IASTM intervention for the calf included gentle and firm strokes that applied compression and shear stress to produce a pulling force in the engaged tissues around the edges of the instrument (5). IASTM was performed with the therapist moving the knee and ankle joints to arbitrary joint angles according to the target tissue condition so that the position of the soft tissues ranged from a slightly extended position to a slightly shortened position (knee joint: 90° flexion to fully extended [0°]; ankle joint: resting position of plantarflexion [approximately 30°] to anatomical position [0°] and anatomical position to valgus (pronation) of approximately 5° in conjunction with the knee joint angle). Occasionally, IASTM was performed while moving the ankle and knee joints so that the soft tissues were slowly stretched from a slightly shortened position to a slightly extended position. The intervention was aimed at reducing fascia gliding limits as follows: first, very superficial light strokes were performed with the edge of the instrument on the skin to the subcutaneous structures, which was imbricated consecutive strokes; then similar but slightly deeper strokes were performed on the gastrocnemius; subsequent targets involved the SOL fascia and deeper to the posterior tibialis and flexor pollicis longus; finally, strokes were performed at the boundary of the posterior and lateral compartments and medial margin of tibia with the contiguous soft tissue.
The strokes of IASTM were concentrated between each calf muscles and the adjacent (contiguous) tissues, the muscle belly of each muscle, the boundaries between the muscles, and the muscle–tendon junction. The imbricated consecutive strokes were longitudinal straight or drawing an arc shape crossing the muscle or tendon fibers. Stroke pressure and speed were adjusted according to the subject’s tolerance so as not to cause pain. To evaluate subjective pain, we used scores of 1–5 on the visual analog scale (1, no pain at all; 5, intolerable discomfort; 0.5 increments), and IASTM was performed with scores <4 (discomfort [defined as feeling pain]). In clinical practice, IASTM is sometimes performed while maintaining the joint angle at which the muscles and tendon are stretched. In this study, we proceeded from the surface to the deeper structures with IASTM; however, we were careful to avoid the effects of stretching (e.g., the ankle was passively moved from the resting position to the neutral position without resulting in dorsiflexion). As the control condition, the subjects rested on a bed in the prone or supine position for 5 min between before and after measurements without IASTM while maintaining the plantarflexors in the relaxed state (knee joint angle, slightly flexed; ankle joint angle, approximately 30°) to avoid their stretching.
Dorsiflexion range of motion and ankle joint stiffness
The ankle dorsiflexion range of motion and the ankle joint stiffness were measured with an isokinetic dynamometer (Biodex System 3; Biodex Medical Systems, Shirley, NY). The subject was seated with the knee fully extended (hip joint angle, 60° flexion), and the thigh and foot were fixed to the dynamometer with a belt. The ankle joint was dorsiflexed from 0° (anatomical position) to the angle at which the subject felt discomfort (peak dorsiflexion angle) at a velocity of 2°·s−1; the range from 0° to the peak dorsiflexion angle was defined as the dorsiflexion range of motion. Visual analog scale (described above) was used for subjective pain assessment (4, discomfort). Torque in the direction of plantarflexion during peak dorsiflexion was defined as peak passive torque. This measurement was repeated twice; if there was a difference of ≥10% in the dorsiflexion range of motion, the measurement was performed a third time. The two values obtained for the dorsiflexion range of motion were averaged, as were the two values obtained for peak passive torque; these means were used as representative values. The subjects were instructed to relax during the measurements without resisting passive dorsiflexion. The output of torque and ankle joint angle signals by the isokinetic dynamometer were converted to digital signals at 2 kHz via an analog-to-digital converter (PowerLab/16SP; ADInstruments, Sydney, Australia) and recorded on a personal computer (FMV Lifebook, Fujitsu, Japan) using an analysis software (LabChart 7, ADInstruments).
The activities of the lower leg muscles while measuring the dorsiflexion range of motion were recorded with surface electromyography. Electromyograms were obtained using a Delsys EMG data acquisition system (Bagnoli-8; Delsys, Inc., Boston, MA). Active surface electrodes (interelectrode distance of 10 mm, DE-2.1; Delsys, Inc.) were lightly polished and cleaned with ethyl alcohol and were attached to the muscle bellies of the MG, LG, SOL, and tibialis anterior. As with the dynamometer data, these electromyogram signals were converted to a digital format at a sampling frequency of 2 kHz using an analog-to-digital converter and were then recorded on a personal computer using the same analysis software (data smoothing on the software: band-pass filter, passband at 25–450 Hz). Root mean square (RMS) values of muscle activity during passive dorsiflexion were determined from four joint angle ranges (described above) from the electromyogram signals. These RMS values were normalized according to the RMS value during maximum voluntary contraction (MVC) of ankle plantarflexor and dorsiflexor muscles. To measure the RMS value during MVC of ankle plantarflexors and dorsiflexors, the maximal strengths of plantarflexion and dorsiflexion were evaluated using a dynamometer (described above), with the knee fully extended in the sitting position and the ankle at 0°. Before evaluating the maximal strength of plantarflexion, the subjects were instructed to practice and produce force less than that required during maximal effort (80% of maximum) twice. After at least 1-min rest, the subjects performed two exertions of maximal strength of plantarflexion with 1-min rest between trials. Peak torque was analyzed, and the RMS value of the trial with the higher peak torque was used for normalization. Ankle dorsiflexion MVC was also similarly performed. On the basis of these tests, rest of more than 15 min was provided between MVC and preintervention measurements to minimize the effect of MVC measurement procedures on the ROM and muscle stiffness measurements.
Ankle joint stiffness was calculated from the torque and joint angle obtained while measuring the dorsiflexion range of motion. On the basis of a previous study (13), with the peak dorsiflexion angle in each dorsiflexion range of motion measurement defined as 100%, we determined joint angles at 0%, 33%, 66%, and 100% as well as the passive torque at each of these angles. With these joint angles defined as angles 1–4, the respective mean joint angles were as follows: 1) 0° ± 0°, 2) 11° ± 3°, 3) 22° ± 7°, and 4) 32° ± 10°. The slope of the dorsiflexion angle-passive torque relationship at angles 1–4 was defined as ankle joint stiffness. This analysis was conducted for the two trials that adopted the dorsiflexion range of motion; ankle joint stiffness was defined as the mean of values obtained in these two trials.
The stiffness of MG and SOL were measured using shear wave elastography (Aixplorer MSK mode; SuperSonic Imagine, Aix-en-Provence, France) (Fig. 3). For the measurement, the ankle joints were at 0° and 25° dorsiflexion, based on a previous study (14), while the subjects were in the same posture as they were during the dorsiflexion range of motion measurement. The shear modulus of MG was measured at the center of the muscle belly (at 30% of the proximal length of the leg), and the shear modulus of SOL was measured near the distal end of the muscle belly of MG. The preintervention measurement sites were marked with a marker, and a linear ultrasound probe (frequency, 4–15 MHz; scan width, 50 mm; SL15-4, SuperSonic Imagine) was placed at that site during the postintervention measurement. The shear moduli of MG and SOL were measured five times each; the highest and the lowest values were excluded, and the mean of the remaining three values was used as the representative value. To determine the shear modulus, Young’s modulus obtained from the shear wave elastography device was divided by 3 (E = 3ρc2; Young’s modulus, E; tissue density, ρ; shear wave velocity, c). The region of interest of shear modulus was defined as a circle with a diameter of 5 mm (15).
All data are expressed as mean ± SD. Two-way repeated-measures ANOVA (IBM SPSS statistics 24; SPSS Japan, Japan) for all pre- and postintervention measurements was performed by intervention condition (IASTM and control)–time (pre- and postintervention) interaction. If an interaction or a main effect of time was observed, the paired t-test was performed for each condition. The paired t-test was also performed to confirm that there were no differences in any preintervention measured values between the conditions. As the effect size, Cohen’s d (for post hoc comparisons) was calculated by the following formula: d = Mdiff/SDpooled √2[1 − r], where Mdiff is the difference between mean pre- and postmeasurement value and r is the correlation between means (16). As a result of a priori statistical power analyses, it was estimated that 14 subjects were required for this study design in each of the two conditions (repeated-measures ANOVA within factors; effect size, 0.4; power, 0.8; alpha level, 0.05 [(17)]); using G*power 3). The effect size (d) of the paired t-test was calculated by dividing the difference between the average values of the pre- and postmeasurement values by the SD of the premeasurement value. The effect size was defined as follows: |0.20|–|0.50| small effect, |0.50|–|0.80| medium effect, and |0.80|< large effect (17). The level of statistical significance was set at P < 0.05.
An interaction between condition and time was observed in the dorsiflexion range of motion (P = 0.018, ηp2 = 0.36), which significantly improved after IASTM (31.6° ± 9.8° to 35.0° ± 9.0°, P < 0.01, d = 1.15) but was not observed in the control condition (31.7° ± 10.8° to 32.6° ± 9.2°, P = 0.15) (Fig. 4). At peak passive torque, there was no interaction between condition and time or main effect of time; no changes in peak passive torque were observed in any condition (interaction: P = 0.67, ηp2 = 0.015; main effect of time: P = 0.23, ηp2 = 0.11) (Table 1). A main effect of time was observed in ankle joint stiffness (P = 0.035, ηp2 = 0.30), which decreased significantly after IASTM (1.29 ± 0.44 N·m·deg−1 to 1.19 ± 0.38 N·m·deg−1, P = 0.047, d = −0.62), but did not change in the control condition (1.26 ± 0.38 N·m·deg−1 to 1.24 ± 0.39 N·m·deg−1, P = 0.20) (Fig. 4).
In muscle stiffness of MG at joint angles of 0° and dorsiflexion of 25°, no interaction was observed between condition and time, and no main effect of time was observed; there were no changes in either condition (0°; interaction: P = 0.15, ηp2 = 0.16; main effect of time: P = 0.89, ηp2 < 0.01) (dorsiflexion 25°; interaction: P = 0.44, ηp2 = 0.047; main effect of time: P = 0.88, ηp2 < 0.01) (Table 1). Similarly, in muscle stiffness of SOL at joint angles of 0° and dorsiflexion of 25°, there were no changes in any condition (0°; interaction: P = 0.76, ηp2 < 0.01; main effect of time: P = 0.60, ηp2 = 0.022) (dorsiflexion 25°; interaction: P = 0.67, ηp2 = 0.015; main effect of time: P = 0.54, ηp2 = 0.030) (Table 1).
This study demonstrated that IASTM increases the dorsiflexion range of motion and decreases ankle joint stiffness. In addition, this study clarified that peak passive torque during the measurement of the dorsiflexion range of motion and muscle stiffness of MG and SOL was not affected by IASTM.
Some previous studies have reported that IASTM improves joint range of motion (4,5). The results of this study support the results of the previous studies. However, another previous study reported that IASTM (Graston Technique®), which was performed on the posterior part of the lower leg similar to that in the present study, resulted in no improvement in the dorsiflexion range of motion (6). Therefore, we believe that the dorsiflexion range of motion was improved by IASTM in this study as the participants were healthy without a clinical level of joint range of motion restriction. Further studies are required to clarify whether the magnitude of the effect of IASTM on flexibility is related to the previous level of flexibility of the subject. The activations of the triceps surae muscles during ankle dorsiflexion were quite low in pre- and postintervention measurements (Table 2). This result demonstrates that there was almost no input from the central nervous system to the triceps surae. In addition, the dorsiflexion range of motion improved by 10.7% ± 10.8% after IASTM, which is approximately 16% lesser than that reported in some previous studies that measured it after static stretching (18,19). This may be because IASTM (−6.2% ± 10.1%) results in a lesser reduction in ankle joint stiffness than static stretching (approximately −12%) (19). Furthermore, unlike static stretching, IASTM does not change the stiffness of the muscle belly and stretch tolerance. However, it has been reported that IASTM (Graston Technique®) effectively improves the flexibility of the hip joint in patients with chronic low back pain in comparison with static stretching (20). Therefore, IASTM appears to be less effective in improving flexibility than static stretching, and it depends on the target sites and subjects.
It is believed that reduction in joint stiffness is secondary to a decrease in the stiffness of the muscles and tendons constituting the MTU across the joint (7,8). The results of the present study revealed that IASTM reduces joint stiffness, which contributes to the improvement of joint range of motion. However, muscle stiffness of MG and SOL was unchanged after IASTM. In this study, IASTM was not performed over the feet and ligaments of the ankle joint. Therefore, we believe that the stiffness of soft tissue other than that of the muscle belly of the triceps surae (such as the Achilles tendon) decreased after IASTM. One of the physiological hypotheses regarding IASTM is reduced sliding resistance that occurs between muscle and deep fascia when the muscle contracts or stretches (21), which could be one of the causes of decreased joint stiffness in the present study.
Previous studies have suggested that manual and roller massages increase stretch tolerance or inhibit pain perception (11,12). However, in the present study, peak passive torque during ankle dorsiflexion remained unchanged after IASTM, indicating that IASTM did not change the perception of pain caused by stretching the MTU (stretch tolerance). This suggests that the stroking action of IASTM resulted in only shallow penetration of the muscle and, therefore, did not change the sensitivity of the mechanoreceptors. Stretch tolerance is known to increase after static stretching (stretching stress load to the MTU) (8,9). Therefore, the results of this study suggest that IASTM does not induce stretching stress over the MTU, which affects stretch tolerance. In addition, it is expected that a combination of IASTM and stretching could have greater effect on flexibility than IASTM or stretching alone. Changes in the stretch-reflex sensitivity (H-reflex) are also considered a part of the mechanism for improvement in the joint range of motion (7,8). Previous studies have reported temporary decreases in the H-reflexes of target muscles during manual and roller massages (12,22). These studies have suggested that massaging increases Ib afferent inhibition and presynaptic inhibition by modulating the sensitivity of the Golgi tendon organs and pressure sensitive receptors of the skin and muscle (12,22). Therefore, further studies are required to clarify the effects of IASTM on H-reflex.
It has been found that static stretching reduces neuromuscular activity and maximal voluntary force production (8,10). These negative effects after stretching are presumed to be secondary to changes in neural properties such as reduced central drive, lowered stretch-reflex sensitivity, and increased stretch tolerance (8,23,24). On the other hand, IASTM was found to improve joint range of motion without changing stretch tolerance in this study. In the present study, we prioritized the verification of the effects of IASTM on ankle joint range of motion and neuromechanical properties of the triceps surae and did not measure muscle strength or power. Further studies are required to clarify whether IASTM affects muscular performance such as strength and power.
In the present study, the pressure or force of the instrument was not measured; therefore, future studies should evaluate them quantitatively and objectively elucidate the effective stroke intensity. The intensity of IASTM was determined by the therapist and the respective subject in this study. It should be noted that these findings were obtained as a result of the use of IASTM alone in healthy subjects by an experienced therapist. In routine practice, IASTM is sometimes combined with compressive myofascial release such as massage and stretching. Therefore, IASTM in routine practice may have a greater effect on joint mobility than that observed in this study.
This study revealed that IASTM over the posterior part of the lower leg can improve the ankle joint dorsiflexion range of motion and stiffness, without affecting stretch tolerance, and muscle stiffness of MG and SOL.
The authors sincerely thank Naoya Ebi (former undergraduate at the School of Sport Science, Waseda University) and the participating volunteers (former students at the Waseda University) for their cooperation. This study was supported by JSPS KAKENHI (grant number 16H01870); Grant-in-Aid for Scientific Research [A]. This study was part of research activities of the Human Performance Laboratory, Organization for University Research Initiatives, Waseda University. The results of this study are presented clearly, honestly, and without fabrication, falsification, or inappropriate data manipulation, and the results of the present study do not constitute endorsement by the American College of Sports Medicine.
The authors declare no conflict of interest. None of the authors has a professional relationship with any company or manufacturer who will benefit from the results of the present study.
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Keywords:© 2019 American College of Sports Medicine
SHEAR WAVE ELASTOGRAPHY; PLANTARFLEXOR MUSCLES; JOINT AND MUSCLE STIFFNESS; ELECTROMYOGRAPHY; STRETCH TOLERANCE; RANGE OF MOTION