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IL-27 posttranslationally regulates Y-box binding protein-1 to inhibit HIV-1 replication in human CD4+ T cells

Poudyal, Deepaka; Yang, Juna; Chen, Qiana; Goswami, Suranjanaa; Adelsberger, Joseph W.b; Das, Sudiptoc; Herman, Andrewa; Hornung, Ronald L.d; Andresson, Thorkellc; Imamichi, Tomozumia

doi: 10.1097/QAD.0000000000002288
BASIC SCIENCE
Open
SDC

Objectives: IL-27 is known as an antiviral cytokine that inhibits HIV, hepatitis C virus, and other viruses. We have previously demonstrated that, IL-27 posttreatment after HIV-infection inhibits viral replication in primary CD4+ T cells.

Design: Here, we evaluated the anti-HIV effect of IL-27 pretreatment in CD4+ T cells from healthy donors prior to HIV infection with HIVNL4.3 or vesicular stomatitis virus G glycoprotein (VSV-G)-pseudotyped HIV-luciferase virus (HIV-LUC-V).

Methods: IL-27-treated CD4+ T cells were infected with HIVNL4.3 or HIV-LUC-V and assessed the anti-HIV effect. HIV infection was monitored by p24 antigen ELISA or luciferase assay. HIV fusion/entry and uncoating were determined by BlaM-Vpr assay and HIV fate of capsid and/or HIV Entry/Uncoating assay based on core-packaged RNA availability and Translation assay, respectively. HIV proviral copy number was determined by real-time PCR. Gene expression profile from IL-27-pretreated CD4+ T cells was determined using Genechip array. Posttranslational modification of global proteins from IL-27-pretreated CD4+ T cells was determined by a combination of 2-dimensional difference-in-gel-electrophoresis (2D-DIG), western-blot and protein mass spectrometry.

Results: IL-27 pretreatment inhibited HIVNL4.3 and HIV-LUC-V infection in CD4+ T cells. HIV copy assay demonstrated that IL-27-treatment suppressed an early step of reverse transcription during HIV infection. A combination of 2D-DIG-electrophoresis and western blot assays demonstrated that IL-27-treatment induces a change in posttranslational modification of Y box binding protein-1 (YB-1). Overexpression of domain negative YB-1 mutants illustrated that a residue Lysine at 118 plays a key role in supporting HIV infection in CD4+ T cells.

Conclusion: IL-27-pretreatment inhibits HIV-1 infection by suppressing an HIV-reverse transcription product formation/uncoating step by suppressing the acetylation of YB-1 in primary CD4+ T cells.

aLaboratory of Human Retrovirology and Immunoinformatics

bAIDS Monitoring Laboratory

cProtein Characterization Laboratory

dImmmunological Monitoring Laboratory, Applied and Developmental Research Directorate, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, USA.

Correspondence to Tomozumi Imamichi, Building 550, Room 126, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, MD 21702, USA. E-mail: timamichi@mail.nih.gov

Received 25 January, 2019

Revised 2 June, 2019

Accepted 7 June, 2019

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Introduction

We have previously reported that the pleotropic cytokine IL-27 inhibits HIV replication in primary CD4+ T cells, monocyte-derived macrophages, and dendritic cells [1–4]. IL-27 is a heterodimeric cytokine composed of Epstein–Barr virus-induced gene 3 and IL-27p28 that belongs to the IL-6/IL-12 family of cytokines [5]. It is primarily secreted by macrophages and dendritic cells [6–8]. A recently published report indicated that plasma IL-27 levels in HIV patients were not significantly altered during HIV-1 infection [9], suggesting that IL-27 could potentially be used in anti-HIV therapy [10].

HIV utilizes host proteins to complete every step of its life cycle and replication process [11–13]. In addition, the host cells are equipped with proteins known as ‘host restriction factors’ that counter the HIV replication process. Cell survival and function are critically dependent on numerous cellular signals that are mostly mediated by posttranslational modifications (PTMs) of the proteins. Several studies report that PTMs of the host proteins either enhance or inhibit HIV replication [14–17], it is reported that Y-box binding protein-1 (YB-1), a DNA-binding and RNA-binding protein [18,19], serves as a host protein that stabilizes the genomic RNA of HIV-1 to promote HIV production in 293T cells [20]. It is also involved in HIV replication in Jurkat T cells [21]. In the current study, we investigated the anti-HIV mechanism of IL-27 in primary T cells. We now report, for the first time, that acetylated YB-1 is a key factor for HIV infection in primary T cells and that IL-27 regulates acetylation followed by HIV infection in T cells.

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Material and methods

Participants were informed written consent prior to blood being drawn. All experimental procedures in these studies were approved by the National Cancer Institute at Frederick and National Institute of Allergy and Infectious Diseases (NIAID) Institutional Review Board and performed in accordance with the relevant guidelines and regulations.

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Plasmids

pNL4.3 [22] and pNL4.3.Luc [23,24] were obtained from the AIDS Reagents Program of the NIAID/National Institutes of Health (NIH) YB-1 expression vectors were constructed as follows: YB-1 wild-type and YB-1 lysine-mutated genes were synthesized (Eurofins MWG Operon, Louisville Kentucky, USA) and cloned into pCMV5.1-Flag to obtain plasmid wild-type-YB-1-Flag and Mut-YB-1-Flag.

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Cells and viruses

CD4+ T cells were isolated from peripheral blood mononuclear cells of healthy donors using CD4+ microbeads according to the manufacturer's instructions (Miltenyi Biotec, San Diego, California USA), as previously described [1]. Isolated CD4+ T cells were stimulated with phytohemagglutinin (PHA) (Sigma-Aldrich, St. Louis, Missouri, USA) at 5 μg/ml for 3 days in RPMI-1640 media supplemented with 10% fetal bovine serum (FBS, Hyclone; Thermo Fisher Scientific, Waltham, Massachusetts, USA), 10 mmol/l HEPES: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (Quality Biological, Gaithersburg, Maryland, USA), and 5 μg/ml of gentamycin (Thermo Fisher Scientific) (RP-10) [1]. 293T cells were obtained from the American Type Culture Collection and maintained in D-MEM (Thermo Fisher Scientific) containing 10% FBS. HIV-1 stocks were prepared using plasmids encoding the full length of HIVNL4.3 [22] as previously mentioned [2,25]. VSV-G-pseudotyped HIV-luciferase virus (HIV-LUC-V) virus was prepared as previously mentioned [3]. HIV-1 virions containing BlaM-Vpr were produced by cotransfection of 293T cells (1.5 × 106 cells) with pNL4–3 proviral DNA (6 μg), pCMV-BlaM-Vpr (2 μg), and pAdVAntage vectors (1 μg) as described [26]. HIV Entry/Uncoating assay based on core-packaged RNA availability and Translation (EURT) virus was produced as previously described [27].

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Pretreatment of CD4+ T cells with IL-27 and panabinostat

The PHA-stimulated CD4+ T cells were pretreated with IL-27 prior to HIV infection. Briefly, the cells were cultured with either 20 U/ml of recombinant IL-2 (Roche, Mannheim, Germany) alone (control cells) or IL-2 with 100 ng/ml of recombinant IL-27 (R&D Systems, Minneapolis, Minnesota, USA) (treated cells) for 3 days in RP-10 at 2 × 106 cells/ml. After incubation, the cells were washed with prewarmed RP-10 medium at 500 × g for 5 min for three times, and then maintained in RP-10 with 20 U/ml of IL-2. Panabinostat (Selleckche, Houston, Texas, USA) treatment was performed as follows: PHA-stimulated CD4+ T cells were pretreated with 35 nmol/l Panabinostat for 24 h. Following the pretreatment, CD4+ T cells were treated with IL-2 or IL-2 with IL-27 for 3 days and then cells were washed as described above.

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Flow cytometry analysis

Expression of CD4+, CXCR4, and CD3 on CD4+ T cells was analyzed by flow cytometry. Cells were stained for 15 min at room temperature in PBS containing 2% bovine serum albumin with the following antibodies: Pacific Blue-conjugated anti-CD3 (Becton Dickinson, Franklin Lakes, New Jersey, USA), APC-Cy7-conjugated anti-CD4+ (Becton Dickinson), and phycoerythrin (PE)-conjugated anti-CXCR4 (Becton Dickinson), all of which were performed following the vendor's protocol (Abcam, Cambridge, Massachusetts, USA). The cell preparations were analyzed with a FACSCalibur flow cytometer (Becton Dickinson). Positive and negative events were determined with matching isotype controls.

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HIV-1 replication assay and HIV single-round infection

IL-2 or IL-2/IL27-pretreated CD4+ T cells were infected with HIVNL4.3 as previously described [2]. Half of the culture supernatants were replaced with fresh medium on day 3. Viral replication was gauged from p24 levels in culture supernatants using an HIV-1 p24 ELISA kit (PerkinElmer, Waltham, Massachusetts, USA). For single-round infection, CD4+ T cells were incubated with HIV-LUC-V (100 ng/ml p24) for 2 h. Cells were washed and then cultured for 2 days in the presence of IL-2. Luciferase activity was measured with a Bright-Glo Luciferase Assay System (Promega, Madison, Wisconsin, USA).

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HIV binding assay

CD4+ T cells were incubated with virus at 4 °C for 80 min to allow binding but not membrane fusion, as previously described [28], and then washed to remove unbound virus. Cells were lysed using RIPA buffer (Boston BioProducts, Ashland, Massachusetts, USA) to obtain the protein, which was followed by a HIV-1 p24 ELISA to assess the level of bound HIV.

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Quantitative reverse transcription-PCR

Cells were washed using cold PBS, and RNA was isolated from the cells using an RNeasy Isolation kit (Qiagen, Germantown, Maryland, USA). Total complementary DNA (cDNA) was synthesized using TaqMan reverse transcription reagents (Applied Biosystems, Foster City, California, USA). YB-1 and other gene expression levels were measured using quantitative real-time polymerase chain reaction (qRT-PCR) on a CFX96 Real-Time system (Bio-Rad Laboratories, Hercules, California, USA) and normalized by glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Probes information is as follows: YB-1/YBX-1 (Hs00358903_g1), SUB1 (Hs00743451_s1), tryptophanyl-tRNA synthetase (WARS) (Hs00188259_m1), HnRNPH1 (Hs03044422_g1), CALM1 (Hs00300085_s1), MYL12B (Hs00853081_g1), PDCD6 (Hs00918238_m1), TPI1 (Hs03806547_s1), PGAM1 (Hs01652468_g1), PPIA (Hs04194521_s1), IMMT (Hs00986184_mH), LAP3 (Hs00429769_m1), CAPG (Hs00156249_m1), ACTG1 (Hs03044422_g1), STAT1 (Hs01014002_m1), MX1 (Hs00182073-ml), and GAPDH (Hs99999905_m1) (Applied Biosystems).

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HIV reverse transcription product assay

Control and IL-27-pretreated CD4+ T cells were infected with 100 ng/ml of pseudotyped HIV (DNase I-pretreated) for 2 h. Twelve hours postinfection, genomic DNA was isolated using a QIAamp DNA Blood Mini Kit (Qiagen). A total of 500 ng of genomic DNA was used for each reaction of qRT-PCR. Absolute copy number per 1 × 106 cells were normalized by RNase P housekeeping gene (Applied Biosystems), as previously described [3].

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SDS–PAGE and western blot analysis

CD4+ T cells were washed with ice-cold PBS and resuspended in RIPA buffer with a protease inhibitor cocktail (Sigma-Aldrich) and phosphatase inhibitors (Thermo Fisher Scientific) at 1 × 106 cells/100 μl at 4 °C for 10 min. The protein concentration was determined using a bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific). Using a total of 20-μg protein, western blot analysis was performed as previously described [29], and the intensity of the band was analyzed using NIH ImageJ (http://rsbweb.nih.gov/ij/). The western blot membrane was stripped (Restore western Blot Stripping Buffer, Thermo Fisher Scientific) and reprobed with another antibody or β-actin (Sigma-Aldrich) wherever necessary. The antibody information is as follows: PDCD6 (Proteintech, Rosemont, Illinois, USA), (Mitofilin, IMMT, WARS, Cyclophilin, HnRNPH1, MYL12B, PC4, TPI1, CALM1, LAP3, CAPG, PGAM, YBX-1/YB-1) (Abcam), β-actin (Sigma-Aldrich), STAT1 and phospho-S102-YB-1 (Cell Signaling Technology, Danvers, Massachusetts, USA).

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2-Dimensional difference-in-gel-electrophoresis, two-dimensional gel electrophoresis, and western blot analysis

Whole-cell lysate protein samples from control and treated CD4+ T cells were supplied to Applied Biomics (Hayward, California, USA) for identification of regulated global proteins by 2-dimensional difference-in-gel-electrophoresis (2D-DIGE).

Whole-cell lysate proteins (100 μg) were subjected to 2D-PAGE analysis as described [30]. After separation by 2D-PAGE, the samples were transferred to a membrane for western blot analysis.

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Transfection of small interfering RNA (siRNA) or DNA into CD4+ T cells

YB-1-specific siRNA (SR303243-C) and scrambled control siRNA (SR30004) were purchased from OriGene (Rockville, Maryland, USA). CD4+ T cells (7 × 106/cuvette) were transfected with siRNA (300 nmol/l) or plasmid DNA (2 μg) using a P3 Primary Cell 4D-Nucleofector Kit (V4XP-3024) using E0–115 pulse program code (Lonza, Basel, Switzerland) followed by warm RP-10 medium incubation overnight. The cells were washed and stimulated with PHA/IL-2 for 3 days. siRNA/DNA nucleofection efficiency was assessed by reverse transcription-PCR and western blot after 3 days of stimulation.

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BlaM-Vpr assay for fusion and entry

The BlaM-Vpr entry/fusion assay was performed as previously described [26], and the fusion and entry activity in the samples were analyzed by flow cytometry using excitation at 410 nm and emission at 450/550 and 525/550 nm.

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Immunoprecipitation and identification of posttranslational modification of Y box binding protein-1

Following nucleofection of CD4+ T cells with 2 μg of wild-type-YB-1-Flag plasmid, cells were stimulated with PHA, followed by IL-2 or IL-2/IL-27 treatment for 3 days. Cells were harvested and lysed in IP lysis buffer (Thermo Fisher Scientific). A total of 500 μg of protein was used for immunoprecipitation, following the manufacturer's protocol (Sigma-Aldrich) using glycine/Tris elution. The eluted protein was concentrated using methanol/chloroform precipitation, and samples were subjected to mass spectrometry (MC) analysis for identification of PTM.

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Fate-of-capsid assay

The fate-of-capsid assay was performed as previously described [31], with 10 × 106 control or IL-27-treated CD4+ T cells infected with pseudotyped HIV-LUC-V virus (250 ng/ml) for 2 h, then washed and incubated for 12 h in RP-10 medium with IL-2. Infected CD4+ T cells were harvested and resuspended in 2.5 ml of hypotonic lysis buffer (10 mmol/l Tris–Cl pH 8.0, 10 mmol/l KCl, 1 mmol/l ethylenediaminetetraacetic acid (EDTA), complete protease inhibitors (Roche) and a phosphatase inhibitor). After ultracentrifugation as previously described [31], the top layer above the sucrose cushion was collected as the ‘soluble’ fraction (1 ml), and, once the remaining sucrose was removed, SDS–PAGE loading dye was added to the tube to resuspend the ‘pellet’ fraction. Fractions were analyzed for capsid protein content by immunoblotting using a mouse monoclonal anti-HIV-1 p24 antibody (Abcam).

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HIV Entry/Uncoating assay based on core-packaged RNA availability and translation assay

The HIV EURT assay for HIV core protein uncoating was performed as previously described [27]. Briefly, control and treated CD4+ T cells were infected with 500 ng/ml p24 HIV EURT virus for 2 h. Following infection, the cells were incubated in RP-10 medium with IL-2 for 12 h. Luciferase activity was measured with a Bright-Glo Luciferase Assay System (Promega).

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Statistical analysis

Statistical analyses were performed using GraphPad Prism 5 software (San Diego, California, USA). Error bars indicate SDs or standard errors (SE) from means as noted. An unpaired Student's t test was used, and P values lower than 0.05 were considered significant.

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Results

Pretreatment with IL-27 confers CD4+ T cells with resistance to HIV-1 infection

PHA-stimulated IL-27-pretreated CD4+ T cells were infected with HIV-1NL4.3 or pseudotyped HIV-LUC-V, and HIV replication was then monitored. IL-27 pretreatment inhibited p24 in culture by 66 ± 10% (P < 0.01, n = 3) (Fig. 1a) and inhibited luciferase activity in CD4+ T cells by 68 ± 4.2% (P < 0.01, n = 7) (Fig. 1b). IL-27 did not alter the expression of CD4+ and CXCR4 on CD4+ T cells (Suppl. Fig. S1a, http://links.lww.com/QAD/B493) and showed no change in cell cycle progression (Suppl. Fig. S1b, http://links.lww.com/QAD/B493), indicating that IL-27 doesn’t change the replication status or cell growth of CD4+ T cells. The supernatants of control and treated CD4+ T cells showed no significant difference in cytokine release profiles, confirming that IL-27-mediated HIV resistance may be caused by a direct effect and not by other secreted cytokines (Suppl. Fig. S1c, http://links.lww.com/QAD/B493). These observations confirm that pretreatment with IL-27 directly mediates resistance to HIV-1 infection without compromising the normal functions of CD4+ T cells.

Fig. 1

Fig. 1

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IL-27 inhibits the early steps of HIV replication in CD4+ T cells

To determine which part of the HIV life cycle is suppressed in the IL-27-treated cells, we first compared HIV-1NL4.3 binding to CD4+ T cells. The amount of viral p24 on the cell surface was not altered by IL-27 pretreatment, indicating that HIV binds at the same intensity during infection in both control and treated CD4+ T cells (Fig. 2a). Next, we assessed the impact of IL-27 on HIV internalization or fusion/entry into the host cells using the BlaM-Vpr fusion/entry assay [26]. No significant differences were observed between the control and treated cells in terms of CCF2-AM dye cleavage, an indicator of viral fusion/entry. Thus, IL-27 doesn’t affect the fusion/entry step of HIV infection (Fig. 2b), suggesting that IL-27 treatment may affect the reverse transcription step.

Fig. 2

Fig. 2

To define IL-27's role, an early reverse transcription product (R-U5) and a late reverse transcription product (U5-Gag) were semi-quantitated using qPCR. IL-27 inhibited the early reverse transcription product by 72 ± 7.0% and inhibited the late reverse transcription product by 64 ± 1.0% (n = 3) (Fig. 2c and d). In its natural state, HIV-1 provirus utilizes long terminal repeat (LTR) and Tat to mediate viral gene transcription. By cotransfecting the plasmid-encoding LTR-Luc and Tat [3], we were able to evaluate HIV promoter activity in control and IL-27-pretreated CD4+ T cells. Pretreating CD4+ T cells with IL-27 had no significant effect on viral transcription (n = 3, P = 0.15) (Fig. 2e). Thus, IL-27 pretreatment inhibits the reverse transcription step in the HIV-1 life cycle.

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IL-27 regulates the posttranslational modification

HIV-1 utilizes host cell proteins as host-required factors to facilitate and complete its replication cycle. We hypothesized that IL-27 changes the expression of host factors in CD4+ T cells. To identify the potential host factor involved in IL-27-mediated HIV-1 inhibition, we compared gene expression between control and IL-27-treated T cells. A total of 25 000 genes were analyzed (Suppl. Fig. S2a, http://links.lww.com/QAD/B493), and only Radical S-Adenosyl Methionine Domain Containing Protein-2 (RSAD2) and Interferon-Induced Protein with Tetratricopeptide Repeats 3 (IFIT3) were upregulated more than four-fold in IL-27-treated cells. None of the genes were downregulated by more than four-fold. Real-time PCR confirmed increased gene expression in IL-27-treated CD4+ T cells (Suppl. Fig. S2b, http://links.lww.com/QAD/B493). The knockdown of either RSAD2 or IFIT3 by siRNA had no effect on the HIV-LUC-V infection (Suppl. Fig. S2c and d, http://links.lww.com/QAD/B493), suggesting that IL-27 may regulate an unknown host factor by PTM.

Since PTM regulation is an essential factor in cellular responses to stimuli and infection, IL-27 may mediate HIV-1 inhibition through PTM. We utilized a 2D-DIGE approach to determine the global protein modifications (Fig. 3a). A total of 17 proteins were either downregulated or upregulated in the 2D-DIGE analysis (Suppl. Table 1, http://links.lww.com/QAD/B493). No significant differences in the total protein expression of each selected proteins were found in 1D-gel western blot (Suppl. Fig. S3a, http://links.lww.com/QAD/B493). However, a 2D-western blot analysis revealed that IL-27 treatment significantly changed in PTM of Signal Transducer and Activator of Transcription-1, Myxovirus Resistance Protein-1, Tryptophanyl-TRNA Synthetase (WARS), and YB-1 (Fig. 3b, Suppl. Fig. S3b, http://links.lww.com/QAD/B493). In 2D gel-western blot YB-1 expression from control CD4+ T cells shifted the pI toward pH 4 from a pI of pH 9.87, indicating an additional negative charge on YB-1. In the IL-27-treated CD4+ T cells, YB-1 expression shifted from pH 4 through pH 7, with another smear of band around pH 9.5 (Fig. 3b). Quantitative analysis using ImageJ (NIH) demonstrated that unmodified YB-1 in control and treated cells represented 20 and 60%, respectively, of total YB-1 (n = 4) (Suppl. Fig. S3c, http://links.lww.com/QAD/B493).

Fig. 3

Fig. 3

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Y box binding protein-1 is an essential host-required factor for HIV infection

We sought the anti-HIV effects of the four posttranslationally regulated proteins using siRNA. Of the four, only siRNA-knockdown of YB-1 expression significantly inhibited the HIV-LUC-V infection (Fig. 4a and b).

Fig. 4

Fig. 4

We further analyzed the effect of knocking down YB-1 in reverse transcription product formation following HIV-LUC-V infection. Figure 4c shows the knockdown efficiency of YB-1 siRNA in CD4+ T cells (69% inhibition of YB-1 expression). This led to downregulation of the early and late reverse transcription product by 50 and 46%, respectively, compared with negative-control siRNA-transfected CD4+ T cells (Fig. 4d and e). This result suggested that YB-1 is a host requirement factor that supports the HIV reverse transcription reaction in CD4+ T cells during HIV replication.

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Acetylated Y box binding protein-1 at lysine 118 residue is an essential host-required factor

To identify the PTM of YB-1 responsible for HIV replication, we overexpressed Flag-tagged wild-type-YB-1 in CD4+ T cells and cultured with IL-2 either alone or with IL-27. A 2D-gel western blot using an anti-Flag antibody demonstrated that overexpressed Flag-YB-1 exhibits a PTM pattern much like endogenous YB-1 (Fig. 5a and b). Therefore, we used the cell lysate of the YB-1-Flag-overexpressed CD4+ T cells for MC to determine PTM as described in the materials and methods.

Fig. 5

Fig. 5

Liquid chromatography–MC (LC/MS) revealed that lysine (K) residues K118, K137, and K264 in YB-1 were acetylated in control versus treated samples (Suppl. Fig. S4a–c, http://links.lww.com/QAD/B493) indicating that the acetylated YB-1 plays a key role in HIV reverse transcription step and IL-27-mediated anti-HIV effect is led by inhibition of the YB-1 acetylation, which may be caused by either suppression of acetylation or enhancement of deacetylation of YB-1 by IL-27. To further confirm that posttranslational regulation of YB-1 (inhibition of acetylation) acetylation is a key event in IL-27-mediated anti-HIV effect in CD4+ T cells, we pretreated the PHA-stimulated CD4+ T cells with a pan-histone deacetylase inhibitor, Panabinostat (35 nmol/l for 24 h at 37 °C), followed by IL-27 pretreatment, and infected the cells with HIV-LUC-V. Without Panabinostat treatment, IL-27 suppressed HIV replication to 47 ± 8.5% (n = 3) and the inhibitor treatment restored the HIV replication to 84.5 ± 2.7% (n = 3) (Suppl. Fig. S4d, http://links.lww.com/QAD/B493). This result illustrated that IL-27-mediated anti-HIV effect is led by induction of deacetylation of YB-1.

To identify the K residues responsible for HIV replication in YB-1, we designed YB-1 mutants with all three K residues mutated and three-mutants with a single K residue mutated to arginine (R). Following overexpression of wild-type-YB-1 and mutant-YB-1, CD4+ T cells were infected with HIV-LUC-V. If YB-1 played a key role in HIV infection in T cells, it was assumed that overexpression of YB-1 would increase HIV infection. As expected, the overexpression of YB-1-wild-type-Flag increased HIV infection (Fig. 5c). Overexpression of YB-1 mutants lacking K137 (YB-1-ΔK137R-Flag) or K264 (YB-1-ΔK264R-Flag) also increased HIV infection. In contrast, overexpression of the YB-1 mutant lacking K118 (YB-1-ΔK118R-Flag) and YB-1-Mut-Flag (all three mutations: K118R/K137R/K264R) had no impact on HIV infection (n = 3) (Fig. 5c). Overexpression of each YB-1 protein in CD4+ T cells was confirmed by western blot (Fig. 5d). Acetylation of lysine-118 residue in YB-1 is therefore key in HIV replication. When this site was mutated to disable acetylation, HIV replication was set to the basal level in CD4+ T cells.

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IL-27 inhibits the uncoating of the HIV capsid core

After infection, the HIV core proteins must be disassembled in the host cell cytoplasm to release the reverse-transcribed viral genome. Therefore, the reverse transcription process is closely tied to the shedding of the viral capsid core proteins [33]. Since IL-27 inhibits the reverse transcription process in CD4+ T cells, we further investigated the role of IL-27 during the uncoating process. If IL-27 inhibited the uncoating steps, the amount of intact core protein (stable core) in IL-27-treated cells would be higher. The fate-of-capsid assay (Fig. 5e and f, Suppl. Fig. S5a–d, http://links.lww.com/QAD/B493) revealed that 34 ± 0.4% (n = 3) and 59 ± 5.3% (n = 3) of a total p24 proteins were in intact capsid core of control and IL-27-treated cells, respectively. This indicates that the capsid core shedding is 1.8-fold (P < 0.05) lesser in IL-27-treated CD4+ T cells. In the pelleted fraction (capsid core fraction), YB-1 protein was also detected, indicating either a potential interaction between capsid p24 and YB-1 protein during the viral capsid uncoating process or that YB-1 binds to HIV genomic RNA and gets pulled down in the pelleted fraction [20].

A recently established EURT using a reporter mRNA-coding luciferase [27] was applied to the control and IL-27-treated CD4+ T cells. Then, we measured the firefly luciferase activity and observed a 35% reduction in the treated CD4+ T cells (Fig. 5g, n = 4), indicating that control CD4+ T cells exhibit optimal uncoating and IL-27 treatment inhibits the uncoating process. We have confirmed that IL-27-pretreated CD4+ T cells inhibit the uncoating process of the HIV capsid core protein, thereby inhibiting the reverse transcription product formation and, ultimately, suppressing HIV-1 replication.

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Discussion

Our study has demonstrated that IL-27 promotes PHA-activated T cells into HIV-1-resistant T cells. IL-27 blocks HIV-1 infection post entry through a PTM of YB-1 that suppresses HIV core protein uncoating and inhibits the reverse transcription process. Silencing YB-1 in T cells strongly inhibits HIV-1 infection. Conversely, overexpression of YB-1 in T cells markedly increases the susceptibility of T cells to infection. Thus, our results suggest that modulating YB-1 by PTM or expression is an effective way to render human T cells resistant to HIV-1 infection (all assays and results are summarized in Suppl. Table 2, http://links.lww.com/QAD/B493).

Several reports have indicated that capsid uncoating is required for reverse transcription complex (RTC) formation/activation [34,35], which collectively supports the idea that HIV core uncoating precedes the reverse transcription process. In our study, the PTM of YB-1 by IL-27-pretreatment profoundly delayed capsid core shedding, thereby inhibiting proper RTC activation (which is observed by the diminished reverse transcription products followed by inhibition of HIV-1 replication). This supports the finding that uncoating precedes the reverse transcription process. The capsid uncoating was only 40% lesser in IL-27-treated CD4+ T cells compared with control cells; however, the inhibition of HIV-1 replication in IL-27-treated CD4+ T cells was 65–70% (Fig. 1). This difference could be attributed to the fact that uncoating occurs early in the HIV-1 replication process, which eventually influences the reverse transcription, nuclear translocation, and integration. That has an additive effect on overall HIV-1 replication, which cumulatively amplifies the inhibitory effects. Therefore, IL-27-pretreatment inhibits HIV capsid core uncoating (Fig. 5e–g) and overall HIV-1 replication. In addition, when using HIV EURT virus, luciferase activity fell to 65% in IL-27-treated cells (n = 4) (Fig. 5g). Since we had observed low reverse transcription product (Fig. 4d and e) and inhibition of HIV-1 replication (Fig. 4b) in YB-1-silenced CD4+ T cells, we hypothesized that this leads to delayed HIV core uncoating in YB-1 k/d CD4+ T cells. In YB-1-silenced CD4+ T cells, increased accumulation of the pelleted p24 protein (1.42-fold) was observed compared with the negative siRNA control CD4+ T cells (Suppl Fig. S5e, http://links.lww.com/QAD/B493) which is an indication that in the absence of YB-1, there is suppressed or delayed uncoating of HIV p24 capsid core. This finding further supports our hypothesis that YB-1 supports HIV replication by facilitating uncoating of HIV p24 core protein and in the absence of YB-1, uncoating is delayed or suppressed in infected CD4+ T cells. To precisely define mechanism of acetylated YB-1 and role of residue of K118 on uncoating of core protein, we need further study.

HIV EURT assay detects an entered/uncoated HIV-1 that is based on the direct translation of a viral genomic RNA mimic coding a luciferase reporter that is delivered in target cells after viral-to-cellular membrane fusion in viral cores [27]. The HIV-1 entry/uncoating RNA genome coding Firefly luciferase (EU-repRNA-Luc) is added with the HIV-1 packaging sequence (Ψ) upstream of Firefly luciferase followed by a poly A signal so that it behaves as a genomic viral RNA and gets encapsidated into virion particles. Upon virion maturation and Gag processing, this genome is contained within the viral core. Once the virion is delivered via infection into target cells, it is based on the two states of the capsid core (opened/partially opened and closed). Where opened/partially opened, it allows the cellular ribosome machinery to translate luciferase protein, reflecting the uncoating process. If the core is closed, the cellular translation machinery, lacking access to EU-repRNA-Luc, will not translate luciferase protein. Thus, the luciferase assay reflects the uncoating process (the fate and status of viral core) in the HIV EURT assay. Using both the fate-of-capsid assay and HIV EURT assay [27], we have confirmed that IL-27 pretreatment inhibits the HIV capsid core uncoating process; consequently, this could be the reason for reverse transcription product inhibition and, ultimately, HIV replication inhibition.

Recent data indicate that nucleotides required for reverse transcription are channeled into the core through electrostatic channels present in the capsid hexamer [36]. Thus, in control CD4+ T cells, the dynamic capsid pores (with optimal uncoating process) may support nucleotide import and enhance the encapsidated DNA synthesis. Conversely, in treated CD4+ T cells, the delayed uncoating process may disrupt the dynamics of nucleotide import, thereby inhibiting the reverse transcription products. However, this is just speculation, and a measure of incoming nucleotides into the capsid core is necessary to provide a stronger argument.

Rankovic et al. provided a strong argument that, during the reverse transcription process, the stiffness of the core increases immensely, which mechanically triggers capsid disassembly by stressing the capsid lattice, leading to core breakage [33]. Furthermore, they supported the idea that HIV uncoating may occur only after the core encounters a cytoplasmic environment favorable for completing reverse transcription [33]. Overall, there are several theories of HIV uncoating, and it remains poorly understood.

The YB-1 protein consists of 324 amino acids composed of three domains: the N-terminal Alanine/proline-rich domain (amino acids 1–54), Cold Shock Domain (CSD) (amino acids 55–128), and C-terminal domain (amino acids 129–324) [37]. From LC/MS, we found that lysine K-118, K-137, and K-264 residues are acetylated in CD4+ T cells permissive to HIV-1 replication and that this acetylation is inhibited in IL-27-treated CD4+ T cells resistant to HIV-1 replication. K-118 is a key residue for HIV-1 replication, which shows the importance of YB-1's CSD domain. Although the role of PTM of YB-1 has been well studied in cancer research [38] PTM of YB-1 in HIV-1 replication has not been reported. Our report provides the first evidence that acetylated YB-1 supports HIV-1 replication in human primary CD4+ T cells.

In conclusion, our data indicate that YB-1 is posttranslationally regulated by IL-27 in activated CD4+ T cells to inhibit HIV-1 replication. IL-27-mediated posttranslational regulation of YB-1 has a profound effect on the early stages of HIV replication inhibition. It is known that YB-1 is a multifunctional protein that plays important roles in transcriptional and translational regulation, DNA repair, drug resistance, and stress responses to extracellular signals [39]. A recent study using T-cell lines reported that YB-1 helps support HIV replication in cell line [40], as well, indicating that YB-1 supports HIV replication not only in primary T cells but also in the cell line. Whether or not the anti-HIV effect may have clinical activity, especially for the perspective of the persistent reservoir, requires further study. However, our study provides a potent novel target of posttranslational regulation of YB-1 in CD4+ T cells for anti-HIV therapy. It is possible that IL-27 could be used as a therapeutic intervention, especially in patients infected with multidrug resistant virus.

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Acknowledgements

The authors thank Dr H. Clifford Lane for supporting and discussing this project; B. Sherman, X. Hu, and H. Sui for discussing the data and providing critical review of the article; and L. Fuentes and M. Bosche for running assays. The authors also thank Dr Andrea Cimarelli for kindly providing us the pEU-repRNA plasmid for the HIV EURT assay.

Author contributions: D.P. and T.I. designed the study. D.P., Q.C., S.G., J.W.A., A.H., S.D., and T.I. performed research and analyzed data. J.Y., R.L.H., and T.A. analyzed the data. All authors approved the final version of the article.

The project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. HHSN261200800001E. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organizations imply endorsement by the US Government. This research was supported in part by the National Institute of Allergy and Infectious Disease.

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Conflicts of interest

There are no conflicts of interest.

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Keywords:

2-dimensional difference-in-gel-electrophoresis (2D-DIG); acetylation; cytokine; IL-27; posttranslational modification; primary T cells; Y box binding protein-1

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