mucosal healing as a stringent objective therapeutic outcome was not investigated, and the 3-μg/mL infliximab concentration threshold for allowing treatment optimization seems very low based on recent data in IBD (3–5).
The panel recommended performing proactive TDM for anti-TNF after induction and at least once in the maintenance phase of therapy. It was felt that more data were needed to support the use of proactive TDM for other biologics. Moreover, the panel agreed that proactive TDM can efficiently guide therapeutic decisions in other clinical scenarios including treatment de-escalation, optimized anti-TNF monotherapy instead of combination therapy, verification of therapeutic drug concentrations after reactive testing, and assessment of ADA after restarting IFX after a drug holiday. The panel also suggested a range rather than a specific threshold of clinically relevant biologic drug concentrations to target because these can vary based on the therapeutic outcome of interest, typically being higher for more stringent outcomes such as endoscopic and histologic remission or fistula healing. Biologic drug concentrations to target may also differ based on the assay used and the IBD phenotype (2–5).
Nevertheless, additional data from prospective studies and RCTs concerning the use of proactive TDM, particularly during the induction phase, incorporating point-of-care assays (58) and/or PK dashboards (123,124) are warranted. Point-of-care assays will provide a rapid assessment of drug concentrations and allow for an immediate adjustment of drug dosage. PK dashboards integrate individual clinical and PK data to forecast dosing recommendations to target prespecified drug concentrations for individual patients and allow for more personalized care. PK modeling and pharmacogenetics to identify patients with a high risk of accelerated drug clearance and a genetic predisposition of ADA formation (55,56), respectively, would allow a selection of those patients who would benefit more from proactive TDM. Another important area that needs further investigation is the role of TDM in biologics other than infliximab and adalimumab because recommendations for these drugs are only based on exposure-outcome association studies which are limited. Finally, there is a gap in knowledge regarding the measurement of peak drug concentrations (39) and total drug exposure (125).
In conclusion, TDM of biologics is a useful tool in optimizing the care of patients with IBD. We hope that these consensus statements based on interpretation of the available literature can assist physicians in improving the care of patients with IBD.
CONFLICTS OF INTERESTS
Guarantor of the article: Adam S. Cheifetz, MD.
Specific author contributions: K.P., A.S.C: panelist, study design, data collection, analysis and interpretation, and manuscript writing; G.Y.M.: panel moderator and manuscript critical revision; M.T.A., W.A., R.K.C., M.C.D., E.V.L., M.T.O., A.S., C.A.S., and A.J.Y.: panelist and manuscript critical revision. A.S.: online meeting organizer and manuscript critical revision. All the authors reviewed and approved the final version of the manuscript.
Financial support: K.P. is supported by the Ruth L. Kirschstein NRSA Institutional Research Training Grant T32 DK007760.
Potential competing interests: K.P. reports lecture fees from Mitsubishi Tanabe Pharma and Physicians Education Resource LLC; consultancy fee from Prometheus Laboratories Inc; and scientific advisory board fees from ProciseDx Inc and Scipher Medicine Corporation. A.S.C: received consultancy fees from AbbVie, Janssen, Takeda, Bacainn, Arena Pharmaceuticals, Grifols, Prometheus, Samsung, Bristol Myers Squibb, and Pfizer and research support from Inform Diagnostics. A.J.Y. received consultancy fees from Takeda, Prometheus Bioscience, and Arena Pharmaceuticals. R.K.C. received consultancy fees from AbbVie, LabCorp, Bristol Myers Squibb, Janssen, Pfizer, Prometheus, Samsung Bioepis, and Takeda. M.T.O. has received consultancy fees for AbbVie, Bristol Myers Squibb, Elan, Genentech/Roche, Janssen, Lycera, Merck, Pfizer, Takeda, and UCB and research grant support from UCB. M.C.D. received consulting fee: AbbVie, Arena, Boehringer Ingelheim, Bristol Myers Squibb, Celgene, Eli Lilly, Genentech, Janssen, Pfizer, Prometheus Biosciences, Target RWE, Takeda, and UCB. W.A.: received consultancy fees from AbbVie, Amgen, Arena Pharmaceuticals, Dynacare, Janssen, Merck, Novartis, Pfizer, Sandoz, and Takeda. C.A.S. has received consultancy fees from AbbVie, BMS, Lilly, Janssen, Pfizer, Prometheus, and Takeda, speaker fees for CME activities from AbbVie, Janssen, Pfizer, and Takeda, grant support from AbbVie, Pfizer, and Takeda, and has an equity interest as a cofounder of Mitest Health, LLC. G.Y.M.: received consultancy fees from Boehringer-Ingelheim, Bristol Meyers Squibb/Celgene, Entasis, Genentech, Janssen, Pfizer, Samsung Bioepis, Takeda, and Techlab and research support from Pfizer. MTA has received consultancy fees from Janssen, Prometheus Bioscience, Takeda, Focus Medical Communications, Pfizer, Boehringer Ingelheim Pharmaceuticals, Gilead, Imedex, Cornerstone Health, Inc, Landos Biophama, UCB Biopharma SRL, Eli Lilly, and Cosmo Biopharma and grant support from Prometheus Bioscience, Takeda, and Pfizer; E.V.L. received consulting fees from AbbVie, Allergan, Amgen, Boehringer Ingelheim, Bristol Myers Squibb, Celgene, Celltrion, Eli Lilly, Genentech, Gilead, Iterative Scopes, Janssen, Pfizer, Takeda, and Ono Pharma, research support from AbbVie, Bristol Myers Squibb, Celgene, Genentech, Gilead, Janssen, Pfizer, Robarts Clinical Trials, Takeda, and Theravance, and he is a shareholder in Exact Sciences. He is also the Chief Medical Editor of Healio Gastroenterology and Liver Disease. The remaining authors have no conflicts of interest.
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