Secondary Logo

Journal Logo

Institutional members access full text with Ovid®

Quantitative Detection of Promoter Hypermethylation in Multiple Genes in the Serum of Patients with Colorectal Cancer

Leung, Wai K., M.D.1; To, Ka-Fai, M.D.2; Man, Ellen P.S., M.D., Ph.D.1; Chan, Michael W.Y., Ph.D.1; Bai, Alfa H.C., M.Phil.1; Hui, Aric J., M.B.Ch.B., M.R.C.P.1; Chan, Francis K.L., M.D.1; Sung, Joseph J.Y., M.D., Ph.D.1

American Journal of Gastroenterology: October 2005 - Volume 100 - Issue 10 - p 2274–2279

OBJECTIVES While promoter hypermethylation is a common molecular alteration of human colorectal cancer that could be detected in the bloodstream, we tested the feasibility of quantitative detection of aberrant DNA methylation in multiple genes in the serum samples of colorectal cancer patients.

METHODS The pre-therapeutic serum samples of 49 colorectal cancer patients and 41 age-matched controls with normal colonoscopy were examined. The presence of methylated DNA in APC (adenomatous polyposis coli), hMLH1 (human MutL homolog 1), and HLTF (helicase-like transcription factor) was detected by quantitative methylation-specific PCR (MethyLight).

RESULTS There was a significant difference in the concentration of methylated serum DNA between cancer patients and controls for HLTF (p = 0.015) and hMLH1 (p = 0.0001) genes, but not for APC gene (p = 0.21). In total, 28 patients with colorectal cancer and 4 controls had methylated DNA detected in at least one marker, which gave a sensitivity of 57% and specificity of 90%. All patients with methylation in two methylation markers had advanced (stage III/IV) cancer (p = 0.006) and patients with methylation in at least one marker tended to have a lower probability of survival (p = 0.08).

CONCLUSION The quantitative detection of aberrant DNA methylation in serum may be a promising high-throughput approach for the noninvasive screening and monitoring of colorectal cancer.

1Department of Medicine & Therapeutics, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong

2Department of Anatomical & Cellular Pathology, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, Hong Kong

Reprint requests and correspondence: Wai K. Leung, Department of Medicine & Therapeutics, Prince of Wales Hospital, 30–32 Ngan Shing Street, Shatin, Hong Kong.

Received March 16, 2005; accepted April 22, 2005.

© The American College of Gastroenterology 2005. All Rights Reserved.
You currently do not have access to this article

To access this article:

Note: If your society membership provides full-access, you may need to login on your society website