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Longitudinal Physical Activity, Body Composition, and Physical Fitness in Preschoolers

LEPPÄNEN, MARJA H.1; HENRIKSSON, PONTUS2,3,4; DELISLE NYSTRÖM, CHRISTINE4; HENRIKSSON, HANNA2,3; ORTEGA, FRANCISCO B.3; POMEROY, JEREMY5; RUIZ, JONATAN R.3; CADENAS-SANCHEZ, CRISTINA3; LÖF, MARIE2,4

Author Information
Medicine & Science in Sports & Exercise: October 2017 - Volume 49 - Issue 10 - p 2078-2085
doi: 10.1249/MSS.0000000000001313

Abstract

Childhood obesity is a growing public health problem all over the world (40), and it has been associated with many physical and psychological consequences, such as metabolic risk factors and decreased health-related quality of life (32). Overweight and obesity have been found to progress from early childhood to adolescence (26,33), and due to their influence on energy balance, increasing knowledge of associations between physical activity (PA) and sedentary behavior (SB) with adiposity already in young children is needed. Our recent cross-sectional study in 4.5-yr-olds (22) found a strong positive relationship between vigorous-intensity PA (VPA) and fat-free mass index (FFMI), whereas moderate-to-vigorous PA (MVPA) was negatively associated with fat mass percent (%FM). Similar findings have also been reported in other cross-sectional studies in preschool-age children (3,4,9,10,20,38), but the relationship between SB and adiposity is still inconsistent (4,9,10,14,20,22). However, only a few studies in preschoolers have examined longitudinal associations between PA and adiposity, reporting negative associations (19,25), and no association (3,24) as well as a positive association between SB and body mass index (BMI) (19). These studies have used subjective measurement of PA and SB (19) as well as BMI (19,24) or bioimpedance (3) in estimating body fatness, which may not be an accurate measurement in children (12,16). Thus, further longitudinal studies using objective and accurate measurements of PA, SB, and body composition in preschoolers are needed to investigate whether PA and/or SB is longitudinally associated with body composition. Such knowledge may help health care professionals target actions for obesity prevention already at young ages.

Physical fitness has been recognized as an important marker of health (29,30,34). Previous cross-sectional studies (3,22) have reported that PA was positively associated with physical fitness in preschoolers, whereas a higher amount of self-reported PA has also been associated with better motor fitness (1). However, to our knowledge, there are no previous studies examining longitudinal associations of SB with physical fitness, and only one study has investigated the longitudinal associations of PA with limited components of physical fitness in preschool-age children (3).

Hence, the aim of this study was to investigate the longitudinal associations of PA and SB with body composition and physical fitness in healthy Swedish children 4.5 yr of age and 12 months later as a follow-up from our previous cross-sectional study (22). As an additional aim, we also studied whether a change in PA and SB between 4.5 and 5.5 yr was associated with corresponding change in body composition and physical fitness.

SUBJECTS AND METHODS

Study design and participants

The present study used baseline and 12-month follow-up data from the MINISTOP trial collected between 2014 and 2016, and the details of the trial have been described previously by Delisle et al. (11). Briefly, MINISTOP was a two-arm, randomized controlled parallel design and population-based trial in 315 healthy Swedish 4-yr-old children. The 6-month intervention consisted of a mobile phone-based application (the MINISTOP app) to help parents promote healthy eating and PA in children. The outcomes of the trial have been previously published (13).

Participants were recruited from the population register at Statistics of Sweden. Invitation letters (n = 3368) were sent in the spring of 2014 to the guardians (parents and other caretakers) of all 4-yr-old children living in the county of Östergötland, asking them to participate in the study. Parents were included in to the study if they had a healthy 4-yr-old child, had the possibility to have their child measured at baseline (approximately at 4.5 yr), and for at least one parent to able to speak and read Swedish sufficiently well. In total, 315 completed the baseline measures and were enrolled in the intervention study. Children in the control group (n = 159); that is, those who did not receive any intervention, were included in this study. Those with insufficient accelerometer data at baseline (n = 8) or lack of anthropometric measurements at follow-up (n = 13) were excluded from the analyses. In total, 138 children were included in the analyses for this study. Informed consent, witnessed and formally recorded, was obtained from all parents. The trial was registered at clinicaltrials.gov (NCT02021786) and approved by the Research Ethics Committee, Stockholm, Sweden (2013/1607–31/5; 2013/2250–32).

Data collection

The children’s PA, SB, body composition, and physical fitness were measured at the hospital in the same way at baseline and again after 12 months. PA and SB were assessed using the wrist-worn ActiGraph wGT3x-BT triaxial accelerometer (www.actigraphcorp.com), and children were asked to wear the accelerometer for 24 h over seven consecutive days. The monitors were set to sample at 50 Hz. Because the PA levels in our 138 children were similar on both week and weekend days (data not shown), children with at least 3 d of valid activity data, regardless of the day of the week, were included in the analyses. A valid day was defined as ≥600 min of awake wearing time (10). Nonwearing time was determined according to van Hees et al. (39), and the time scored as worn was classified into sleep or awake time using the Sadeh algorithm (35,36). ActiLife software (version 6.11.2) was used to process the raw data to derive filtered sum of vector magnitudes (VM) in 10-s epochs. The time spent (min·d−1) in intensity-specific PA levels and in SB were calculated for each child. The cut points were defined for SB as VM ≤305, for light-intensity PA (LPA) as VM 306–817, for moderate-intensity PA (MPA) as VM 818–1968, for VPA as VM ≥1969, and for MVPA as VM ≥818 in accordance with Chandler et al. (7).

Height and waist circumference were measured using standardized procedures as previously described (5). Body composition was assessed using air-displacement plethysmography by means of the pediatric option for BodPod (www.cosmed.com) (15). The body composition measures included were BMI, fat mass index (FMI), FFMI, and %FM. BMI was calculated as body weight (kg)/height2 (m), FMI as fat mass (kg) × height (m)−2, and FFMI as fat-free mass (kg) × height (m)−2.

Physical fitness was measured using four tests within the PREFIT fitness test battery (28): a 20-m shuttle run test for cardiorespiratory fitness, a handgrip strength test for upper body muscular strength, a standing long jump test for lower body muscular strength, and a 4 × 10-m shuttle run test for motor fitness (speed/agility). The tests were applied twice, except the 20-m shuttle run test, which was conducted once. Regarding handgrip strength test, the best value of the two attempts for each hand was selected, and the average of both hands was used in the analyses. For the standing long jump and the 4 × 10-m shuttle run tests, the best values of the two attempts were used in the analysis. The PREFIT test battery is based on a systematic review about the reliability and validity of physical fitness tests in preschool children (28), and it has been recognized as a feasible tool in assessing physical fitness in preschool-age children (6). The details of the measurements of PA, SB, body composition, and physical fitness have been previously published (5,22). In addition, parents reported their age, body weight, height, and education by means of a questionnaire.

Statistical methods

For this analysis, we considered that a sample size of 133 provides 80% power (two-tailed and α = 0.05) to detect a standardized regression coefficient of 0.24. Descriptive information is given as arithmetic means (SD) or frequencies (percentages). Linear regression was used to assess the association between intensity-specific PA (LPA, MPA, VPA, and MVPA) and SB at baseline with body composition measurements and physical fitness tests at the 12-month follow-up. At first, we fitted unadjusted models, and subsequently, each model was adjusted for child’s sex (boy or girl) and age (continuous) at the measurement and ActiGraph awake wearing time (continuous) because of potential effect on body composition and physical fitness. The models with SB, LPA, or MPA as exposures were also adjusted for VPA, whereas models with VPA or MVPA were adjusted for SB. In addition, we fitted isotemporal substitution models (23) to estimate the effect of substituting one PA type with another PA type at baseline on body composition or physical fitness at the 12-month follow-up. In supplemental analyses, we examined the associations of change in intensity-specific PA and SB with change in body composition and physical fitness between baseline and the 12-month follow-up. The change values for each variable were calculated as the 12-month follow-up value subtracted by the corresponding baseline value. In sensitivity analyses, we additionally adjusted the regression models for maternal BMI (continuous), maternal educational attainment (university degree or no university degree), paternal BMI (continuous), and paternal educational attainment (university degree or no university degree). Because the results were similar (data not shown), these variables were not included in the final adjusted models.

Sex comparisons between average values were made by using independent t-test for continuous variables and chi-square test for categorized variables. We also investigated if the associations differed by sex by adding an interaction term (type of PA–child’s sex) to the adjusted regression models. For these analyses, we considered P < 0.01 as the level of significance for the interaction terms to control for multiple comparisons. We did not find any evidence for any sex interactions, and consequently, we present the results for boys and girls together.

Control of regression models showed that required assumptions (independence, linearity, homoscedasticity, and normality) were not violated. All statistical tests were conducted using the two-sided 5% level of significance and performed using SPSS Statistics 24 (IBM, Armonk, NY).

RESULTS

Background characteristics

The average age of the participating children’s mothers (n = 138) at baseline was 35 yr (SD 4.3), height 167 cm (SD 0.1), weight 67 kg (SD 11.9), and BMI 23.8 kg·m−2 (SD 4.1), and more than half of them (n = 95, 68.8%) had a university degree. The fathers’ (n = 136) average age was 38 yr (SD 5.1), height 182 cm (SD 0.1), weight 84 kg (SD 13.0), and BMI 25.4 kg·m−2 (SD 3.5), and more than half of them (n = 76, 55.1%) had a university degree.

Table 1 describes the characteristics of the 138 children at baseline and at the 12-month follow-up and is subdivided by sex. Valid accelerometer data for the children were obtained for 3 (2.0%), 4 (2.9%), 5 (6.5%), 6 (13.0%), and 7 (75.4%) days at baseline.

T1-15
TABLE 1:
Characteristics of participating children at baseline and at 12-month follow-up.

Associations of PA and SB at baseline with body composition at 12-month follow-up

Table 2 presents the associations of intensity-specific PA and SB at baseline with body composition at the 12-month follow-up. Greater VPA was associated with higher BMI (P = 0.005), which was due to higher FFMI (P < 0.001) and not FMI (P = 0.79). Furthermore, greater MVPA was associated with higher FFMI (P = 0.044). No significant relationships between SB, LPA (data not shown), or MPA with FMI or %FM were discovered.

T2-15
TABLE 2:
Associations of accelerometer-derived PA intensitiesa at baseline at 4.5 yr with body composition and physical fitness at 12-month follow-up per 5-min increase in PA intensities.

Isotemporal substitution analyses (Fig. 1) showed that substituting 5 min·d−1 of SB, LPA, or MPA with 5 min·d−1 of VPA at the age of 4.5 yr was related to higher FFMI at 5.5 yr (P < 0.001 to P = 0.001) as well as to higher BMI (P = 0.003 to P = 0.006). By contrast, we found no statistically significant associations for FMI or %FM when substituting any of the PA (data not shown).

F1-15
FIGURE 1:
Isotemporal substitution analysis showing the associations of replacing 5 min·d−1 of one PA type with 5 min·d−1 of another PA type at baseline with body composition and physical fitness at the 12-month follow-up. The results are presented as beta-coefficients with 95% confidence intervals. N = 132–137.

Associations of PA and SB at baseline with physical fitness at 12-month follow-up

Cardiorespiratory fitness: Greater VPA and/or MVPA were associated with more laps in the 20-m shuttle run test (P = 0.016 and P = 0.014) (Table 2). However, as shown in Figure 1, when substituting any of the PA, there were no statistically significant associations observed. Upper muscular strength: Before adjusting for confounders, greater VPA was associated with better handgrip strength score (P = 0.002). However, after adjustments, the association disappeared (P = 0.083). Yet substituting 5 min·d−1 of SB, LPA, or MPA with 5 min·d−1 of VPA at the age of 4.5 yr was related to better handgrip strength at 5.5 yr (P = 0.026 to P = 0.046) (Fig. 1). Lower muscular strength: Greater VPA or MVPA was associated with longer jumps in the standing long jump test, respectively (P = 0.001 and P = 0.023) (Table 2). Substituting 5 min·d−1 of SB, LPA, or MPA with 5 min·d−1 of VPA at the age of 4.5 yr was associated with longer jumps at 5.5 yr, respectively (P = 0.002 to P = 0.014) (Fig. 1). Motor fitness: Greater VPA and MVPA were associated with faster time in the 4 × 10-m shuttle run test (P = 0.031 and P = 0.026, respectively) (Table 2). As presented in Figure 1, when substituting any of the PA, there were no statistically significant relationships observed.

Associations of change in PA and SB between baseline and 12-month follow-up with change in body composition and physical fitness

In addition, we examined associations of change in PA and SB between baseline and 12-month follow-up with the corresponding change in body composition and physical fitness as shown in Supplemental Table 1 (see Table, Supplemental Digital Content 1, Associations of change in accelerometer-derived PA intensities (per 5-min change) with change in body composition and physical fitness between baseline at 4.5 yr of age and the 12-month follow-up, https://links.lww.com/MSS/A952). When adjusting for confounders, increases in VPA over the follow-up period were associated with decrease in %FM and FMI (P = 0.004 and P = 0.048) as well as improved upper and lower muscular strength (P = 0.030 and P = 0.033, respectively). Furthermore, an increase in MVPA was associated with an increase in FFMI (P = 0.037) and a decrease in %FM (P = 0.031).

DISCUSSION

Our main finding is that higher VPA and MVPA at the age of 4.5 yr were associated with higher FFMI and better physical fitness at the 12-month follow-up. Furthermore, substituting 5 min·d−1 of SB, LPA, or MPA with VPA at 4.5 yr was associated with higher FFMI and greater upper and lower muscular strength at follow-up. The results support our hypothesis that high-intensity PA is longitudinally associated with a healthier body composition and better physical fitness.

We found that greater VPA or MVPA at the age of 4.5 yr was associated with higher FFMI at 5.5 yr. The finding is in line with our cross-sectional study (22) but additionally fills the current knowledge gap about the longitudinal association between PA and FFMI in young children. Because the previous studies (3,19,24,25) have not measured associations of PA with FFMI, our findings are novel. One important finding is that greater VPA at the age of 4.5 yr was positively associated with BMI at 5.5 yr. Because of the detailed body composition measurement, we were able to divide body weight into fat-free mass and fat mass. Because VPA in this study was associated with higher FFMI, but not FMI, we can conclude that the association between VPA and BMI was actually reflecting an association between VPA and FFMI. Previous studies (3,19,24) have examined longitudinal associations between PA and change in BMI, finding negative associations (19) as well as no association (3,24). However, the different study designs as well as methods in assessing PA and body composition may have led to the somewhat contradictory results. Thus, future studies should preferably include detailed body composition measurements, and not just BMI, together with objective measurements of PA. We did not find any significant associations between intensity-specific PA at the age of 4.5 yr and %FM or FMI at 5.5 yr, which is in line with Bürgi et al. (3). It is possible that young children’s nature of being active, short bursts of high level PA and varying intervals of different intensities, may not always be enough in reducing %FM. Indeed, low levels of PA have been related to higher subcutaneous fat by means of skinfolds (25); however, the skinfold technique has been found to be a less accurate method to assess body composition in preschoolers (18).

No statistically significant associations between SB at the age of 4.5 yr and body composition measurements at 5.5 yr were found. Jago et al. (19) has previously reported that SB, based on a direct observation, was positively associated with change in BMI. Because of the limitations concerning observation method (2), further studies are needed to investigate the longitudinal association between objectively measured SB and body composition, and to clarify what amounts of SB increases the risk of unhealthier body composition.

Greater VPA and MVPA at the age of 4.5 yr were significantly related to cardiorespiratory fitness at 5.5 yr. To our knowledge, only Bürgi et al. (3) have previously studied the association between baseline PA and change in cardiorespiratory fitness over a 9-month follow-up reporting a positively significant result. Our results confirm their findings and additionally expand the literature by providing data on muscular strength. In addition, we observed that substituting 5 min·d−1 of LPA for VPA at the age of 4.5 yr was associated with a better score in the 20-m shuttle run test at 5.5 yr. However, the result did not quite reach the significance (P = 0.056), but the tendency supports our hypothesis that high-intensity PA instead of LPA may also improve cardiorespiratory fitness in preschoolers. In addition, we observed that VPA or MVPA at the age of 4.5 yr was significantly related to better scores in lower muscular strength and motor fitness at 5.5 yr. This result is expected because the association between VPA and lower muscular strength was also very strong in our cross-sectional study (22). However, the longitudinal design brings a novel contribution to the existing literature. We found that substituting 5 min·d−1 of SB, LPA, or MPA for VPA at the age of 4.5 yr was associated with better score in handgrip strength test at 5.5 yr, which highlights the possible role of VPA instead of lighter PA in improving upper muscular strength. The results support our interpretation that the positive association between VPA and MVPA with BMI reflected higher fat-free mass instead of fat mass, given the strong association of FFMI with physical fitness in preschoolers (17). Bürgi et al. (3) has previously reported a positive association between baseline VPA and change in agility over 9-month follow-up. Thus, it seems reasonable to conclude that VPA and MVPA may predict later motor fitness in preschool-age children. Furthermore, there was a trend (P = 0.091) that substituting 5 min·d−1 of LPA for VPA at the age of 4.5 yr was related to a better score in motor fitness at 5.5 yr. This result may have occurred because VPA was not as strongly associated with motor fitness in the regression model analyses (β = −0.21) (the faster time the better score) as with lower muscular strength (β = 0.32) or cardiovascular fitness (β = 0.24).

The additional analyses showed that despite of the baseline PA level, increasing high-intensity PA between 4.5 and 5.5 yr was associated with improved body composition and muscular strength at follow-up. The results support our main findings and, additionally, emphasize the need to increase high-intensity PA to decrease %FM and FMI already at young ages.

The strength of our study is that we used accurate and up-to-date methodology for measuring preschool-age children’s body composition (15) and physical fitness (6) in a longitudinal design. Another strength is that we had accurate PA and SB data at baseline and at follow-up, enabling additional analyses to examine whether changes in PA and SB over the 12-month follow-up period were associated with corresponding changes in body composition and physical fitness. However, the study also has some limitations that need to be considered. First, the sample size of the study is somewhat small because to avoid biased results, we excluded the children who were in the intervention group. Nevertheless, the study was powered to detect weak associations, and despite the relatively small sample size, the associations found were strong and consistent. Second, it is an observational study, and thus, causality cannot be determined. Finally, because we wanted to compare our results to our cross-sectional study, we used cut points derived in 8- to 12-yr-olds to assess SB, LPA, MPA, and VPA. The cut points for wrist-worn wGT3x-BT in preschool-age children (21) are only for SB and MVPA. It is also notable that in our previous study (22), we analyzed accelerometer data using the cut points and the VM percentiles, and the results were consistent. In addition, the findings of our previous cross-sectional study have been recently confirmed by Collings et al. (9). Therefore, it is unlikely that the cut points we used have affected our results.

The MINISTOP study is strengthened by the fact that the participants were recruited from a population-based sample. As reported previously (13), no major difference in the child’s country of birth, sex, or area of residence between the MINISTOP sample and the whole invited sample were found. Thus, it is unlikely that our study suffers from any severe selection bias at the recruitment level. In addition, the participating parents’ BMI were in accordance with the general population (37), and the children’s body size and prevalence of overweight as well as intakes of fruits and vegetables, sweetened beverages, and candy were comparable to Swedish national data (27,31). The parents of participating children were slightly better educated than the general Swedish population (37), which may in turn limit generalizability of the results because of the association between high parental education and a higher performance in motor testing in preschoolers (1). However, in this context, it is relevant to note that our associations remained after adjustment for parental education.

The public health and clinical significance of the findings also deserves some comments. Overall, we identified quite strong associations of PA with body composition and physical fitness. For instance, a 5-min·d−1 greater VPA at the age of 4.5 yr was associated with a higher FFMI (β = 0.33), and with a better performance in the 20-m shuttle run (β = 0.24), standing long jump (β = 0.32), and 4 × 10-m shuttle run tests (β = −0.21) at 5.5 yr. Hence, we believe that the identified associations are strong enough to have importance for public health and possibly also for clinical care. These findings emphasize a potential role of high-intensity PA in preschool-age children in supporting fat-free mass and physical fitness in the long term. Therefore, games or structured PA that include short- and high-intensity bursts may be effective in increasing high-intensity PA in this age-group.

In conclusion, greater VPA and MVPA at the age of 4.5 yr were associated with higher FFMI and with better physical fitness at the 12-month follow-up. Greater VPA was also positively associated with BMI, and this finding reflected a higher fat-free mass (not fat mass). These observational results suggest that promoting high-intensity PA at young ages may have long-term effects by improving body composition and physical fitness, in particular muscular strength.

The authors thank the participating families as well as Eva Flinke Carlsson, Gunilla Hennermark, Birgitta Jensen, and Ann-Sofie Risinger for their help regarding recruitment and data collection, and Jani Raitanen for his help with the layout of the figure. The results of the present study do not constitute endorsement by the American College of Sports Medicine, and the results are presented clearly, honestly, and without fabrication, falsification, or inappropriate data manipulation.

The MINISTOP project was funded by the Swedish Research Council (project no. 2012–2883, M. L.), the Swedish Research Council for Health, Working Life and Welfare (2012–0906, M. L.), Bo and Vera Axson Johnsons Foundation, and Karolinska Institutet (M. L.). M. H. L. was supported by a grant from Juho Vainio Foundation and University of Jyvaskyla; P. H. was supported by a grant from Henning and Johan Throne-Holst Foundation; C. D. N. was supported by a grant from the Swedish Nutrition Foundation; H. H. was supported by grants from the Swedish Society of Medicine and the County Council of Östergötland, Sweden; F. B. O. and C. C.-S. were supported by the Spanish Ministry of Economy and Competitiveness (grant nos. RYC-2011-09011 and BES-2014-068829, respectively). None of the authors had a conflict of interest.

M. L. is the principal investigator for the MINISTOP trial and designed this research together with all coauthors. J. R. R., F. B. O., and C. C.-S. designed the fitness tests for the MINISTOP trial. C. D. N. was responsible for data collection, J. P. was responsible for processing of the accelerometer data, and P. H. was responsible for the statistical analyses and contributed to manuscript preparation. M. H. L. was responsible for data analysis and drafted the manuscript, which was subsequently reviewed by P. H., C. D. N., H. H., F. B. O., J. R. R., C. C.-S., J. P., and M. L. All authors approved the final version.

Trial registration: clinical trial (https://clinicaltrials.gov/: NCT02021786).

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    Keywords:

    EXERCISE; SEDENTARY BEHAVIOR; OBESITY; CARDIORESPIRATORY FITNESS; MUSCULAR STRENGTH; PEDIATRICS

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