A-45 Free Communication/Poster - Exercise Immunology Wednesday, June 1, 2016, 7: 30 AM - 12: 30 PM Room: Exhibit Hall A/B
Vaccination against infectious diseases has been one of the most successful public health interventions and several research have shown that acute eccentric exercise augments the antibody response to vaccination in humans. However, the underlying mechanisms are unclear and animal models are useful to understand the purported eccentric exercise-induced augmentation of antibody responses to vaccination.
PURPOSE: To determine if eccentric exercise could improve primary antibody responses to a suboptimal vaccination dose in mice.
METHODS: In the first experiment, mice were exercised at 17m/min speed at -20% grade for 60 minutes on a treadmill (ECC1) or remained sedentary (SED). Both ECC1 and SED mice were intramuscularly injected with 25μg of ovalbumin (OVA) and 200μg of alum adjuvant 6 hours post-exercise. In the second experiment, two bouts of downhill treadmill running were performed on consecutive days (ECC2) and all mice were vaccinated immediately after the second bout of exercise. In the third experiment, mice were randomly assigned to an eccentric electrically-stimulated group (ECCstim) or a sham group (Sham). Mice were then vaccinated 6 hours post-exercise. In all experiments, plasma was collected prior to, and at one, two and four weeks post-vaccination. ELISA was performed to analyze anti-OVA IgG.
RESULTS: In all three experiments, there was a significant time main effect indicating plasma anti-OVA IgG was significantly increased at one, two and four weeks relative to pre-immunization. However, there were no significant differences between ECC1, ECC2 or ECCstim and respective control groups, demonstrating that acute eccentric exercise did not further enhance the vaccine responses.
CONCLUSION: Acute eccentric exercise does not improve primary antibody responses in an animal model.