F-58 Free Communication/Poster - Ergogenic Aids III Friday, June 2, 2017, 1:00 PM - 6:00 PM Room: Hall F
PURPOSE: Studies which evaluated the Heteropterys Tomentosa A. Juss extract (EHHt)-induced mutagenesis and its effects on swim performance in the animal models are non-existent. Thus, we verified, for the first time, the mutagenic potential (in vitro) as well as the acute ergogenic activity of the EHHt (in rats).
METHODS: Step one: CHO-k1 cells were used for in vitro micronucleus (MN) assay; these cells were treated with vehicle, 10, 30, and 100 mg/kg of EHHt; DOXO at 0.03M was used as positive control. Step two: Twenty-four male Wistar rats (90 days old; weight 180 to 220 grams) were divided into vehicle, EHHt 25mg/kg, EHHt 100 mg/kg, EHHt 400 mg/kg and subjected to swimming progressive load test (overload equivalent to 3% of body weight plus 0.5%, every 5 min, until exhaustion); caffeine at 5mg/kg was used as positive control.
RESULTS: Step one: The MN frequency was extremely lower (P=0.01) at 10 (5.33 ± 0.57), 30 (10.66 ± 4.04), and 100 (19.0 ± 1.41) mg/kg of EHHt if compared to vehicle (24.75 ± 5.18) and DOXO (90.7±3,1), being vehicle < DOXO. Step two: The rats administered with EHHt 25mg/kg presented higher values of time-to-exhaustion [TTE; min] (67.64±7.23; P=0.011) and exercise overload [EO; %] (9.6±0.96; P = 0.019) if compared to vehicle (TTE: 46.28±6.30; EO: 7.3±0.75), EHHt 100 mg/kg (TTE: 51.49±7.8; EO: 7.9±0.96), EHHt 400 mg/kg (TTE: 53.86±7.91; EO: 8.1±0.74), and caffeine (TTE: 59.17±9.43; EO: 8.6±0.89).
CONCLUSIONS: EHHt acutely improved the performance of the animals subjected to swimming exercise until exhaustion at 25 mg/kg dose and, importantly, showed a strong antimutagenic effect at all doses administered. Further studies are needed in order to better understand the bioavailability of EHHt and which substance(s), in isolation, is(are) the main inductor(s) of these two interesting outcomes and the possible correlation between then.