D-26 Free Communication/Poster - Carbohydrate Metabolism in Health/Disease Thursday, June 2, 2016, 1:00 PM - 6:00 PM Room: Exhibit Hall A/B
Carbohydrate ingestion may induce oxidative stress, inflammation, impaired insulin action, and altered substrate metabolism in a way that may counteract exercise-induced improvements in insulin sensitivity and metabolic flexibility. Benefits from exercise may differ in individuals with hypertriglyceredemia (hyperTG) compared to individuals with normal TG (normTG). HyperTG and sugar ingestion are linked to metabolic disease.
PURPOSE: Compare the triglyceride (TG), glucose (Glu), insulin (Ins), and C-peptide (CPEP) responses between exercise bouts with sugar sweetened beverage consumption (EX+SSB) verses water only (EX+H2O).
METHODS: Overweight and obese men (n=9) and women (n=11) (18-55 years) with fasting TG > (hyperTG) or < (normTG) 150 mg·dl-1 completed exercise. Each exercise was a 45-minute 10% grade uphill walk at 65% predicted VO2max. A standard, isocaloric diet to meet energy needs was given post exercise in the EX+H2O condition, but SSB replaced 2 g per kg lean mass of calories from food in the EX+SSB condition. An oral glucose tolerance test (OGTT) was completed the next day. Blood samples were collected pre-, 15, 30, 45, 60, 90, and 120-minutes post-OGTT for analysis of TG, Glu, Ins, and CPEP. Respiratory exchange ratio (RER) was measured collecting expired gas.
RESULTS: The release of insulin, as measured by CPEP from 0 to 30 min of the OGTT, was lower (p=0.04) while fasting TG were higher (p=0.03) for the EX+SSB condition (mean±SEM; 178.5±22.5 mg·dl-1) compared to the EX+H2O condition (148.4±14.1 mg·dl-1). Glucose area under the curve was higher (p<0.001) in the normTG group compared to the hyperTG group in the EX+H2O condition. Resting RER was lower (p=0.05) in the normTG group (0.74±0.03 VCO2/VO2) compared to the hyperTG group (0.78±0.04 VCO2/VO2) in the EX+H2O condition.
CONCLUSION: Consuming sugar with exercise was followed by higher fasting TG and lower Glu and Ins responses compared to EX+H2O during the OGTT. Fat utilization and metabolic flexibility during the OGTT were lower in the hyperTG group compared to the normTG group. Sugar consumption affected post-exercise metabolism more negatively in individuals with hyperTG.
Supported by the Mountain West Clinical Translational Research - Infrastructure Network under a grant from NIGMS of the NIH under Award Number 1U54GM104944.