F-35 Free Communication/Poster - Nutrition and Chronic Disease Friday, June 3, 2016, 1: 00 PM - 6: 00 PM Room: Exhibit Hall A/B
Current guidelines for non-alcoholic fatty liver disease (NAFLD) treatment recommend a restricted diet plus physical activity. However, the underlying mechanisms are not clear.
PURPOSE: To investigate the effect of exercise training (EX) plus hypocaloric diet on NAFLD in a high fat diet (HFD)-induced obese mice.
METHOD: Fifty mice (C57BL/6) were assigned to standard chow (SC, n=10) or HFD (n=40) for 23 weeks. During the last 8 weeks of the 23-week dietary course, the HFD mice were further assigned to HFD (n=10) or HFD plus exercise training (HFD+EX) (n=10) or HFD+SC (n=10) or HFD+EX+SC (n=10). The HFD+EX and HFD+SC+EX mice were subjected to treadmill running at a moderate intensity for 50 minutes per session with a frequency of 5 days per week.
RESULT: Compared to the HFD mice, the HFD+EX, HFD+SC, and HFD+EX+SC mice had significantly lower values in areas under the curves of glucose (HFD: 49230±11353; HFD+EX: 45401±3357; HFD+SC: 25320±4321; HFD+EX+SC: 24466±2335) and insulin (HFD: 6225±1629; HFD+EX: 5871±1070; HFD+SC: 4466±570; HFD+EX+SC: 3784±254) tolerance tests and histological progression of hepatic steatosis (HFD: 3.8±0.4; HFD+EX: 2.5±0.5; HFD+SC: 2.3±0.5; HFD+EX+SC: 0.8±0.4) in conjunction with significantly higher levels of serum adiponectin (HFD: 23.0±1.8; HFD+EX: 29.5±4.4; HFD+SC: 31.6±3.4; HFD+EX+SC: 36.8±7.0) and hepatic adiponectin receptor 1 and 2 mRNAs. Attenuation of whole body insulin resistance (P=0.001 and P=0.001, respectively) hepatic steatosis (P=0.001, respectively), and hypoadiponectinemia (P=0.001, respectively) was significantly higher in the HFD+SC+EX mice than in the HFD+EX or HFD+SC mice. Compared to the HFD mice, the HFD+SC and HFD+EX+SC mice had significantly higher levels of hepatic genes (i.e., Tfam, NRF, COX4i1, SIRT1, CPT-1a, and CYP4a10 mRNAs) involved in mitochondrial function. Compared to the HFD mice, the HFD+EX, HFD+SC, and HFD+EX+SC mice had significantly lower levels of hepatic genes involved in inflammation (i.e., Ly6d and Lgals3 mRNAs) and fibrosis (i.e., Timp1 and Col1a1 mRNAs).
CONCLUSION: The current findings suggest that CR+EX has greater effects against NAFLD and its metabolic complications in HFD-induced obese mice.
Supported by the National Research Foundation Grant funded by the Korean Government (NRF-2012R1A1A2006180).