Survival of HIV-infected treatment-naive individuals with documented dates of seroconversion in Rakai, Uganda : AIDS

Secondary Logo

Journal Logo


Survival of HIV-infected treatment-naive individuals with documented dates of seroconversion in Rakai, Uganda

Lutalo, Toma; Gray, Ronald Hb; Wawer, Mariab; Sewankambo, Nelsonc; Serwadda, Davidd; Laeyendecker, Olivere; Kiwanuka, Noaha; Nalugoda, Freda; Kigozi, Godfreya; Ndyanabo, Anthonya; Bwanika, John Baptista; Reynolds, Steven Je; Quinn, Tome; Opendi, Piusa

Author Information
AIDS 21():p S15-S19, November 2007. | DOI: 10.1097/
  • Free



Data on the survival of HIV-infected, treatment-naive individuals in sub-Saharan Africa are needed to provide guidance on the initiation of antiretroviral treatment (ART), to evaluate the effects of treatment on mortality and to project future needs for ART services. Such information is also needed for modeling the epidemic. Studies suggest that survival time from infection to death in sub-Saharan Africa [1–5] may be comparable to those observed in developed countries before the availability of ART [6], but shorter survival times have been reported among commercial sex workers in Kenya [7] and blood donors or military recruits in Thailand [8–10]. Older age is also associated with faster progression [6] and some data suggest that progression might differ by HIV subtype [11–15].

We previously reported on mortality rates by age and HIV status [16]. In this paper we describe survival among individuals with known dates of seroconversion between 1995 and 2003, and explore differentials by sex, age at seroconversion, place of residence, time period of infection and HIV subtype.


Since November 1994, the Rakai Community Cohort Study has conducted annual surveillance in an open cohort of individuals aged 15–49 years, resident in 50 communities in rural Rakai district of southwestern Uganda. The cohort has been described in detail elsewhere [17]. Eligible individuals were identified by annual censuses and consenting participants were administered a sociodemographic and behavioral questionnaire, and provided a venous blood sample for HIV testing. The population under annual surveillance varied from 10 000 to 14 000, because over time the cohort was adapted to specific studies and constrained by available financial resources in some years. The Rakai Health Sciences Program provided health education on HIV prevention, free voluntary HIV counseling and testing, free condoms and syndromic management of sexually transmitted diseases. ART became available in 2004, so analysis of deaths was truncated on 31 December 2003 to assess the survival of ART-naive HIV-infected individuals. Before 2004, the Program was only able to provide treatment for limited opportunistic infections and palliative care.

HIV seroconversion was determined by two separate enzyme immunoassays, with Western blot confirmation. Although the specific enzyme immunoassays and Western blot tests changed over time, the testing algorithm remained constant. In 396 individuals, whose serum samples had sufficient HIV RNA for reverse transcriptase polymerase chain reaction amplification, we were able to identify the viral subtype. These samples were obtained from seroconverters in HIV-discordant couples identified between 1995 and 1998 and for all seroconverters between 1999 and 2002, but costs of the multiple hybridization assay (MHA) precluded subtying of other samples. We determined HIV-1 subtype using MHAacd[18,19]. Subtypes were classified as A, C, D, AD recombinants, and multiple infections. Earlier analyses of Rakai data suggested that subtypes D, AD recombinants and multiple infections have similar disease progression rates [11], and in this study population there were only two individuals with subtype C, so for analysis purposes we compared A with non-A virus subtypes.

The study was approved by four institutional review boards: the Science and Ethics Committee of the Uganda Virus Research Institute (Entebbe, Uganda), the National Council for Science and Technology (Kampala, Uganda), the Committee for Human Research at Johns Hopkins University, Bloomberg School of Public Health (Baltimore, MD, USA), and the Western Institutional Review Board (Olympia, WA, USA).

Statistical analyses

This analysis focuses on initially HIV-negative individuals who seroconverted between 1995 and 2002, and death among seroconverters between 1995 and 2003. The date of seroconversion was defined as the mid-interval between the last negative and the first positive HIV test. Age at seroconversion was calculated from the difference between the seroconversion date and the date of birth, and was categorized into 15–24, 25–29, 30–39 and 40+ year age groups. Residence was stratified into rural communities on secondary roads and urban communities on main roads.

Incident cases with no follow-up information after seroconversion (n = 2) and those with missing date of birth (n = 2) were excluded from this analysis. Individuals who outmigrated were censored at time of exit from the study, and follow-up time was truncated on 31 December 2003 just before ART became available to the population. Death from any cause was considered the outcome event of interest.

All analyses used Stata version 9 (Stata Corp., College Station, Texas, USA). Survival probabilities were estimated using Kaplan–Meier survival methods and the log-rank test was used to test for equality of survival functions between covariates of interest (e.g. sex, age, residence, time period and HIV subtype). Median survival times, interquartile ranges and 95% confidence intervals (CI) were estimated from the Kaplan–Meier analyses [20,21]. If the median survival could not be ascertained using a Kaplan–Meier analysis we used parametric regression to fit a Weibull distribution and extrapolate survival proportions to obtain an estimate of the median survival time and 95% confidence intervals [4]. Both the slope and shape parameters were considered for calculating the 95% confidence intervals for the median time of survival. Cox proportional hazards regression models were used to estimate hazard ratios (HR) of death associated with covariates of interest. Adjusted models included covariates found to be significant at P < 0.15 in univariate analyses, or covariates reported to be associated with survival in other studies.


The distribution of seroconversions and deaths by year are shown in Table 1. The variation in the number of annual seroconversions partly reflects changes in the population under surveillance, as well as trends in HIV incidence. (HIV incidence declined from approximately 2.0 per 100 person-years in 1995–1996 to approximately 1.3 per 100 person-years in 2002, and prevalence declined from 17.7% in 1995 to 12.8% in 2003.) A total of 837 seroconverters were identified and these individuals provided 2330 person-years of observation, with a median follow-up time of 6.7 years (interquartile range 3.9–8.5 years). Of the 837 seroconverters, 480 (57.3%) were women and 357 (42.7%) were men (Table 2). As of data cutoff on 31 December 2003, 122 seroconverters had died (14.6%), with an average annual mortality rate of 5.2 per 100 person-years. The total number of HIV-negative individuals followed was 29 123.

Table 1:
Number of seroconverters, cumulative numbers of individuals at risk, deaths by year and sex plus general characteristics of individuals at risk.
Table 2:
Characteristics of the individuals at risk.

Figure 1 shows the Kaplan–Meier and Weibull survival curves for the whole population. The median time from infection to death could not be ascertained using Kaplan–Meier analysis, but the first quartile (75% survival) was 5.5 years (95% CI 5.0–6.2). The Weibull estimated median survival time was 8.7 years (95% CI 8.1–9.3). There was no difference in survival between men and women (P = 0.19, Fig. 2). As shown in Fig. 2, the probability of survival decreased with older age at infection (P = 0.01). For individuals infected at the age of 40 years or more, the hazard ratio of death was 2.21 (95% CI 1.34–3.65), compared with individuals infected at ages 15–24 years. There was a significant trend towards lower survival with increasing age. There were no differences in survival by place of residence (P = 0.36), or time period of infection (1995–1998 versus 1999–2003, P = 0.7, results not shown).

Fig. 1:
Kaplan–Meier survival curves and Weibull model fit for the whole population of HIV seroconverters.
Table 1
95% Confidence interval;
Table 1
survivor function;
Table 1
Weibull model fit.
Fig. 2:
Kaplan–Meier survival curves by age at infection and by sex. (a) Probability of survival by age group.
Table 1
15–24 years;
Table 1
25–29 years;
Table 1
30–39 years;
Table 1
40+ years. (b) Probability of survival by sex.
Table 1
Table 1

Subtype information was available for 396 individuals, of whom 61 (15.4%) were subtype A, 231 (58.3%) were subtype D, 80 (20.2%) were AD recombinants, 22 (5.6%) were multiple infections (i.e. two or more viral strains) and two (0.5%) were subtype C. For the purpose of analysis we compared subtype A with all non-A subtypes combined, because the latter have similar disease progression rates [11]. As shown in Fig. 3, survival was significantly lower for non-A compared with A infections (log rank P = 0.04). There were only two deaths among the 61 subtype A infections (3.2%), compared with 42 deaths among the combined non-A infections (12.5%), and the unadjusted hazard ratio of death for D and AD versus A infections was 3.88 (95% CI 0.94–16.03, P = 0.06). The median survival time was 7.5 years (95% CI 6.4–8.5) for the combined non-A infections, whereas over 90% of subtype A seroconverters were still alive 7 years after infection, the latest estimate for which direct observations are available.

Fig. 3:
Kaplan–Meier survival curves by A and non-A HIV-1 subtype infections.
Table 1
Subtype A;
Table 1
non-A subtype;
Table 1
unknown subtype.

In a multivariable Cox model, applied to the 396 seroconverters with known viral subtypes, no individual covariates were significantly associated with death, but the adjusted hazards of mortality were of borderline significance for individuals aged 40 years or older at the time of infection (adjusted HR 2.27, 95% CI 0.94–5.48, P = 0.07), and for infections with non-A subtypes (adjusted HR 3.47, 95% CI 0.89–15.44, P = 0.07).


We found a median survival time of 8.7 years from infection to death among a large cohort of seroconverters with known dates of HIV infection. Survival time was reduced among individuals infected at older ages and in those infected with non-A viral subtypes (median survival of 7.5 years).

The median survival time in this rural population is very close to that observed in nearby Masaka district (9.1 years) [22], but is lower than that reported in the rural population of Kisesa, in Tanzania (11.5 years) [4]. Our estimates of survival times are also lower than those for mine workers in South Africa (11.6 years), and for high-income country populations in the pre-ART era [5,6]. The 8.7 year median survival time in Rakai was, however, higher than that observed among commercial sex workers in Kenya [7] and in Thai studies [8–10]. For the non-A subtypes, the 7.5 years (95% CI 6.4–8.5) survival time is less than the lower 95% confidence interval estimated for the other sub-Saharan African countries [1,4,5], and conversely, the upper 95% confidence interval for the non-A subtypes is lower than the point estimates for the other African population. The reasons for this diversity in estimated survival times between African populations is unclear. These differences might be partly the result of methodological considerations or insufficient follow-up time in some studies. We hypothesize that the predominant circulating viral subtypes in different populations might affect the course of HIV disease and thus overall survival. Studies in Uganda [11,13,14], Tanzania [12] and Kenya [15] consistently show faster disease progression with subtype D than subtype A. In Kenya, the median survival time with subtype D was 7.7 years [15], which is comparable to the 7.5 years median survival for non-A subtypes observed in the present study. The divergence of survival estimates between African populations could thus reflect differences in the predominant HIV-1 subtypes, because a higher prevalence of subtype A infections with slower disease progression would increase the estimated survival time for the population as a whole. There are major differences in the epidemiology of circulating HIV-1 subtypes. The Rakai epidemic has a unique predominance of non-A subtypes (84.6%), with only 15.4% subtype A infections. In Masaka, to the north of Rakai, subtype A constituted 36–40% of infections [13,14], in Dar es Salam, Tanzania, 27.3% of infections were subtype A [12], and in Kenya, 78% of infections were caused by subtype A [15]. Moreover, in the Tanzanian and Kenyan studies [12,15], subtypes A and C infections did not differ in rates of disease progression, suggesting that D or AD viruses may be uniquely pathogenic. It has been shown that subtype D and AD recombinant viruses preferentially utilize CXCR4 receptors, which are associated with more advanced disease, whereas subtype A viruses do not demonstrate CXCR4 receptor tropism [11]. There is a great diversity of viral subtypes in sub-Saharan Africa, with predominant C epidemics in southern Africa, A and D epidemics in east–central Africa and multiple subtypes in west Africa [23,24]. Survival from infection to death may thus vary between African populations, possibly depending on the predominant viral strains in each setting. In addition, differential viral subtype pathogenicity might also account for the shorter survival times in Thailand, where most infections are subtype E, which is also thought to be more pathogenic than other viruses [25].

In summary, we found lower survival times in rural Rakai than in other African and European populations. Non-A subtypes appear to have lower survival times compared with subtype A viruses, and it is possible that median survival time estimates differ depending on the predominant viral subtype in the population.


The authors thank the Rakai Health Sciences Program staff for their exceptional work, Dr Edward Katongole-Mbidde, Director, Uganda Virus Research Institute, for his support, Basia Żaba (LSHTM), Jim Todd (MRC) and the ALPHA network for their guidance and the people of Rakai for their generous cooperation

Sponsorship: The Rakai study was funded through the Department of the Army, United States Army Medical Research and Material Command Cooperative Agreement DAMD17-98-2-8007; grants R01 A134826 and R01 A134265 from the National Institute of Allergy and Infectious Diseases; grant 5P30HD06826 from the National Institute of Child and Health Development; the World Bank STI Project, Uganda; the Henry M. Jackson Foundation; a grant 5D43TW00010 from the Fogarty Foundation; the Division of Intramural Research, NIAID, NIH and the Bill and Melinda Gates Institute for Population and Reproductive Health at Johns Hopkins University.

Conflicts of interest: None.


1. Morgan D, Mahe C, Mayanja B, Okongo JM, Lubega R, Whitwort JA. HIV infection in rural Africa: is there a difference in median time to AIDS and survival compared with that in industrialized countries? AIDS 2002; 16:597–603.
2. Crampin AC, Floyd S, Glynn JR, Sibande F, Mulawa D, Nyondo A, et al. Long term follow-up of HIV-positive and HIV-negative individuals in rural Malawi. AIDS 2002; 16:1545–1550.
3. Leroy V, Msellati P, Lepage P, Batungwanayo J, Hitimana DG, Taelman H, et al. Four years of natural history of HIV-1 infection in African women: a prospective cohort study in Kigali (Rwanda), 1988-1993. J Acquir Immune Defic Syndr 1995; 9:415–421.
4. Isingo R, Zaba B, Marston M, Ndege M, Mngara J, Mwita W, et al. Survival after HIV infection in the pre-antiretroviral therapy era in a rural Tanzanian cohort. AIDS 2007; 21(Suppl. 6):S5–S13.
5. Glynn JR, Sonnenberg P, Nelson G, Bester A, Shearer S, Murray J. Survival from HIV-1 seroconversion in Southern Africa: a retrospective cohort study in nearly 2000 gold miners over 10 years of follow up. AIDS 2007; 21:625–632.
6. [Collaborative Group on AIDS Incubation and HIV Survival Including the CASCADE EU Concerted Action. Time from HIV-1 sero-conversion to AIDS and death before the widespread use of highly-active antiretroviral therapy: a collaborative re-analysis.Lancet 2000; 355:1131–1137.
7. Anzala OA, Nagelkerke NJ, Bwayo JJ, Holton D, Moses S, Ngugi EN. Rapid progression to disease in African sex workers with human immunodeficiency virus type 1 infection. J Infect Dis 1995; 171:686–689.
8. Rangsin R, Chui J, Khamboonruang C, Sirisopana N, Eiumtrakul S, Brown AE. The natural history of HIV-1 infection in young Thai men after seroconversion. J Acquir Immune Defic Syndr 2004; 36:622–629.
9. Kilmarx PH, Limpakarnjanarat K, Saisorn S, Mock PA, Mastro TD. High mortality among women with HIV-1 infection in Thailand. Lancet 2000; 356:770–771.
10. Nelson KE, Costello C, Suriyanon V, Sennun S, Duerr A. Survival of blood donors and their spouses with HIV-1 subtype E (CRF01 A_E) infection in northern Thailand, 1992–2007. AIDS 2007; 21(Suppl. 6):S47–S54.
11. Laeyendecker O, Li X, Arroyo MA, McCutchan F, Gray R, Wawer M, et al.The effect of HIV subtype on rapid disease progression in Rakai. In: 13th Conference on Retroviruses and Opportunistic Infections. Denver, CO, 5–8 February 2006 [Abstract 44LB].
12. Vasan A, Renjifo B, Hertzmark E, Chaplin B, Msamanga G, Esseb M, et al. Different rates of disease progression of HIV type 1 infection in Tanzania based on infecting subtype. Clin Infect Dis 2006; 42:843–852.
13. Kaleebu P, Ross A, Morgan D, Yirrel D, Oram J, Rutebemberwa A, et al. Relationship between HIV-1 Env subtypes A and D and disease progression in a rural Ugandan cohort. AIDS 2001; 15:293–299.
14. Senkaali D, Muwonge R, Morgan D, Yirrel D, Whitworth J, Kaleebu P. The relationship between HIV type 1 disease progression and V3 serotype in a rural Ugandan cohort. AIDS Res Hum Retroviruses 2004; 20:932–937.
15. Baeten JM, Chohan B, Lavreys L, Chohan V, McClelland RS, Certain L, et al. HIV-1 subtype D infection is associated with faster disease progression than subtype A in spite of similar plasma HIV-1 loads. J Infect Dis 2007; 195:1177–1180.
16. Sewankambo NK, Gray RH, Ahmad S, Serwadda D, Wabwire-Mangen F, Nalugoda F, et al. Mortality associated with HIV infection in rural Rakai District, Uganda. AIDS 2000; 14:2391–2400.
17. Wawer MJ, Gray RH, Sewankambo NK, Serwadda D, Paxton L, Berkley S, et al. A randomized, community trial of intensive sexually transmitted disease control for AIDS prevention, Rakai, Uganda. AIDS 1998; 12:1211–1225.
18. Arroyo MA, Hoelscher M, Sanders-Buell E, Herbinger KH, Samky E, Maboko L, et al. HIV type 1 subtypes among blood donors in the Mbeya region of southwest Tanzania. AIDS Res Hum Retroviruses 2004; 20:895–901.
19. Hoelscher M, Dowling WE, Sanders-Buell E, Carr JK, Harris M, Thomschke A, et al. Detection of HIV-1 subtypes, recombinants, and dual infections in east Africa by a multi-region hybridization assay. AIDS 2002; 16:2055–2064.
20. Stata Corp. Stata survival analyses and epidemiological tables. Stata statistical software: release 9. College Station, TX: StataCorp LP; 2005.
21. Klein JP, Moeschberger ML. Survival analysis: techniques for censored and truncated data, 2nd ed. New York: Springer; 2003. pp. 167–168.
22. Van der Paal L, Shafer LA, Todd J, Mayanja BN, Whitworth JAG, Grosskurth H. HIV-1 disease progression and mortality before the introduction of highly active antiretroviral therapy in rural Uganda. AIDS 2007; 21(Suppl. 6):S21–S29.
23. Peeters M, Toure-Kane C, Nkengasong JN. Genetic diversity of HIV in Africa: impact on diagnosis, treatment, vaccine development and trials. AIDS 2003; 17:2547–2560.
24. Hu D, Vanischseni S, Mastro T, Raktham S, Young NL, Mock PA, et al. Viral load differences in early infection with two HIV-1 subtypes. AIDS 2001; 15:683–691.
25. McCutchan FE. Global epidemiology of HIV. J Med Virol 2006; 78(Suppl. 1):S7–S12.

HIV survival; subtype; Uganda

© 2007 Lippincott Williams & Wilkins, Inc.