D-26 Free Communication/Poster - Carbohydrate Metabolism in Health/Disease Thursday, June 2, 2016, 1: 00 PM - 6: 00 PM Room: Exhibit Hall A/B
High-intensity interval training (HIT) represents a very time-efficient mode of exercise training and induces several health benefits. However, little is known regarding the role of HIT to combat whole body insulin resistance.
PURPOSE: To investigate the effect of HIT on whole body insulin resistance in high-fat diet (HFD)-induced obese mice.
METHODS: At 5-week postnatal period, a total of 30 male mice (C57BL/6) were randomly assigned to standard chow (SC) (n=10) or HFD (n=20) for 23 weeks. After 15 weeks of dietary treatment, the HFD mice were further assigned to HFD (n=10) or HFD+HIT (HFD+HIT, n=10). The HFD+HIT mice were subjected to HIT on a motor-driven rodent treadmill during the last 8 weeks of the 23-week HFD course.
RESULTS: HIT suppressed HFD-induced increases in body weight (HFD 47.3±1.4 vs. HFD+HIT 44.2±1.2g, p=0.023), subcutaneous fat mass (HFD 2.7±0.1 vs. HFD+HIT 2.4±0.1g, p=0.038), serum total cholesterol (HFD 253.5±48.5 vs. HFD+HIT 198.7±7.4mg/, p=0.010), area under the curve of glucose tolerance test (HFD 53070±8585 vs. HFD+ HIT 45401±4312, p=0.031), and area under the curve of insulin tolerance test (HFD 9367±987 vs. HFD+ HIT 6416±619, p=0.016). HIT prevented HFD-induced decreases of total adiponectin in serum (p=0.001) and adipose tissue (p=0.016). Along with improved metabolic risk factors, HIT prevented HFD-induced decreases in proteins of adiponectin receptor 1 (p=0.010), AMP-activated protein kinase (p=0.001), and NAD-dependent deacetylase sirtuin-1 (p=0.010) and HFD-induced decreases in mRNAs of peroxisome proliferator-activated receptor- (p=0.027), carnitine palmitoyltransferase I (p=0.023), and acyl CoA oxidase (p=0.030) in skeletal muscle.
CONCLUSION: The current findings show that HIT alleviates whole body insulin resistance associated with obesity via the AdipoR1 and AMPK mediated-signaling pathway in skeletal muscle, implying the therapeutic role of HIT to combat whole body insulin resistance.
Supported by the National Research Foundation Grant funded by the Korean Government (NRF-2015S1A5B5A02012775).