T-cell expression of PD-1, PD-L1 and TIM-3 in HIV-2 infection.(a) PD-1 mean fluorescence intensity (MFI) assessed within total CD8 and CD4 T-cells in seronegative, HIV-1 and HIV-2 cohorts. (b) Correlations between PD-1 and PD-L1 MFI within total CD8 and CD4 T-cells in the three cohorts. (c) Illustrative dot-plots of PD-1 and TIM-3 expression within naive and memory CD8 T-cell subsets, defined according to CD45RA and CD27, in an HIV-1-positive patient with 650 CD4 T-cells/μl and 21 731 RNA copies/ml. (d) TIM-3 MFI within total CD8 and CD4 T-cells in seronegative, HIV-1 and HIV-2 cohorts in early-stage disease as described in Supplementary Digital Content, Table 3. (e) Dot-plots illustrate the analysis of TIM-3 and PD-1 in relation to Ki-67 within total CD8 T-cells in the same patients as in (c). The relative contribution of TIM-3+ and PD-1+ cells to the pool of cycling CD8 and CD4 T-cells (Ki-67+) are shown in the graphs. (f) Correlations between the expression of the pro-survival factor Bcl-2 and TIM-3 or PD-1 expression within CD8 and CD4 T-cells in the three cohorts. Spearman correlation coefficients are shown. Each dot represents one individual. Bars represent mean.