Prompt initiation of antiretroviral therapy (ART) for HIV-infected infants is strongly recommended but diagnostic confirmation is important as committing children to life-long ART carries serious health and social implications.
Two HIV-exposed infants in Johannesburg, South Africa were identified presenting with unusual trajectories of diagnostic nucleic acid amplification tests (NAAT) and viral load results.
Case 1 had repeat indeterminate NAAT results during the first 3 weeks of life; repeat testing thereafter was negative with undetectable viral load including after daily nevirapine prophylaxis ended. ART was not initiated at this time. Case 2 had a single positive NAAT result at 1 month of age that prompted initiation of ART. Subsequent results were negative and ART was discontinued. Repeat negative NAAT with viral load below the limit of quantification or undetectable continued to be obtained. Shortly after and around weaning, positive NAAT results with high viral load (7.1 and 6.03 log10 copies/ml for Cases 1 and 2, respectively) were observed in both children. Both mothers were treated with tenofovir, emtricitabine and efavirenz during breastfeeding. Testing with ultrasensitive assays on early samples conclusively revealed HIV-1 proviral DNA in Case 1. Testing with ultrasensitive assays after the early period but prior to weaning did not detect HIV in either infant.
We hypothesize that breast milk from the mothers of these two rare cases had HIV-specific or nonspecific factors that led to the undetectable results in already infected infants until breastfeeding ended. Our results raise the importance of repeat testing of HIV-exposed breast-fed infants after complete cessation of all breastfeeding.
aEmpilweni Services and Research Unit, Rahima Moosa Mother and Child Hospital, Department of Paediatrics and Child Health, Faculty of Health Sciences, University of the Witwatersrand, Rahima Moosa Mother and Child Hospital
bCentre for HIV and STIs, National Institute for Communicable Diseases, National Health Laboratory Services
cFaculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
dGertrude H. Sergievsky Center, College of Physicians and Surgeons
eDepartment of Epidemiology
fICAP at Columbia University, Mailman School of Public Health
gDepartment of Pediatrics, Vagelos College of Physicians & Surgeons, Columbia University, New York, New York, USA
hDepartment of Medical Virology, Faculty of Health Sciences, University of Pretoria, Pretoria, South Africa.
Correspondence to Louise Kuhn, PhD, Gertrude H. Sergievsky Center, College of Physicians and Surgeons, Columbia University, 630 W 168th Street, New York, NY 10032, USA. Tel: +1 212 3052398; fax: +1 212 3052424; e-mail: firstname.lastname@example.org
Received 6 February, 2019
Revised 30 April, 2019
Accepted 17 May, 2019
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