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EPIDEMIOLOGY AND SOCIAL

HIV stigma and viral load among African-American women receiving treatment for HIV

Kemp, Christopher G.a; Lipira, Laurenb; Huh, Davidc; Nevin, Paul E.a; Turan, Janet M.e; Simoni, Jane M.a,d; Cohn, Susan E.f; Bahk, Mieoakg; Berzins, Baibaf; Andrasik, Michelea; Mugavero, Michael J.h; Rao, Deepaa,i

Author Information
doi: 10.1097/QAD.0000000000002212

Abstract

Introduction

African-American women are over-represented and over-burdened among women living with HIV in the United States [1–3]. Despite representing only 13% of the total female population, they account for 61% of new HIV diagnoses among women in the United States [4]. African-American women living with HIV are also less likely to be on antiretroviral therapy (ART), more likely to initiate ART late, and more likely to discontinue ART early [5–7], resulting in higher rates of morbidity and mortality than among other women living with HIV [8,9]. Improving rates of viral suppression in this population would reduce morbidity, mortality, and the risk of onward transmission of the virus [10].

African-American women living with HIV have multiple marginalized social identities at the intersection of race, sex, and health, and may therefore be especially vulnerable to HIV stigma [11]. HIV stigma is the co-occurrence of labeling, stereotyping, separating, status loss, and/or discrimination associated with HIV in the context of power imbalance [12]. HIV stigma has several dimensions. Enacted stigma refers to an individual's actual experiences of prejudice and discrimination because of their HIV status. On the other hand, internalized stigma refers to an individual's acceptance of negative attitudes or beliefs related to their HIV status [13]. Stigma can lead to loss of relationships, employment, education, and housing; reduction in help-seeking or treatment-seeking behavior; negative emotional and behavioral changes; and poor health outcomes [14–19]. HIV stigma has consistently been shown to be associated with higher rates of depression, lower social support, and lower levels of treatment adherence [20–24]. Among African-American women in particular, HIV stigma has been associated with isolation [25,26], decreased psychological functioning [27], and symptoms of depression [26,28,29].

Recent cross-sectional analyses have suggested a plausible link between HIV stigma and poor viral control among African-American women receiving treatment for HIV [30]. However, to date no study has established prospective associations between changes in individual dimensions of HIV stigma and subsequent changes in viral load in this population and no study has identified potential mechanisms for these changes. The primary objective of this study was to assess the relationship between multidimensional HIV stigma and viral load, over time, in a sample of African-American women receiving treatment for HIV. The secondary objective was to explore social support and depressive symptoms as potential mediators of this relationship.

Methods

We analyzed longitudinal data from the Unity Study, a multisite randomized controlled trial testing the effectiveness of a behavioral intervention to reduce HIV stigma among African-American women living with HIV [31]. Trial and intervention methods, alongside primary outcome results, are described in detail elsewhere [32]. From May 2013 to October 2015, African-American women living with HIV were recruited from three clinical sites: the Northwestern University HIV clinic and the Ruth M. Rothstein CORE Center (CORE) in Chicago, Illinois, and the University of Alabama, Birmingham 1917 HIV clinic (UAB) in Birmingham, Alabama. Women were eligible for the Unity Study if they self-identified as African-American, were at least 18 years old, were living with HIV, and were receiving HIV services from one of the three clinical sites. Because research suggests that immigrant Black Americans have unique experiences related to HIV and HIV stigma [33–35], women were excluded from the Unity Study if they were foreign-born and had lived in the United States for less than 10 years.

Participants provided written consent to participate in the study and signed Health Insurance Portability and Accountability Act of 1996 authorizations allowing access to their medical records. Sociodemographic and psychosocial data were collected during up to six study visits, via tablet-based audio computer assisted self-interview (ACASI), at baseline, postintervention, and 4, 6, 8, and 12 months postintervention. Relevant clinical data, including data from HIV-1 RNA levels (viral loads) and CD4+ T-lymphocyte counts, were extracted from participant medical records over the course of the study. Participants’ study visit data were included in the present analysis if participants reported having a prescription for ART at the time of the given study visit to ensure modifiability of participant viral load via treatment.

All Unity Study procedures were approved by the University of Washington Institutional Review Board and by institutional review boards at each clinical site. The Unity Study was registered under Clinicaltrials.gov number NCT01893112.

Outcome

The primary outcome of interest was viral load, parameterized as the log of the mean of all viral loads following a given study visit (time = t), prior to any subsequent study visit (time = t + 1) or 180 days poststudy visit, whichever came first. Figure 1 describes the structure of the data and the relevant time intervals. We took the log to reduce the skew of the variable, as recommended for viral load data [36]. Most study visits were followed by another study visit within 60 days; we used the 180-day interval to bound viral loads collected after a participant's final study visit. The secondary outcome of interest was durable viral suppression, defined as having all viral loads under 200 copies/ml following a given study visit (time = t), prior to the subsequent study visit (time = t + 1) or 180 days poststudy visit, whichever came first. As a sensitivity analysis, for both log mean viral load and viral suppression, we also restricted our analysis to viral loads collected within 90 days following a given study visit or prior to the subsequent study visit, whichever came first.

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Fig. 1:
Data structure and approach to linking viral loads with study visits.

Predictor

The predictor of interest was self-reported HIV stigma at each study visit, measured using the 14-item Stigma Scale for Chronic Illness (SSCI). The SSCI measures enacted and internalized stigma and has been validated for use with African-Americans living with HIV (Cronbach α = 0.93) [37,38]. It includes statements like, ‘Because of my illness, people were unkind to me,’ related to enacted stigma, and ‘I felt embarrassed about my illness,’ related to internalized stigma. All items used a 5-point Likert-type scale ranging from 1 = ‘Never’ to 5 = ‘Always’. An overall HIV stigma score, ranging from 14 to 70, was created by summing SSCI scale responses at each study visit. As secondary predictors of interest, we also summed the SSCI sub-scales for enacted and internalized stigma, both ranging from 7 to 35. Higher scores indicated greater HIV stigmatization.

Mediators

The first mediator of interest was perceived social support at each study visit, measured using the emotional/information support and positive social interaction subscales of the Medical Outcomes Study-Social Support Survey (MOS-SSS). The MOS-SSS assesses the degree to which respondents’ interpersonal relationships serve particular support functions in their lives and has been used extensively in chronic disease contexts [39]. The second mediator of interest was depressive symptom severity at each study visit, measured using the 8-item Patient Health Questionnaire (PHQ-8) [40]. This is an abridged version of the PHQ-9 with the suicidality item omitted. The PHQ-9 is commonly used to screen for and monitor depression [41]. Summary social support and depressive symptom scores were created by taking the sum of scale responses. Higher scores indicated greater perceived social support and greater depressive symptom severity, respectively.

Covariates

Covariates were chosen to account for potential observed confounding. These included Unity Study arm (binary), time from baseline in months (continuous), the interaction of arm and time, study site (Northwestern University HIV clinic, CORE, or UAB), years of age (continuous), years since HIV diagnosis (continuous), level of education (less than high school, high school degree or equivalent, some college, or college degree and beyond), occupation (employed, homemaker, student, or other), and number of children (none, 1–3, or 4+). We did not adjust for ART adherence or CD4+ cell count, as we hypothesized that these were part of the causal pathway linking HIV stigma and viral load [42].

Analysis

Descriptive analyses, including t tests and χ2 tests, were conducted to summarize and compare participant characteristics at baseline, stratified above and below the mean overall HIV stigma score at baseline. We then assessed missingness in the data and used linear and logistic regression models to look for associations between participant characteristics and patterns of missing data, including study loss to follow-up, to better understand whether data were missing at random. We next used bootstrapping-based expectation-maximization multiple imputation to impute missing data. All available covariates along with prior and subsequent viral loads were included as predictors in the imputation. We imputed ten datasets and assessed fit using overimputation diagnostic plots. Imputed viral loads were logged and dichotomized, and scale scores were calculated from individually imputed items, after the imputation and prior to inferential analysis. Scale scores were standardized so that 1-U changes in the scale scores corresponded to increases or decreases of 1 SD.

We used four types of generalized linear mixed effects models to estimate prospective associations between HIV stigma and primary and secondary viral load outcomes. Models 1a and 1b used overall HIV stigma as the predictor of interest, treating HIV stigma as unidimensional. Models 2a and 2b used internalized and enacted stigma as the predictors of interest, dividing HIV stigma into the two distinct dimensions available in the SSCI measure. Models 1a and 2a used standardized person-time stigma scores as predictors, producing population average estimates that combined within-subject and between-subject differences in stigma. These models did not distinguish between changes in stigma across individuals and changes in stigma within the same individual over time. Models 1b and 2b used Mundlak (within-between) correction, including both the subject-level mean HIV stigma and subject-time deviations from the subject-level mean as predictors [43]. These models explicitly distinguished between changes in stigma across individuals (between-subject) and changes in stigma within the same individual over time (within-subject). Mundlak correction minimizes observed and unobserved time-invariant confounding because each participant serves as her own control, and has been shown to outperform traditional random and fixed effects models [44]. For each model, we considered three levels of covariate adjustment: unadjusted, adjusting for trial arm, time, and their interaction, and fully adjusting for all covariates. Models with the log mean viral load outcome used the Gaussian family and identity link, while models with the durable viral suppression outcome used the binomial family and logit link. All models included a random subject-specific intercept. All models were estimated on each of the ten imputed datasets, and Rubin's rules were used to pool coefficient and standard error estimates [45]. Finally, we used the estimates from Model 2a to calculate and plot the predicted geometric mean viral loads and probabilities of viral suppression over the observed range of standardized enacted HIV stigma sub-scale scores (−1 SD to +2 SD), with all other covariates at their means [46].

To estimate the indirect effects of overall HIV stigma on viral load via social support and depressive symptom severity, we conducted two causal mediation analyses. We used two sets of linear mixed effects models for each. The first set estimated associations between overall HIV stigma and the respective mediator, while the second set estimated associations between the respective mediator and log mean viral load, adjusting for overall HIV stigma. As above, all models used random subject-specific intercepts, and all were fully adjusted for the same set of potential confounders. Causal mediation analysis proceeded first by predicting the mediator given contrasting predictor values; we contrasted overall HIV stigma from −1 to +1 SD. It then predicted the outcome given the predictor, both with and without the mediator; took the difference of these predicted outcomes to estimate the mediated effect; and finally bootstrapped to estimate uncertainty [47]. Each causal mediation analysis was run using 1000 simulations, on all ten imputed datasets. Final estimates were pooled using Rubin's rules [45].

All analyses were performed in version 3.4.0 of R (R Foundation for Statistical Computing, Vienna, Austria) [48]. Multiple imputation was performed using version 1.7.5 of the Amelia II package [49] and causal mediation was performed using version 4.4.6 of the mediation package [50].

Sensitivity analyses

All models were also estimated using complete case analysis to test the sensitivity of the results to missing data. We also tested an alternative parameterization of the viral load outcomes, considering only viral loads collected within 90 days following a given study visit, instead of 180 days, strengthening the temporal link between HIV stigma and viral load.

Results

Two hundred and thirty nine participants were enrolled in the Unity Study. Two hundred and thirty four participants had a prescription for ART and were included in this analysis. Table 1 summarizes participant characteristics at baseline, stratified above or below mean overall stigma (32.89, SD: 13.15). Mean internalized stigma at baseline was 19.13 (SD: 7.34), and mean enacted stigma was 13.74 (SD: 7.08). One hundred and twenty nine participants had below-average overall stigma scores, while 105 had stigma scores above the mean. Mean age was 46.7 years (SD: 10.5); individuals with higher overall stigma scores were more likely to be older than individuals with lower scores (P = 0.026). Compared with individuals with lower overall stigma scores, individuals with higher stigma scores were more likely never to have been married (44.2% vs. 30.5%, P = 0.021) and more likely to be from the CORE site (47.6 vs. 26.4%, P = 0.003). Individuals with higher stigma scores reported more days of missed ART doses in the previous month (P = 0.007), lower social support (P = 0.001), and higher depressive symptom severity (P < 0.001). They had similar CD4+ cell counts (overall mean 604.3 cells/μl, SD: 371.1), but they had higher log mean viral load (P = 0.006) and were less likely to be virally suppressed (70.5 vs. 85.2%, P = 0.077). Between 0 and 18 (7.7%) participants were missing covariate data at baseline, while 136 (58%) did not have eligible viral loads over the time period from baseline to the next study visit. Participants completed a mean of 4.3 study visits (SD: 1.9) with 42% (n = 98) completing all six study visits. Older participants, separated/divorced participants, and participants with higher social support were slightly more likely to complete all six study visits. Eligible viral loads were linked to 568 out of 1016 (55.9%) total participant-study visits; the other 44.1% were imputed in the primary analysis.

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Table 1:
Participant descriptive statistics at baseline (n = 234).

Table 2 is a summary of estimates from the generalized linear mixed effects models evaluating associations between HIV stigma and viral load. Estimates were consistent across the three levels of adjustment; only fully adjusted estimates are presented in Table 2. In Model 1a each SD increase in overall HIV stigma is associated with an increase in log mean viral load [adjusted β coefficient: 0.24, 95% confidence interval (CI): 0.07, 0.41] and reduced odds of durable viral suppression [adjusted odds ratio (aOR): 0.69, 95% CI: 0.52, 0.91]. Model 1b, which decomposed this association into between-subject and within-subject effects, found that between-subject differences in overall stigma were associated with log mean viral load (adjusted β coefficient: 0.39, 95% CI: 0.11, 0.67) and viral suppression (aOR: 0.58, 95% CI: 0.40, 0.85), while within-subject differences were not. In Model 2a, where internalized and enacted HIV stigma subscales were evaluated as separate predictors, enacted stigma was associated with log mean viral load (adjusted β coefficient: 0.44, 95% CI: 0.24, 0.64) and viral suppression (aOR: 0.62, 95% CI: 0.43, 0.90), whereas internalized stigma was not. Finally, Model 2b decomposed these subscale associations into between-and within-subject effects, and found that both between-subject differences in enacted stigma (adjusted β coefficient: 0.74, 95% CI: 0.33, 1.15) and within-subject differences in enacted stigma (adjusted β coefficient: 0.34, 95% CI: 0.10, 0.58) were associated with log mean viral load. Only between-subject differences in enacted stigma were associated with viral suppression (aOR: 0.51, 95% CI: 0.29, 0.89). Complete model estimates, including measures of association for confounders, are presented in Supplemental Digital Content 1 (Table), https://links.lww.com/QAD/B468; key models are highlighted in green.

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Table 2:
Generalized linear mixed effects model estimates of associations between HIV stigma and viral load.

Figure 2 presents predicted geometric mean viral loads and predicted probability of viral suppression, with CIs, given the observed range of enacted stigma. Under these counterfactual scenarios, the predicted geometric mean viral load rose from 49.90 (95% CI: 33.82, 70.71) at low levels of enacted stigma to 179.64 (95% CI: 106.36, 303.41) at high levels of enacted stigma. The predicted probability of viral suppression fell from 90.47% (95% CI: 84.90, 96.05%) at low levels of enacted stigma to 71.05% (95% CI: 54.77, 87.33%) at high levels of enacted stigma.

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Fig. 2:
Predicted geometric mean viral load and probability of viral suppression by levels of enacted stigma.

Table 3 presents parameter estimates from the mediation models estimating direct and indirect effects of overall HIV stigma on log mean viral load, via social support and depressive symptoms. Although overall HIV stigma was found to be negatively associated with social support in the first step of the first mediation model (adjusted β coefficient: −0.32, 95% CI: −0.39, −0.25), social support was not associated with log mean viral load independently of stigma in the second step. This resulted in an estimated direct effect of 0.40 (95% CI: −0.01, 0.81) and an average causal mediation effect (ACME) of 0.09 (95% CI: −0.02, 0.20), suggesting that 19% (95% CI: −67%, 104%) of the effect of overall HIV stigma on log mean viral load was mediated by social support. The estimated indirect effect was not statistically significant. In the first step of the second mediation model, overall HIV stigma was found to be positively associated with depressive symptoms (adjusted β coefficient: 0.50, 95% CI: 0.43, 0.55), though again depressive symptoms were not independently associated with log mean viral load in the second step. This resulted in an estimated direct effect of 0.48 (95% CI: 0.03, 0.93) and an ACME of 0.00 (95% CI: −0.19, 0.19), suggesting that 0% (95% CI: −82, 82%) of the effect of stigma on log mean viral load was mediated by depressive symptoms. Again, the estimated indirect effect was not statistically significant.

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Table 3:
Mediation estimates of direct and indirect effects of overall HIV stigma on log mean viral load, via social support, and depressive symptoms.

Supplemental Digital Content 1 (Table), https://links.lww.com/QAD/B468 presents results of sensitivity analyses, including the complete case analyses, assessments of the alternative parametrization of viral load, and the test of the assumption that all missing viral loads were not virally suppressed. All results from sensitivity analyses were consistent with the primary results.

Discussion

The current study examined longitudinal associations between HIV stigma and viral load among African-American women receiving treatment for HIV. Results suggest that HIV stigma and viral load are closely linked. Specifically, women with higher overall HIV stigma scores were more likely to have higher subsequent viral loads than women with lower overall HIV stigma scores. Within-person changes in overall HIV stigma were not significantly associated with subsequent viral load, but assessment of internalized and enacted HIV stigma as independent predictors revealed that both between-person and within-person differences in enacted HIV stigma were significantly associated with subsequent viral load. This suggests that ongoing experiences of stigma related to HIV may have immediate, deleterious effects on HIV-related health and hinder the transition to viral suppression in this population. Aside from treatment nonadherence, the mechanism for these effects is not clear; our analyses did not identify social support or depressive symptoms as statistically significant mediators of the relationship between HIV stigma and viral load.

Our results reinforce the notion that the unique dimensions of HIV stigma are differentially associated with patient health and wellbeing [51]. Although internalized stigma may be predictive of affective or cognitive outcomes, enacted HIV stigma may be uniquely tied to HIV-related physical health. Other studies in the United States have found associations between enacted stigma and HIV symptoms [52], CD4+ cell count [51], and viral load [53]. Significantly, these studies also failed to identify mediators or moderators of this relationship, aside from treatment nonadherence, suggesting a more direct relationship between the stress of enacted stigma and subsequent health outcomes [51,54]. Future research might elucidate this relationship by testing other possible mediators, including care-seeking behavior and clinical care engagement, and by measuring changes in behavior over short periods of time immediately following experiences of HIV stigma.

Our findings have significant implications for future interventions. African-American women are at higher risk for HIV-related morbidity and mortality [8,9], and ongoing experiences of HIV stigma appear to interfere with maintenance of or progress towards viral suppression in this population. As such, interventions should aim to alleviate internalized stigma among women living with HIV, as well as target the sources of stigma to prevent future experiences of stigma. Stigmatization of African-American women living with HIV may come from many places – friends, family, partners, healthcare workers, community members, and societal structures – and may magnify existing vulnerabilities on account of disadvantaged race and sex [55]. Notably, the source of the stigma matters; evidence suggests that stigmatization by healthcare workers may be especially detrimental to HIV treatment outcomes [53].

Our results are supported by our use of longitudinal data and the prospective analysis in which all associations were assessed between psychosocial predictors measured prior to the viral load outcomes. However, the study had several limitations. First, almost half of the viral load outcomes were missing. This was a structural missingness, as study visits were more frequent than routine viral load assessments; thus, many visits were not followed by eligible viral loads. The use of multiple imputation in our analysis helped to avoid the bias and loss of efficiency that can result from missing data [56], Moreover, our use of multiple imputation had the advantage of leveraging all available self-reported psychosocial data and ensured that measures of association were prospective. It should be noted that sensitivity analyses demonstrated that the findings were consistent regardless of how we treated the missing data. Second, we could not adjust for several variables that are known to be strongly associated with viral load, including alcohol/substance use and homelessness/housing insecurity [57,58]. In response, we used the fixed-effects estimator as part of the Mundlak correction to attempt to account for all observed and unobserved time-invariant confounding [44]. Third, we did not adjust for possible observed or unobserved time-varying confounders. Potential time-varying confounders would include participants’ prior HIV stigma status and their prior viral loads. Future analyses may consider applying g-computation or instrumental variables to account for possible time-varying confounding [59,60]. Fourth, our models tested a particular pathway, assuming that HIV stigma would affect viral load [13]. It is possible that the true pathway is from viral load to stigma, perhaps via visible symptoms of ill health or increased exposure to the health system. Further analyses are needed to confirm the direction of causality between HIV stigma and viral load. Fifth, ordinary least-squares regression with a logged outcome (log-OLS) may be imprecise or even biased compared with generalized linear models (GLM) under certain circumstances [61]. Further studies may consider GLMs with log link and gamma probability distribution that directly accommodate the positively skewed distribution of the outcome. However, log-OLS has been shown to outperform GLM with heavy-tailed distributions, as was the case in this study [61]. Sixth, though we identified a link between ongoing experiences of HIV stigma and higher viral loads, our data did not identify the sources of these experiences. Future study is needed to assess whether stigmatization by particular sources (e.g., family members, healthcare workers) is especially detrimental in this population. Finally, this was a secondary analysis of data from a sample of women on treatment who participated in a group-based stigma reduction intervention. They may have differed from the broader population of African-American women living with HIV in substantive ways; for example, they may have had higher overall HIV stigma levels, or self-selected into the intervention due to issues with low social support. Our findings should be confirmed using a larger, representative sample of African American women living with HIV.

In conclusion, we assessed the relationship between HIV stigma and viral load among African-American women receiving treatment for HIV. Our study indicates that ongoing experiences of HIV stigma may have negative effects on viral load in this population over relatively short periods of time. These effects do not appear to be mediated by social support or depressive symptoms. To ensure that interventions to alleviate stigma among African-American women living with HIV have a beneficial impact on patient health, they may need to broaden their focus to target the sources of stigma, rather than just those who are stigmatized.

Acknowledgements

C.G.K., L.L., D.H., and D.R. conceived of the study. C.G.K. developed the analytic plan and conducted the analysis with support from L.L. and D.H. D.R. led the trial that collected data used for this study, with support from D.H., P.E.N., J.M.T., J.M.S., S.E.C., M.B., B.B., M.A., and M.M. C.G.K. drafted the article, and all authors contributed to revisions. All authors read and approved the final article.

We would like to acknowledge and thank the participants of the Unity study. We also thank Dr Brandon Guthrie and Dr Marco Carone, both at the University of Washington, for valuable comments on early analytic plans.

Financial support was provided by NIMH 1R01MH098675-01 (PI: Rao) and NIMH 1F31MH112397-01A1 (PI: Kemp).

Conflicts of interest

There are no conflicts of interest.

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Keywords:

African-American; HIV; longitudinal; stigma; viral load; women

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