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Effect of MCT-1 Polymorphism on Lactic Acid Clearance in Resistance Trained Females

2641 Board #305 May 31 11:00 AM - 12:30 PM

Branon, Lauren M.

Medicine & Science in Sports & Exercise: June 2019 - Volume 51 - Issue 6 - p 737
doi: 10.1249/01.mss.0000562701.38862.47
E-44 Free Communication/Poster - Immunology III Friday, May 31, 2019, 7: 30 AM - 12: 30 PM Room: CC-Hall WA2

University of Southern Indiana, Evansville, IN.


(No relationships reported)

The monocarboxylate transporter one (MCT-1) protein is a carrier protein that plays a vital role in cellular respiration. MCT-1 is known to aid in the transportation of carboxylates, including lactic acid, out of the blood stream and back into muscle tissue.

There is a common single-nucleotide polymorphism (SNP) in the MCT-1 gene (T1470A) that is believed to play a role in the clearance rate of circulating lactic acid following intense exercise.

PURPOSE: The purpose of this study was to investigate the role of the MCT-1 SNP on lactic acid production and clearance rates in resistance trained, females.

METHODS: Twenty-one female participants (age= 20.81+/- 2.06 years) who resistance train three or more times per week were recruited for this study. Lactic acid measurements were taken pre-test and 0, 10, 20, 30, 40 minutes post-test. In order to induce lactic acid production, a standard thirty second Wingate test was used. The MCT-1 gene of each participant was isolated from saliva and amplified using Polymerase Chain Reactions (PCR). Once isolated and amplified, each participants MCT-1 gene was sequenced using Next Generation Sequencing. A two-way mixed factorial ANOVA [genotype (AA,TA,TT) x time (pre, 0, 10, 20, 30, 40)] was used to examine interactions between genotype and time. Three follow-up repeated measures ANOVA's (individual groups x time) were utilized to examine how each group contributed to this interaction. Alpha values were set at .05 and Bonferonni corrections were used in all analyses.

RESULTS: Further, a significant interaction (genotype x time) was observed (p=0.001). Follow-up ANOVA indicated that each had a main effect for time (p=0.001 for each group). Additionally, the AA group cleared lactic acid at a significantly faster rate (p=0.01) than the TT group for the 10-20 minute time period.

CONCLUSION: These results indicate that the MCT-1 SNP likely plays a role in lactate clearance in resistance trained females.

© 2019 American College of Sports Medicine