E-44 Free Communication/Poster - Immunology III Friday, May 31, 2019, 7: 30 AM - 12: 30 PM Room: CC-Hall WA2
The monocarboxylate transporter one (MCT-1) protein is a carrier protein that plays a vital role in cellular respiration. MCT-1 is known to aid in the transportation of carboxylates, including lactic acid, out of the blood stream and back into muscle tissue.
There is a common single-nucleotide polymorphism (SNP) in the MCT-1 gene (T1470A) that is believed to play a role in the clearance rate of circulating lactic acid following intense exercise.
PURPOSE: The purpose of this study was to investigate the role of the MCT-1 SNP on lactic acid production and clearance rates in resistance trained, females.
METHODS: Twenty-one female participants (age= 20.81+/- 2.06 years) who resistance train three or more times per week were recruited for this study. Lactic acid measurements were taken pre-test and 0, 10, 20, 30, 40 minutes post-test. In order to induce lactic acid production, a standard thirty second Wingate test was used. The MCT-1 gene of each participant was isolated from saliva and amplified using Polymerase Chain Reactions (PCR). Once isolated and amplified, each participants MCT-1 gene was sequenced using Next Generation Sequencing. A two-way mixed factorial ANOVA [genotype (AA,TA,TT) x time (pre, 0, 10, 20, 30, 40)] was used to examine interactions between genotype and time. Three follow-up repeated measures ANOVA's (individual groups x time) were utilized to examine how each group contributed to this interaction. Alpha values were set at .05 and Bonferonni corrections were used in all analyses.
RESULTS: Further, a significant interaction (genotype x time) was observed (p=0.001). Follow-up ANOVA indicated that each had a main effect for time (p=0.001 for each group). Additionally, the AA group cleared lactic acid at a significantly faster rate (p=0.01) than the TT group for the 10-20 minute time period.
CONCLUSION: These results indicate that the MCT-1 SNP likely plays a role in lactate clearance in resistance trained females.