D-32 Free Communication/Poster - Fat Metabolism I Thursday, May 28, 2015, 1: 00 PM - 6: 00 PM Room: Exhibit Hall F
The effects of exercise on the gastrointestinal (GI) tract are considerable. However, the metabolic fluctuations within the GI tract after acute exercise are largely understudied. Short chain fatty acids (SCFAs) are the major metabolic end-products of bacterial mediated fermentation and have pleiotropic effects on GI epithelial cells, barrier integrity, satiety hormones, and the immune system. We hypothesized that exercise-induced lactate production would increase SCFAs in the ceca of mice.
PURPOSE: To determine whether acute moderate or high intensity exercise bouts alter SCFA concentrations within the GI tracts of mice.
METHODS: BALB/C mice were randomized into 3 groups: sedentary (SED, n=4), moderate intensity running (MOD, n=5) and high intensity running (HI, n=5). The MOD and HI group underwent forced treadmill running for 40 minutes at 12 m/min or 7 minutes at 28–30 m/min, respectively. The SED group remained in home cages. Immediately after exercise, blood was drawn through tail snip and blood lactate was determined. Forty-five minutes after exercise, mice were sacrificed by CO2 asphyxiation. The ceca were dissected longitudinally, and colon contents were removed and assessed by percentage of dry matter weight via gas chromatography–mass spectrometry (GC-MS).
RESULTS: Acute, high intensity exercise (HI) increased the levels of three major SCFAs, acetate (AC), propionate (PRO) and butyrate (BU), compared to non-exercised mice (SED) (p<0.05). Moderate intensity exercise (MOD) increased PRO, but not AC or BU, compared to SED (p<0.05). Interestingly, the HI exercise group tended to increase BU compared to the MOD exercise group (p=0.06).
CONCLUSIONS: Acute exercise bouts increase the levels of SCFAs in the ceca of mice 45 minutes after the cessation of exercise. The degree of these fluctuations may be dependent on the intensity of the exercise.