Children with HIV infection (HIV+) are at neuropsychological risk, with studies showing an association of HIV with cognitive and motor dysfunction in perinatally infected children [1,2]. However, few studies have evaluated neuropsychological outcomes among perinatally infected children who received combination antiretroviral treatment (cART) prior to 3 years of age in resource-poor settings. Although cART has increased survival of HIV infected children into adolescence and young adulthood, alone it is insufficient to reverse the neurodevelopmental consequence of HIV infection .
The purpose of this observational multicenter longitudinal study is to compare neuropsychological outcomes cross-sectionally among the perinatally HIV+, HIV exposed uninfected (HEU), and HIV unexposed uninfected (HUU) children in sub-Saharan Africa with language and cultural differences. HUU children without a history of significant risk for brain injury were included as a reference group to better understand the neuropsychological risk from HIV infection versus exposure but not infection. We present the neuropsychological performance of HIV+ children compared with age-matched HEU and HUU controls, after statistically adjusting for a number of descriptive and contextual factors that may influence neuropsychological outcomes. By doing so, we address a key gap in the research literature, by evaluating whether neuropsychological function in African children with HIV is diminished at school age (5–12 years) even when ART is initiated at an early age (<3 years) with good clinical monitoring and treatment support.
Children were recruited into three cohorts: HIV+ children (N = 246) participating in P1060 version 5.0, HEU (N = 183), and HUU (N = 182). All P1060 study children (HIV+) were eligible for enrollment in the current study with over 95% participating. These were HIV status-verified and medically well characterized children from 5 to 11 years of age at enrollment in the present follow-up observational study. These P1060 study children were perinatally infected children from a randomized controlled trial (RCT) comparing ART nevirapine (NVP) versus lopinavir/ritonavir (LPVr) upon HIV diagnosis in infancy or very early childhood [4–6]. Neuropsychological comparison (Table 1) between the ART NVP versus LPVr for the children with HIV will be presented in a separate report to be published at a later time. By the time of enrollment in the present observational follow-up study, most HIV children in the NVP arm had been switched to second-line cART, and 96% were virally suppressed at the time of baseline neuropsychological assessment. In the current study, HIV+ children had good immunological and virological status, with over 95% having CD4+% at least 25% and viral load 400 copies/ml or less (Table 2). However, most of the HIV+ children in the current study presented with major signs of disease at the time of enrollment in the ART clinical trial in early childhood, and therefore were documented to have had these prior occurrences when enrolled in the present follow-up study [WHO stage I = 38 (15%); stage II = 58 (24%); stage III = 137 (56%); stage IV = 13 (5%)] (Table 2).
Sample sizes for the HEU and HUU cohorts were determined on the basis of sample size needed for 80% statistical power calculated using a prior study comparing HIV+, HEU, HUU Ugandan cohorts for the principal Kaufman Assessment Battery for Children, 2nd edition (KABC-II; mental processing index or MPI), Tests of Variables of Attention (TOVA; D prime score) and Bruininks–Oseretsky Test, 2nd edition (BOT-2) of motor proficiency total score . Enrollment of the HUU children took place at vaccination and outpatient treatment clinics at each study site, consisting of a convenience sample age matched to the P1060 HIV+ children . These children were medically screened for prior hospitalizations that could involve brain injury (e.g. cerebral malaria, meningitis, head trauma) or severe malnutrition, and also excluded if they screened positive for any developmental disability (as screened using the MICS4 Disability Questionnaire given to the principal caregiver). HEU children were born to HIV+ mothers recruited from the same households, extended families, or neighborhoods/communities as the P1060 HIV participants. HEU children were not excluded on the basis of prior medical history or indication of developmental disability. The HIV status of the mother at the time of birth was verified in the medical record.
Enrollment took place from early October 2013 to mid-December 2014. The six study sites were Wits RHI Shandukani Clinic, Johannesburg, South Africa (Johannesburg RSA; principal local languages of Sesotho and Zulu); Chris Hani HIV Unit, Soweto, South Africa (Soweto RSA; principal local languages of Sesotho and Zulu); Family Clinical Research Unit, Cape Town, South Africa (Tygerberg RSA; principal local languages of Xhosa, Afrikaans, English); Kamuzu Central Hospital HIV Clinic (Lilongwe Malawi; principal local language of Chichewa); Makerere University – Johns Hopkins University (MU-JHU) Research Collaboration Clinic at Mulago National Referral Hospital (Kampala Uganda; principal local language of Luganda); and Parirenyatwa General Hospital (Harare Zimbabwe; principal local language of Shona).
The institutional review board (IRB) approval for this study was obtained from the human patients’ protection in the research regulatory committee at each study site, and where applicable the corresponding ministry of health in the host country, and the university partner in the United States for each study site. Informed consent was obtained from parents or primary caregivers with additional assent from children more than 7 years based on country regulations.
Participants were assessed with a neuropsychological assessment battery of tests at entry and at 2 yearly follow-up visits. The current study reports only the cross-sectional comparisons of the baseline (study entry) assessment among the study cohorts. A longitudinal comparison among cohorts of all three assessments over the 2-year study period will be presented separately in an article to be published at a later time. Additional data were collected regarding the demographic and socioeconomic status (SES) of the household, the child's anthropometric measures (Table 3), illness history, and medications. Caregivers responded on the Hopkins Checklist for Depression/Anxiety (HSCL-25) where anxiety or depression symptoms were rated from not occurring at all (0) to occurring often (3). Item rating averages are presented for these measures in Table 3, Behavior Rating Inventory of Executive Function (BRIEF) school-age, United Nations Children's Fund (UNICEF)-sponsored Multiple Indicator Cluster Surveys, 4th edition (MICS4) questionnaire for child development, and MICS4 child disability questionnaire. Testers were blinded to the child's exposure group. Table 1 lists the key outcomes for each of the tests described below.
Kaufman Assessment Battery for Children (second edition)
The KABC-II was the principal test for cognitive ability outcomes . It has also been validated in the sub-Saharan African context [8–11], and previously adapted for use in pediatric HIV research in the current study site countries of Uganda and South Africa [1,12–17]. Using the Luria model for neuropsychological assessment within the KABC-II, the primary outcome variables were the global scores of sequential processing (memory), simultaneous processing (visual-spatial processing and problem solving), learning (immediate and delayed memory), planning (executive reasoning), delayed recall, nonverbal index (NVI) (subtests not dependent on the understanding of instructions in English) and Mental Processing Index (MPI) (a composite of the principal cognitive performance domains). The KABC-II offers the option of scoring using time points, but these were not used in the present assessment.
Bruininks–Oseretsky Test of motor proficiency, (2nd edition)
The BOT-2 is one of the most comprehensive instruments for motor assessment , previously used for pediatric HIV assessment in Uganda as motor impairment often accompanies HIV in children [1,19]. The short form of this test includes two or three items pertaining to fine motor precision, fine motor integration, manual dexterity, upper limb coordination, bilateral coordination, balance, running speed and agility, and strength. These are combined into total composite score of motor proficiency, standardized by age and sex using American norms. This total standard score for motor proficiency was the only outcome for this test used in the present analyses.
Test of Variables of Attention (version 8.0)
The TOVA is a computerized visual continuous performance test used in the diagnosis and monitoring of children and adults with attention deficit disorders (www.tovatest.com) . This test consists of the rapid (tachistoscopic) presentation of a large geometric square on the computer screen with a smaller dark box either in the upper position (signal) or lower position (nonsignal). The child is asked to press a switch held in the preferred hand as quickly as possible in response to the signal (measuring vigilance attention), but to withhold responding to the nonsignal (measuring impulsivity). Following spoken instructions in the local language and practice trials, the TOVA takes about 11 min for children 5–5.5 years of age, and 22 min to administer for children 5.5 years of age and older. The TOVA had been adapted for pediatric HIV research in Uganda [1,13,15,19,21].
The primary outcome variables were response time variability (a sensitive indication of inattention), average response time to signal, percentage commission errors (impulsivity), percentage omission errors (inattention), an attention deficit–hyperactivity disorder (ADHD) index score (missed signals in proportion to incorrect responses to nonsignal), and a signal detection measure of overall test performance called D prime (correct ‘hits’ to signal in proportion to correct nonresponses to nonsignal).
Behavior Rating Inventory for Executive Function, parent school-age
The BRIEF for school-age children (6–18 years) has 86 items that are read out loud to the parent or guardian and evaluates behavioral and cognitive behavior problems related to disruption of executive functions of the brain . It has previously been adapted for use in pediatric HIV research in Uganda [23,24]. The BRIEF was translated into the principal local languages at all study sites (with permission of the publisher including approval of the back translation by test authors). The eight scales form two broad indexes, behavior regulation index (BRI) with three scales, Metacognition Index (MI) with five scales, and these (BRI + MI) are combined into a Global Executive Composite (GEC) Score. The higher the score, the more day-to-day behavior problems related to executive function as reported by the parent/caregiver.
Hopkins Symptoms Checklist (25-items) for depression/anxiety
The HSCL-25 is used to assess severity of caregiver depression (15 items) and anxiety (10 items) [25,26]. The HSCL-25 has been used in studies of emotional well being of caregivers of Ugandan children with or affected by HIV [23,27]. The HSCL was translated in the principal local languages at each of the study sites and read out loud to the mother or principal caregiver in a private setting.
UNICEF Multiple Indicator Cluster Surveys, 4th round questionnaire for child development and for child disability
It is crucial to control for quality of home environment whenever measuring developmental and neuropsychological outcomes in at-risk children. At enrollment, we used portions of the MICS4 administered to the principal caregiver of the child. We used the early childhood development (ECD) portion of this questionnaire for children under 5 years (17 items) as a measure of quality of child development environment. We also used the MICS4 child disability questionnaire, derived from the Durkin 10-question questionnaire [28,29], in screening HUU children for eligibility as a control group in the current study.
Using an assessment of SES previously used in pediatric research in Uganda , information on other members of the household, parental/caregiver status and their education and occupation, physical quality of home environment (e.g. electricity, water source), material possessions (e.g. working refrigerator), and source of income.
Medical history and physical development
Medical history and anthropometric measurements (weight, height, BMI, mid-upper arm circumference) were collected at the study visit. Anthropometric measures were standardized using WHO norms to computer z scores. The medical history questionnaire included questions on health status (targeted diagnoses, signs/symptoms) and was collected at the clinic before neuropsychological assessment to help ensure that the child was well enough to test.
Descriptive statistics were used to summarize caregiver and child characteristics for all three cohorts, as well as HIV disease status for the HIV+ cohort (Tables 2 and 3). Comparability across study cohorts was tested using the Kruskal–Wallis nonparametric tests (Table 3) and chi-square tests. Linear regression analyses using generalized estimating equations (GEE models) (Tables 4 and 5) were performed to assess differences among study cohorts, first without adjustment followed by adjustment for clinical site, age and sex (partially adjusted models), and finally adjusting for personal and family characteristics (fully adjusted models). Least squares mean estimates by cohort were computed for unadjusted, partly adjusted (adjusted for sex, age, clinical site), and fully adjusted models (Tables 4 and 5).
The associations between each potential confounder and each outcome measure were first assessed using unadjusted GEE models. Final regression models were developed in two steps. Initially, all potential confounders with univariate P values less than 0.20 were included in a multivariable model. Clinical site, age, and sex were included in these models regardless of the univariate P values. We excluded sites by cohort interactions based on our previous findings, which suggested their magnitude would not greatly influence interpretation. If more than one related measure was significant in the univariate analysis (e.g. for those related to fuel – ranking of fuel, access to electricity, number of fuel sources), we selected one out of the group for the multivariable model. In such cases, in addition to selecting by ease of interpretation, we also performed multiple regressions including the related factors to assess which had the smallest P value (most highly significant).
All covariates for which the P values remained less than 0.20 were retained in the final multivariable model, along with cohort, clinical site, age, and sex. Backward selection was used until only covariates with P less than 0.20 remained in addition to age, sex, cohort, and site. A core multivariable model for each test domain was developed in this way for key outcomes and then subsequently used on the remaining outcomes in the domain. For the KABC-II, the key outcome was the MPI, although a separate model was run for the NVI. The D-prime score was the key outcome for the TOVA, and the standardized total motor proficiency score was the only outcome for the BOT-2. The key outcome for BRIEF was the GEC. Table 1 has a list of the principal outcomes for each test along with the covariates from Table 3 that were retained in the adjusted analyses for each outcome for Tables 4–6.
A final GEE regression analysis compared HIV children with early (at <12 months of age) versus later ARV medication initiation, controlling for site, age, sex, and various family characteristics (Table 6). Tests of statistical significance were two-sided and, unless noted, 5% error rates were used for hypothesis testing. The data analysis for this paper was generated using SAS software, Version 9.4 (SAS/STAT 14.1) of the SAS System for Unix. Copyright © 2012 SAS Institute Inc. SAS and all other SAS Institute Inc. product or service names are registered trademarks or trademarks of SAS Institute Inc., Cary, NC, USA.
A total of 615 participants were enrolled at the six research sites, with 246 in the HIV+ cohort, 185 HEU, and 184 HUU cohorts. However, 611 participants (246 HIV, 183 HEU, and 182 HUU) were eligible for this baseline analysis with a median age of 6.9 years (interquartile range, 6.2–8.1 years). Enrollment by study site is in the Supplemental table, http://links.lww.com/QAD/B183. Four HIV+ children were excluded from the present analyses as they could not complete the test battery (one child was deaf/mute, two were behaviorally uncooperative or disruptive, and one had significant neuromotor disability). When evaluating floor scores (scores at or below the lower limit) for each of the principal outcomes for each neuropsychology test, there were fewer than 3% of participants with floor scores. Aside from the TOVA, which had roughly a 94% completion rate, over 98% of tests were completed on the other tests (KABC-II, BOT-2, BRIEF). Neither validity nor completeness proved a problem in these data. Given the very high level of available valid and complete measures for our principal outcome measures, there was no need to impute for missing scores on the basis of cohort mean values for a given measure. The probability estimates for both the adjusted and unadjusted between-cohort comparisons in Tables 4–6 were adjusted according to the appropriate degrees of freedom to evaluate statistical significance and effect size.
Table 3 shows personal and family characteristics by study cohort. The groups are balanced by sex and age. HIV+ children tended to have caregivers with less schooling and fewer have siblings enrolled. Fewer of the participants in the HIV+ cohort have caregivers who are biological mothers. As expected from the study design, primary caregivers are largely infected with HIV in the HIV+ and HEU cohorts, in contrast to the HUU cohort. The HIV+ cohort's immunological and virological status at study entry was very good, with over 95% having CD4+% at least 25% and viral load 400 copies or less.
The World Health Organization (WHO) classification for HIV disease is progressive, and most participants entered the P1060 ART clinical trial study in early childhood with more advanced HIV disease. Up to 9% of study participants reported a serious illness prior to entry in the current study, with the most prevalent across cohorts being premature birth. More participants in the HIV+ cohort reported a history of severe malaria, low birth weight, tuberculosis and malnutrition, among others illness. A fair number (39%) of HIV+ participants reported cozole/trimethoprim use (39%). Other medications were reported for fewer than 2% of each study cohort. Weight and height z-scores (standardized using WHO norms based on age and sex) were significantly lower for the HIV+ children compared with the HEU and HUU cohorts (Table 3). The disability scores were higher for the HIV+ compared with the HEU and HUU cohorts. However, the MICS4 child development environment scores were comparable.
In the between-group adjusted comparisons presented below and in Tables 4–6, the covariates for which we adjusted from Table 3 were dependent those which were retained based on the significance of their loadings in the initial stage of the stepwise regression analyses. Unadjusted and adjusted mean scores and differences among cohorts are available in Tables 4 and 5. Only significant P values are reported in this narrative so as to make the presentation of the results less cumbersome and to conserve space. In adjusted between-group differences, HIV+ children performed significantly more poorly than both the HUU and HEU cohorts on all the global scales of the KABC-II (Tables 4 and 5; Fig. 1). These included sequential processing (working memory) (P < 0.001), learning (P < 0.001), delayed recall (P < 0.001), simultaneous processing (visual-spatial analysis) and planning (reasoning) (P < 0.01), and the composite scores of these four domains [MPI (P < 0.001)], as well as the composite for those subtests not involving language comprehension [nonverbal index (NVI) (P < 0.001)] (Table 4, Fig. 1). There were no significant differences between the HEU and HUU cohorts on any of the KABC-II global measures (Table 5; Fig. 1). Between-group differences among the groups were consistent across all six study sites (HIV < HEU = HUU), but overall performance on the KABC-II, irrespective of HIV exposure group, differed significantly between sites (Figs. 2 and 3), resulting at times in significant group by site interaction effects. Among the KABC-II adjusted raw score comparisons for individual subtests not always included in the global scale measures, HIV+ children performed worse than the HEU and HUU cohorts on the planning/reasoning domain subtests of conceptual thinking, story completion, pattern reasoning, and on the sequential processing (working memory) subtest of hand movements (P < 0.001) (Tables 4 and 5).
For the TOVA, HIV+ children performed poorly compared with the HEU and HUU cohorts on all outcomes pertaining to vigilance attention (percentage omission errors, average response time response time variability, ADHD index, and D prime) (P < 0.001) (Tables 4 and 5; Figs. 3 and 4). The cohorts did not differ significantly on the principal outcome for impulsivity (percentage commission (Table 4; P = 0.09). There was no difference in the TOVA measures between the HEU and HUU children (Table 5; Fig. 2). These between-group differences were consistent for all of the study sites (no significant between-group by site interaction effects), although as with the KABC-II, there were significant overall TOVA performance differences between the study sites (Supplemental table, http://links.lww.com/QAD/B183).
Overall, HIV+ children had significantly lower mean scores on the adjusted BOT-2 performance than the HEU and HUU controls (P < 0.001) (Table 4; Figs 1, 3 and 4). There was no difference between the HEU and HUU children (Fig. 2). Although not significantly so, all the BRIEF global index scores tended to be higher (more behavior problems) for the HIV cohort (Tables 4 and 5).
An important consideration in the current study was whether earlier initiation of ART for the HIV+ children was protective for neuropsychological outcomes. In Table 6, we compare HIV+ children initiated on treatment before 1 year of age, to those initiated on treatment after 1 year of age. We limited this comparison to just the standardized (age adjusted using American norms) global performance measures for our neuropsychological tests. The only significant difference was for the BRIEF GEC composite (P = 0.03), with an advantage in executive function behavior evaluations (lower scores meaning fewer behavior problems) for children initiating ART before 1 year of age. In a subsequent article, we plan to do more thorough analyses of neuropsychological outcomes on the basis of ARV treatment arms and clinical response and parameters for the HIV+ cohort in our study, who all participated in the IMPAACT P1060 RCT [4,5,31]. To summarize, among selected KABC-II, TOVA, BOT-2, and BRIEF outcomes, only the BRIEF GEC score differed at study entry by age of ART initiation (Table 6).
The current study is distinctive in that it used the same assessment protocol in six sub-Saharan Africa study sites in four countries and enrolled children and caregivers in 10 different languages. Although overall neuropsychological performance differed across sites, exposure group differences on our neuropsychological outcomes were remarkably consistent across all six sites, representing a greater level of rigor and reproducibility than documented in ‘single-site’ studies. Furthermore, the current study enrolled HIV+ children from the same ART trial protocol, initiated on treatment at diagnosis (from 3 months to 3 years of age). This provided for an exceptionally well characterized and cared for cohort that was virally suppressed and clinically stable at the time of neuropsychological assessment. A comparison of neuropsychological outcomes on the basis of ART trial study arm for the current study children with HIV will follow in a separate publication.
The present findings filled a key gap in the research literature by characterizing the neuropsychological status of the ART trial children at school-age, who were all initiated on an ART treatment program at an early age (<3 years) with careful clinical and adherence monitoring following treatment initiation, until the present assessment. We did so with a cross-sectional comparison of neuropsychological function of age-matched perinatally exposed HIV+, HEU, and HUU cohorts. Additional longitudinal assessment comparisons of these cohorts across three time points over a 2-year period will follow in a separate publication. Findings from the first assessment comparison are presented here.
Despite the challenges of enrolling appropriate control groups for each study site, the study sites successfully enrolled and completed a 3–4-h battery of tests which included children less than 6 years of age, not yet in school, from low-resource settings, and successfully completed the baseline tests. In this study, HIV+ children performed poorly on the KABC-II including sequential processing, simultaneous processing, learning, planning, delayed recall, NVI, and MPI compared with HIV negative controls (HEU, HUU). Similar findings were reported with significantly poorer performance in executive function tasks, particularly in terms of processing speed [1,32,33], memory , and attention [1,19]. Early studies among younger HIV+ children have also described lower visual–spatial processing scores, which impact on reading, writing, and learning in adolescence .
The present HIV+ participants had received ART at a young age (<3 years) and received continuous clinical and medical monitoring and support in a well resourced clinical trial program (IMPAACT P1060 clinical trial) up until the current study assessment. Nevertheless, the HIV+ cohort still performed significantly worse than their HEU and HUU counterparts on all major neuropsychological outcomes pertaining to cognitive, attention, and motor ability. These findings are similar to our published findings comparing HIV+ (ART-naive school-age Ugandan children with less advanced HIV disease) and HUU Ugandan children using the KABC-II, TOVA, and BOT-2 . However, the neuropsychological performance differences among our exposure groups are more extensive and more significant, particularly for the HIV+ children. This is likely because the HIV+ children came into P1104s baseline assessment proportionately with more advanced HIV disease than those studied by Ruel et al. (2013) where children were ART-naive and not yet ART eligible. In terms of physical growth, HIV+ children in this study were also more stunted and wasted then the HEU and HUU children, which, in the African context, is predictive of the more pervasive neurodevelopmental effects of HIV disease in early childhood [34,35].
Further support of this conclusion is provided in a recently published cohort study of 1383 children 6–8 years old in KwaZulu-Natal, South Africa . Using the KABC-II as one of the tests for providing cognitive performance outcomes, the authors evaluated the association of demographic variables (area of residence, sex, preschool education, HIV status, height for age, and hemoglobin level) and family variables (SES, and maternal and paternal level of education), with children's cognitive performance. Area of residence, height-for-age, and paternal level of education were all statistically significant factors affecting cognitive test scores, whereas HIV status, sex, and SES were not. They concluded that at-risk children in impoverished rural areas with low cognitive scores tended to be stunted (low height-for-age scores), lacked preschool education and were younger, and that parents’ educational level also was important. These factors could overshadow HIV status per se in a highly impoverished rural African-based study population .
These conclusions are consistent with another recent comparison of HIV to HUU an HEU children in an impoverished rural area of Uganda . Using the KABC-II as main outcome, these investigators obtained significantly poorer performance in the NVI composite among HIV+ children compared with their HEU and HUU counterparts. These differences were driven largely by differences in the sequential processing (working memory) and learning global domains. Early initiation of ART, however, combined with subsequent years of schooling resulted in better KABC-II sequential processing outcomes for the HIV+ children . An earlier KABC assessment of Ugandan HIV+ (ARV-naive), HEU, and HUU children noted little difference among these cohorts except perhaps in the domain of simultaneous processing . This is in contrast to a study of HIV+, HEU, and HUU cohorts in the Democratic Republic of the Congo, which observed significantly poorer performance for HIV+ children in KABC sequential and simultaneous processing and in motor proficiency . However, these neurodevelopmental deficits could be mitigated by ART and supportive medical care in the first few years of life [38,39].
The neuropsychological performance of the HEU and HUU children did not differ significantly for our principal study outcomes, unlike other studies where such differences have been noted [37,39,40]. Standards of care varied during pregnancy for women with HIV varied among our country sites, as well as the type of ART used for the prevention of mother-to-child transmission of HIV infection. However, the HEU dyads enrolled for our comparison cohort across all six of our study sites were from other clinical studies where the HIV status of mother and child were perinatally documented. These women tended to receive prenatal care throughout most of their pregnancy at our study clinics. Therefore, they were probably receiving a better quality of medical care for antenatal and postnatal maternal health conditions than might typically be available for pregnant mothers with HIV served at such large public hospitals in the urban setting (e.g. maternal ART and support, maternal and child micronutrient supplements, prompt diagnosis and treatment for anemia, and for symptomatic and asymptomatic malaria, supportive care for continued breast feeding, cotrimoxazole prophylactic for HEU children in the first months of life). Such supportive care, although the standard of care in all of our study settings, are not typically as readily available to non-study women, and could well have boosted the health and early growth and development of our HEU children more than would have been the case in other neurodevelopmental studies comparing HEU with HUU cohorts. These aspects of our HEU cohort enrollment could limit the extent to which our present neuropsychological comparisons for the HEU and HUU children should be generalized to the general population in such African settings.
The current study provides conclusive evidence that African children with HIV are at significant neuropsychological risk, even with early ART initiation and careful medical support. These findings support the conclusions by Boivin et al. in their review. They also documented that although the more severe forms of HIV-associated neurologic deficits are reduced following cART treatment, neurocognitive and behavioral problems can persist and deepen at school age despite effective and sustained plasma viral suppression from an early age. These conclusions were also supported in a review by Laughton et al., and suggest that behavioral interventions are needed in combination with medical treatment and care to fully address the neurodevelopmental needs of children and adolescents in Africa living with HIV. To illustrate, early childhood development caregiver training programs can enhance the developmental milieu of the child with HIV and lead to improved cognitive and social development [42–44]. For school-age children, computerized cognitive rehabilitation training can improve attention, working memory, and problem solving skills for children with HIV [15,21,43].
For future studies with our current study cohorts, our study team is currently proposing combining such behavioral interventions with newer cART medications proven to be effective in penetration of the central nervous system in achieving viral suppression as early as possible in the developing brain. Dolutegravir (DTG), an integrase inhibitor approved for children over 6 years of age, has good CNS penetration effectiveness (CPE), exceeding that of LPV/r [45,46]. Maraviroc (MVC) is a CCR-5 inhibitor, preventing HIV attachment to CCR-5 expressing macrophages and CD4+ T cells. MVC has a CPE of ‘3’, equivalent to efavirenz, and when added to suppressive ART, was associated with neurocognitive improvement in two prospective adult studies [47,48]. MVC also has an anti-inflammatory effect which can enhance neural function diminished by neural inflammation in HIV disease. This is evidenced by the preliminary findings that MVC can improve neurocognition in adult HIV patients with advanced disease . Likewise, recent work in mice suggests that CCR-5 inhibition also increases plasticity and learning, independent of effects on HIV and inflammation .
Our study team is proposing in future work that combining DTG and MVC with computerized cognitive rehabilitation training in school-age children with HIV may provide a means by which neuropsychological impairment can, in part, be remediated. Likewise, initiating DTG and MVC as early as possible with infected infants and combining this with follow-up early childhood development (ECD) enhancement caregiver training for cognitive and nutritional enrichment in the home may better buffer children against neuropsychological impairment at school age. Such cART and behavioral intervention combination treatment plans, initiated as early as possible, should become the new standard of care in the treatment of pediatric HIV disease.
Significant cognitive deficits were documented for the HIV+ cohort across sites. Earlier HIV treatment, neuropsychological monitoring, and rehabilitative interventions are needed. In the current study, we adapted our neuropsychological test battery and caregiver questionnaires to differing cultural contexts at six study sites in four different sub-Saharan countries and across at least ten different local languages. Despite the cross-cultural implementation challenge, our findings clearly establish the feasibility, sensitivity, and robust nature of our neuropsychological outcomes for the principal study aims. Extending the assessment further to 48 and 96 weeks postbaseline assessments will provide a between-exposure group and between ART treatment-arm comparisons of the developmental trajectory over two additional yearly time points. This should bridge the gap in documenting long-term effects of HIV and ART on neurodevelopmental outcomes. Finally, using newer more highly CNS penetrant cART drugs (e.g. DTG, MVC) should be combined with caregiver-based cognitive and nutritional enrichment interventions in the home. These can also be transitioned to computerized/tablet/mobile devices for cognitive training and enrichment at school-age. Such combination ART/behavioral interventions for children with HIV in resource-constrained settings should be initiated as early as possible in children's development, in the hopes of preventing further neuropsychological injury from pediatric HIV disease.
Overall support for the International Maternal Pediatric Adolescent AIDS Clinical Trials (IMPAACT) Network was provided by the National Institute of Allergy and Infectious Diseases (NIAID) of the National Institutes of Health (NIH) under award numbers UM1AI068632 (IMPAACT LOC), UM1AI068616 (IMPAACT SDMC), and UM1AI106716 (IMPAACT LC), with co-funding from the Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) and the National Institute of Mental Health (NIMH). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. Drs E. Pim Brouwers (NIH/NIMH) and Sonia Lee (NIH/NICHD) served as protocol advisors with the research leadership team for P1104s for their respective NIH institutes. We gratefully acknowledge their expertise and counsel during the study. The authors also acknowledge the protocol administrative support provided by J.L. Ariansen from FHI360, as well as the support provided by Dr Elizabeth Petzold throughout the protocol approval, finalization, mobilization, and study initiation process.
M.J.B. as protocol chair designed and directed the study and cowrote the complete first draft of the article. L.B.-M. was lead investigator at the Kampala Uganda study site and cowrote the complete first draft of the article. M.C. directed all statistical analyses and related narrative and constructed the tables and figures for this article, contributed to and approved the final version of the article. B.L. was lead investigator at the Stellenbosch South Africa study site and advised on assessment methodology for this study, as well as the validation process and quality assurance of the assessment process. B.Z. directed the development of the Case Report Forms and all phases of data management and validation checks. C.J. directed the neuropsychological assessments at the Soweto South Africa study site and contributed to and approved the final version of the article. M.B.-D. was lead investigator at the Harare Zimbabwe study site and contributed to and approved the final version of the article. M.R. was responsible for all of the neuropsychological testing at the Johannesburg South Africa study site and approved the final version of the article. N.A. directed patient recruitment and supervised protocol management at the Soweto South Africa study site and approved the final version of the article. L.F. was lead investigator at the Johannesburg South Africa study site and contributed to and approved the final version of the article. H.G. directed patient recruitment and supervised protocol management at the Johanesburgh South Africa study site and approved the final version of the article. P.K. was lead investigator at the Lilongwe Malawi study site and supervised protocol management and approved the final version of the article. K.M. was responsible for protocol oversight, amendment approvals and coordination, enrollment management, and contributed to and approved the final version of the article. I.F.-L. coordinated quality assurance and assessment for the neuropsychological assessment center based at the Uganda study site and contributed to and approved the final version of the article. P.J.-P. served as NIAID scientific advisor to this study and contributed to protocol design and approval and contributed to and approved the final version of this article. J.C. served as project Human Subjects Ethics and Research advisor, protocol advisor, and enrollment advisor for the study and approved the final version of the article. A.V. was lead investigator at the Soweto South Africa study site, served as protocol and scientific advisor, and contributed to and approved the final version of the article. M.C.C. was lead protocol advisor at the Stellenbosch South Africa study site, served as a scientific advisor, and contributed to and approved the final version of the article. P.P. was principal investigator for the P1060 RCT study for the study children with HIV, was a principal contributor to the study concept, protocol design and implementation, grant writing and funding approval, contributed to and approved the final version of the article.
Ethics in Human Patients Research: The study was conducted in accordance with the Declaration of Helsinki accords for the protection of human patient participants in research.
Conflicts of interest
There are no conflicts of interest.
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