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A Single Bout of Premeal Resistance Exercise Improves Postprandial Glucose Metabolism in Obese Men with Prediabetes

BITTEL, ADAM J.1; BITTEL, DANIEL C.1; MITTENDORFER, BETTINA2; PATTERSON, BRUCE W.2; OKUNADE, ADEWOLE L.2; ABUMRAD, NADA A.2; REEDS, DOMINIC N.2; CADE, WILLIAM TODD1,3

Author Information
Medicine & Science in Sports & Exercise: April 2021 - Volume 53 - Issue 4 - p 694-703
doi: 10.1249/MSS.0000000000002538

Abstract

Prediabetes is a metabolic condition defined by elevated fasting (impaired fasting glucose [IFG]) and/or postprandial (impaired glucose tolerance [IGT]) plasma glucose (1). Prediabetes affects nearly 86 million adults in the United States, with most (up to 70%) ultimately progressing to type 2 diabetes (T2D) within as little as 1 yr (2). Although evidence suggests that T2D can be reversed, most cases of remission involve invasive treatment strategies, including bariatric surgery to promote weight loss, or extreme lifestyle changes (e.g., severe caloric restriction) that have poor long-term adherence (3,4). Therefore, preventing the initial progression of prediabetes to T2D would have profound health implications globally.

Previous studies have shown that lifestyle interventions combining diet and exercise can reverse prediabetes and restore normoglycemia. In 2002, the Diabetes Prevention Program Research Study demonstrated that lifestyle interventions aiming for 7% weight loss and 150 min·wk−1 of moderate-intensity endurance type physical activity reduced the incidence of T2D by 58% and was significantly more effective than treatment with metformin alone in individuals with prediabetes (5). Likewise, the Finnish Diabetes Prevention Study found that a lifestyle intervention focusing on weight loss, reduced total fat intake, and physical activity (combined moderate aerobic and resistance exercise [RE]) reduced the incidence of T2D by 58% in obese men and women with IGT (6). In light of these large-scale studies, the American Diabetes Association currently recommends individuals with prediabetes improve their diet and increase time spent engaged in moderate- to high-intensity physical activities (goal: 150 min·wk−1 of physical activity) to prevent or delay the onset of T2D (7).

The effect of RE, which is a popular alternative to endurance type physical activities, on glucose metabolism and the risk of T2D is understudied, and the results are equivocal (evidence grade B or C) (7). For example, in healthy, young, nonobese participants, RE reduced plasma glucose and insulin responses to an oral glucose tolerance test because of increased glucose clearance (8–10). In older, overweight subjects with prediabetes, 12 wk of RE improved glucose tolerance, but not insulin sensitivity, during an oral glucose tolerance test (11). Although these studies provide preliminary support for the benefits of RE on postprandial glucose metabolism, the use of oral glucose tolerance testing and the study of glucose concentrations alone provides limited insight into mixed meal glucose responses. It is currently unknown if RE reduces postprandial glucose appearance or increases glucose clearance (including glucose oxidation), improves beta cell function, increases insulin sensitivity, or augments hepatic insulin sensitivity after a mixed meal in individuals with prediabetes—mechanisms that would reduce postprandial hyperglycemia and reduce the risk for progression to T2D. Understanding the effects of RE on mixed meal responses is critical to enhance exercise recommendations for these individuals because 1) mixed meal ingestion reflects free-living conditions (most meals consist of carbohydrates, proteins, and lipids), 2) most of the day is spent in the postprandial state, and 3) postprandial hyperglycemia is a major independent risk factor for cardiovascular disease (CVD) and other serious comorbidities (e.g., hypertension and chronic kidney disease) (12–14).

Therefore, the purpose of the present study was to determine the effects of a single bout of RE on postprandial glucose metabolism after a mixed meal in obese, sedentary men with prediabetes. We used a stable isotopically labeled glucose tracer infusion in conjunction with a mixed meal to measure basal and postprandial glucose rate of appearance (Ra) into and rate of disappearance (Rd) from plasma, insulin sensitivity (assessed as postprandial glucose Rd area under the curve divided by plasma insulin area under the curve), and the insulinogenic index (a measure of beta cell function). We also obtained muscle biopsies to evaluate pyruvate-supported mitochondrial respiration and assessed postprandial whole-body substrate oxidation by using a metabolic cart. We hypothesized that a single bout of RE would reduce the glycemic response to a mixed meal by increasing glucose clearance rates, insulin sensitivity, and carbohydrate oxidation.

METHODS

The participants and the experimental protocol used in this study have been described in our previous publication that focused on the effects of RE on postprandial lipid metabolism (15). Here we provide additional information on the methods used to study glucose metabolism in these same participants.

Participants

Participants were recruited from the Washington University in St. Louis (WUSTL) School of Medicine Diabetes Clinic, the WUSTL Volunteers for Health, the Center for Community Based Research databases, and advertising in the St. Louis metropolitan area from August 2015 to May 2018. Potential participants completed a detailed screening evaluation, including a history and physical examination, serum blood chemistries (HbA1c, a lipid panel, a comprehensive metabolic panel, and a complete blood count), and an oral glucose tolerance test [for further details, see Appendix, Supplemental Digital Content, Electronic Supplementary Materials (ESM), https://links.lww.com/MSS/C185].

Participants were included if they were male, 30–65 yr old with a body mass index of 28–45 kg·m−2, and met the criteria for prediabetes (HbA1c >38 but <48 mmol·mol−1 [>5.7 but <6.5%]), fasting plasma glucose >5.6 mmol·L−1 but <7.0 mmol·L−1, or 2-h OGTT glucose >7.8 mmol·L−1 but <11.1 mmol·L−1) at 120 min (16). Only men were selected to avoid the confounding effects of sex-based differences in fasted and postprandial glucose metabolism, including stronger predisposition for IGT in men, superior postprandial glucose uptake in adipose tissue and skeletal muscle in women, increased use of carbohydrate relative to fat metabolism in the fasted and postprandial states in women, and preferential sparing of lipids during exercise recovery in women compared with men (17,18). Participants were excluded if they were diagnosed with T2D, used insulin or other blood sugar–lowering medications, participated in regular exercise (≥2 times per week) within the previous 6 months (assessed by questionnaire during the initial phone interview), and had a history of pulmonary or cardiovascular disease, coagulation disorders, anemia, or orthopedic, neurologic, metabolic, or other medical condition that would prohibit them from participating in the exercise and metabolic testing protocol. Participants also completed a one-repetition maximum (1RM) strength assessment, daily physical activity monitoring, and body composition measurements by using dual-energy x-ray absorptiometry [for further details, see Appendix, Supplemental Digital Content, Electronic Supplementary Materials (ESM), https://links.lww.com/MSS/C185]. The study was approved by the WUSTL Human Research Protection Office (protocol no. 20160495), and all participants provided written informed consent before enrollment. All procedures were completed in accordance with the ethical principles outlined in the Declaration of Helsinki. Participant characteristics are shown in Table 1.

TABLE 1 - Participant characteristics.
Measure Mean ± SEM
Age (yr) 50 ± 9
Height (cm) 178 ± 6
Weight (kg) 105 ± 12
Body mass index (kg·m−2) 33 ± 3
Fat mass (kg) 33 ± 6.4
Lean mass (kg) 67 ± 6.2
% Body fat 31.6 ± 4
HbA1c (%) 5.7 ± 0.5
HOMA2-%B 139.4 ± 56
HOMA2-%S 46 ± 16
Fasting glucose (mmol·L−1) 5.9 ± 0.7
2-h OGTT glucose (mmol·L−1) 9.1 ± 0.5
Fasting insulin (pmol·L−1) 131 ± 57
Triglycerides (mmol·L−1) 1.9 ± 0.27
Total cholesterol (mmol·L−1) 4.8 ± 0.83
HDL cholesterol (mmol·L−1) 0.99 ± 0.13
LDL cholesterol (mmol·L−1) 3.0 ± 0.75
% Time sedentary 69 ± 19.2
% Time light activity 21 ± 11.4
% Time moderate activity 10 ± 9.5
% Time vigorous activity 0 ± 0
% of time spent in each activity was derived from accelerometry data (see ESM). HOMA2 is calculated using the HOMA2 calculator (http://www.dtu.oc.ac.uk) (19).
2-h OGTT, plasma glucose at 2-h after a 75-g oral glucose challenge.

Study Protocol

Glucose metabolism study

Each participant completed two glucose metabolism studies (exercise and rest) in randomized order, approximately 1 wk apart (Fig. 1). Treatment order (rest or exercise) was assigned through simple random sampling without replacement (see Appendix, Supplemental Digital Content, Electronic Supplementary Materials (ESM), https://links.lww.com/MSS/C185). Between studies, participants were instructed to maintain their normal diet and daily activity level, which was verbally confirmed before beginning the second metabolism study. During the exercise study, participants completed a bout of whole-body RE 270 min before meal ingestion; for the rest study, participants rested in a chair.

F1
FIGURE 1:
Study protocol.

The day before each glucose metabolism study, participants were provided standardized breakfast (2.18 MJ), lunch (3.02 MJ), afternoon snack (0.75 MJ), and dinner (3.26 MJ) from the WUSTL Clinical and Translational Research Unit (CTRU) Bionutrition Service. They consumed a liquid formula (Ensure; Ross Laboratories, Columbus, OH) containing 1.05 MJ at ~2200 h to ensure complete filling of hepatic glycogen stores. The carbohydrate, fat, and protein contents for each meal are provided in the ESM (see Appendix, Supplemental Digital Content, Electronic Supplementary Materials (ESM), https://links.lww.com/MSS/C185). Participants then fasted overnight (10 h) except for water and reported to the CTRU at 0730 h the next morning. After admission, an intravenous catheter was inserted into the antecubital vein to collect baseline blood samples and later for tracer infusions.

During the rest study, participants rested quietly in the semirecumbent position for 60 min from 0900 h to 1000 h. During the exercise study, participants performed 1 h (from 0900 h to 1000 h) of RE consisting of 3 sets of 10–12 reps at 80% 1RM of the following seven exercises: leg press, knee extension, chest press, shoulder press, seated row, pull down, and biceps curl (see Appendix, Supplemental Digital Content, Electronic Supplementary Materials (ESM), https://links.lww.com/MSS/C185).

After the rest or the RE period for each study, a second catheter was inserted into a hand vein on the contralateral arm; the hand was warmed with a warming box (55°C) to collect arterialized blood samples. To allow time for catheter insertion, the study timer was started (study time 0) 30 min postexercise or postrest. At 0 min, a primed, constant infusion of [6,6-2H2]glucose (28 μmol·kg−1 prime and 0.28 μmol·kg−1⋅min−1 continuous infusion; Cambridge Isotope Laboratories, Tewksbury, MA) was initiated and continued to the end of the study. At 180 min, participants were given a liquid meal (Boost Plus) consisting of 0.08 MJ·kg−1 fat-free mass (FFM) (47.8% carbohydrate, 36.1% fat, and 16.1% protein). Blood samples were collected at −10, 80, 160, 170, 180, 196, 210, 215, 225, 240, 270, 300, 330, 360, 420, 480, and 540 min (Fig. 1).

At 270 min (90-min postmeal), biopsy samples from the vastus lateralis were obtained. We selected 90 min to allow adequate time for meal digestion, hormone secretion, and glucose uptake before probing for changes mitochondrial function, as well as to provide time for procedural setup. Whole-body carbohydrate oxidation rate was measured at baseline (−90 min), immediately before the RE/rest period, and at 140, 210, 240, 330, 390, 450, and 510 min using indirect calorimetry as previously described (17). The time to peak carbohydrate oxidation was measured for each participant.

Sample processing

Blood for plasma hormone and metabolite analysis was collected from an antecubital vein, immediately placed in chilled EDTA tubes, centrifuged at 2000g for 10 min, and the supernatant collected and frozen at −80°C until analysis. The tracer-to-tracee ratio (TTR) for plasma [2H2]glucose was quantified using capillary GC/MS (Agilent 6890 N gas chromatograph and Agilent 5973 N mass selective detector; Agilent, Palo Alto, CA), as previously described (20). Plasma insulin concentration was measured using electrochemiluminescence immunoassay, and plasma glucose concentration was measured using the glucose oxidase method (Yellow Springs, OH).

Tissue Biopsies and Mitochondrial Respiration

Biopsies from the vastus lateralis were collected under local anesthesia as previously described (19,21). Skeletal muscle (6–10 mg) was used for mitochondrial high-resolution respirometry analysis (Oxygraph-2 k; Oroboros Instruments, Innsbruck, Austria; see Appendix, Supplemental Digital Content, Electronic Supplementary Materials, methods and ESM Table 1, https://links.lww.com/MSS/C185). Remaining muscle and adipose tissue samples were irrigated with saline, flash frozen in liquid nitrogen, and stored at −80°C until analysis. Pyruvate-supported respiration was calculated as follows:

MaxOXPHOSETF+CI+CIILip+PyrFFAoxidationETF+CILip

(see Appendix, Supplemental Digital Content, Electronic Supplementary Materials (ESM), https://links.lww.com/MSS/C185).

Calculations

Indices of beta cell function (HOMA-%B) and insulin sensitivity (HOMA-%S) were calculated from fasting glucose and insulin values before the mixed meal test using the Homeostatic Model Assessment 2 (HOMA2) calculator (22). Basal glucose Ra was calculated by dividing the [6,6-2H2]glucose infusion rate (μmol·min−1) by the plasma tracer enrichment (mean of all three measurements before the start of the mixed meal test at time 160, 170, and 180 min) (20). Non–steady-state glucose kinetics during the mixed meal were assessed as previously described using plasma glucose concentration and 2H2-glucose TTR values to assess the total Ra of glucose into plasma (sum of oral glucose appearance + endogenous glucose production [EGP]) and total rate of glucose Rd (23). The postprandial [2H2]-glucose TTR for RE and rest is shown in ESM Figure 1 (see Appendix, Supplemental Digital Content, Electronic Supplementary Materials (ESM), https://links.lww.com/MSS/C185). Ra and Rd were normalized per kilogram of FFM from dual-energy x-ray absorptiometry.

The total area under the curve (AUC) was calculated using the trapezoid rule; incremental AUC (iAUC) were obtained after adjusting for basal values (24). Overall postprandial insulin sensitivity was calculated as the glucose Rd AUC/insulin AUC (25). The postprandial insulinogenic index was calculated as the ratio of the change in insulin concentration to the change in glucose concentration over the first 30 min of the postprandial period (ΔI0–30/ΔG0–30) (26).

Statistical Analysis

Differences in glucose and insulin concentrations, carbohydrate oxidation, and glucose Ra and Rd time courses between rest and exercise were assessed by using repeated-measures ANOVA with treatment (rest/RE) and time (before/after meal) as repeated factors, and order (rest > RE or RE > rest) as a between-subject factor. Significant interactions between treatment and time were further analyzed using paired t-tests. The Benjamini–Hochberg procedure was used to control for multiple comparisons for all repeated measures. AUC, basal glucose Ra and Rd, insulinogenic index, insulin sensitivity, time to peak carbohydrate oxidation, and mitochondrial respirometry measures were analyzed using paired t-tests. An alpha level of 0.05 was used for significance. All analyses were completed using the Statistical Package for the Social Sciences version 25. A conservative effect size of 1 was used to sample size calculations based on the results from Breen et al. (8), who reported an effect size of 2 for an increase in glucose Rd after a single session of RE in healthy men. Therefore, a sample size of 10 was required to achieve 80% power to detect differences on a paired t-test for glucose disposal (at a two-tailed alpha level of 0.05).

RESULTS

Participant Demographics

Ten (n = 10) participants were enrolled in the study. Participants were 39–62 yr of age, obese, and demonstrated insulin resistance with compensatory increases in beta cell function. Participants also presented with elevated mean total triglyceride, LDL, and low HDL concentrations and spent the majority of their day in sedentary activity (Table 1). Six participants had both IFG and IGT, two had IGT alone, one participant had IFG alone, and one participant qualified based on their HbA1c >5.7%.

Plasma Glucose and Insulin Concentrations

Compared with rest, the plasma glucose concentration was significantly elevated immediately after exercise (time −10 min in Fig. 2A, P = 0.005). After meal consumption, plasma glucose concentration and glucose AUC were not different between conditions (Fig. 2A and B). However, the postprandial glucose iAUC was significantly lower after RE than rest (180–540 min, P = 0.015), indicating a reduced glycemic response to the mixed meal when normalized to premeal (measured at 180 min) glucose concentration (Fig. 2C).

F2
FIGURE 2:
Plasma glucose and insulin concentrations measured throughout the study (mean ± SEM). Time point “0” corresponds to the start of the [6,6-2H2]glucose tracer. A, Plasma glucose (mmol·L−1). B and C, Postprandial total and incremental area under the curve (AUC and iAUC, respectively) for plasma glucose concentration. D, Plasma insulin (pmol·L−1). *Significant elevation in plasma glucose at time −10 min in RE condition (paired t-test), and significant main effect of treatment (RE < rest) for postprandial insulin concentration (repeated-measures ANOVA, see text for P values). E and F, Postprandial total and incremental (AUC and iAUC, respectively) for plasma insulin. *Significant difference between rest and exercise (paired t-test, P < 0.05) in panels B, C, E, and F.

Like plasma glucose, plasma insulin was significantly elevated immediately after the RE session compared with rest (Fig. 2D, −10 min, P = 0.02) and returned to baseline by the end of the basal period before the meal. In the postprandial period, there was a main effect of time (P < 0.001) and a main effect of treatment (P = 0.041) for plasma insulin concentration, with total plasma insulin significantly reduced after exercise compared with rest (Fig. 2B). The plasma insulin AUC and iAUC were also significantly reduced in the RE condition compared with rest (P = 0.020 and P = 0.038, respectively; Fig. 2E and F).

Plasma Glucose Kinetics

Basal glucose Ra in plasma (an index of hepatic glucose production) was significantly greater after RE compared with rest (Table 2). In the postprandial period, there was no significant effect of RE on postprandial total glucose Ra (which represents the sum of EGP and meal glucose appearance in plasma) or Rd (Fig. 3A and D). However, both the meal-induced rise in glucose Ra and Rd (Ra iAUC and Rd iAUC) were significantly reduced in the RE condition compared with rest (P = 0.002 for both measures; Fig. 3C and F).

TABLE 2 - Basal (fasted) and postprandial glucose kinetics, total carbohydrate oxidation.
Rest RE P
Basal glucose Ra (μmol·kg−1 FFM⋅min−1) 13.3 ± 0.46 15.5 ± 0.72 0.02
Postprandial insulin sensitivity (Rd AUC/insulin AUC) (μmol·L−1/[pmol·kg−1 FFM⋅min−1]) 0.043 ± 0.003 0.055 ± 0.007 0.02
Insulinogenic index (ΔI0–30/ΔG0–30) 291 ± 46 312 ± 50 0.77
Carbohydrate oxidation iAUC
([g·kg−1 FFM⋅min−1] × 360 min)
0.18 ± 0.1 0.20 ± 0.1 0.76
Time of peak carbohydrate oxidation (min from start of meal) 144 ± 24 219 ± 26 0.04
Values are presented as mean ± SEM.
AUC, area under the curve determined using the trapezoid rule; I, insulin; G, glucose.

F3
FIGURE 3:
Postprandial glucose kinetics and insulin sensitivity. All values are presented as mean ± SEM. A, Postprandial glucose Ra. B and C, Postprandial total and incremental area under the curve (AUC and iAUC, respectively) for glucose Ra. D, Postprandial glucose Rd. E and F, Postprandial total and incremental area under the curve (AUC and iAUC, respectively) for glucose Rd. *Significant difference between rest and exercise (paired t-test, P < 0.05) in all graphs.

Insulin Sensitivity and Beta Cell Function

In the postprandial period, whole-body insulin sensitivity, assessed as glucose Rd AUC/insulin concentration AUC, was significantly higher after RE, indicating a greater rate of glucose clearance per unit of insulin (Table 2). The insulinogenic index, which assesses the early postprandial rise in plasma insulin in relation to the rise in plasma glucose, was not significantly different after the RE session compared with rest (Table 2).

Carbohydrate Oxidation and Mitochondrial Respiration

There was a main effect of time (P < 0.001) (i.e., a meal effect) for carbohydrate oxidation rate (g·kg−1 FFM⋅min−1), but no effect of RE (Fig. 4A). Likewise, total postprandial carbohydrate oxidation AUC was not significantly elevated in the postprandial period after RE compared with rest (Table 2). The time course analysis showed that the peak of carbohydrate oxidation occurred significantly later in the postprandial period after RE compared with rest (Table 2 and Fig. 4A). Skeletal muscle mitochondrial pyruvate-supported respiration tended to be higher (P = 0.061), but the contribution of pyruvate-supported respiration contribution to maximal oxidative respiration was significantly lower (P = 0.034), after RE compared with rest (Fig. 4B and C).

F4
FIGURE 4:
Carbohydrate oxidation and mitochondrial respiration. All values are presented as mean ± SEM. A, Total carbohydrate oxidation (g·kg−1 FFM⋅min−1) determined using indirect calorimetry. B, Pyruvate-supported mitochondrial respiration. C, Pyruvate-supported respiration normalized to maximal oxidative phosphorylation. *Significant difference between rest and exercise (paired t-test, P < 0.05). †Main effect of time for carbohydrate oxidation (P < 0.001).

DISCUSSION

The effects of an acute bout of RE on postprandial glucose metabolism in individuals with prediabetes are poorly understood. Previous studies have reported that postmeal RE reduces postprandial glucose excursions in healthy individuals and in those with T2D (27). To our knowledge, only one study has evaluated the effect of RE on postprandial (OGTT) glucose metabolism in prediabetes, which effectively increased glucose tolerance (11). We have expanded on this initial study to show the novel finding that a single bout of RE performed 4.5 h before a mixed meal (as opposed to an OGTT) reduced total postprandial glucose appearance (Ra iAUC), increased insulin sensitivity, and reduced the glycemic response to a mixed meal, but had no effect on glucose oxidation in obese men with prediabetes. Improvements in insulin sensitivity were complemented by reduced postprandial insulin concentration, further highlighting the beneficial effects of RE on postprandial glucoregulation.

Previous studies have found that the contribution of postprandial glycemia to plasma HbA1c increases as the degree of glycemic control decreases in healthy individuals with HbA1c between 6.0% and 7.0% (28). Therefore, in individuals with prediabetes who have only modestly impaired glycemic control, reducing the degree of postprandial glycemia could be an integral component of their treatment. We found that RE effectively minimized the rise in plasma glucose from basal values (glucose iAUC) compared with rest, which was likely due to a reduction in the rise in postprandial glucose Ra above the basal rate (Ra iAUC). Because we did not use a meal glucose tracer, we were unable to delineate between endogenous and exogenous (meal) glucose kinetics. After meal ingestion, the liver is preferentially and immediately exposed to rising insulin concentrations through the portal vein, which serves to rapidly suppress EGP and promote glucose uptake (estimated to account for 30%–40% of postprandial glucose clearance) (29). In healthy individuals, physiological postprandial insulin concentrations are sufficient to suppress EGP by ~55% (29). The preferential exposure of the liver to hyperinsulinemia may be a protective mechanism to avoid the negative consequences of increased arterial insulin concentrations (e.g., coagulation abnormalities and atherosclerosis), with skeletal muscle glucose serving as a backup system for glucose clearance (accounting for an additional 30%–40% of postprandial glucose Rd) (29). The reduction in postprandial glucose Ra iAUC may be due to increased hepatic insulin sensitivity and suppression of EGP, consistent with previous studies (30). In fact, suppression of EGP may be more important for reducing postprandial glucose excursions than splanchnic glucose clearance (30,31). The reduction in glucose Ra iAUC may have also been due to reduced glucose absorption, which was previously shown after treadmill exercise at 70% V˙O2max in healthy subjects (32). Overall, our data suggest that RE may reduce the glycemic response to a mixed meal in men with prediabetes, which is consistent with the findings of Lopez et al. (9) and Andersen et al. (10) in healthy individuals. Because individuals with T2D demonstrate delayed suppression of endogenous postprandial glucose production and diminished glucose clearance, this could be an important means of preventing disease progression (33).

The increase in plasma glucose after intense, acute exercise is typically matched by a similar spike in plasma insulin concentration to restore normoglycemia and promote glucose uptake, which we observed in this study (34). It was previously reported that glucose concentrations return to baseline within 1–2 h of RE cessation, which we observed in our study by the end of the 3-h recovery period (34). However, the total glucose Ra remained significantly elevated even 3 h post-RE. The return of plasma glucose to baseline, therefore, appears to be due to a concomitant, significant increase in plasma glucose Rd measured at the end of the basal period. The elevation in basal glucose Ra may be a consequence of the lingering influence of exercise-stimulated gluconeogenic hormones (as discussed above).

Postprandial insulin concentrations were reduced after acute RE (main effect of treatment and reduced AUC and iAUC). This reduction seemed to occur more so during the later-phase insulin response (insulin secreted over the final ~5 h of the postprandial period) to the mixed meal, as the insulin concentrations were virtually equivalent between conditions over the first 45 min of the postprandial period, and there was no effect of RE on the insulinogenic index. These results are similar to those reported by Rynders et al. (35), who found a 26% reduction in the late-phase insulin AUC during a 3-h OGTT after bicycle ergometer exercise in prediabetic men and women. In the subsequent 5.5 h in our study, the reduction in plasma insulin in the RE condition is most likely due to the increase in insulin sensitivity, which would require less insulin per unit of glucose, thereby lowering the glucose-normalized insulin response. These results are also consistent with the findings of Phillips et al. (36), who found that a single bout RE at 65% and 85% 1RM significantly reduced plasma insulin concentration without changing the plasma glucose concentration response to a 100-g carbohydrate meal in young, recreationally active men, indicative of improved insulin sensitivity.

Multiple studies have reported that during the recovery from a single bout of RE, the contribution of lipid oxidation to total energy expenditure increases, whereas the contribution from carbohydrate oxidation decreases in healthy subjects, which is attributed to 1) the shunting of plasma glucose toward glycogen resynthesis, 2) postexercise increases in circulating free fatty acids (FFA; which can persist for 3–6 h), and 3) declines in plasma insulin (which inhibits lipid oxidation) (37). However, although we found that acute RE significantly increased whole-body lipid oxidation (15), it did not significantly reduce carbohydrate oxidation compared with rest (e.g., no difference in CHO oxidation at 140 min in Fig. 4A). Our results coincide closely with those of Gaitán et al. (38), who reported increased lipid oxidation but no change in carbohydrate oxidation in men and women with prediabetes after continuous and interval aerobic exercise training (2 wk). The difference in the relative contribution of lipid versus carbohydrate to oxidative metabolism in subjects who are healthy versus those who have prediabetes is unclear but could be related to differences in fatty acid metabolism. As we reported previously, we did not observe increases in circulating FFA after RE, which could be due to the shunting of circulation away from adipose tissue during the higher-intensity RE session, thereby limiting the ability of liberated FFA to enter the circulation to support lipid oxidation and requiring some additional glucose oxidation to support ongoing metabolism (39). We also identified a transient spike in postexercise plasma glucose and insulin concentrations immediately postexercise. Insulin is known to inhibit lipid oxidation and could therefore blunt the maximal increase in lipid oxidation (though the increase is still significant vs rest) after RE, thereby requiring increased supplemental glucose oxidation (40). These findings are most likely due to the absence of sampling carbohydrate oxidation for the first 170 min of the recovery period, as even in healthy subjects changes in oxidation approach baseline levels by 120 min post-RE (41).

By contrast, after meal ingestion, the total carbohydrate oxidation rate increased in both the rest and exercise conditions (sudden rise observed from min 140 to min 240), which is consistent with previous studies showing that carbohydrate ingestion promotes a rapid shift toward carbohydrate oxidation due to the preferential oxidation of glucose over FFA (42). However, we observed that peak carbohydrate oxidation was delayed in the RE condition, which may be caused by the prolonged increase postprandial lipid oxidation (15). This is supported by our observation that the percentage of maximal mitochondrial respiration from pyruvate oxidation was lower after RE, which, when combined with our previous findings of an increased lipid to glucose oxidation ratio (15), suggests that exercise increased the preference for oxidizing fatty acids even 90 min after meal consumption. The trend toward higher pyruvate-supported respiration after RE in this study may have been due to an elevation in total maximal mitochondrial oxidative capacity, as we reported previously (15). Although the mechanism of this fatty acid respiratory preference is unclear, elevations in beta-oxidation could lead to the accumulation of acetyl-CoA and citrate, which are known to activate pyruvate dehydrogenase kinase to inhibit the pyruvate dehydrogenase complex, mitigating glycolysis and glucose oxidation (43). Further investigation is needed to elucidate the mechanisms of postprandial fuel selection preferences in skeletal muscle after RE.

Finally, although we observed improvements in postprandial insulin sensitivity, insulin concentrations, postprandial glucose Ra, and glucose concentration normalized to premeal levels, it is appears that these benefits are waning by the end of the ~9-h study period. Previous studies have shown that RE can lower plasma glucose concentrations for 24 h post-RE (44), whereas others have reported no improvement in 24-h glucose (45). Because we did not analyze time points beyond the 6-h postprandial period, it is unclear if these benefits carry over the subsequent meals, which could contribute to improved 24-h glycemic control. In healthy subjects, a single bout of RE reduces plasma glucose for 14–24 h (10,46), suggesting that the duration of improvement is consistent with healthy populations. Overall, the effects of RE appear to dissipate by ≤24 h, indicating the that repeated bouts (i.e., training) are required for prolonged improvement.

In conclusion, we found that a single bout of RE moderately reduced the glycemic response to a mixed meal, significantly improved insulin sensitivity, and reduced the glucose-normalized insulin response in obese, middle-age men with prediabetes. However, there were limited effects on postprandial glucose clearance, insulinogenic index, whole-body carbohydrate oxidation, or skeletal muscle pyruvate-supported respiration. Further investigation is needed to elucidate how RE affects exogenous (meal) versus endogenous postprandial glucose metabolism, and if additional bouts of exercise (i.e., training) produce superior outcomes for this population.

LIMITATIONS

There are several limitations to this study. First, because no oral glucose tracer was used, we could only assess total glucose Ra without resolution of exogenous oral glucose appearance from EGP, which would be important to determine changes in postprandial hepatic insulin sensitivity. This study only enrolled adult men; thus, the results cannot be generalized to obese women or children with prediabetes. In addition, the study sample size was based on anticipated differences in glucose Rd after RE and not powered to detect differences in other variables. Despite this, we found differences in peripheral insulin sensitivity and glucose tolerance after RE. In addition, participants were required to fast for >15 h before the test meal to avoid the confounding effects of preexercise meals on postexercise postprandial glucose metabolism. Fasting for this duration is associated with a switch toward increased lipid oxidation as glycogen is depleted in the liver, skeletal muscle insulin resistance (secondary to the presence of increased FFA levels, increased fat oxidation and low glucose and insulin levels), and diminished carbohydrate oxidation (47,48). However, it was previously reported that changes in postexercise postprandial glucose concentrations were equivalent when participants were provided with a meal versus no meal before the exercise session. Therefore, although fasting could have affected some measures in this study, the magnitude of the effect is likely not large enough to significantly affect our findings.

This work was supported by the National Institutes of Health grant nos. T32 HD007434-25 (Lang), UL1TR000448 (Powderly), and P30 DK056341 (Washington University Nutrition Obesity Research Center) and the Foundation for Physical Therapy Promotion of Doctoral Studies II Scholarship (A. Bittel).

The authors thank Miranda Giuffrida and Kathryn Gratza for collecting muscle and adipose tissue biopsies, Lala Ruvinov for preparing all stable isotope tracers for infusion for this study, Sue Waller for preparing the standardized meals and the liquid test meals, Terri Pietka for assisting with mitochondrial respiration analysis, and Jennifer Shew and Freida Custodio for their help with sample preparation for GC/MS analysis (all Center for Human Nutrition, Washington University School of Medicine, St. Louis, MO). They also thank the nursing staff at the Washington University Institute for Clinical and Translational Sciences Clinical Research Unit (Washington University School of Medicine, St. Louis, MO) for their assistance with all study procedures.

The authors declare that there is no duality of interest associated with this manuscript.

The data sets generated/analyzed during this study are available from the corresponding author on reasonable request.

The authors have no conflicts of interest or significant financial support for this work that could have influenced its outcome.

The results of the study are presented clearly, honestly, and without fabrication, falsification, or inappropriate data manipulation. Results of the present study do not constitute endorsement by the American College of Sports Medicine.

A. J. B., D. C. B., B. M., B. W. P., and WTC designed the study. A. J. B., D. C. B., D. N. R., and W. T. C. conducted the studies. A. J. B., D. C. B., B. W. P., A. L. O., N. A. A., and W. T. C were involved in sample processing and analysis. A. J. B. performed the statistical analyses. A. J. B. drafted the manuscript, and D. C. B., B. M., B. W. P., A. L. O., N. A. A., D. N. R., and W. T. C. critically reviewed the manuscript for important intellectual content and approved the final version to be published. A. J. B. is the guarantor of this work and, as such, had full access to all the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis.

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Keywords:

PREDIABETES; POSTPRANDIAL; GLUCOSE; RESISTANCE EXERCISE; INSULIN

Copyright © 2020 by the American College of Sports Medicine