Journal Logo

SPECIAL COMMUNICATIONS: Corrigendum

Exhaustive Exercise Induces Gastrointestinal Syndrome through Reduced ILC3 and IL-22 in Mouse Model—Corrigendum

Medicine & Science in Sports & Exercise: March 2021 - Volume 53 - Issue 3 - p 671-672
doi: 10.1249/MSS.0000000000002600
  • Free

The authors of “Exhaustive Exercise Induces Gastrointestinal Syndrome through Reduced ILC3 and IL-22 in Mouse Model” (1) report an error Figure 3. The amended figure is presented hereinafter.

FU1
FIGURE 3:
Exhaustive exercise results in a reduced IL-22 level and ILC3 in the LPL. A, The relative mRNA expression levels of IL-22 in intestine tissues were assessed by qRT-PCR assay. B, Intestinal LPL was stimulated by phorbol-12-myristate 13-acetate, IL23 (50 ng·mL−1), and ionomycin with Golgi Stop for 4 h. IL-22 expression in CD45+ cells was analyzed by flow cytometry. Experiments were independently repeated at least three times. C, Frequencies of IL-22–expressing cells in the CD45+-gated cell populations of the intestines were shown. D, IL-22 expression in CD3+CD4+ cells was analyzed by flow cytometry (cells were gated by CD45). Experiments were independently repeated at least three times. E–F, Frequencies of IL-22–expressing cells in the CD3+CD4+-gated cell populations of the intestines were shown. G–H, The relative mRNA expression levels of IL1β and IL23 in intestine tissues were assessed by qRT-PCR. I, ILC3 (CD45+CD3CD127+RORγt+), NCR+ILC3 (CD45+CD3CD127+RORγt+ Nkp46+CD4), and LTi (CD45+CD3CD127+RORγt+Nkp46CD4+) in intestine LPL were analyzed by flow cytometry. Experiments were independently repeated at least three times. J–K, Percentages of all cell populations of the intestine LPL from the NE and EE group mice were shown. *P < 0.05, **P < 0.01, ***P < 0.001. Two groups were compared by independent-samples Student’s t-test for all statistical analyses.

REFERENCE

1. Hou P, Zhou X, Yu L, et al. Exhaustive exercise induces gastrointestinal syndrome through reduced ILC3 and IL-22 in mouse model. Med Sci Sports Exerc. 2020;52(8):1710–8.
Copyright © 2021 by the American College of Sports Medicine