G-43b Free Communication/Poster - Late-Breaking Abstracts Saturday, June 1, 2019, 7: 30 AM - 11: 00 AM Room: CC-Hall WA2
Vaccination is one of the most successful public health interventions for preventing infectious diseases, although the immunosuppressive effects of chronic stress can reduce a vaccine's efficacy. Exercise improves vaccine responses, but the role in attenuating stress-induced effects is unknown.
PURPOSE: We investigated the effects of forced/acute (eccentric exercise; ECC) and voluntary/long-term (wheel running; VWR) exercise on antibody and cell-mediated immune responses to vaccination in chronically stressed mice.
METHODS: Mice were randomized into Control (CON), S (Stress)-ECC, S-VWR, and S-SED (Sedentary) groups. Chronic restraint stress occurred 6-h/day, 5-days/week for three weeks. S-VWR mice were allowed access to a wheel for the entire experiment. One week post-stress, S-ECC mice ran on a treadmill for 17m/min, -20% grade, for 45 minutes and were then injected with 100μg of ovalbumin (OVA) and 200μg of alum adjuvant (intramuscularly), along with all other groups. Anti-OVA IgM and IgG was measured via ELISA. Three weeks post-stress, mice were injected with OVA into the ear to determine delayed-type hypersensitivity (DTH) response as a measure of cell-mediated immunity.
RESULTS: As expected, chronic restraint stress significantly reduced body weight and caused adrenal hypertrophy. Over the course of the experiment, S-ECC, and S-VWR groups had significantly elevated anti-OVA IgG compared to S-SED which had significantly lowered levels compared to CON (p < 0.05). No differences were observed with anti-OVA IgM nor DTH responses.
CONCLUSION: Acute ECC and VWR alleviated chronic stress-induced reductions in anti-OVA IgG vaccination responses while neither type of exercise had an impact on anti-OVA IgM or cell-mediated immune responses. Future experiments need to address the mechanism of the exercise beneficial effects on IgG.