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Abst D-FreeCommPosters

Medicine & Science in Sports & Exercise: May 2012 - Volume 44 - Issue 5S - p 267–958
doi: 10.1249/01.mss.0000417529.22755.ed
Abstracts

A-29 Exercise is Medicine/Poster - Body Composition, Diabetes, Cancer, Disease Management

May 30, 2012 7:30 AM - 12:30 PM

ROOM: Exhibit Hall

1220 Board #1 May 30 9:30 AM - 11:00 AM

Sitting Time Was Highly Associated With Physical Activity Level And Obesity In Brazilian Public Employees

Luiz Guilherme G. Porto1, João Luis A. E. S. P. Leitão2, Évelin D. R. dos Santos2, Felipe F. Cavalcante2, Allan R. Mello2, Rayane G. A. Costa2, Leiriane V. Gregório2. 1 Brazilian Supreme Labor Court Health Service and University of Brasilia Faculty of Medicine - Cardiovascular Laboratory, Brasilia, Brazil. 2 Brazilian Supreme Labor Court Health Service, Brasilia, Brazil.

(No relationships reported)

Public health recommendations for physical activity (PA) have focused on the minimal PA that everyone should accumulate on a regular basis. Recently, studies have shown that sedentary behavior, as sitting time, could also be important in the public health context.

PURPOSE: To analyze the association between sitting time, PA level and body composition among Brazilian civil servants from the Superior Labour Court (TST).

METHODS: A cross-sectional study was done with a sample of 404 TST employees (189 women - 46.8%), aged 42.1±10.1 yrs and BMI: 26.0±4,3 kg/m2, randomly recruited and stratified by sex. Sitting time (ST) was assessed by questionnaire as proposed by Katzmarzyk et al (Med Sci Sports Exerc 41(5)), with data grouped in 2 categories: those who reported Longer ST Period (≥3/4 of day time - LSTP) and those who reported a Shorter ST Period (≤1/2 of day time - SSTP). PA level was characterized by the IPAQ Questionnaire, considering 2 PA categories: Insufficient Active group (IA - those who didn’t met PA recommendations) and Active group (AT - those who met PA recommendations). Body composition was analyzed by BMI and obesity was considered when BMI≥30 kg/m2. Chi-square (X2) was used to analyze variable associations and Odds Ratio (95% CI) (OR) was calculated to evaluate the strength of the associations. Differences were considered statistically significant when p<0.05.

RESULTS: 181 volunteers (44.8%) reported a LSTP, 193 (47.8%) were IA and 68 (16.8%) were obese. ST frequencies and association analysis are shown on Table 1.

CONCLUSIONS: We observed a negative association between ST and PA level as for the body composition. Volunteers who reported a longer ST period showed a higher risk (estimated by OR) of been insufficiently active and obese.

Table 1

Table 1

1221 Board #2 May 30 9:30 AM - 11:00 AM

Body Mass Index of North American Participants at the World Masters Games

Mark DeBeliso1, Joe Walsh2, Kent J. Adams, FACSM3, Mike Climstein, FACSM4, Ian T. Heazlewood5, Stephen Burke2, Jyrki Kettunen6. 1 Southern Utah University, Cedar City, UT. 2 Australian Catholic University, Sydney, Australia. 3 California State University Monterey Bay, Seaside, CA. 4 Bond University, Gold Coast, Australia. 5 Charles Darwin University, Darwin, Australia. 6 Arcada University, Invalidisäätiö, Finland.

(No relationships reported)

World Masters Games (WMG) athletes have either pursued a physically active lifestyle for an extended period of time or have initiated exercise/sport in later life. This unique cohort of middle- to older-aged adults remains relatively uninvestigated with regards to various measures of health. With a need for multifaceted solutions to the obesity epidemic, investigating special populations such as those competing in sport at older ages may further the understanding of the nexus between aging, physical activity and obesity.

PURPOSE: To investigate the body mass index (BMI) of North American WMG competitors with respect to national health guidelines and demographics.

METHODS: An online survey was utilized to collect demographic information from athletes competing at the Sydney WMG. BMI was derived using the participant’s height and body mass.

RESULTS: A total of 928 (46.7% male, 53.3% female) participants from Canada and the United States (age 52.6±9.8 yrs, range 27 to 87 yrs) completed the survey. The top 5 sports in which participants competed were football (25.6%), track/field (15.4%), swimming (8.4%), volleyball (8.2%), and softball (7.8%). Female and male BMI (kg/m2) across all sports were: >30 (obese-13.9%), 25-29.9 (overweight-34.1%), 18.5-24.9 (normal-50.3%), and <18.5 (underweight-1.7%). Data indicated that BMI was a health risk factor for 13.9% of the participants and a developing risk factor for 34.1% of the participants. Analysis demonstrated a significantly reduced (p<0.001) classification of obesity of the North American WMG competitors when compared to summary statistical values for the Canadian and United States national populations.

CONCLUSIONS: It is believed that adherence to exercise improves indices of general health. A key index of health (obesity) is significantly lower in incidence for North American WMG competitors when compared to Canadian and US populations.

1222 Board #3 May 30 9:30 AM - 11:00 AM

The Health Benefits of Exercise are Independent of Weight Loss in Obese Men and Women.

Phillipa Caudwell1, Catherine Gibbons1, Mark Hopkins1, Neil King2, Erik Naslund3, Graham Finlayson1, John Blundell1. 1 University of Leeds, Leeds, United Kingdom. 2 Queensland University of Technology, Brisbane, Australia. 3 Karolinska Institute, Stockholm, Sweden.

(No relationships reported)

Exercise is often prescribed as a method of weight loss and weight management, whilst other health benefits of exercise are less emphasized. Weight loss is not the only benefit of exercise and may not be the most appropriate marker of health.

PURPOSE: To examine the relationship between weight change and changes in health parameters during a supervised exercise program.

METHODS: METHODS: 107 overweight and obese men (n=35) and women (n=72) with a mean BMI=31.4±4.2kg/m2 and age= 40.9±9.2years, completed a supervised 12 week exercise program designed to increase gross energy expenditure (EE) by 2500kcal/wk at an intensity of 70% of their maximum heart rate. Various health markers including blood pressure (BP), aerobic fitness (AF), resting heart rate (RHR), and body composition were measured at weeks 0 and 12.

RESULTS: There was a significant reduction in mean body mass (-3.2±3.2kg, p<0.0001), however, there was large individual variability (range: -14.7 - +3.7kg) and approximately half (n=59) failed to change body composition as predicted - body mass (-0.81±2.11kg) and fat mass (-1.3±2.1kg). Despite this, their mean improvement in measured health markers was statistically significant; AF (+5.6±7.2ml/kg/min-1, p<0.0001) and fat free mass (+0.56±1.3kg, p=0.002) significantly increased, while RHR (-3.7±7.5bpm), Diastolic BP (-2.4±6.6mmHg, p=0.008), waist circumference (-3.2±2.7cm, p<0.00001) and % fat mass (-1.4±1.8, p<0.0001) significantly decreased. Improvements in blood pressure changes were more marked when hypertensive (135/90mmHg) individuals were examined separately. This group became normo-tensive by reductions in systolic (-13.0±10.1mmHg) and diastolic (-8.3±5.9mmHg) BP, which were independent of weight loss.

CONCLUSIONS: These data demonstrate that significant and meaningful exercise-induced health benefits can be achieved even with little or no weight loss. Therefore, the current public health focus on weight loss should be reduced and more attention focussed on improving general health. Supported by: Biotechnology and Biological Sciences Research Council (BB51/B/05079 and BB/G005524/1).:

1223 Board #4 May 30 9:30 AM - 11:00 AM

Minimal Changes in Breathing Mechanics at Peak Exercise in Endurance-trained Obese Individuals

Santiago Lorenzo1, J. Todd Bassett2, Raksa B. Moran2, Jessica N. Pineda2, Tony G. Babb, FACSM3. 1 Texas Health Presbyterian Hospital Dallas and UT Southwestern Medical Center, Dallas, TX. 2 Texas Health Presbyterian Hospital Dallas, Dallas, TX. 3 UT Southwestern Medical Center, Dallas, TX.

(No relationships reported)

PURPOSE: Alterations in respiratory mechanics predisposes healthy obese individuals to low lung volume breathing at rest and during exercise, which places them at risk of developing expiratory flow limitation (EFL). The high ventilatory demand in endurance-trained obese adults further increases their risk of developing EFL as well as their work of breathing. Therefore, we measured breathing mechanics and ventilatory dynamics during exercise in endurance trained obese individuals.

METHODS: Seven (5W/2M) endurance-trained obese adults (35±3yr, 37±5%body fat, 33±4 BMI, mean±SD) underwent hydrostatic weighing, pulmonary function testing, submaximal and maximal cycle exercise testing, and the determination of the oxygen cost of breathing during eucapnic voluntary hyperpnea (EVH).

RESULTS: Pulmonary function was normal (FVC 109±14%predicted, FEV1 108±11%predicted, PEF 119±13%predicted, MVV 129±6%predicted, TLC 101±13%predicted, FRC 45±6%TLC). Peak VO2 and work rate were 135±16 and 133±9%predicted, respectively. Peak ventilation, tidal volume, and breathing frequency were 125±36L/min (77±11%MVV), 2.6±0.6L (56±5%FVC), and 49±8bpm, respectively. During submaximal exercise end-expiratory lung volume dropped slightly but it returned to resting levels at peak exercise (rest 41.5±5.7, submaximal 38.1±8.5, peak 42.0±7.2%TLC). Slight EFL at peak (16±5% of tidal volume) without desaturation (98±1%) was observed in 6 subjects (5W/1M). During submaximal exercise the ventilatory response (VE/VCO2 slope) was 25±1, and at peak exercise the rating of perceived breathlessness (Borg 0-10 scale) was 7.7±1.6. The estimated oxygen cost of breathing during EVH was 1.75±0.74ml/L and during peak exercise averaged 7±3% of peak VO2.

CONCLUSIONS: Fit obese individuals were able to adequately increase ventilation and match the elevated metabolic demand associated with high fitness without substantial alterations in breathing mechanics. We conclude that endurance-trained obese individuals with normal lung function are not limited by breathing mechanics, which is good news for otherwise healthy obese adults who wish to participate in vigorous exercise training.

1224 Board #5 May 30 9:30 AM - 11:00 AM

Fat Free Mass Response Of Overweight Males Following 12 Weeks Of High-intensity Intermittent Exercise

Mehrdad Heydari1, Judith Freund2, Stephen H. Boutcher, FACSM1. 1 University of New South Wales, Sydney, Australia. 2 St Vincent’s Hospital, Sydney, Australia. (Sponsor: Stephen Boutcher, FACSM)

(No relationships reported)

The effect of high-intensity intermittent exercise (HIIE) on fat free mass (FFM) of overweight males is undetermined.

PURPOSE: To examine the effect of a 12-week HIIE intervention on FFM levels of sedentary overweight men.

METHODS: Thirty eight males aged 24.9±4.3 years, with a BMI of 28.7±3.1 kg/m2, and body mass of 88.4±11.9 kg underwent a VO2max test pre- and posttraining. Total body fat and FFM was assessed by dual energy x-ray absorptiometry. Subjects, who were randomly assigned to HIIE (n=20) or control groups (n=18), exercised three times a week for 12 weeks under supervision. The HIIE group completed 20 minutes of exercise (8 s sprint, 12 s recovery) each session, whereas controls performed no structured exercise for 12 weeks. Dietary intake was monitored through food diaries.

RESULTS: VO2max was significantly improved, P<0.05, by an increase of 15% in the HIIE group (34.2±4.4 to 39.4±3.5 ml.kg-1min-1) and remained unchanged in the control group. Total FFM was significantly increased, P<0.05, in the exercise group by 1.2 kg (54.3±6.5 to 55.5±6.1 kg) and remained unchanged in controls (53.8±6.5 to 54.2±6.1 kg). FFM increased by 0.4 kg in the leg (18.6±2.5 to 19.0±2.4 kg) and 0.7 kg in the trunk (24.9±3.1 to 25.6±3.0 kg) and was unchanged in the arm. Total fat mass in the HIIE group was significantly reduced (29.8±7.1 to 27.8±6.9 kg; P<0.05) by 7%, whereas in the controls fat mass was unchanged (31.7±9.3 to 31.8±9.9 kg).

CONCLUSIONS: Twenty minutes of HIIE, three times a week for 12 weeks, led to a significant decrease in fat mass and a significant increase in FFM in overweight, young males.

1225 Board #6 May 30 9:30 AM - 11:00 AM

A Multidisciplinary Intervention Results in Sustained Improvements in Fitness and Metabolic Profile in Overweight Youth

Karissa L. Peyer1, R Randall Clark1, Jens Eickhoff2, Aaron L. Carrel2. 1 University of Wisconsin Hospital and Clinics, Madison, WI. 2 University of Wisconsin School of Medicine and Public Health, Madison, WI.

(No relationships reported)

PURPOSE: To evaluate the impact of a clinical intervention on body composition, aerobic fitness, cholesterol, and insulin sensitivity in a clinic for overweight children.

METHODS: 79 children (age 10.56 ± 3.24 years, BMI z-score 1.15-3.48) were followed for 24 months at a Pediatric fitness clinic. All children were seen for initial visit (T0), at 12 months (T12), 24 months (T24), and often for addition follow-up visits between these time points. All children had height, weight, and blood pressure assessed and BMI z-score was computed. DXA and a submaximal treadmill test were used to assess body composition and aerobic fitness. Many children also had carbohydrate and lipid metabolism assessed. Children and parents attended appointments with physicians, registered dieticians, and exercise physiologists to assess lifestyle habits and establish goals for healthy change. Follow-up intervals were set on an individual basis and typically ranged from 6 weeks to 3 months. Changes from baseline were evaluated using the nonparametric Wilcoxon Signed Rank test. Spearman’s rank correlations were also performed to assess relationships between variables.

RESULTS: At T12, significant changes (mean ± SD) were seen in VO2max (1.39 ± 3.25 ml/kg/min, p = 0.001), %fat (-2.18 ± 2.79%, p < 0.001), BMI z-score (-0.09 ± 0.21, p < 0.001), and Total Cholesterol (-19.72 ± 44.42 mg/dL, p = 0.01). There were no significant changes in fasting glucose, insulin or blood pressure. There were no significant differences between genders. All improvements remained significant at T24 [VO2max (1.32 ± 3.98 ml/kg/min, p = 0.006), %fat (-1.25 ± 2.87%, p = 0.006), BMI z-score (-0.13 ± 0.28, p < 0.001), and Total Cholesterol (-11.82 ± 22.77mg/dL, 0.025)]. Correlation analysis revealed a significant negative correlation (p<0.001) between changes in BMI z-score and VO2max levels from baseline to T12 (r = -0.67) and T24 (r = -0.71).

CONCLUSIONS: A personalized, multidisciplinary intervention focusing on lifestyle changes in nutrition and exercise for children yielded significant improvements in cholesterol measures, aerobic fitness, body composition and BMI for 2 years. Children who displayed positive changes in BMI z-score also saw the greatest improvement in VO2max. Further research should evaluate outcomes at longer follow-up intervals.

1226 Board #7 May 30 9:30 AM - 11:00 AM

Body Mass Index and Parental Efficacy for Children’s Exercise within a Youth Weight Management Program

David Bellar1, Charity L. Bryan1, Lisa Broussard1, Holly Howat1, Lawrence W. Judge2. 1 University of Louisiana at Lafayette, Lafayette, LA. 2 Ball State University, Muncie, IN.

(No relationships reported)

Childhood obesity is an alarming trend within the United States and other areas of the world. Greater understanding of the predictors of childhood obesity and overweight are necessary to effectively combat this growing problem.

PURPOSE: The present investigation was designed assess the efficacy of parental provision of exercise for children and its relationship to health and fitness in a group of overweight and obese youth.

METHODS: Fourteen parents and children (Male n=5, Female n=9, Age: 11.0±2.0yrs, Height 152.5±12.3cm, BMI 34.75±10.3) volunteered to participate in the present investigation. The participants were selected from the population of a local youth weight management program. After consent and assent were collected, the parents were asked to fill out a modified version of the Self Efficacy Scale for Exercise. The youth were tested for cardiovascular fitness via a progressive 20 meter shuttle run, body composition via two site skinfold testing (calf and tricep), body mass index, muscular endurance via curl up and push up tests, and flexibility through back saver sit and reach as well as trunk lift and shoulder flexibility testing.

RESULTS: In general the youth in the study were classified in the healthy fitness zone for the flexibility assessments, however, for all body mass or body composition assessments and tests of muscular endurance or aerobic capacity participating youth were below the healthy fitness zone. There was a significant negative correlation between the reported efficacy of provision of exercise by the parents and the body mass index of the children (r=-0.522, p=0.05).

CONCLUSIONS: The results of this study suggest that parental efficacy may be a discriminating factor within a population of overweight and obese, low fitness level youth. As such, parental empowerment may be a key to health related fitness within this population.

1227 Board #8 May 30 9:30 AM - 11:00 AM

Effects Of Family-based Behavioral Group Therapy And Exercise Training In Pre-pubertal Obese Children And Their Mother

Nathalie J. Farpour-Lambert1, Albane M. Maggio1, Xavier E. Martin1, Sophie Bucher Della Torre2, Yacine Aggoun1, Maurice Beghetti1. 1 University Hospital of Geneva, Geneva 14 , Switzerland. 2 School of Dietetics HES, Geneva, Switzerland.

(No relationships reported)

PURPOSE: To determine the effects of a family-based behavioral group therapy combined with an exercise training program on body fat and cardio-respiratory fitness in pre-pubertal obese children and their mother.

METHODS: This was a 6-month RCT including 50 pre-pubertal obese children (mean age 9.7 years, 95% CI 9.1-10.1) and their mother (40.5 years, 38.2-43.0) were randomly assigned to an Intervention (I, n=25) or a Control group (C, n=25). The intervention consisted of 14 behavioral group therapy sessions (nutrition, physical activity, body image, motivation, positive reinforcement, social and parental skills) once a week for children and mothers separately. In addition, children exercised (swimming, ball games, resistance training) one hour twice a week, and mothers participated to a one-hour Nordic walking session per week. We assessed changes at 6 months in BMI, waist circumference (WC), total body and abdominal fat by dual-energy x-ray absorptiometry (DXA), and cardio-respiratory fitness (VO2max).

RESULTS: At baseline, children’s characteristics were similar among groups. Mothers in the Intervention group had significantly (p<.05) higher WC, total body and abdominal fat than Controls. At 6 months, using mixed linear regression, we observed significant changes in children’s BMI (I-C treatment effect -0.67 g.cm-2), BMI Z-score (-0.15), WC (-1.33 cm), total body fat (-0.94%), abdominal fat (-2.08%), and VO2max (83.7 ml.min-1). In mothers, significant changes in BMI (I-C treatment effect -0.85 kg.cm-2), WC (-9.24 cm), total body fat -0.61%), and abdominal fat -1.43%) were shown.Changes in VO2max were not significant in mothers.

CONCLUSIONS: Family-based behavioral therapy combined with exercise training significantly reduces BMI and body fat in both pre-pubertal obese children and their mother. This is an interesting public health approach to promote behavioral changes and reduce the degree of adiposity at the family level. This study was funded by the Swiss National Science Foundation (#3200B0-120437) and the Geneva University Hospital Research and Development Fund.

1228 Board #9 May 30 9:30 AM - 11:00 AM

Examination of Modifiable Risk Factors for Obesity-Related Disease in African American College Students

Brenda L. Swearingin1, Jamie E. Robbins2, Patricia A. Lynch1, Wi-Young So3, Brandon J. Crooms1. 1 North Carolina A&T State University, Greensboro, NC. 2 Winston Salem State University, Winston Salem, NC. 3 Seoul Women’s University, Seoul, Korea, Republic of.

(No relationships reported)

The disparity in the prevalence of obesity and concomitant risk for cardiovascular disease in African Americans (AA) is a significant medical concern. Obesity-related chronic disease, associated with modifiable behaviors, is being diagnosed in younger AA populations.

PURPOSE: To examine relationships of the following variables in AA college students: 1) social support for healthy behaviors, 2) amount of physical activity (PA), 3) diet, 4) body mass index (BMI), 5) systolic (SBP) and diastolic (DBP) blood pressure, and 6) waist circumference (WC).

METHODS: 412 AA students completed a physical exam (BMI, SBP, DBP and WC) and a packet of surveys including the social support survey (SOC), a fruit and vegetable screener (FVS), a fat screener survey (FS), and the Paffenbarger PA questionnaire. Relationships were analyzed using one-way ANOVA, tukey post hoc comparisons and logistical regression.

RESULTS: The prevalence of overweight (OVW) and obesity (OB) was 28.3% and 30.3% respectively in males (N= 198, 20+1.5yrs) and 23.0% and 26.7% respectively in females (N=217, 19.8+1.6 yrs). In males, OVW subjects had WC (p < 0.01) and SBP (p < 0.05) indicating risk for cardiovascular disease (CVD) and low PA for recreational activities (p < 0.05). The OB group was classified as increased risk for WC (p < 0.001), SBP (p < 0.001), and DBP (p < 0.001) and lower than normal recreational activities (p < 0.05). Female subjects in the OVW group met risk classification criteria for WC (p < 0.001) and SBP (p < 0.05). Female subjects in the OB group had CVD risk classifications for WC (p < 0.001), SBP (p < 0.001), and DBP (p < 0.001) and lower than normal PA (p < 0.05). Logistic regression showed, for both males and females, there was no statistical significance between OVW or OB and SOC, PA, FVS, and FS for males or females. When PA was increased by 1,000 kcal, the prevalence of OB decreased by 9.3% (OR = 0.907, CI = 0.834-0.986, p = 0.022) in males and 9.0% (OR = 0.910, CI = 0.839-0.988, p = 0.025) in females.

CONCLUSIONS: The only significantly different risk factor for obesity in college African Americans is PA. Differences in female PA for normal vs. overweight/obesity existed in total PA; however, males demonstrated significant differences in PA for recreational activities alone. Supported by USDA Grant 0204191.

1229 Board #10 May 30 9:30 AM - 11:00 AM

Student Athletes Have A More Favorable Body Composition And Bone Mineral Density Than Non-athletes

Hattie H. Wright, Chrisna R. Botha, Lize Havemann-Nel. North-West University, Potchefstroom, South Africa.

(No relationships reported)

PURPOSE Data on the relative contribution of planned exercise to body composition and BMD of premenopausal black and white African women is scarce.

METHODS: Volunteer black and white University female students were recruited into groups according to exercise and race, namely white athletes (WA, n=117) and black athletes (BA, n=50), doing ≥5h/wk of planned weight bearing exercise, as well as white non-athletes (WNA, n=44) and black non-athletes (BNA, n=44) doing ≤1h/wk of planned exercise. Students with a body mass index (BMI) <28.0 and not pregnant were included. Exclusion criteria were use of corticosteroids/thiazides for ≥ 6 months and any known bone disease. Alpine spine (APS), femoral neck (FN), total hip (TH), and whole body (WB) BMD as well as body composition were measured with dual energy X-ray absorptiometry.

RESULTS: Mean age, weight, height, and BMI for the total group were 20.3±1.8yrs, 60.7±8.6kg, 1.65±0.1m, and 22.1±2.3kg/m2. WA were younger and heavier than both BNA and BA (p<0.05). Height differed significantly between all groups with WA the tallest and BNA the shortest [median = 1.67(1.64-1.72)m vs. 1.59(1.54-1.64)m, p<0.05]. After adjusting for age and weight BMD were similar between WNA and BNA, as well as WA and BA. WNA had lower BMD at all sites compared to WA (p<0.001). BNA had lower BMD at all sites compared to BA [APS= 0.969(0.912-1.028) vs. 1.087(0.991-1.148)g/cm2; FN=0.821(0.773-0.904) vs. 0.951(0.888-1.028)g/cm2; TH=0.926(0.872-1.012) vs. 1.028(0.959-1.099)g/cm2; and WB=1.072(1.003-1.108) vs. 1.129(1.074-1.180)g/cm2; p<0.001)]. Body fat percentage (BF%) was similar between WNA and BNA as well as WA and BA. WNA had significantly higher BF% than WA, and BNA than BA (p<0.05). Fat-free mass was similar between WNA and BNA, but lower in BNA compared to both WA and BA [38.3(33.5-42.0)kg vs. 46.4(43.3-49.1)kg and 42.3(39.9-46.7)kg, p<0.001). 36.4% WNA and 31.8% BNA had a Z-score between -1.0 and -2.0 at one or more BMD sites compared to 7.7% WA and 10% BA. Positive correlations were found between fat-free mass and height (r=0.7), weight (r=0.8), APS, FN, TH, and WB BMD (r=0.5 for all, p<0.05).

CONCLUSIONS: Exercise seemed to be the main contributing factor towards a higher BMD and a healthier body composition amongst this group of female students; ethnicity did not seem to play a role.

1230 Board #11 May 30 9:30 AM - 11:00 AM

Effect Of Tai Chi During Dietary Weight Loss On Body Composition In Obese, Postmenopausal Women

Lynn A. Katkowski, Marisa C. Benson, Steven Magnanti, Matthew J. Delmonico, Ingrid E. Lofgren, Furong Xu. University of Rhode Island, Kingston, RI.

(No relationships reported)

Obesity is a growing problem for the aging population. Obesity, particularly in older women, is associated with increased risk for chronic disease, disability, and loss of physical functioning. Tai Chi, a low impact form of exercise, has been shown to be effective in improving outcomes including strength and balance in older adults. However, there is no published research examining the effect of Tai Chi during dietary weight loss on body composition in obese older women.

PURPOSE: To examine the additive effect of Tai Chi during dietary weight loss on body composition in obese, postmenopausal women.

METHODS: A 16-week trial was conducted with 27 obese women randomized to a Tai Chi plus weight loss group (TCWL, n=14, age 60.4 ± 5.9 years, BMI = 34.3 ± 5.1 kg/m2) or a weight loss only group (WL, n=13, age 62.7 ± 6.0 years, BMI = 34.8 ± 2.9 kg/m2). Body mass, height, and waist circumference were assessed using standard techniques. Total body mass, fat mass, and fat free mass were assessed using dual-energy x-ray absorptiometry. Thigh tissue composition was assessed using computed tomography. Both groups received a Dietary Approaches to Stop Hypertension-based weight loss program. The TCWL group also participated in three Tai Chi exercise sessions (∼45 min) per week. Between-group comparisons were analyzed using an analysis of covariance adjusting for baseline values.

RESULTS: Both groups significantly lost body mass (TCWL, -2.2 ± 0.9 kg; WL, -3.7 ± 0.9 kg; both p < 0.05) with no between-group differences. The TCWL group had a borderline attenuation in fat-free mass change compared to the WL group (-0.2 kg vs.-1.2 kg, p = 0.056). There were significant between group differences in thigh high density muscle (-0.54 kg vs. -1.32 kg, p = 0.0311). There were no significant between group changes in BMI, total fat mass, percent body fat, waist circumference, fat area, normal density muscle, or muscle fat.

CONCLUSIONS: Tai Chi appears to aid in preservation of fat free mass but does not have significant additive effect on body fat measures during dietary weight loss in older, postmenopausal women.

1231 Board #12 May 30 9:30 AM - 11:00 AM

Effect Of Martial Arts Exercise On Body Composition, Bone Biomarkers, And Quality Of Life In Overweight Premenopausal Women

Ming-Chien Chyu1, Raul Y. Dagda2, Susan Doctolero2, Eugene Chaung2, Vera Von Bergen2, Yan Zhang2, Michael Ragain2, Jean-Michel Brismée2, Chwan-Li Shen2. 1 Texas Tech University, Lubbock, TX. 2 Texas Tech University Health Sciences Center, Lubbock, TX.

(No relationships reported)

INTRODUCTION: Various exercise interventions have been shown to benefit weight control and promote general health, including bone health. Bone mineral density at the spine and hips as well as bone formation markers were found to be significantly associated with the level of physical activity of healthy premenopausal women. However, there is no data on the effects of martial arts exercise (MAE) on body composition, bone biomarkers, and quality of life in overweight/obese premenopausal women.

PURPOSE: To evaluate the effects of 12 weeks of MAE intervention on body composition, bone biomarkers and quality of life in overweight/obese premenopausal women.

METHODS: After screening, qualified premenopausal women were randomized into two groups: MAE (1 hr/session, 3 sessions/week for 12 weeks) or control group. Body composition (Tanita Body Composition Monitor), serum biomarkers (osteocalcin, C-terminal telopeptide, insulin-like growth factor-I, leptin, and C-reactive protein), and quality of life (SF-36) were assessed at baseline, 6, and 12 weeks. Data were analyzed using paired t-tests.

RESULTS: Twenty-three subjects participated in the MAE group (40.2±5.7 yr, BMI: 36.0±5.4 kg/m2) and 24 in the control group (40.6±6.9 yr, BMI: 35.8±7.1 kg/m2). Fourteen subjects dropped out (7 in MAE, 7 in control) before completing the intervention due to issues such as family, job, personal, and schedule. Compared to the control group, MAE group tended to lose body weight (P=0.09) along with decreased fat-free mass (P=0.007) and muscle mass (P=0.022). The MAE group demonstrated an increase in serum insulin-like growth factor-I concentration (P=0.045), but no change in other serum biomarkers including osteocalcin (bone formation biomarker), C-terminal telopeptide (bone resorption biomarker), leptin and C-reactive protein. The results of SF-36 revealed that subjects in the MAE group reported a significant improvement in general health, vitality, role-emotional, and mental health as compared to those in the control group.

CONCLUSIONS: MAE may be a feasible, low-cost, and effective approach to improve body composition and quality of life in overweight/obese premenopausal women. Our study underscores the need for further long-term studies using larger sample size to establish the benefits of MAE in this/other populations.

1232 Board #13 May 30 9:30 AM - 11:00 AM

Exercise Vital Sign Correlates with Diabetes Control

Robert Sallis, FACSM1, Eunis Ngor2, Karen Coleman2. 1 Kaiser Permanente Medical Center, Fontana, CA. 2 Southern California Permanente Medical Group, Pasadena, CA.

(No relationships reported)

The Exercise is Medicine campaign calls for physicians to evaluate the exercise habits of their patients and Kaiser Permanente in Southern California (KPSC) was the first major healthcare system to begin using an exercise vital sign to record patient activity levels at every visit. Use of the “Exercise Vital Sign” (EVS) began in October of 2009, with every patient reporting the minutes per week of moderate or greater exercise they typically engage in. In addition, the role of physical activity in both the treatment and prevention of diabetes has been well established. It stands to reason that patients who report doing recommended amounts of regular exercise are likely to have improved control of their diabetes.

PURPOSE: To evaluate the correlation between self reported exercise level (using the EVS) and diabetes control as assessed by hemoglobin A1c (HbA1c) levels.

METHODS: Data were abstracted from the KPSC electronic medical records of 362,856 adults 18 years and older who had at least one HbA1c value during 4/1/2010 to 3/31/2011. Possion regression analyses were done to determine the association of the following predictors with HbA1c values >= 7.0: age, gender, race/ethnicity, body mass index (BMI), Charlson comorbidity index, and median exercise min/wk.

RESULTS: Independent of all other predictors including BMI and disease burden, adults who were completely sedentary (0 min/wk) had 16% increased risk of HbA1c >= 7.0 as compared to adults who were regularly active (150 min/wk; p < .001).

CONCLUSIONS: Based on data from the EVS, we conclude that self reported physical activity is strongly correlated with improved diabetes control. It is well known that improved diabetes control is a major factor in reducing complications and costs associated with diabetes. For this reason, it seems logical that all diabetic patients who report they are not doing any exercise should be targeted with interventions designed to increase their weekly levels of physical activity.

1233 Board #14 May 30 9:30 AM - 11:00 AM

Effect Of Exercise Training On Neuromuscular Function Of Arm And Leg Muscles Of Diabetic Patients

Massimo Sacchetti1, Ilenia Bazzucchi1, Francesco Felici1, Susan Dewhurst1, Antonio Sgadari2, Giuseppe De Vito1. 1 University of Rome Foro Italico, Rome, Italy. 2 Università Cattolica del Sacro Cuore, Rome, Italy. (Sponsor: Carl Foster, FACSM)

(No relationships reported)

Diabetes is associated to muscle weakness and physical disability. On the other hand, exercise training has the potential of counteracting the diabetes-associated derangement of neuromuscular function.

PURPOSE: To investigate the effect of exercise training on neuromuscular function of arm and leg muscles in type 2 diabetic patients.

METHODS: Eight type 2 sedentary diabetic patients (D, 61.0±2.3) and eight sedentary healthy matched control subjects (H, 63.9±3.8) underwent a 16-week supervised combined aerobic and resistance exercise training program. Before and after training, maximal isometric (MVC) and isokinetic (15, 30, 60, 120, 180 and 240°/s) muscle strength of the elbow flexors (EF) and knee extensors (KE) were assessed to investigate the torque-velocity relationship. Simultaneously, the EMG activity from the biceps brachii (BB) and the vastus lateralis (VL) muscles was recorded by means of array electrodes, in order to estimate the muscle fibre conduction velocity (MFCV).

RESULTS: After the training program, no significant changes in EF maximal torque values were noted in both groups. Differently, KE maximal torque values of D were significantly enhanced (p<0.05) during the MVC (+10,8%) and during isokinetic contractions at 15, 30 and 60 °s-1 (+17,1% on average). EMG data mirrored what found for torque, with an increase in MFCV in VL in D during MVC and during isokinetic contractions at 15 and 30 °s-1 (+ 11.2% on average; p<0.01).

CONCLUSIONS: Exercise training is more effective in KE than in EF muscles of diabetic patients, as shown by the shift toward higher values of the torque-velocity relationship and the enhancement of MFCV. This is in line with the more pronounced neuromuscular impairment of the lower than the upper extremities in type 2 diabetes.

1234 Board #15 May 30 9:30 AM - 11:00 AM

Compliance, Safety And Utility Of Smartphone Indicators During A 52 Week Randomized Trial Of Prescriptive Exercise In Rural Patients With Metabolic Syndrome

Robert J. Petrella, FACSM1, Melanie Stuckey1, Kristin Sabourin1, Sheree Shapiro1, Brittany Izanstadt2, John A. Petrella1, Claudio Munoz3, Robyn Fulkerson4. 1 U Western Ontario, London, ON, Canada. 2 U Waterloo, Waterloo, ON, Canada. 3 Gateway Rural Research Institute, Seaforth, ON, Canada. 4 Sykes Assistance Services, London, ON, Canada.

(No relationships reported)

PURPOSE: To determine whether remote clinical monitoring technologies can provide markers of intervention compliance, safety and efficacy outside the clinic setting.

METHODS: In a 52 week randomized trial of lifestyle intervention to modify risk factors for metabolic syndrome (Artemis), we used a Smartphone technology- intensive monitoring system linked to a central database in an underserviced rural setting. 75 participants were prescribed a lifestyle intervention and provided with Smartphones and Bluetooth-enabled blood pressure (BP) monitors, glucometers and pedometers. Smartphones transmitted pre-determined daily clinical data to a central database (Healthyanywhere™): BP twice daily; blood glucose (BG) twice daily and steps daily. A customized, proprietary decision support system (DSS) detected abnormal clinical data and life style patterns based on pre-determined clinical threshold algorithms. BP was grouped as normal, high-normal, pre-hypertensive, Stage 1, Stage 2; BG as normal, impaired glucose tolerance, diabetic; steps as sedentary, low active, somewhat active, active and highly active.

RESULTS: 68 participants (48 women) [56.8 (9.8)] years old completed the study and are reported 0-12, 13-24 and 25-52 weeks. No differences were observed for gender. Compliance with data transmission was high (81-94%) across all measures and did not change over time. Surprisingly, BP and BG were more compliant than daily steps transmission. Most clinical data were in the target therapeutic range over time. BP and BG highest 2 groups showed a decline (p<0.05) over the course of the study. 28-30% of patients achieved the target of 10,000 steps per day at each time point. % of patients in the sedentary, low and somewhat active groups declined with time (p<0.05).

CONCLUSIONS: These findings show that clinical data transmission compliance was high and few patients showed clinically relevant alerts during a 52 week lifestyle intervention. High threshold alerts decreased over time while more patients became active. Smartphone monitoring of lifestyle interventions appears to be an important tool for monitoring safety and utility of lifestyle interventions in primary care.

1235 Board #16 May 30 9:30 AM - 11:00 AM

The Reliability of Behavioral Modification (Diet and Physical Exercises) on Metabolic Syndrome Primary Care

Franz H P Burini1, Okesley Teixeira2, Fernando Moreto2, Erick P. de Oliveira2, Roberto C. Burini2. 1 USP - PRONUT, São Paulo, Brazil. 2 UNESP - Univ Estadual Paulista, Medical School, Botucatu, Brazil.

(No relationships reported)

There is no curative model for the Metabolic Syndrome (MetS), being the primary prevention, such as the combat of sedentary behavior and food-intake inadequacy (lifestyle changes) the aim of the primary care system.

PURPOSE: To evaluate the effects of a protocol including dietary counseling and supervised physical exercises (LSMP) on MetS components.

METHODS: The longitudinal study included 131 subjects (55 ± 9 yrs old, 74% female and 68.7% under 60 yrs old) from a community-based sample that fullfiled the ethical and inclusion criteria of attending the 24 weeks LSMP and to all evaluations. The LSMP included a daily (5×/wk) sessions of supervised aerobic exercises (80 min, 65-80% VO2max), weekly dietary counseling and assessments (baseline and end-study) of treadmill cardiorespiratory fitness (CRF, Balke protocol), dietary intake, anthropometry and blood chemistry. The ATP-NCEP (2003) criteria was used for the MetS diagnosis and the Statistica 6.0 software for the analyses of the data (p=0.05).

RESULTS: At baseline the MetS group (MSG=50.3%) differed from the non-MS (NMS) by the higher body mass index (BMI) and plasma uric acid (UA) and lower in CRF. Both groups responded to LSMP by increasing CRF, health-eating index, lowering the food-energy intake and plasma high sensitive C-Reactive Protein (CRP-hs). The 24.2% MetS reduction with LSMP were due to waist circumference (WC), blood pressure (BP) and HDL-c normalization whereas the appearance of 10.8% MS in the NMS group during the LSMP was associate with the increased levels of plasma glucose and triglycerides (TG) along with a decreased dietary-fiber intake. The predictive variables for the MetS control with LSMP were the higher increasing of HDL-c and the decreasing of BP whereas the predictive risk factors for the MetS appearance with LSMP were the increasing of BMI, plasma glucose, TG and UA, and the lower consumption of dietary fibre.

CONCLUSIONS: These data lead to the conclusion that the MetS treatment by LSMP is primarily due to de lower energy intake (by increasing fibre consumption) and/or lower insulin resistance (by increasing CRF). Supported by CNPq and CAPES

1236 Board #17 May 30 9:30 AM - 11:00 AM

Effect Of Regular Exercise Training On Cardiorespiratory Fitness In Diabetes Mellitus: Meta-analysis

Juan Wang1, Zhengzhen Wang1, Xianbo Zhang2. 1 Beijing Sports University, Beijing, China. 2 Peking University, Beijing, China.

(No relationships reported)

PURPOSE: Cardiorespiratory fitness is the core element of physical health, and it is not only closely related to the incidence but also directly associated with their mortality. Now there are many studies on exercise and Cardiorespiratory fitness in diabetes mellitus, but these studies had relatively small sample sizes and highly variable results. And few literature reviews focus on this. Therefore this article tries to find out the effect of regular exercise training on cardiorespiratory fitness in diabetes mellitus by using meta-analysis.

METHODS: Medline (1975 to Sep 2011) and SPORT Discus (1936 to Sep 2011) were searched for randomized, controlled trials evaluating effects of regular exercise interventions(not combined other intervention) of 4 weeks or more on cardiorespiratory fitness in diabetes mellitus. All satisfied trials were assessed by Jadad scales. And related data were extracted by two independent researchers. Then all the data were analyzed via software (Review Manage 5.0).

RESULTS: 1) there were 26 studies searched about exercise intervention on cardiorespiratory fitness in type 2 diabetes mellitus. Regular exercise can increase the VO2max about 14.1% in type 2 diabetes mellitus (SWD=1.01SD, p< .05). In the subgroups, Compared to the parallel controlled group, mellitus’s VO2max was different only in 60-79%VO2max intensity exercise group(p< .05) while not occurred in 40-59%VO2max or ≥80%VO2max intensity exercise group(p> .05). The VO2max of type 2 diabetes mellitus both increased in moderate exercise group and moderate plus resistance exercise group, and the former is more effective (p< .05); 2) there were only 4 studies satisfied the criteria in type 1 diabetes mellitus. After regular exercise, VO2max increased 8.6%, no significantly (SWD= 0.33SD, p> .05).

CONCLUSIONS: 1) for type 2 diabetes mellitus, regular exercise is benefit for improving cardiorespiratory fitness, both moderate exercise and moderate plus resistance exercise can, and it’s sure about 60-79%VO2max intensity exercise can improve the VO2max. 2) It’s not sure about regular exercise can improve cardiorespiratory fitness in type 1 diabetes mellitus.

1237 Board #18 May 30 9:30 AM - 11:00 AM

Additional Benefits of W-3 Oil Supplements on Metabolic Syndrome Care in Exercise Protocol Intervention

Erick P. de Oliveira, Lidiana C. Talon, Fernando Moreto, Viviane M R Acerra, Katia C P McLellan, Roberto C. Burini. UNESP - Univ Estadual Paulista, Medical School, Botucatu, Brazil.

(No relationships reported)

Metabolic Syndrome (MetS) is a cluster of diseases associated with sedentary and food inadequacy. Lifestyle modification programs (LSMP) including physical activity and nutrition re-education are the aim of the primary care of MetS being the management of body adiposity and plasma lipids one of the goals. Dietary supplements of omega-3 polyunsaturated fatty acid (W-3 PUFA) have those properties.

PURPOSE: To compare the additional effect of dietary supplements of W-3 PUFA to a LSMP in treated MetS of free-living adults.

METHODS: In a prospective clinical trial, we studied 61 adults (50±13.8 years old), 85% of women, clinically screened to participate in a LSPM. The subjects were randomly assigned in two groups: LSMP (G1, n=26), including daily sessions (5×/wk) of supervised exercises of walking (3×/wk, 60-70% VO2max) and strength (2×/wk, 65-80% 1RM) along with a weekly dietary counseling. The other group (G2, n=35) also under LSMP received W-3 PUFA (3g/day). Anthropometric measurements (waist circumference, body mass index, body fat percentage), dietary intake (24-hour recall), cardiorrespiratory fitness (Balke protocol in treadmill, CRF), blood pressure (BP), and plasma biomarkers for MetS were performed at baseline (M0), and after 20 weeks (M1) of intervention. For statistical analysis it was used anova-two way, adjusted for gender, age, BMI and total caloric intake, and significancy when p<0.05.

RESULTS: After 20 weeks both groups increased the CRF, but only G2 showed significantly decrease in waist circumference (1.3%) followed by MetS reduction (29%) mainly due to the normalization of BP (33,3%) and triglycerides (27,3%).

CONCLUSIONS: The dietary-supplements of W-3 PUFA potentiated the MetS treatment effects of LSMP by its effects on abdominal fat and some blood markers. Supported by CNPq, CAPES and FUNDAP

1238 Board #19 May 30 9:30 AM - 11:00 AM

Effect Of Exercise Training On The Inflammation Status Among People With Metabolic Syndrome.

dorthe Stensvold, Ulrik Wisløff, Stig A. Slørdahl. Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, Trond, trondheim, Norway.

(No relationships reported)

Metabolic syndrome is associated with chronic low-grade inflammation, a condition thought to play a key role in the pathogenesis of the syndrome. Among a number of pro-inflammatory cytokines, interleukin 18 (IL 18) seem to be the best marker for inflammation among people with metabolic syndrome.

PURPOSE: The aim of the study was to examine the effect of aerobic training versus strength training on circulating IL 18 and other pro-inflammatory markers in people with metabolic syndrome.

METHODS: Thirty-one inactive men and women (49.8 ± 9.1 years) with metabolic syndrome were randomized to either high intensity interval training (AIT, n=11), strength training (ST, n=10), or to a control group (n=10). Exercise training was carried out 3 times per week for 12 weeks. Serum insulin, high sensitive C-reactive protein (hs-CRP), interleukin 18 (IL 18), interleukin 6 (IL 6), and tumor necrosis factor-alpha (TNF-α) were measured before and after the intervention.

RESULTS: Serum IL 18 was reduced by 43% after AIT (p<0.001). Although AIT had no change in TNF-αfrom baseline, the levels were lower compared to ST (p=0.032) and control group p= 0.039) after the intervention. Total body fat was reduced after AIT (from 33.9 ± 7.3% to 32.2 ±7.9%, p<0.001) and ST (from 31.2 ± 3.9% to 29.7 ± 3.4%, p=0.025). There were no changes in serum IL 6, insulin, or hs-CRP within or between the groups.

CONCLUSIONS: Both strength training and high intensity aerobic exercise reduces fat mass, however, only the latter intervention is associated with a more favourable inflammatory status among people with metabolic syndrome.

1239 Board #20 May 30 9:30 AM - 11:00 AM

Development of a Minimal Exercise Regimen With Maximal Benefits for Type 2 Diabetics

Kathleen Broomall1, Avi Milgrom1, Dan Carl2. 1 Miami University, Hamilton, OH. 2 University of Cincinnati, Cincinnati, OH.

(No relationships reported)

PURPOSE: To examine the effectiveness of a novel, minimal exercise regimen based upon brief, strategically timed bouts of moderate intensity in attenuating postprandial glucose spikes as well its effectiveness in weight management over hours, days and years for type 2 diabetics. The regimen requires no exercise clothing or equipment; thus it can be readily integrated into a busy day in a “built environment” –unlike other brief-interval regimens The underlying premise, documented in the literature, is that muscle contractions can shunt blood glucose from the blood stream into muscle using interval style muscle work.

METHODS: Blood glucose levels were mapped over two time scales: hours and years. The short-term studies were to preliminarily explore how the exercise regimen attenuates blood glucose spikes; thus metabolic cart data was gathered in addition to blood glucose. Specifically, the short term studies mapped blood glucose curves as well as fat and carbohydrate utilization from repeated 2 minute bursts of physical activity in three scenarios: no exercise before or after eating (AUC 6095), exercise after eating but with diminished exercise frequency before the meal (AUC 5680), and normal frequency before and after the meal (AUC 1962). The long-term studies tracked daily blood glucose levels and appropriately timed HbA1C’s over a 30-month period.

RESULTS: Short-term blood glucose attenuation was significantly more effective with the proposed exercise regimen using the third scenario. Additionally, carbohydrate utilization was significantly greater with the proposed regimen. Finally, glucose management yielded significantly improved A1C’s as a clinical measure of glycemic control. Inhibition of weight regain with subsequent additive weight loss also seemed a long term trend of this regimen.

CONCLUSIONS: An exercise regimen of repeated brief bouts of moderate intensity executed after caloric intake can be effective in managing blood glucose and long term weight management in type 2 diabetics. Proposed research will map HbA1c values against degrees of adherence to the regimen and will identify psychological and physical factors moderating adherence. The ultimate goal is to deliver the regimen to clinicians with guidelines for the selection of viable candidates.

1240 Board #21 May 30 9:30 AM - 11:00 AM

Glucose Tolerance: A Comparison Of Sitting vs. 0.5 Mph Walk On The Active Workstation

Ronald Cox, Christina Ohlinger. Miami University, Oxford, OH.

(No relationships reported)

A growing body of literature suggests that striking differences in metabolic responses to nutritional challenge can be demonstrated when the postural condition of the individual is changed from sitting to standing.

PURPOSE:To describe the possible differences in glucose clearance, in young healthy individuals, in response to a glucose tolerance test performed under standard sitting conditions or while using an Active Workstation at very low speeds.

METHODS: Nine students, eight females and one male (21±1 yrs) performed a series of glucose tolerance tests. After a minimum of an 8-hour fast a baseline glucose measurement (Accu-Chek®) was obtained. Subjects then ingested 75 gm of glucose solution (Trutol®75) within a ten-minute period. In a counterbalanced manner, subjects either spent the two hours sitting or walking at 0.5 mph on an Active Workstation. During the session, a blood sample (finger stick) was obtained every 30 min for the next two hours.

RESULTS: Table 1 shows the means ± SD for blood glucose for each time period under the two conditions. All subjects showed a lower blood glucose curve during walking. The levels at 90 and 120 min were significantly lower (p< 0.05) in the walking condition.

CONCLUSIONS:These results support the impact of even very low levels of physical activity on metabolic response to glucose ingestion compared to sitting in healthy people. In fact, walking at a speed of 0.5 mph is a near proxy for standing. This result has implications for the standardization of glucose tolerance tests and reaffirms the potentially deleterious effects of sitting while offering support for the benefits of movement in normalizing blood glucose.

Table

Table

1241 Board #22 May 30 9:30 AM - 11:00 AM

Dietary-fibre Adequacy Potentiates Physical Exercises In Metabolic Syndrome Management

Livia S. Gonçalves1, Marita S. Mecca1, Franz H P Burini2, Reinaldo C. Dalanesi1, Vanessa L. Peresi1, Roberto C. Burini1. 1 UNESP - Univ Estadual Paulista, Medical School, Botucatu, Brazil. 2 USP - PRONUT, São Paulo, Brazil.

(No relationships reported)

Lifestyle modification focusing on dietary and exercise behaviors is the preferred first-line treatment for the management of metabolic syndrome (MetS), its components and co morbidities. However attempts to modify diet by reducing food intake are often unsuccessful due to the low compliance. Adequate-to-high fibre intake appears as an alternative for limiting energy intake in a society with high energy-dense food consumption.

PURPOSE: To investigate the effects of fibre intake on exercised-MetS patients in a prospective dynamic cohort study.

METHODS: The subjects that fulfilled all the inclusion criteria totalized 253 (mostly 35-60 years old and females). They all accomplished a protocol of lifestyle modification program (LSMP) including supervised physical exercise of walking (3 X/wk: 60-70% maximal heart rate) and strength (2 X/wk: 65-80% of one repetition maximal) along with a weekly dietary counseling. A group of 131 subjects was studied on traditional LSMP during 24 weeks (G1), another group of 72 subjects were randomized in either fibre (G2A = 25-30 g fibre/day) and non fibre (G2B) adequacy groups in a 20 weeks-lasting intervention, and the remainders 50 patients also received similar treatment of fibre intervention (G3A) or only LSMP (G3B) but in a shorter (10 weeks) experiment. The statistical analysis (paired Student’s t test) were used to compare moments (p<0.05).

RESULTS: MetS varied from 40% to 50.4% (NCEP-ATP III, 2003 criteria) at the baseline and the recalled dietary fibre intake averaged 16,0 ± 7,1 g/day. There was a decreasing in MetS with the LSMP alone lasting either 24 wks (-24.2%) or 20 wks (-11.5%) but not after 10 wks (+6%). These decreasing was optimized by dietary fibre interventions from 24% (G3A: 10 wks) to 36.4% (G2A: 20 wks). At the end of the experiment the increase of fibre intake varied from 7.2 g/d (G1) to 14.6 g/d (G2A) and 16.9 g/d (G3A), above the baseline values. The reduced obesity rate varied from 7% to 9% in the fibre-intervened groups.

CONCLUSIONS: The present data support the use of fibre adequacy as reducing factor for energy-dense food consumption and consequently body weight loss and then accelerating MetS decrease in a complementary physical exercise protocol. Supported by CNPq and FUNDAP

1242 Board #23 May 30 9:30 AM - 11:00 AM

The Impact Of Breaking Sitting Time On Postprandial Response To Three Separate Meal Replacement Beverages.

Meredith C. Peddie, Nancy J. Rehrer, FACSM, Murray Skeaff, Tracy L. Perry. University of Otago, Dunedin, New Zealand.

(No relationships reported)

PURPOSE: The purpose of this study was to compare the effects of breaking continuous sitting with short regular exercise breaks or one continuous bout of exercise on postprandial serum glucose, insulin, and triglyceride responses to three meal replacement beverages.

METHODS: Seventy participants (Mean (SD) VO2max 42.9 (10.3) ml·kg·min-1, Body mass index 23.6 (4.0)) completed three 9 h interventions. During the prolonged sitting intervention (SIT), participants sat continuously for 9 h; in the exercise intervention (EX) participants sat for 15 min, walked on the treadmill for 30 min, then sat continuously for 8 h and 15 min; in the breaking sitting time intervention (BRK) participants regularly interrupted their sitting with eighteen 1 min 40 s bouts (total of 30 min) of exercise. In each intervention participants were fed a MRB at 60, 240 and 420 min. Each MRB contained 31.4 ± 6.6 g fat, 36.8 ± 7.7 g protein 76.4 ± 16.1 g CHO and 3105 ± 652 kJ energy. Blood samples were obtained at baseline and hourly for 9 h, with additional samples collected 30 and 45 min after each feeding. Postprandial area under the curve (AUC) responses were calculated in response to the three separate MRB. Comparisons between interventions were made using mixed model regression.

RESULTS: The triglyceride AUC in BRK was 8% (95% CI 0.5 to 16.0% p=0.03) greater compared to SIT in response to the 2nd MRB. There were no differences between conditions in response to the 1st or 3rd MRB. The glucose AUC in BRK was 5.8% (95% CI 2.5 to 9.1% p=0.04) less than SIT in response to the 1st MRB. These differences were maintained in response to the 2nd MRB, but where no longer apparent in response to the 3rd MRB. The insulin AUC in BRK was 11.2% (95% CI 1.8 to 20.7% p=0.01) less than SIT in response to the 1st MRB. In response to the 2nd MRB BRK was 41.9% (95% CI 15.4 to 68.3% p=0.02) less than SIT. The response to the 3rd MRB was similar to the 1st MRB.

CONCLUSIONS:Breaking sitting time reduces postprandial insulin responses throughout the day. Health promotion guidelines should promote regular physical activity breaks during periods of prolonged sitting, particular for those at risk of insulin resistance.

1243 Board #24 May 30 9:30 AM - 11:00 AM

Chronic Exercise Training Effects on Fatigue in Multiple Sclerosis: A Meta-Analysis

Tina A. Mattila, Megan Nickrent, Lara A. Pilutti, Robert W. Motl, Steven J. Petruzzello, FACSM. University of Illinois at Urbana-Champaign, Urbana, IL.

(No relationships reported)

Fatigue is a common, disabling, and poorly managed symptom of multiple sclerosis (MS). Chronic aerobic exercise training may be an effective behavioral strategy for reducing fatigue in persons with MS.

PURPOSE: Provide a quantitative review of research examining the effect of exercise training on fatigue in MS.

METHODS: Electronic databases (Web of Science, PubMed, PsycInfo, Medline, Google Scholar, and Current Contents Plus) were searched for articles published up to September 2011 by using the key words exercise, fatigue, tiredness, energy, mood, lassitude, physical activity, rehabilitation, and fitness in conjunction with MS. Manual search of bibliographies of the retrieved papers was also done and study authors were contacted about additional studies. Thirty-five journal articles were located and reviewed, and 25 provided enough data to compute effect sizes (ES) and include in the meta-analysis. The meta-analysis was conducted with Comprehensive Meta Analysis using a random effects model and the overall ES was expressed as Hedge’s g.

RESULTS: Twenty-five ESs were retrieved from the 25 studies with 606 MS participants and yielded a weighted mean ES of 0.55 (95% CI = 0.37, 0.78). The weighted mean ES was heterogeneous, Q = 50.71, df = 24, P = 0.001, supporting a search for moderator variables.

CONCLUSIONS: The cumulative evidence supports that exercise training is associated with a fairly sizable reduction in fatigue among persons with MS.

1244 Board #25 May 30 9:30 AM - 11:00 AM

The Effect Of Aerobic Exercise On The Quality Of Life Amongst HIV Positive Persons

Louis J. Holtzhausen, Sorita Viljoen, Marlene Schoeman. University of the Free State, Bloemfontein, South Africa. (Sponsor: Jon Patricios, FACSM)

(No relationships reported)

The positive effects of regular exercise on the physiological well-being of HIV positive (+) persons have been well documented. Although evidence exist for HIV+ persons to retain muscular strength and improving work-related performance through regular exercise without compromising their immune capacities, limited research has been done to assess the effect of exercise on non-physiological parameters such as pain, functional limitations and social functioning which influence overall quality of life (QoL). Aerobic exercise results in several health benefits and consequent QoL improvements in HIV negative populations. However, it is uncertain how aerobic exercise would influence the QoL (self-perceived physical and mental well-being) of HIV+ persons.

PURPOSE: To determine the effect of an aerobic exercise intervention on the QoL in HIV+ persons.

METHODS: Twenty eight HIV+ persons were randomised into two groups (aerobic intervention and control, n=14 each). The intervention group was exposed to a 12 week supervised aerobic intervention, while the control group was exposed to a supervised stretching program to limit drop-out without inducing physiological adaptation. Participants were assessed for their aerobic capacity, immunological status (CD4 and viral load) and QoL score before and after the intervention. Significance between pre- and post intervention results were set at p < 0.05.

RESULTS: Following the intervention, a significant improvement (p = 0.02) in aerobic capacity were seen for the intervention group, while their CD4 counts and viral loads remained unchanged. Interestingly, both groups displayed significant improvements in their self-perceived physical and mental well-being (p = 0.003 and p = 0.002 for the intervention and control group respectively). Based on research in other population groups, the improved QoL in the control group is speculatively attributed to a placebo effect achieved through the personal attention received during exercise supervision.

CONCLUSIONS: Aerobic exercise leads to an improved aerobic capacity without compromising the immune capacities of HIV+ persons. Supervised exercise seems to improve QoL for HIV+ persons, irrespective of the type and intensity of the exercise and should therefore be promoted in the holistic disease management of HIV+ persons.

1245 Board #26 May 30 9:30 AM - 11:00 AM

Effects of Self-Selected and Imposed Intensity Acute Exercise on Psychological and Cortisol Responses

Kyoko K. Wardwell, Brian C. Focht, FACSM, Courtney A. DeVries, Ann A. O’Connell, Janet Buckworth, FACSM. The Ohio State University, Columbus, OH.

(No relationships reported)

PURPOSE: The primary purpose was to examine psychological responses to self-selected and imposed-intensity acute exercise in inactive women with high levels of stress. The secondary purpose was to examine potential psychobiological mechanisms (HPA-axis response and self-efficacy) for changes in psychological states.

METHODS: Nineteen participants (age = 23.58 ± 5.30 yr) completed 3 trials of treadmill exercise at self-selected intensity, 10% above and 10% below relative self-selected intensity. Exercise duration was determined to expend 150 kcal. Affective responses and salivary cortisol were measured prior to, during, and following exercise. Self-efficacy was also measured during and post-exercise. Future exercise intention was measured post exercise.

RESULTS: Affect and self-efficacy improved significantly over time at all exercise intensity conditions (all variables were p < .005). Moreover, positive engagement was higher at self-selected than imposed intensity during and post exercise (p < .05). No significant associations were observed between affect, self-efficacy and cortisol levels. However, affect and self-efficacy did significantly predict future exercise intentions.

CONCLUSIONS: Acute bouts of exercise at intensities proximal to and lower than ventilatory threshold are associated with positive affective responses during and after exercise in women with high levels of stress. Self-selected intensity may be effective for eliciting more favorable experiences during and following acute bouts of exercise, and promote future intentions for exercise. Findings provide partial support for self-efficacy during exercise as a potential mechanism for positive affective responses, especially at self-selected intensity.

1246 Board #27 May 30 9:30 AM - 11:00 AM

Regular Exercise Attenuates Oxidative Stress in Aging Rat Liver : Apoptosis and Fibrosis Signal Pathway Toward Anti-Aging Medicine

Kai-Wen Chang1, Chi-Chang Huang1, Wan-Teng Lin2. 1 National Taiwan Sport University, Taoyuan County, Taiwan. 2 Tunghai University, Taichung, Taiwan.

(No relationships reported)

Regular exercise is known to exhibit various health benefits in reducing risks of age-related diseases such as cardiovascular diseases, type 2 diabetes and cancer. However, there is no report about the protective effects of exercise training on liver senescence.

PURPOSE: The purpose of this study was to investigate whether a 12-week swimming exercise training can suppress senescence-oxidative stress markers of liver tissues.

METHODS: Twenty-three male Sprague-Dawley rats were divided into the following four groups: (1) vehicle control (n = 6), (2) aging (n = 6), (3) swimming exercise (n = 5), and (4) aging with swimming exercise (n = 6). Rats in groups A and AS were intraperitoneal injection with D-galactose (150 mg/kg/day) for 12 weeks to induce aging. Rats in groups SE and ASE were subjected to swimming exercise for 60 min/day × 5 days/week for 12 weeks. Body weight, liver weight, epididymal fat mass, histopathological, TUNEL analysis and and expressions of Cytochrome C, Bax, Bcl-2, Caspase 9, cleaved Caspase 3, cleaved PARP, MMP2 and MMP9 in liver apoptosis and fibrosis pathway are detected.

RESULTS: The results show that apoptosis index cleaved Caspase 3 and cleaved PARP were significantly lower in C and ASE than in A group (P < 0.05) ; apoptosis protective protein Bcl-2 is significantly higher than A group (P< 0.05). In ASE group through increasing PI3K-Akt signaling activities and inhibiting apoptosis and fibrosis pathway activities compared with aging group. be a major mechanism of anti-aging.

CONCLUSIONS: These results suggest that a 12-week swimming exercise may suppress apoptosis and fibrosis markers in the liver tissues of D-galactose induced aging rats. This study was supported by a research grant from the National Science Council, Taiwan (NSC99-2410-H029-059-MY2)

1247 Board #28 May 30 9:30 AM - 11:00 AM

Exercise Training Counterbalances Chronotropic Incompetence and Delayed Heart Rate Recovery in Systemic Lupus Erythematosus

Fabiana Benatti, Renata Miossi, Ana Lúcia de Sá Pinto, Fernanda Lima, Eduardo Borba, Danilo Prado, Bruno Gualano, Eloísa Bonfá, Hamilton Roschel. University of São paulo, São Paulo, Brazil.

(No relationships reported)

PURPOSE: To evaluate the efficacy of a three-month exercise training program in counteracting the chronotropic incompetence and delayed heart rate recovery in patients with systemic lupus erythematosus.

METHODS: A 12-wk, randomized trial was conducted. Twenty four inactive SLE patients were randomly assigned into two groups: trained (T, n=14, three-month exercise program) and non-trained (NT, n=10). A gender-, BMI-, and age-matched healthy control group (C, n=8) also underwent the exercise program. Subjects were assessed at baseline (PRE) and 12 weeks after training (POST). Main measurements included the chronotropic reserve (CR) and the heart rate recovery (ΔHRR) as defined by the difference between HR at peak exercise and at both first (ΔHRR1) and second (ΔHRR2) minutes after the exercise test.

RESULTS: Neither the non-trained SLE (NT) patients nor the healthy control group (C) presented any change in the chronotropic reserve (NT PRE: 72.1±11.1, POST: 75.6±19.2 %, p=0.96; C PRE: 93.5±4.9, POST: 95.9±10.4 %; p=0.99) or in ΔHRR1 (NT PRE: 26.6±11.8, POST: 26.7±10.7, p=1.0; C PRE: 33.8±6.6, POST: 38.2±10.0, p=0.95) and ΔHRR2 (NT PRE: 38.8± 13.5, POST: 39.5±15.6, p=1.0; C PRE: 52.0±5.7, POST: 53.6±7.6; p=0.99). The exercise training program was effective in promoting significant increases in chronotropic reserve (T PRE: 79.4±13.5, POST: 95.4±9.6 %, p=0.003) (POST T vs. NT, p=0.0003; ES=1.15), and in ΔHRR1 (T PRE: 23.1±9.6, POST: 41.0±10.7, p=0.0006) (POST T vs. NT, p=0.02; ES=1.12) and ΔHRR2 (T PRE: 39.0±10.5, POST: 57.3±12.4, p=0.0007) (POST T vs. NT, p=0.01, ES=1.11) in the SLE trained group (T) when compared with the NT group. Moreover, the heart rate response in SLE patients after training achieved parameters comparable to the healthy control subjects, as evidenced by the ANOVA and by the Z-score analysis (p>0.05, T vs. C). SLEDAI scores remained stable throughout the study.

CONCLUSIONS: A 3-month exercise training program was safe and capable of reducing the chronotropic incompetence and the delayed heart rate recovery observed in physically inactive SLE patients.

Supported by Fapesp (11/08302-0)

1248 Board #29 May 30 9:30 AM - 11:00 AM

Parasympathetic Tone And Its Adaptation To Aerobic Training (Fitwalking®) In HIV Patients On Anti-Retroviral Therapy

Giampiero Merati1, Antonio La Torre1, Matteo Bonato1, Gaspare Pavei1, Simona Bossolasco2, Laura Galli2, Paola Cinque2. 1 University of Milan, Milan, Italy. 2 San Raffaele Scientific Institute, Milan, Italy.

(No relationships reported)

Recent evidences showed that in HIV patients Highly Active Antiretroviral Therapy (HAART) and HIV per se may affect parasympathetic tone; this may increase the cardiovascular risk and contribute to HIV associated lipodystrophy. Such impairment may be reversed by a regular physical activity, which is known to increase vagal tone. PURPOSE: To evaluate vagal tone and its change after 3 months of aerobic training in HIV patients on HAART.

METHODS: 12 non-hypertensive and non-smoker patients (F/M 2/10; 47±5 yrs; 71±14 kg) with HIV infection (H) on HAART were enrolled. A group of 12 control (C) subjects (F/M 3/9; 48±12 yrs; 76±13 kg), matched for gender, age and anthropometric features, was enrolled for baseline comparisons. Heart rate variability (HRV) indexes of parasympathetic tone in time (RMSSD, pNN50) and frequency (High Frequency [HF] in absolute and normalized units [nu]) domains, and a HRV index of sympathovagal balance (LF/HF ratio) were calculated in basal condition in H and C subjects, both in supine and standing position for 5 min each, by a HR monitor. The H group was then trained by walking at 60-70% of maximal HR 3 times/week for 12 weeks, and the HRV measures were repeated thereafter. A 6-min walking test (6MWT) was executed before and after the training.

RESULTS: In basal condition, despite similar values in supine position between H and C subjects, HIV patients showed a lower parasympathetic tone during standing, which was significant in the linear indexes (RMSSD: H 16.7±5.1 vs C 25.3±10.5 ms [p<0.05]; pNN50: H 1.4±1.3 vs C 5.4±5.3% [p<0.05]; HF: H 180±207 vs C 196±139 ms2; HFnu: H 21.6±23.5 vs C 27.0±16.2), and a higher sympathetic activation (LF/HF: H 9.2±7.0 vs C 4.1±2.7 [p=0.04]) compared to C subjects. Fitwalking® training significantly improved the distance covered by 6MWT (from 667±50 to 802±160 m, p=0.01) and the physical work done (from 451±96 to 538±132 J, p=0.01). However, standing values of HRV indexes did not significantly change after training in H patients (RMSSD 15.2±6.6 ms, pNN50 1.1±1.7%, HF 100±93 ms2, HFnu 11.5±6.7, LF/HF 9.7±7.5; p=ns vs pre-training for all comparisons).

CONCLUSIONS: 12-weeks aerobic training improved cardiovascular fitness in HIV patients, but did not affect the heart parasympathetic control, which remained reduced compared to healthy subjects.

1249 Board #30 May 30 9:30 AM - 11:00 AM

Oxidative Stress Biomarkers in HIV+ Sedentary Patients And Its Relation to HAART Administration Time

Alesandro Garcia1, Géssica A. Fraga1, Fabiano Dmytro L. Pinto1, Gabriela G. Martins1, Carolina Mendes S. Silva1, Roberto Carlos V. Junior1, Adilson R. Filho1, Marisa C. Voltarelli1, Michel B. Araújo2, Flávia Carolina L. Melo1, Fabricio A. Voltarelli1. 1 Federal University of Mato Mato Grosso (UFMT), Cuiabá-MT, Brazil. 2 Sao Paulo State University (UNESP), Rio Claro-SP, Brazil.

(No relationships reported)

PURPOSE: The goal of the present study was to analyze the antioxidant enzymes activities as well as a lipid peroxidation marker in the blood of HIV seropositive (HIV+) sedentary patients.

METHODS:This study assessed the effect of different Highly Active Antiretroviral Therapy (HAART) administration times on oxidative stress (OS) biomarkers of HIV+ sedentary patients. Twenty-two HIV+ subjects (Men n=11; 39.42±9.44 years old; Women n=11; 45.00±6.59 years old) were divided into 3 groups, according to the HAART administration time: from 0 to 5 years (0/5y; n=6); from 6 to 10 years (6/10y; n=7); and from 11 to 15 years (11/15y; n=9). The activity of catalase (CAT [U/mg Hbmin]), superoxide dismutase (SOD [U/mg Hbmin]) and glutathione peroxidase (GPx [U/mL]) enzymes as well as a lipid peroxidation marker (thiobarbituric acid reactant substances; TBARs [μmol/L]) were determined in the blood serum of patients by commercial kits (Cayman Chemical, Michigan, USA). The data were analyzed using Kruskal-Wallis test and Dunn post hoc test (p value of <0.05).

RESULTS: The mean values of SOD (2.67±0.41) and GPx (7.89±0.22) activities were lower in the 0/5y group when compared to 6/10y (SOD: 4.25±0.32; GPx: 9.57±0.66) and 11/15y (SOD: 5.91±0.34; GPx: 13.08±0.33) groups; if compared the 6/10y and 11/15y groups, the means values (SOD and GPx) were statistically different (11/15y > 6/10y). In relation to CAT activities and to TBARs concentrations, it were only observed significant differences when compared 0/5y (CAT: 2.39±0.36; TBARs: 4.14±0.35) and 11/15y (CAT: 4.37±0.24; TBARs: 6.08±0.44) groups. On the other hand, no differences were detected when these groups were compared to 5/6y group (CAT: 3.50±0.48; TBARs: 5.00±0.46).

CONCLUSIONS: The findings suggest that increased OS occurs additionally to persistent redox imbalance associated to HIV infection during apparently successfully HAART. In addition, the pro-oxidative responses seem to be related to HAART administration time. Finally, we believe that a physical exercise program would reverse, at least in part, this situation, once that regular physical exercise may protect the organism against the OS caused by the sedentarism condition.Supported by FAPEMAT (Brazilian Foundation; Process number: 512843/2009).

1250 Board #31 May 30 9:30 AM - 11:00 AM

Physical Training and Detraining and the Development of Nonalcoholic Hepatic Steatosis in Rats

Leandro P. Moura, Amanda Christine S. Sponton, Michel B. Araújo, Rodrigo A. Dalia, Marcelo C. Junior, Maria Alice R. Mello, FACSM. UNESP, São Paulo State University, Rio Claro, Brazil.

(No relationships reported)

Nonalcoholic Hepatic Steatosis (NAHS) is a disease caused by fat accumulation in the liver that can lead to organ failure and, consequently, to death. This disease has been increasing alarmingly worldwide and still little is known about the influence of physical exercise training and subsequent detraining on preventing NAHS.

PURPOSE: Analyzes body weight, insulin sensitivity, fat tissue accumulation and triglyceride (TG) concentration in the mesenteric adipose tissue and fat accumulation in the liver of rats submitted to physical training and subsequent detraining.

METHODS: Thirty weanling Wistar rats were utilized and divided into three groups: Control sedentary rats (C), Trained rats (T) submitted to physical exercise throughout the experiment, and Detrained rats (D) submitted to physical exercise for half of the experimental period and kept sedentary until the end of the study. The training protocol began when the rats were 28 days old and consisted of swimming 1hr per day, 5 days per week, at 80% of their individual anaerobic threshold (LAn), as previously determined by a lactate minimum test. The physical training lasted 16 weeks for the T group and 8 weeks for the D group.

RESULTS: An insulin sensitivity test was performed and 48h after this, the animals were sacrificed to analyze total weight and total amount of TG of the mesenteric adipose tissue and liver TG concentration. It was shown that physical exercise performed throughout the experiment increased the sensitivity of insulin (%removal of serum glucose/min) (C: 3.67 ± 0.61; T: 6.36 ± 3.19; D: 4.69 ± 1.20) and decreased the body weight gain (g) (C: 527.13 ± 71.39; T: 453.14 ± 57.37; D: 522.21 ± 78.15), serum insulin concentration (ng/ml) (C: 1.72 ± 0.46; T: 1.39 ± 0.34; D: 1.65 ± 0.49), mesenteric adipose total weight (g) (C: 4.56 ± 1.60; T: 3.40 ± 1.20; D: 5.27 ± 1.94) and total TG amount (mg/total tissue weight-1) (C: 54.26 ± 5.03; T: 41.03 ± 2.27; D: 59.66 ± 3.99) as well as the TG concentration in the liver (mg/100mg) (C: 12.31 ± 3.07; T: 7.82 ± 2.14; CS: 11.94 ± 4.27).

CONCLUSIONS: In summary, physical exercise at 80% of LAn, attenuates the disorders that trigger fat accumulation in the liver and, consequently, counteracts NAHS development. Physical exercise cessation neutralizes all the beneficial effects of the physical training.

Supported by: FAPESP (2010/12718-5).

1251 Board #32 May 30 9:30 AM - 11:00 AM

Efficacy Of A Multifaceted Intervention Program To Increase Physical Activity In Patients With Parkinson’S Disease; The Parkfit Trial

Marlies van Nimwegen1, Arlène D Speelman1, Sebastiaan Overeem1, Bart P. van de Warrenburg1, Katrijn Smulders1, Manon Dontje2, George F. Borm1, Frank J.G. Backx3, Bastiaan R. Bloem1, Marten Munneke1. 1 UMC St Radboud, Nijmegen, Netherlands. 2 Hanze University, Groningen, Netherlands. 3 University Medical Center Utrecht, Utrecht, Netherlands.

(No relationships reported)

PURPOSE: Many patients with Parkinson’s disease (PD) lead a sedentary lifestyle. Simply informing patients about the health benefits of physical activity is insufficient to change their sedentary lifestyle. We developed and evaluated a multifaceted behavioural program (ParkFit) aiming to increase the level of physical activity undertaken by patients with PD.

METHODS: 586 PD patients were randomly assigned to the ParkFit Program or an active control group (ParkSafe Program). The level of physical activity was measured at baseline and at 6 months using a standardized interview-based 7-day recall (LAPAQ, primary endpoint), an ambulatory activity monitor (secondary endpoint) and an activity diary (secondary endpoint). Results were analysed according to the intention to treat concept.

RESULTS: 562 patients (96%) completed both baseline and 6 months assessments. In the ParkFit group, patients increased their time spent to physical activities with 7% as assessed with the LAPAQ; patients in the control group became 1% less active. The difference between both groups was not statistically significant. When we specified the nature of the activities, patients in the ParkFit group increased their ‘outdoor and sports activities’ (+32%), while their time spent to household activities decreased (-14%). In the control group these differences were less than 4%.

CONCLUSIONS: This short term outcome of the ParkFit trial suggests that patients with PD can increase their outdoor activities with a specific multifaceted program. This increase seems to be accompanied by a decrease in time spent to household activities. At the congress we will present the results of the ParkFit trial after 24 months intervention and the potential health consequences of a change in lifestyle. (Funded by ZonMw (The Netherlands Organization for Health Research and Development (75020012)); The Michael J Fox Foundation for Parkinson’s research; VGZ; Glaxo Smith Kline; and National Parkinson Foundation; ClinicalTrials.gov number, NCT00748488.)

1252 Board #33 May 30 9:30 AM - 11:00 AM

Changes in Sedentary Time and Physical Activity of Livestrong® Cancer Survivor Program Participants

Sarah Hilgers-Greterman1, Nicklaus Redenius1, Jeremy Frost1, John Schuna, Jr.1, Riggs Klika, FACSM2, Gary Liguori1. 1 North Dakota State University, Fargo, ND. 2 Cancer Survivor Center, Aspen, CO.

(No relationships reported)

PURPOSE: The purpose of this investigation was to identify changes in sedentary time (SED) and physical activity (PA) of cancer survivors enrolled in a post-treatment, 12 week, Livestrong® group exercise program.

METHODS: Eleven cancer survivors (1 Male and 10 Females; 51.8±9.27 yrs, BMI=26.6±4.1), representing a variety of cancer and treatment backgrounds, volunteered and wore armband activity monitors (BodyMedia® SenseWear) for seven consecutive days. Data collection occurred during the first and sixth week (midpoint) of the Livestrong® Program, and each minute of activity monitor wear time was classified as sedentary (SED), moderate (MOD), or vigorous (VIG). Minutes from MOD and VIG were then combined into a moderate-vigorous (MV) category. Linear mixed model analyses were used to compare weekly time spent in SED, MOD, VIG, and MV between weeks 1 and 6 with an adjustment for activity monitor wear time (time-varying covariate).

RESULTS: Across both assessment periods, participants averaged > 15 hr·day-1 of non-sleep activity monitor wear time with a weekly total of (mean ± SD) 6673.6 ± 734.8 min·week-1 (week 1 = 6853.8 ± 572.9, week 2 = 6493.4 ± 856.8). No significant differences were observed in weekly minutes (mean ± SE) from week 1 to week 6 for SED (6484.5 ± 155.6 vs. 6114.6 ± 246.3, respectively), MOD (366.5 ± 67.7 vs. 373.8 ± 51.4, respectively), MV (369.3 ± 68.6 vs. 378.7 ± 51.5, respectively) or VIG (2.7 ± 1.3 vs. 4.9 ± 1.9, respectively).

CONCLUSIONS: Results indicated no significant difference in weekly time spent in SED or any PA category between week 1 and week 6. Time spent in both MOD and MV was well above the 150 minutes per week CDC recommendation of PA for adults without chronic conditions. However, VIG activity averaged less than 5 minutes per week. Due to the short time span and small sample, it is difficult to evaluate the overall efficacy of the Livestrong program. However, these preliminary results are promising in that MOD PA is well above the national recommended threshold and SED time appears to be decreasing, though not significantly.

1253 Board #34 May 30 9:30 AM - 11:00 AM

The Effects Of Exercise Training From Different Cancer Treatments In Cancer Survivors

City C. Hsieh1, Hsiao-Chuan Wen2, Chiuh-Chung Liu2, Chun-hong Lin2, Ling-Li Wang1. 1 Hsinchu University of Education, and Yuanpei University, Hsinchu, Taiwan. 2 Yuanpei University, Hsinchu, Taiwan.

(No relationships reported)

PURPOSE: The purpose of this study was to investigate the effects of exercise training on muscular fitness and quality of life in cancer survivors undergoing differing clinical treatments: surgery alone, chemotherapy following surgery, radiotherapy following surgery, or chemotherapy and radiotherapy following surgery.

METHODS: This study recruited four groups of female cancer survivors based on the type of clinical treatments they had received: surgery alone (S, n=10), surgery and chemotherapy (SC, n=10), surgery and radiotherapy (SR, n=10), surgery and chemotherapy and radiotherapy (SCR, n=10). The exercise sessions lasted for 60 minutes including a 10-minute warm-up, 40 minutes of aerobic exercise, resistance training, and 10 minutes cool-down, 3 days per week for 6 months. Exercise intensity ranged from 40% to 60% of heart rate reserve depending upon the participant’s health status. The assessments and reassessments of muscular fitness (handgrip, peak torque of shoulder, elbow, and knee flexion and extension using Biodex S4 Pro) and quality of life were performed before and after the 6-month exercise training.

RESULTS: Cancer survivors in 4 groups following the 6-month exercise intervention showed significant (p < .05) improvements in shoulder flexibility (+22.6∼38.0%) and extension (+26.6∼35.0%), elbow flexibility (+16.7∼43.0%) and extension (+20.7∼35.1%), and knee flexibility (+12.2∼27.5%) and extension (+8.6∼33.0%). In addition, handgrip was improved significantly (p < .05) in SC (+6.3%), SR (+9.8%), and SCR (+7.7%) groups, but not in the S group. Psychologically, all 4 groups showed significant (p < .05) improvements in total (+9.6∼18.8%), and in health (+13.8∼21.3%), social (+7.9∼20.5%), psychological (+9.3∼11.3%), and family (+6.9∼16.4%) quality of life. Moreover, no significant differences were observed between groups on any of the domains of muscular fitness and quality of life.

CONCLUSIONS: The current study suggested that moderate intensity exercise intervention is a safe and efficacious means to augment muscular fitness and improve the quality of life of cancer survivors. Moreover, the muscular fitness and quality of life of cancer survivors improved as a result of the exercise intervention in this study, regardless of the type of treatments.

1254 Board #35 May 30 9:30 AM - 11:00 AM

Randomized controlled trial Of Colorectal Cancer Exercise Program for Colorectal Cancer Patient After Surgery

Ki-yong An1, Mi kyung Lee1, Dong il Kim1, Dong hyun Kim1, Ji hee Min1, Nam-kyu Kim2, Justin Y. Jeon1. 1 Yonsei University, Seoul, Korea, Republic of. 2 Yonsei University Severance Hospital, Seoul, Korea, Republic of.

(No relationships reported)

Recently, incidence of colorectal cancer is remarkably increasing in Korea.

Exercise and physical activity have positive effects in colorectal cancer patients and they are revealed in many previous studies. Specially, the patients in recovery after surgery need exercise, so many surgeons recommend to walk for patients after surgery. However, except walking, there is not detail exercise manual. Also, among many previous studies, there is no exercise intervention study has colorectal cancer patients immediately after surgery for subjects.

PURPOSE: The purpose of this study are to examine the effect of colorectal cancer exercise program on recovery and physiological and psychological variables in colorectal cancer inpatient after surgery.

METHODS: Subjects in exercise group participated supervised exercise program 1∼2 times per day on colorectal patients undergoing laparoscopic surgery during hospitalization. Supervised exercise program is mainly composed of stretching, core exercise on the bed and walking. And subjects in conventional group used conventional protocol. Anthropometric measurements and fitness test assessed at pre-surgery, discharge from hospital.

RESULTS: The result showed that subjects in exercise group decreased hospital stay during hospitalization. Subjects between both group have no significant difference in anthropometric measurements and fitness level variation.

CONCLUSIONS: The finding demonstrated exercise program for colorectal cancer inpatients have a positive effect in recovery variables in colorectal cancer patients after surgery.

1255 Board #36 May 30 9:30 AM - 11:00 AM

Effect Of Exercise On Bone Mass Density In Women With Postmenopausal Osteoporosis: A Case-control Study

Marianna Broccatelli1, Antonio Sgadari2, Simona De Bellonia2, Roberto Bernabei2. 1 University of Study “Foro Italico”, Rome, Italy. 2 Catholic University of Sacred Heart, Rome, Italy.

(No relationships reported)

Osteoporosis is a common disease with a spectrum ranging from asymptomatic bone loss to disabling hip fracture. Most guidelines suggest regular weight-bearing exercise, and combined (aerobic and resistance) training to prevent and treat osteoporosis. However, a recent review concluded that physical exercise (PE) slightly improves bone density and does not reduce the risk of having a fracture in postmenopausal women.1 It has been hypothesized that this paucity of effect may be due to relatively short duration of interventions, poorly standardized exercise protocols and high rate of loss to follow up.

PURPOSE: To assess whether a combined exercise program (2 times/week callisthenic exercises plus 3 times/week aerobic and resistance training) influenced BMD in 155 women with postmenopausal osteoporosis.

METHODS: Forty-seven women with either lumbar or femoral osteoporosis, exercising at the University Hospital Fitness Facility for at least one year were identified as cases (EXE). One hundred and eight sedentary controls, out from a pool of 5,434 attending the Osteoporosis Outpatient Clinic, were selected according to the following matching criteria: female gender, age ± 1.5 years, duration of the observation period ± 3 months, initial BMD value ± 5% (SED). Main outcome was percent change in BMD. Comorbidities, medications, risk factors for osteoporosis, level of physical activity, dietary intake of macronutrients as well as of Ca, K, P were recorded.

RESULTS:Baseline characteristics were similar between EXE and SED groups: mean (±s.d.) age was 64.4±6.2 vs. 68.8±6.4 years (n.s.), BMD at femoral neck 0.581±0.067 vs. 0.598±0.063 g•cm-2 (n.s.), and BMD at lumbar spine 0.727±0.067 vs. 0.732±0.074 g•cm-2 (n.s.). After an average follow-up of 36.5±13.1 months, BMD was increased in the EXE group by 2.23% at the femoral neck and by 3.65% at the spine, while it was decreased in the SED group ( 2.59% and 0.65%, respectively) (p<0.01 at both sites).

CONCLUSIONS: Our results showed that sedentary postmenopausal women had a significant decline in BMD over a 3-year period, while women undergoing a combined PE program do increased their BMD both at the femoral neck and the lumbar level.

1. Howe TE et al. Exercise for preventing and treating osteoporosis in postmenopausal women. Cochrane Database Syst Rev. 2011 Jul 6;(7): CD000333

1256 Board #37 May 30 9:30 AM - 11:00 AM

Physical Exercise In Female Fibromialgia Patients: Effects On Pain, Functional Capacity And Symptomatology.

Ana Carbonell-Baeza1, Virginia A. Aparicio2, Inmaculada C. Alvarez-Gallardo2, Alejandro Romero2, Clelia M Martins-Pereira3, Ana M. Cuevas2, Pedro Femia2, Francisco B Ortega4, Pablo Tercedor2, Jonatan R. Ruiz4, Manuel Delgado-Fernández2. 1 University of Cadiz. Cadiz, Spain. University of Granada, Granada, Spain. 2 University of Granada, Granada, Spain. 3 Universidade Federal Da Paraíba, Joao Pessoa, Brazil. 4 University of Granada., Granada, Spain. Karolinska Institutet, Unit for Preventive Nutrition., Huddinge, Sweden.

(No relationships reported)

Exercise interventions have been extensively used for the management of fibromyalgia, but it is unclear which mode of exercise is more suitable for this population.

PURPOSE: To compare the effects of three different physical interventions (i.e. multidisciplinary, Biodanza and Taichi) on women with fibromyalgia.

METHODS: a total of 146 women (51.6±7.0 years) volunteered to participated in the study and were allocated in 4 groups: control (n=34), multidisciplinary(n=41), Biodanza (n=37) and Taichi (n=34). Multidisciplinary and Taichi interventions were carried out 3 times/week and Biodanza 1 time/week over three months. The following variables were assessed before and after the interventions: tender points, functional capacity (i.e. the handgrip strength, 6 minutes walk, 8 feet up & go, chair sit and reach and chair stand tests) and the fibromyalgia impact questionnaire (FIQ). One-way analysis of covariance (group as fixed factor, change score (posttest-pretest) as dependent factor and pretest score as covariate) was performed.

RESULTS: The Taichi group presented greater improvements on the 8 feet up & go test than the multidisciplinary (Change score: -2.1±0.2 vs. -1.1±0.2, p=0.005), the Biodanza (-2.1±0.2 vs. -1.1±0.2, p=0.006) and the control groups (-2.1±0.2 vs. -0.5±0.2, p<0.001). The multidisciplinary and Taichi groups showed greater improvements than the control group on chair sit and reach test (7.4 ±2.1 vs. -3.0 ± 2.2, p=0.005 and 9.9±2.3 vs. -3.0 ± 2.2, p=0.001 respectively). The FIQ total score improved in the multidisciplinary (-8.8±2.3 vs. 4.7±2.3, p<0.001), the Biodanza (-12.7 ±2.5 vs. 4.7±2.3, p=0.001) and the Taichi (-7.4±2.5 vs. 4.7±2.3, p=0.003) groups compared with the control group. The Biodanza group presented a greater decrease on number of tender points than the Taichi group (-1.4 ±0.4 vs. 0.1± 0.4, p=0.044).

CONCLUSIONS: The multidisciplinary, Biodanza and Taichi interventions improve the symptomatology in female patients. Taichi intervention improves the dynamic balance more than multidisciplinary and Biodanza interventions. Taichi and multidisciplinary interventions improve the lower body flexibility.

Supported by Ministry of Science and Innovation (BES-2009-013442 RYC-2010-05957), Instituto Andaluz del Deporte, CICODE (University of Granada) and MAPFRE Foundation.

1257 Board #38 May 30 9:30 AM - 11:00 AM

Heavy, Explosive Strength Training For Postmenopausal Women With Osteoporosis: A Pilot Study

Mats P. Mosti, Nils Kaehler, Astrid K. Stunes, Jan Hoff, Unni Syversen. Norwegian University of Science and Technology, Trondheim, Norway.

(No relationships reported)

Current exercise guidelines recommend weight-bearing activities and strength training for patients with osteoporosis. It is still unclear what type of strength training that is most beneficial for improving physical capacity and bone mass in these patients. PURPOSE: The present study investigated the effects of a heavy, explosive strength training programme on physical capacity, bone mineral density (BMD) and content (BMC) in postmenopausal women diagnosed with osteoporosis or osteopenia.

METHODS:Twentyone women with osteoporosis or osteopenia (t-score < -1.5 in hip or spine) were randomized to a training group (TG, n=10) or control group (CG, n=11). The TG underwent 12 weeks of supervised strength training in a squat machine, at an intensity of approximately five repetitions maximum, with emphasis on explosive initiation of the concentric part of movement. The CG was encouraged to do non-supervised training in line with current exercise guidelines. Measurements included one repetition maximum (1RM), rate of force development (RFD), serum markers of bone formation (procollagen type 1 N-terminal propeptide (P1NP)) and bone resorption (fragments of type I collagen C (CTX)), BMD, BMC and bone area measured by Dual X-ray absorptiometry. Nonparametric statistics were used. RESULTS: One participant was excluded in the TG and four participants withdrew (one in the TG and three in the CG). 1RM and RFD were significantly improved in the TG with 154±75% and 52±46%, respectively (p<0.05). BMC at the lumbar spine and femoral neck increased with 2.9±2.8% and 4.9±5.6%, respectively (p<0.05), while bone area increased with 2.4±2.0% and 5.2±5.1%, respectively (p<0.05). The 1RM and lumbar spine BMC improvements differed significantly from the CG (p<0.05). There was also a tendency to improved ratio of P1NP/CTX (21%, p=0.09) in the TG. No significant changes were observed in the CG.

CONCLUSIONS: Heavy, explosive strength training appears to be safe and give beneficial effects on muscle strength, RFD and BMC in postmenopausal women with osteoporosis. These findings should be further investigated in a larger trial of longer duration.

1258 Board #39 May 30 9:30 AM - 11:00 AM

Effective Physical Activity Energy Expenditure Feature For Maintaining Bone Mass In Postmenopausal Women

Zheng Lu, Zhi-xiong Zhou, Xiao-hong Chen, Xun Li, Yong-Sheng Wang. Capital University of Physical Education and Sports, Beijing, China.

(No relationships reported)

Growing evidence indicates that physical exercise can prevent or improve bone loss. However, the standard of the deficient exercise is lacking.

PURPOSE: To observe the change of energy expenditure (EE), body composition (BC), BMD and muscle strength before and after 1y exercise in postmenopausal women, to explore the relationship between the indexes and bone mass, and to determine the lest EE maintaining bone mass.

METHODS: 62 compliant women, aged 50-60ys, with BMD T-score <-1, were divided into exercise group (EG, n=32) and control (CG, n=30). EG exercised 3-4 times weekly for 1y. PA and EE were determined by accelerometer. BMD and BC were measured by DXA. Hip’s muscle strength was described by isokinetic peak-torque. Exercise intensity was assessed by portable metabolic test system. Paired and independent sample T tests were used within and between groups.

RESULTS: 1. EG were higher than CG in walking, physical activity(PA) and total diary energy expenditure (TDEE) (all p<0.01). And difference was mainly caused by walking EE, which should be considered effective PAEE.

2. PA time of 3-6MET in EG was more than CG (P<0.01). During the exercise, mean heart rate was 117.59±9.41bpm, maximum intensity 5.70±1.97MET, exercise time 50min, and EE 312.07±94.2Kcal. Moderate intensity exercise is adequate stimulus for maintaining EG bone mass.

3. After 1y, EG decreased in weight, BMI and F% (all p<0.01), and increased in LBM% (p<0.01). For both groups, weight, BMI, F%, muscle mass and TDEE was correlated with whole body BMD (r=0.486, 0.475, 0.434, 0.386, 0.366, all P<0.01), and LBM% was negatively associated with BMD (r=-0.481, P<0.01). The BC optimization was important feature.

4. After 1y, peak-torque values for hip flexion (90°/s, 180°/s) and extension (180°/s) in EG observably elevated (all P<0.01). BMD at main sites in EG were higher than CG (p<0.01-0.05). For EG, 1y exercise effectively increased muscle strength, which was the primary cause of maintaining bone mass.

CONCLUSIONS: 1. Exercise can effectively maintain bone mass by improving muscle mass and strength.

2. Exercise of 3-4 times weekly, 50 min/time, and 3-6MET’s moderate intensity is suitable for postmenopausal women to maintain bone mass.

3. Lower than 680 kcal PAEE can be considered as standard of deficient exercise for postmenopausal women maintaining bone mass.

1259 Board #40 May 30 9:30 AM - 11:00 AM

Effects Of Exercise On Physical, Psychological, And Social Aspects Of Pain In Spinal Cord Injuries

Carrie J. Yale, Petra B. Schuler, Ludmila Cosio Lima. University of West Florida, Pensacola, FL.

(No relationships reported)

Individuals who suffer a spinal cord injury (SCI) experience pain as a secondary result of the injury. Pain can be difficult to treat as the experience is unique to each individual. If not treated effectively, pain can elevate levels of depression, anxiety, stress, and social isolation.

PURPOSE: To determine if exercise helps alleviate aspects of pain associated with SCI.

METHODS: Six subjects participated in 8 weeks of twice weekly exercise. Participants were required to complete 12 minutes of wheelchair propulsion and 50 minutes of resistance training. Resting heart rate (HR), blood pressure (BP), cardiorespiratory fitness, muscular strength, pain, depression, anxiety, stress, and perceived social support were assessed. Descriptive statistics were computed to determine changes in resting HR and BP. Subjects performed 6 minutes of arm ergometry and cardiorespiratory fitness was measured by changes in the rate of perceived exertion (RPE) and HR for the same workload. RPE and HR were taken each minute; a dependent t-test was used to determine changes in these variables. During the 12 minute bout, HR, number of completed laps, and the RPE given for each lap around the track were reported; repeated measures of analysis of variance was used to compare any change in these variables. Muscular strength was determined by the subject’s one repetition maximum (1-RM). Self report questionnaires were used to measure pain, depression, anxiety, stress, and perceived social support. A dependent t-test was used to determine changes in the measures of the 1-RM and questionnaire scores. For all statistical analyses the confidence interval was set at p<0.05.

RESULTS: The group experienced improvement in measures of cardiorespiratory fitness as seen in a decreased resting HR (p = .043,), lower RPE at the fifth and sixth minute of arm ergometry (p = .042), lower RPE between each minute of arm ergometry (p = .029), and a lower RPE observed during the 12 minutes of wheelchair propulsion (p = .010). An increase in muscular strength for the chest press (p = .047) and deltoid press (p = .03) was observed. No significant differences were observed in the measures of pain, depression, anxiety, stress, and perceived social support.

CONCLUSIONS: Participation in weekly exercise improves physical functioning among individuals with SCI.

1260 Board #41 May 30 9:30 AM - 11:00 AM

The Effect of Low Back Strengthening on Balance Performance in Patients with Lumbar Sacralization

Yen-Ling Li1, Guo-Sheng Li1, Yu-Tsai Tu2, Wei-Han Chang3, Cheng-Hsiu Lai1, Shih-Wei Chou3. 1 Taipei Physical Education College, Taipei, Taiwan. 2 Chang Gung Memorial Hospital, Taipei, Taiwan. 3 Chang Gung Memorial Hospital, taipei, Taiwan. (Sponsor: Chia-Hua Kuo, FACSM)

(No relationships reported)

Lumbosacral transitional vertebre (LSTV), commonly found between the fifth lumbar vertebra and the first sacral vertebra, of which the transverse processes fuse to different extent. The changes in spinal dynamics of LSTV may result in early disc degeneration, turnk instability and consequent low back pain (LBP). Patients with chronic LBP and lumbar degenerative disease are also characterized by an anterior sagittal imbalance. However, it is unclear whether low back strengthening exercise improve balance performance in LBP patients with LSTV.

PURPOSE: To investigate the low back strengthening effects in LBP patients with LSTV on balance performance.

METHODS: Thirty LBP patients with LSTV were recruited and divided into two group randomizely assigned as Group A, the extensor-flexor group (age, 26.4 ± 11.2 years; body height, 171.9 ± 11.0 cm; body weight, 66.1 ± 13.5 kg) and group B, the flexor-extensor group (age, 26.1 ± 10.2 years; body height, 170.6 ± 8.4 cm; body weight, 66.0 ± 16.7 kg). Two session of the training program were prescribed including the extension session and the flexion session each for 3 weeks, and the order of the two training program was reversed between the two group. The results of the sensory organization test (SOT), Limits of St ability (LOS), and rhythmic weight shifting (RWS), measured by using the Smart Balance Master, lumbar ROM, measured by the microFET3, lumbar isometric strength, measured by the Cybex were compared between the two groups. LBP was evaluate by the visual analogus scale (VAS).

RESULTS: Irrespective to training group or modes , ROM , strength and VAS of low back were all improved significantly. Balance performance was in part improved significantly. Extensor training program seemed to be more effective than flexor traing program in pain relief.

CONCLUSIONS: Low back strengthening for LBP patients with LSTV may improve balance performance in part. It also improves isometric strength, ROM and pain scale of low back. The extensor training session may outweight flexor traing session in pain relief for patients with LSTV.

1261 Board #42 May 30 9:30 AM - 11:00 AM

Low Back Strengthening effects on Quadriceps Strength in Lumbago Patient with Lumbar Sacralization

Yi-chen Lin1, Chi-Ping Lai1, Yu-Tsai Tu2, Yin-Chou Lin2, Cheng-Hsiu Lai1, Shih-Wei Chou2. 1 Taipei Physical Education College, Taipei, Taiwan. 2 Chang Gung Memorial Hospital, Taipei, Taiwan. (Sponsor: Chia-Hua Kuo, FACSM)

(No relationships reported)

Lumbosacral transitional vertebre (LSTV), commonly found between the fifth lumbar vertebra and the first sacral vertebra, of which the transverse processes fuse to different extent. The changes in spinal dynamics of LSTV may result in early disc degeneration, trunk instability, and consequent low back pain (LBP). The patients with chronic LBP tend to show increased inhibition of quadriceps activity. However, it is unclear whether low back strengthening exercise improve the quadriceps strength in LBP patients with LSTV.

PURPOSE: To investigate the low back strengthening effects of the LBP patients with LSTV on quadriceps isokinetic strength.

METHODS: Thirty LBP patients with LSTV were recruited and divided into two groups randomizely assigned as Group A, the extensor-flexor group (age, 26.4 ± 11.2 years; body height, 171.9 ± 11.0 cm; body weight, 66.1 ± 13.5 kg) and group B, the flexor-extensor group (age, 26.1 ± 10.2 years; body height, 170.6 ± 8.4 cm; body weight, 66.0 ± 16.7 kg). Two sessions of the training program were prescribed including the extension session and the flexion session each for 3 weeks, and the order of the two training program was reversed between the two group. The isokinetic strength of the knee extensor, measured by the Cybex, were compared between the two groups. Pain of low back and knee were evaluate by the visual analogus scale (VAS).

RESULTS: Quadriceps strengths were significantly improved in all 3 angular velocities of both right and left legs, particularly after extensor training. Low back extensor training was more efficient than flexor in pain control of the knee pain.

CONCLUSIONS: Low back strengthening training for LBP patients with lumbar sacralization may improve quadriceps isokinetic strength and low back/knee pain scale, particularly after extensor strengthening.

1262 Board #43 May 30 9:30 AM - 11:00 AM

Low Normalized Leg Strength in Obesity Predicts Habitual Physical Activity and Endurance in Knee Osteoarthritis

Kevin R. Vincent, Cindy Montero, Amanda Seay, Heather K. Vincent. University of Florida, Gainesville, FL.

(No relationships reported)

Adults with knee osteoarthritis (OA) face multiple challenges with beginning exercise or participating in regular daily physical activity, especially if they are obese. It is unclear how normalized muscle strength relates to endurance time or habitual physical activity.

PURPOSE: To determine the associations between muscle strength, endurance time and habitual physical activity in obese and non-obese persons with knee OA.

METHODS: Participants with knee OA (68±7 yrs) who sought participation in an exercise program were stratified into obese (n=20; body mass index [BMI] 34±4 kg/m2, 49±9% body fat) and non-obese (n=20; BMI 25±3 kg/m2, 34±10% body fat). Maximal strength testing of the knee extensors and flexors, and maximal walking endurance tests were performed. Body fat was determined using the BODPOD. The Western Ontario McMaster Osteoarthritis (WOMAC) Index survey was administered to assess knee function with OA pain. Activity monitors were worn for 7 days to record daily activity. Regression analysis determined the contributions of normalized leg strength scores on treadmill endurance time, and average daily steps.

RESULTS: Walking endurance time was 31% lower in the obese group (p<0.0001). Average WOMAC knee pain was similar between groups (5.2-5.5 points). Average daily steps taken were 3246±1131 and 4248±1365 steps, in the obese and non-obese groups, respectively (p<0.005). The time spent performing moderate daily activity was 30 minutes less in the obese group (p=0.05). WOMAC scores were not different between groups (33.2 vs 28.8 points). Maximal normalized leg press, leg curl and leg extension strength values were less in the obese group (p<0.05). After accounting for age and sex, normalized leg strength values were contributors to the variance of the models predicting walking endurance time (model R2=0.514-0.561, R2 change range 0.218-0.265) and in habitual physical activity (R2=0.198-0.230, change range 0.104-0.136; all p<.001).

CONCLUSIONS: Walking endurance and habitual physical activity were consistently predicted by leg strength in knee OA. Targeted resistance exercise may improve muscle strength and foster improvements in endurance and increased habitual physical activity in obese individuals, both of which would facilitate weight loss. Supported by NIH NIAMS RO3AR059786

1263 Board #44 May 30 9:30 AM - 11:00 AM

Efficacy And Effectiveness Of Exercise On Tender Points In Adults With Fibromyalgia: A Meta-analysis

George A. Kelley, FACSM, Kristi S. Kelley. West Virginia University, Morgantown, WV.

(No relationships reported)

PURPOSE: Fibromyalgia is a major public health problem affecting an estimated 200 to 400 million people worldwide. The purpose of this study was to determine the efficacy and effectiveness of randomized controlled exercise intervention trials (aerobic, strength training or both) on tender points (TP) in adults with fibromyalgia.

METHODS: Meta-analysis using random effects models and 95% confidence intervals (CI) with results partitioned according to per-protocol and intention-to-treat analyses.

RESULTS: Statistically significant reductions in TP were observed based on per-protocol analyses (8 studies representing 322 participants) but not intention-to-treat analyses (5 studies representing 338 participants) (per-protocol, g, -0.68, 95% CI, -1.16, -0.20; intention-to-treat, g, -0.24, 95% CI, -0.62, 0.15). Changes were equivalent to relative reductions of 10.9% and 6.9%, respectively, for per-protocol and intention-to-treat analyses.

CONCLUSIONS: Exercise is efficacious for reducing TP in women with FM. However, a need exists for additional well-designed and reported studies on this topic.

1264 Board #45 May 30 9:30 AM - 11:00 AM

Case review for 12 Weeks Of High-intensity Interval Training (HIT) in a Spinal Cord Injured Man

Christopher R. Harnish, Jonathan A. Daniels, Allison F. Keeley, David R. Gater. Hunter Holmes McGuire VAMC, Richmond, VA. (Sponsor: Edmond Acevedo, FACSM)

(No relationships reported)

Exercise may play an important role in managing the metabolic health consequences of SCI but little is known about the impact of arm crank ergometer (ACE) HIT or how to adapt it for individuals with SCI.

PURPOSE: To review the outcome of 12 weeks of periodized HIT in a man with chronic traumatic SCI.

METHODS: One 42 yo man (180 cm tall, 68.4 kg and 30.6% Fat) with C8/T1 AIS A SCI took part in 12 weeks of 3 days.week-1 ACE interval training. A continuous 2 min stage VO2Peak test was completed before, 6 and 13 weeks after training. During the first training session and at week 4, 8 and 12 a 30 min time trial (TT30) test was performed on a Monark 881E ACE to assess performance improvement; heart rate (HR) and mean power (W) were determined. Training was conducted on a LODE ACE and consisted of a combination of HIT that included three times 5 min at ∼70% Peak Power (WPeak) and 5 min recovery (HIT5); four times 2.5 min at ∼85% WPeak and 5 min recovery (HIT2.5); ten times 1 min at ∼110% WPeak and 2 min recovery (HIT1). HR zones were set as <75% HRPeak (Z1), 75-89% (Z2), and 90+% (Z3) and HR was measured and stored for all training. HR training impulse (TRIMP) scores were calculated as: Z1 minutes + (Z2 minutes * 2) + (Z3 minutes * 3) and used to monitor overall training efficacy. DXA was used to estimate body fat pre and post training.

RESULTS: 35 sessions that included 8 HIT5, 10 HIT2.5, and 5 HIT1 sessions were completed. WPeak and VO2Peak improved about 45% and 52%, respectively, by week 6 without further improvement at week 12. TT30 wattage improved 32% at week 4, 47% at week 8, and 56% at week 12. HR TRIMP scores decreased in the first 6 weeks as mean session wattage increased before showing a parallel increase for the remaining 6 weeks.

CONCLUSIONS: Persons with SCI exhibit widely varying exercise responses, regardless of level of injury. This case report demonstrates that even high-level paraplegics can achieve exceptional training outcomes and that ACE HIT may provide an effective training method for SCI. As with able-bodied persons, maximal performance plateau early, while submaximal performance may show longer term improvement. The latter supports the use of submaximal training assessment important in the long-term training monitoring for SCI. HR TRIMP data provides a useful means for monitoring and periodizing training in paraplegia with a normal HR response.

1265 Board #46 May 30 9:30 AM - 11:00 AM

Comparative Study Of The Effects Of Tai Chi And Strength Training On Osteoarthritis In Older Adults

Abhinandan Batra, Yong Tai Wang, FACSM, Elisabeth Burgess, Ann Pearman. Georgia State University, Atlanta, GA.

(No relationships reported)

PURPOSE: Osteoarthritis (OA) is a degenerative joint disorder and one of the leading causes of disability in elderly. Tai Chi is seen to be effective in relieving symptoms of OA knee joint. The main aim of this study was to design a Tai Chi program and/or a strength training program for the older adults with knee OA and to compare the effects of these programs on the range of motion, gait, pain and balance.

METHODS: Twenty participants age 55 and over were divided into two groups (Tai Chi and Strength training) based on predetermined criteria (Age, Grade of OA, balance). Both groups received respective form of exercises twice a week for 2 months. Both groups were assessed before and after intervention on WOMAC, Berg balance scale, ROM and Manual muscle testing Independent and paired t test were employed to determine the differences within each group and between two groups with significance level at p<.05.

RESULTS: Mean ages of the participants in both strength training and Tai Chi group were 82.8± 10.6 years and 82±11.35 years respectively. Both the groups showed statistical significant improvement in WOMAC scores and balance. WOMAC score decreased for strength training group (52 to 40.5) and Tai Chi group (52.28 to 38.57). Balance score on the Berg balance scale improved for strength training group (36.75 to 46 at p<.05) and Tai Chi group (39.14 to 47.42 at p<.001). Significant increase in strength of lower limb muscle was seen in strength training group with no change in Tai Chi group. Though significant changes were seen within the group analysis, no such difference was found on comparing strength group to Tai Chi group.

CONCLUSIONS: Both groups demonstrated improvement in symptoms related to OA showing that both forms of exercise are effective and safe in improving symptoms related to OA but no evidence was found depicting Tai Chi to be better form of exercise than strength training.

1266 Board #47 May 30 9:30 AM - 11:00 AM

Physical Activity, Sedentary Time, And Bone Health In Younger And Older Females

Saori Ishikawa, Youngdeok Kim, Minsoo Kang, FACSM, Don Morgan, FACSM. Middle Tennessee State University, Murfreesboro, TN.

(No relationships reported)

Recent studies have focused on the contribution of physical activity (PA) and sedentary behavior (SB) on metabolism and cardiovascular health in adults. Little is known, however, regarding the extent to which PA and SB impact bone mineral content (BMC) and bone mineral density (BMD) in females across the lifespan.

PURPOSE: To examine the impact of PA and SB on bone health in females from adolescence to old age.

METHODS: Data from 2,245 females aged 12 years and older collected as part of the 2007-2008 National Health and Nutrition Examination Survey (NHANES) were included in this analysis. Based on self-report, participants were categorized into three PA categories: sufficient moderate-to-vigorous recreational physical activity (S-MVRPA), insufficient MVRPA (I-MVRPA), and no MVRPA (N-MVPRA). S-MVRPA was defined as ≥150 minutes per week of moderate recreational PA (RPA) or an equivalent amount of vigorous RPA for adults (≥19 years of age) or ≥60 minutes per day of MVRPA for adolescents (12 to 18 years of age). Values of BMC (g) and BMD (g/cm2) of total femur and total spine were measured by dual energy x-ray absorptiometry. Categories of PA and self-reported minutes of SB (i.e., sitting or reclining time, excluding sleeping) were used to predict BMC and BMD of the femoral and spinal regions in four age groups [i.e., adolescents (12-18 years), young adults (19-39 years), middle-aged adults (40-64 years), and older adults (>64 years)] after controlling for nutritional intake and body mass index (BMI). SAS 9.2 SURVEYREG procedures with ESTIMATE statement were used to account for the multistage and complex nature of the NHANES sampling scheme.

RESULTS: Female adolescents in the S-MVRPA category displayed significantly greater femoral BMC, femoral BMD, spinal BMC, and spinal BMD compared to those in N-MVRPA category (b=3.01, 0.07, 4.72, and 0.04, respectively, p<.05). In older adults, SB was also a significant predictor of femoral BMC and BMD (b=-0.004 and -0.0001, respectively, p<.05), such that an increase in SB resulted in lower femoral BMC and BMD.

CONCLUSIONS: These findings suggest that engaging in S-MVRPA during adolescence and reducing sedentary time in the elderly may contribute to improved bone health in females.

1267 Board #48 May 30 9:30 AM - 11:00 AM

Pilates is More Effective than Common Exercise Program for Treatment of Forward Head Posture

Sun-Myong Lee, Chang-Hyung Lee, Jung Jun Park. Pusan National University, Busan, Korea, Republic of.

(No relationships reported)

Background: Forward head posture (FHP) is defined as the anterior positioning of the cervical spine, which is one of the most common postural problems in modern society. Recently, Pilates has been reported to be effective exercise for correcting postural problems.

PURPOSE: To compare the effects of Pilates and common exercise program on improvements in craniovertebral angle (CVA), cervical range of motion (cROM), pain, and muscle fatigue in FHP

METHODS: Twenty eight sedentary women with FHP, aged between 20 and 39, participated in this study. They were randomly divided into two groups; Pilates exercise group (PG, n=14) and common exercise group (CG, n=14). Pilates exercise program consisted of modern Pilates focused on FHP-related major muscle group. Common exercise program consisted of stretching and resistance exercise on the same muscle group as Pilates exercise program. Both group performed exercise 50 min/day, 3 days/week, intensity of 11-15 RPE for 10 weeks. CVA, indicating FHP degree, was measured by X-ray. cROM, representing cervical function, was measured by CROM device. Pain levels were assessed by visual analog scale (VAS) and neck disability index (NDI). Muscle fatigue was measured by surface electromyogram (sEMG) at median frequency. Statistical significance was p< .05.

RESULTS: PG significantly increased CVA (65.65±4.53° vs. 70.09±4.78°, p=.002), but CG did not. PG also significantly increased 5 of 6 cROM items: flexion (35.11±6.39° vs. 46.64±8.83°, p=.004), extension (50.14±11.76° vs. 57.05±9.3°, p=.037), R. sideflexion (29.58±3.12° vs. 34.00±3.78°, p=.006), R. rotation (51.5±10.63° vs. 57.81±5.81°, p=.045), L. rotation (52.06±10.1° vs 57.17±6.65°, p=.033). However, CG did not change in CVA and cROM items. Among sEMG factors, only SCM was significantly increased in PG (27.27±11.26 Hz vs. 43.51±17.29 Hz, p=.009), but not in CG. VAS and NDI were significantly decreased in both groups.

CONCLUSIONS: These results indicate that Pilates is more effective to improve CVA, cROM, and, in part, muscle fatigue than common exercise (a combination of stretching and resistance exercise). It suggests that Pilates can be recommended as an appropriate exercise for FHP treatment, and may be extended for other patients with neuromusculoskeletal problems.

1268 Board #49 May 30 9:30 AM - 11:00 AM

Low Trunk Strengthening Effects on Patellofemoral Pain Syndrome

Chiping Lai1, Guosheng Li1, Chingya Hung1, Joupin Li1, Chenghsiu Lai1, Shihwei Chou2. 1 Taipei Physical Education College, Taipei, Taiwan. 2 Chang Gung Memorial Hospital, Taoyuan, Taiwan. (Sponsor: Chia-Hua Kuo, FACSM)

(No relationships reported)

Patellofemoral pain syndrome (PFPS) is a common knee problem. Quadriceps strengthening has been suggested to treat patients with PFPS. The quadriceps muscle was inhibited after lumbar paraspinal fatiguing exercise in the absence of quadriceps fatigue.

PURPOSE: To investigate low back strengthening effects on the patients with PFPS in terms of (1) low trunk isometric strength, (2) quadriceps isokinetic strength, and (3) knee pain scale.

METHODS: Eighteen knees of sixteen patients with PFPS were randomly divided into two groups, group A (extensor-flexor, 9 knees of 8 patients) and group B (flexor-extensor, 9 knees of 8 patients). In the 1st 3-week session of training group A started low trunk extensors strengthening and group B did flexor and in the 2nd 3-week session, the training programs were shifted between groups. The visual analog scale (VAS) was used to scale knee pain. Cybex NORM was used to measure isometric strength of low back flexor, extensor and isokinetic strength of quadriceps at 60°/sec, 120°/sec and 180°/sec. Two-way ANOVA with repeated measures in the 1st session tested the significance of main and interaction effects between training group factor and pre-post training factor. The training effects in the 2nd session were analyzed by prescription.

RESULTS: After the 1st session of training, interaction effects were significant (p < .05), indicating extensor training significantly increase low trunk extensor strength and vice versa. No interaction effects occur in isokinetic strength of the quadriceps between two groups at all three angular velocities. The VAS of knee pain appeared to be improved more in group A than B (51.83% vs. 36.07%). After the 2nd session, group B increased low trunk extensor strength and group A increased flexor strength. Isokinetic strength of the quadriceps in group B was improved more than group A at all three angular velocities. Extent of knee pain relief in group B seemed to be more than group A.

CONCLUSIONS:Low trunk strengthening training increases specific strength of either extensor or flexor muscles. However, extensor strengthening seemed to be more effective in patellofemoral pain relief and quadriceps strength enhancement.

A-30 Free Communication/Poster - Children and Exercise I

MAY 30, 2012 7:30 AM - 12:30 PM

ROOM: Exhibit Hall

1269 Board #50 MAY 30 11:00 AM - 12:30 PM

Odds Ratios and Sex Differences in Staying in at-Risk Fitness Categories among Middle School Children

Traci D. Zillifro, Wenhao Liu, Randall A. Nichols. Slippery Rock University, Slippery Rock, PA.

(No relationships reported)

Fitness tracking statistics such as Pearson or Spearman rank correlations, kappa values, and percentage agreements in percentiles provide little guidance for intervention for adolescents because they just describe the stability of relative rank/position and fail to address the overall change in test scores relative to Fitnessgram Healthy Fitness Zone (HFZ). Fitness tracking studies providing more meaningful information are needed.

PURPOSE: To examine odds ratios and sex differences in staying in the at-risk groups among middle school children across a 31-month period.

METHODS: Fitnessgram battery was administered to 87 boys (mean age = 11.55 ± .54) and 107 girls (mean age = 11.34 ± .48) at baseline and a follow-up with a 31-month interval to assess percent body fat (%BF), BMI, Progressive Aerobic Cardiovascular Endurance Run (PACER), curl-up, push-up, and sit and reach. Based on sex- and age-specific criteria for Fitnessgram hfz, the participants were categorized into an at-risk group (not in HFZ) and a HFZ group (in HFZ) at the baseline and the follow-up for each fitness measure. Odds ratios and sex differences in staying in the at-risk groups across the 31-month period was examined with crosstabs.

RESULTS: Odds ratios of staying in the at-risk groups at the follow-up in fitness measures ranged from 2.6 (95% CI: 1.3-5.1) to 20.4 (95% CI: 7.7-84.7) for those initially in the at-risk groups relative to those who were in the HFZ groups at baseline. Across the 31 months the numbers or percentages of boys in the at-risk groups decreased in %BF (23 vs. 19, -17%), BMI (28 vs. 24, -14.3%), PACER (39 vs. 31, -20.5%), and curl-up (12 vs. 9, -25%); and remained no change in push-up (31 vs. 31, 0%) and sit and reach (15 vs. 15, 0%). In a sharp contrast with boys, the numbers of girls in the at-risk groups increased in %BF (18 vs. 23, +27%), BMI (29 vs. 35, +20.7%), PACER (24 vs. 39, +62.5%), and push-up (38 vs. 51, +34.2%); and decreased in curl-up (24 vs. 11, -54.2%) and sit and reach (47 vs. 29, -38.3%).

CONCLUSIONS: Children of roughly 11 years old who are at risk in fitness levels are much more likely to be in the at-risk groups later relative to those who are in the HFZ groups at baseline. In addition, during the middle school period more girls tend to become at risk in fitness levels, whereas numbers of boys in the at-risk groups tend to decrease.

1270 Board #51 MAY 30 11:00 AM - 12:30 PM

Construction of Delayed Menarche Evaluation in Korean Female Athletes

Katsunori Fujii, FACSM. Aichi Institute of Technology, Toyota, Japan.

(No relationships reported)

PURPOSE: In the present study we conducted a regression analysis of age at menarche against age at maximum peak velocity (MPV) of height in non-athlete Korean girls (control group), and composed 1st to 3rd order regression polynomials to obtain the best regression polynomial. Author then applied the age at menarche and age at MPV of height of athletes to the best polynomial regression evaluation, and investigated the validity of a delayed menarche evaluation system that we constructed.

Method: The subjects were 150 second grade female students at a physical education high school in the suburbs of Pusan, South Korea. A questionnaire survey of these girls was conducted, from which their date of birth, age at menarche, and athletic activities in elementary, junior high school, and high school were obtained. In addition, health check records were examined retrospectively, and longitudinal growth data for height were obtained from the 1st grade of elementary school (7 years old) until the second year of high school (17 years old). One hundred twenty-four girls for whom all data were available were selected. Next, the same survey as above was also done for second grade students at a general high school in the same area, as a control group. Three hundred forty-five non-athletes for whom all data were available were selected. In the analysis, first the wavelet interpolation method was applied to the longitudinal height growth data for the athlete and control groups, and age at MPV was determined from the velocity curve.

RESULTS: The third order polynomial was found to be most suitable for the regression polynomial. When it was applied to individual female Korean athletes with respect to the regression evaluation, positive scores were obtained for nearly all athletes and an overall delay in menarche was seen. Delayed menarche was not seen, however, in archery athletes. A strong delay in menarche was thus found in Korean athletes.

CONCLUSION: it was found that 80% had moderate or greater menstrual pain and a close relation with menstrual abnormalities, suggesting that delayed menarche may be a barometer for menstrual abnormalities. The demonstration of delayed menarche in Korean female athletes from the above suggests the validity of the delayed menarche evaluation.

1271 Board #52 MAY 30 11:00 AM - 12:30 PM

Cardiovascular Fitness and Bone Health of Adolescent Girls According to Age of Menarche

Rebecca A. Battista, Lanay M. Mudd. Appalachian State University, Boone, NC. (Sponsor: Alan Utter, FACSM)

(No relationships reported)

The teenage years are one of the most critical stages of bone mineral acquisition. Maturation may also be related to changes in cardiovascular fitness. However, little is known about the relations among menarcheal status, cardiovascular fitness and bone health during this timeframe.

PURPOSE: The purpose of this study was to determine relations among menarcheal status and cardiovascular fitness, bone mineral content (BMC) and bone mineral density (BMD) among teenage girls in the National Health and Nutrition Examination Survey (NHANES) 1999-2004.

METHODS: Participants included girls with complete dual-energy X-ray absorptiometry (DEXA) measures aged 12 to18 years who had experienced menarche (N = 1458). Menarcheal status was defined as: early maturers (EM) with menarche < 12 years; average maturers (AM) with menarche 12-14 years (referent); and late maturers (LM) with menarche > 14 years. Self-reported demographics and physical activity (PA), measured height and weight (body mass index calculated), predicted VO2max from a cycle ergometer test, and BMC, BMD, and percent fat from a DEXA were obtained. SAS v9.2 used weighted analyses to account for the complex sampling frame of NHANES. Linear regression analyses were used to assess relations among menarcheal status, VO2max, BMC, and BMD.

RESULTS: EM were significantly (p<0.05) younger (mean ± standard error: 14.7±0.1, 15.4±0.1, 16.6±0.1 years), taller (160.7±0.4, 161.9±0.3, and 163.4±0.5 cm), and larger (24.1±0.4, 23.1±0.3, 22.1±0.5 kg/m2; 34.3±0.4, 33.2±0.5, 31.8±0.6 %fat) compared to the AM and LM, respectively. Menarcheal status was not related to VO2max in unadjusted or adjusted analyses. After adjustment for age, race and total minutes of PA, EM was significantly related to a higher mean BMC (beta estimate ± standard error: 51.8 ± 24.3 g, p<0.05) and tended to be related to slightly higher BMD (0.015 ± 0.007 g/cm2, p=0.054) compared to AM. LM was not related to any changes in BMC or BMD compared to AM.

CONCLUSIONS: EM have a larger body size than AM and LM, and slightly higher BMC and BMD. However, despite their larger body size, fitness levels are similar. Additional research is needed to determine if menarcheal status is related to cardiovascular fitness and bone health later in life.

1272 Board #53 MAY 30 11:00 AM - 12:30 PM

Longitudinal Relationships Between Health-related Physical Fitness Test Item Performances In Elementary-aged Children

Loran D. Erdmann, Cathy S. McMillan. Western Illinois University, Macomb, IL. (Sponsor: Michael P. Godard, FACSM)

(No relationships reported)

Longitudinal study of health-related physical fitness (HRPF) in children is limited.

PURPOSE: To longitudinally examine relationships between HRPF tests in young children.

METHODS: HRPF test data were collected annually from one school district, over an 18-yr period, and included: 1-mile run/walk (MR, in elapsed time); double-leg sit-and-reach (SR); pronated-grip pull-up (PU); and 1-min sit-up (SU). Data were retained from 167 boys and 165 girls who were (a) baseline tested as 5- or 6-yr-old kindergartners, (b) subsequently tested in each of grades 1, 2, 3, 4, and 5; and (c) had complete data at all grades. Pearson’s r correlations were used to examine relations of MR to SR and SU performances, as well as relations of SR to SU performances. Spearman’s rho correlations were used to examine relations of PU to MR, SR, and SU performances, as PU data were nonparametric. All analyses were sex-and-grade specific. P values < .05*, .01**, and .001*** are reported.

RESULTS: Correlations between HRPF test items at grades K, 1, 2, 3, 4, and 5 respectively are as follows: MR/SR: Boys: -.03, -.08, -.13, -.19*, -.08, -.13; Girls: -.09, .05, -.24**, -.19*, -.33***, -.12; MR/SU: Boys: -.37***, -.29***, -.29***, -.27***, -.36***, -.43***; Girls: -.42***, -.29***, -.41***, -.38***,-.45***,-.52***; SR/SU: Boys: .08, .18*, .13, .19*, .30***, .19*; Girls: -.01, .20**, .20**, .15, .26**, .29***; Boys: PU/MR: -.28***, -.34***, -.44***,-.29***,-.40***, -.53***; Girls: -.25**, -.28***, -.26**, -.27**, -.42***, -.39***; PU/SR: Boys: .20*, .21**,.20*, .11, .17*, .14; Girls: .26**, .02, .16*, .21**, .21**, .06; PU/SU: Boys: .30***, .38***, .31***, .43***, .32***, .41***; Girls: .20**, .27***, .49***, .35***, .34***, .32***.

CONCLUSION: For boys and girls across the elementary grades, there are weak to moderate significant inverse relations of MR times to both SU and PU performances and weak to moderate positive relations of PU to SU performances. Although SR performance is significantly related to other HRPF test performances at some grades for both genders, the strength of these relations is only very weak to weak. For these HRPF test item pairs, generally the relational direction remains consistent and the relational strength changes little as young children progress through elementary school.

1273 Board #54 MAY 30 11:00 AM - 12:30 PM

Physical Self-Perception and Physical Fitness in Adolescents With and Without Autism Spectrum Disorders

Chien-Yu Pan1, Chia-Hua Chu1, Chia-Liang Tsai2, Kai-Wen Hsieh1, Ya-Lin Li1, Shih-Tse Huang1. 1National Kaohsiung Normal University, Kaohsiung, Taiwan. 2National Cheng Kung University, Tainan, Taiwan.

(No relationships reported)

Physical self-perception (PSP) exerts a crucial role during adolescence. Research has demonstrated that positive associations of PSP with physical fitness (PF) (Carraro, Scarpa, & Ventura, 2010). However, social and behavioral deficits and sometimes motor skill difficulties demonstrated by individuals with autism spectrum disorder (ASD) could limit opportunities for them to successfully participate in physical activity, and therefore, may put them at risk for being physically inactive and unfit.

PURPOSE: (a) To compare PSP and PF in adolescents with and without ASD, and (b) to assess the relationship of PSP with PF within each group.

METHODS: Thirty-one males with ASD and 36 males without ASD aged 12-18 (14.68±1.55 yrs) participated. The PSP was assessed with a questionnaire originally developed by Whitehead (1995) and translated into Chinese with a high degree of reliability and validity (Hagger, Biddle, Chow, Stambulova, & Kavussanu, 2003). The BROCKPORT Physical Fitness Test was used for PF measures. Independent t tests were used to compare group differences in PSP and PF. Pearson product-moment correlation coefficients were calculated to evaluate the relationships of PSP with PF within each group. Significance was set at p<0.05.

RESULTS: Findings indicate that (a) adolescents with ASD perceived significantly poor scores on physical condition (-2.47, p<0.05), sport competence (-2.05, p<0.05), and general self-worth (-1.53, p<0.05), (b) adolescents with ASD demonstrated significantly poor scores on 20-m PACER (-23.81, p<0.01), isometric push-up (-8.62, p<0.01), and back-saver sit-and-reach (-8.00, p<0.01), (c) for adolescents with ASD, physical condition was associated with 20-m PACER (r=0.58, p<0.01); sport competence was associated with sit-up (r=0.37, p<0.05), and (d) for adolescents without ASD, physical condition (r=0.51, p<0.01) and sport competence (r=0.41, p<0.05) were associated with 20-m PACER; attractive body was associated with sit-up (r=0.35, p<0.05); general self-worth was associated with sit-and-reach (r=0.39, p<0.05).

CONCLUSION: Adolescents with ASD perceived less favorable PSP than adolescents without ASD. PSP and PF indicators were related, despite incomplete overlap between the two variables in each group.Supported by Taiwan NSC grants 99-2410-H-017-036-MY2.

1274 Board #55 MAY 30 11:00 AM - 12:30 PM

Faster Heart Rate Recovery and Higher Short-term Muscle Power in Physically Active Preschool Children

Leigh Gabel, Nicole A. Proudfood, Joyce Obeid, Brian W. Timmons. McMaster University, Hamilton, ON, Canada.

(No relationships reported)

Aerobic fitness has a protective effect on metabolic and cardiovascular health and is positively related to physical activity in youth and adults. There is a paucity of literature examining fitness in preschool children (3- to 5-year olds); thus, little is known about the relationships between physical activity and fitness in this young age group.

PURPOSE: To investigate the relationships between physical activity patterns and fitness (aerobic and short-term muscle power) in a sample of preschool children.

METHODS: Ninety-six preschoolers (4.4 ± 0.9 years; 50 female) participated in the study. Children performed a maximal treadmill assessment (Bruce Protocol) with heart rate (HR) monitored throughout the test and into 1-min of recovery (HR60secpost). Aerobic fitness was assessed by heart rate recovery (HRR = HRmax - HR60secpost). Short-term muscle power was determined by peak power output using a modified 10-sec Wingate cycling protocol. Physical activity patterns were assessed by the duration and frequency of bouts of moderate-to-vigorous physical activity (MVPA) using accelerometry for seven consecutive days with 3-second epochs. Only children who achieved a HRmax of ≥180 bpm on the treadmill test and who wore the accelerometer for ≥3 days for ≥5 hours/day were included in analyses. Pearson correlation coefficients were used to assess the relationships between physical activity and fitness. Both physical activity and short-term muscle power were significantly related to age; thus, partial correlations controlling for age were used.

RESULTS: Average HRmax on the treadmill test was 195 ± 7 bpm with a HRR of 63 ± 14 bpm. HRR was positively related to the frequency of bouts of MVPA (r=0.29, p<0.01). Average peak power on the modified Wingate test was 4.6 ± 1.5 Watts/kg and was positively related to the duration of bouts of MVPA (r=0.23, p=0.03).

CONCLUSIONS: Preschool children who engage in more frequent bouts and longer duration of bouts of MVPA have faster HRR and higher short-term muscle power, respectively, compared to preschoolers who engage in less frequent and shorter duration of bouts of MVPA.

1275 Board #56 MAY 30 11:00 AM - 12:30 PM

Characteristics of Physical Fitness According to the Level of Obesity Index among Young Children

Kosho Kasuga1, Takahiro Nakano2, Kazuo Oguri3, Keisuke Fukutomi4. 1Gifu University, gifu, Japan. 2Nagoya Gakuin University, seto, Japan. 3Shizuoka Sangyo University, iwata, Japan. 4Gifu Sports Science Training Center, gifu, Japan. (Sponsor: Kiyoji Tanaka, FACSM)

(No relationships reported)

Many reports have shown that the prevalence of obesity in Japanese children, including infants, has been increasing. The number of children diagnosed with infant metabolic syndrome is also on a rise. Moreover, in recent years, there has been an increase in the number of lean children in Japan.

PURPOSE: The purpose of this study is to examine the characteristic of physical fitness according to level of obesity index among young children (age, 3-6 years) by using a large dataset.

METHODS: This study included 5640 young children (2840 boys and 2800 girls). We administered physical fitness tests comprising 7 exercises to gauge the physical fitness status of these children. A T-score was calculated on the basis of the mean and standard deviation according to sex and age; this score was used as an individual score of physical fitness. The children were divided into 7 groups based on their obesity index. Further, the extent of differences among children with different obesity index was examined for each type of exercise. To conduct a statistical analysis of the data, one-way ANOVA and multiple comparisons (Tukey’s HSD test) were employed.

RESULTS: Statistical analysis showed a significant difference among the groups for all the exercises. The children in the “+20% or more” group had the lowest scores in 25-m run, standing long jump, and upright hand standing time. The children in the “-15% or less” group had the lowest scores in softball throw, sitting trunk flexion, side-step, grip strength, and overall physical fitness.

CONCLUSIONS: The physical fitness of obese (+20% or more) and lean (-15% or less) children was found to be poor. The poor physical fitness in lean children may be due to low muscle mass and underdevelopment because of the lack of any physical activity. Moreover, their low energy expenditure may result in a low appetite, which can lead to poor nutrition. In contrast, the severely obese children (+20% or more) may be underdeveloped due to the lack of any physical activity and consumption of an excessive and unbalanced diet. However, the mildly obese (up to +15%) are not physically underdeveloped at infancy, and do not need extreme obesity prevention measures. It is important for them to adopt a “play a lot, eat a lot” lifestyle.

1276 Board #57 MAY 30 11:00 AM - 12:30 PM

The Relationship Between The Development Of Physical Fitness And Lifestyle Behavior In Young Children

Takahiro Nakano1, Kosho Kasuga2, Kazuo Oguri3. 1Nagoya Gakuin University, Aichi, Japan. 2Gifu University, Gifu, Japan. 3Shizuoka Sangyo University, Shizuoka, Japan. (Sponsor: Kiyoji Tanaka, FACSM)

(No relationships reported)

Recently, the decline in children’s physical fitness has become a big problem in Japan for not only older children but also young children. Childhood is very important period for acquisition of basic lifestyle behavior. Many studies have shown a relationship between physical fitness and lifestyle behavior. However, few studies have examined the relationship between the development of physical fitness and lifestyle behavior. Such studies have been particularly rare in young children.

PURPOSE: The purpose of this study was to examine the relationship between the development of physical fitness and lifestyle behavior in young children using longitudinal data.

METHODS: The subjects of this study were 311 young children. We measured seven parameters related to physical fitness: grip strength, upright hand standing time, sitting trunk flexion, 25-meter run, standing long jump, side step, and softball throw. As well, we administered a questionnaire about daily lifestyle behavior. We calculated the development of physical fitness using two-years longitudinal data for the same young children, and calculated the correlation coefficient between the amount of development and daily lifestyle behavior. Further, we examined differences in lifestyle behavior between groups whose physical fitness developed well or did not, using a chi-square test and independent t-test.

RESULTS: The lifestyle behaviors related to several physical fitness items were “Bedtime,” “TV viewing time,” “video-game playing time,” “active play with body movement” and “active play with family”. Significant differences were confirmed between the high-development group and the low-development groups in 7 items, including “TV viewing time,” “video-game playing time,” “number of friends usually playing together,” and “keeping a regular lifestyle”.

CONCLUSION: Relationships between young children’s physical fitness and several lifestyle behaviors were confirmed. It is suggested that physical fitness is easy to develop in children who have a regular lifestyle and a lot of friends to play with. In addition, it is suggested that the development of young children’s physical fitness is promoted by improving lifestyle behaviors such as TV viewing time and video-game playing time.

1277 Board #58 MAY 30 11:00 AM - 12:30 PM

Use Of Exergaming For Obtaining Moderate And Vigorous Physical Activity In Children

Magdalene Horton, Larry Kennard, Scott E. Crouter, FACSM. University of Massachusetts Boston, Boston, MA.

(No relationships reported)

There are a growing number of PA programs that now include interactive video games (exergames) as part of an activity routine; however, it is unclear, if these games are a viable alternative for children obtain moderate and vigorous PA (MVPA).

PURPOSE: The purpose of this study was to examine the energy cost of children playing exergames. A secondary purpose was to see how the energy cost of exergames compares to other commonly performed activities.

METHODS: Fifty-three boys and 50 girls (mean±SD; age 11.5±1.7 yrs; BMI%ile, 68.6±28.4 %) performed 30-min of lying rest. Each participant was then randomly assigned to perform one of three activity routines consisting of six activities. Participants were familiarized with all activities prior to testing. Exergames included were: Nintendo Wii, Floor-based LightSpace, Wall-based LightSpace, Dance Dance Revolution, SportWall, and Cybex Trazer. Oxygen consumption was measured using a Cosmed K4b2. Measured METs (measured activity VO2/measured resting VO2) were calculated for each activity. Data were also examined for differences between genders, age groups (8-9, 10-11, and 12-15 yrs) and BMI [normal weight (<85th% for age and sex), overweight (85th-95th% for age and sex), and obese (≥95th% for age and sex)].

RESULTS: On average, the mean METs for playing the Nintendo Wii were 2.5 METs. All other exergames had a mean MET value of at least moderate intensity (range; 3.4-5.3 METs). In general, there were no differences by gender, age, or BMI categories, except for; Nintendo Wii (overweight, 3.6 METs vs. normal weight and obese, 2.6 METs), Cybex Trazer (9 yrs, 2.5 METs vs. 10-11 yrs 3.9 METs vs. ≥12 yrs 5.0 METs) and SportWall (8-9 yrs, 2.7 METs vs. 10-11 yrs 4.5 METs vs. ≥12 yrs 6.9 METs). Playing exergames resulted in similar MET values as other commonly performed PA in children (e.g., slow and brisk track walking, track running, playing catch, and soccer)

CONCLUSION: The results of this study show that, in general, the energy cost to play exergames is at a moderate intensity or greater. Exergames could be an alternative way for children to obtain MVPA in addition to the use of more traditional activities. However, there may issues related to age and BMI that need to be further investigated as not all activities work equally well for all individuals.

Study supported by NIH grant 5R21HL093407-02

1278 Board #59 MAY 30 11:00 AM - 12:30 PM

Compliance with Physical Activity Recommendations and Blood Pressure In Preschool Children

Jorge Mota, Sandra Santos, Rute Santos, Luisa Soares-Miranda, Carla Moreira, sandra Abreu, Susana Vale. Research Centre in Physical Activity health and Leisure, Porto, Portugal.

(No relationships reported)

PURPOSE: Research suggests that moderate levels of PA are predictors of lower values of systolic and diastolic blood pressure (SBP and DBP). However, few studies addressed this issue in pre- school children. The purpose of this study was to analyse the associations between compliance of PA recommendations and SBP preschool children.

METHODS: The sample comprised 593 preschool children (48% female) with mean age of 5.3 years. Physical activity was assessed during 7 consecutive days by accelerometer (Actigraph GTM1). We analyzed the recommendation of at least one hour daily of moderate-vigorous PA (MVPA). Blood pressure was measured using the Colin monitor. The measurements were performed with each subject seated and rested for at least five minutes before the test. Percentage of fat (% MG) was calculated using the equation of Westrate and Durenberg. (1990).

RESULTS: The prevalence of BP above the 90th percentile (P90) was 7.8% and 1.3% respectively for SBP and DBP. We found that about 16.5% of the girls and 8.2% of the boys did not accomplish the recommended daily MVPA. Girls who did not meet the daily recommendations of MVPA were twice as much more likely to have SBP values above the P90 compared to those who meet the daily recommendations (OR: 2.6; CI 95%:1.0-6.6; p<0.05), even after adjustment to fat mass.

CONCLUSION: We found an association between the accomplishment of daily PA recommendations and SBP in pre-school children in girls.

1279 Board #60 MAY 30 11:00 AM - 12:30 PM

After-School Exercise Increases Endothelial Progenitor Cells in Overweight and Obese Children

Jong-Hwan Park1, Masashi Miyashita1, Yoshio Nakamura1, Hyun-Tae Park2, Yoo-Chan Kwon3, Eun-Hee Kim3, Jin-Kee Park3, Ki-Beam Park3, Sang-Kab Park3. 1Waseda University, Saitama, Japan. 2National Center for Geriatrics and Gerontology, Aichi, Japan. 3Dong-A University, Busan, Korea, Republic of.

(No relationships reported)

Obese children who are home alone after school have limited time to be active outdoors and unlimited access to junk food, video games and electronic devices which can lead to a more sedentary lifestyle. The role of bone marrow-derived circulating endothelial progenitor cells is to maintain endothelial function and organ perfusion. Endothelial dysfunction is associated with the childhood obesity and is closely linked to the amount and function of endothelial progenitor cells. However, it remains unclear whether endothelial progenitor cells increase with after-school exercise in overweigh and obese children.

PURPOSE: The purpose of this study was to investigate the effects of an after-school exercise programme on endothelial progenitor cells in overweight and obese children.

METHODS: Total of 29 overweight/obese children were randomly divided into the control (i.e., no after-school exercise, n = 14) or after-school exercise (n = 15) groups. The 12-week after-school exercise intervention consisted of 3 days of combined aerobic and resistance exercise per week. Each 80-minute exercise programme included 10 minutes of warm-up and 10 minutes of cool-down after school. CD34+ (a cell surface marker on hematopoietic stem cells), CD133+ (a cell surface marker on hematopoietic progenitor cells) and CD34+/CD133+ (considered as endothelial progenitor cells) were measured at baseline and after 12 weeks using flow cytometry.

RESULTS: Increased percentages of CD34+, CD133+ and CD34+/CD133+ were observed in the after-school exercise group (p = 0.018; p = 0.001; p = 0.002, respectively) compared with the control group. Carotid intima-media thickness decreased after 12 weeks in the after-school exercise group (p = 0.020) compared with the control group. Circulating concentrations of vascular endothelial growth factor and nitric oxide were increased in the after-school exercise group (p = 0.013; p = 0.005, respectively) compared the control group.

CONCLUSIONS: This study provides preliminary evidence that an after-school exercise programme may represent an effective intervention strategy for improving vascular repair and endothelial function, leading to improved cardiovascular health in overweigh and obese children.

1280 Board #61 MAY 30 11:00 AM - 12:30 PM

Physical Activity, Focused Education and Endothelial Function in an Obese Teen: A Case Study

Anna Gabbard, Nabil Boutagy, Elise Maniguet, Alison Peters, Shannon Isom, John W. Wygand, Robert M. Otto, FACSM. Adelphi University, Garden City, NY.

(No relationships reported)

Endothelial function plays a significant role in vascular health. Normal endothelial function includes release of vasoactive substances and growth factors to maintain vascular homeostasis. Endothelial dysfunction (Reactive Hyperemia Index [RHI] < 1.67) can be defined as a loss of balance between vasoconstrictors vs vasodilators and other inflammatory factors. Endothelial dysfunction is precursor to atherosclerosis and is implicated in obesity, diabetes and hypertension. EndoPAT noninvasively assesses endothelial function by reactive hyperemia- pulse amplitude tonometry (RH-PAT). Endothelial function is calculated by measuring blood vessel diameter relative to resting, occluded and hyperemic conditions.

PURPOSE: To determine whether a combined education and physical activity program has an effect on endothelial function in an obese female(age 18 yr, wt 108 kg, ht 170 cm, BMI 37.4 m/kg2).

METHODS: EndoPAT was performed in a thermo-neutral environment with the subject supine and both index fingers in the EndoPAT probe. A blood pressure cuff (BPc) was placed on the subject’s non-dominant arm. Baseline data were collected for 5 minutes. The BPc was inflated to ≥200 mmHg for complete occlusion for 5 minutes, rapidly released and post-occlusion data were collected. Body composition was measured by skinfolds & waist to hip (W/H) with Gulick tape. The intervention was a 12 week program of nutrition and physical activity education combined with twice weekly exercise sessions designed to reduce obesity and modify the participant’s obesogenic environment. Each 60 minute exercise session, consisted of 20-35 minutes of aerobic interval cross training on 4-7 exercise machines at 65-85% HRR for 5 min. each. A circuit of resistance training was performed on 12 Nautilus resistance machines to an intensity of momentary muscular fatigue.

RESULTS: The subject reduced body mass 10.8%, BMI 10.9%, body fat 7.3%, W/H 2.5%, and improved muscular strength 79% (10 RM leg press). RHI improved from dysfunction of 1.46 at baseline to normal function of 2.61 following intervention.

CONCLUSION: A cardiovascular and resistance training intervention improved endothelial function, body composition and muscular strength in an obese teen and attenuated cardiovascular risk.

1281 Board #62 MAY 30 11:00 AM - 12:30 PM

Comparison Between Individual And Averaged Methodologies For Anaerobic Threshold Assessment Of Age-group Swimmers

Marisa Sousa, João Paulo Vilas-Boas, Ricardo Fernandes. University of Porto, Faculty of Sports, Porto, Portugal. (Sponsor: Carlo Baldari, FACSM)

(No relationships reported)

The assessment of the velocity corresponding to anaerobic threshold (vAnT) is frequently part of elite swimmers training program in order to diagnosis their aerobic performance. However, the velocity corresponding to blood lactate concentration ([La-]) of 4 mmol/l (v4), an averaged value proposed for standard more than 30 years ago, is often used in detriment of individualized protocols; in addition, rarely swimmers of young ages are involved in training control.

PURPOSE: To compare the vAnT of 10-11 years old swimmers assessed by individualized protocols (step and critical velocity tests) and by the traditional v4 value.

METHODS: Fifteen age-group swimmers (10.7±0.70 years) performed a 5×200 m front crawl step test (increments of 0.05 m/s per step, and 1 min rest intervals), being capillary blood samples for [La-] analysis collected from the earlobe at rest, after each step and at the end of the test, which allowed the assessment of the vAnTStep through [La-]/velocity curve modeling method, and v4 by linear interpolation. Critical velocity (CV) was obtained through the slope of the linear relationship of time versus distance based on the times of the 100 and 400m competitive events. Mean and SD were computed, as well as repeated measurements ANOVA (with Bonferroni post hoc test) and Pearson correlation coefficient (p≤0.05).

RESULTS: Mean±SD values of vAnTStep, CV and v4 were 1.03±0.05, 1.03±0.07 and 1.08±0.06 m/s, respectively, being the two former velocities significantly lower than v4; these differences corresponds to a 5 s gap in a 100m front crawl effort. The [La-] corresponding to AnT obtained in the step test was 2.3±0.59 mmol/l.

CONCLUSIONS: v4 does not represent the individual vAnT in children involved in competitive swimming, and both a 200 m step incremental protocol, with the later determination of the precise point of the rise in [La-], and CV, seems to be advised for individual anaerobic threshold assessment in young swimmers.

Supported by FCT PTDC/DES/101224/2008 (FCOMP-01-0124-FEDER-009577)

1282 Board #63 MAY 30 11:00 AM - 12:30 PM

Validity of an Alternate Knee Raise Test for Assessing Aerobic Fitness of Adolescents

Stanley Sai-chuen Hui, FACSM, Wing-luen Au, Lin Wang, Wing-yee Chan, Ka-wai Chan. The Chinese University of Hong Kong, Shatin, Hong Kong, Hong Kong.

(No relationships reported)

PURPOSE: Simple but valid home-based aerobic fitness test that can be self-administered with minimal equipment is lacking. The purpose of this study was to examine the validity of an alternate knee raise test (AKR) for assessing aerobic fitness of adolescents.

METHODS: With informed consent form completed, 30 male adolescents age 12 to 18 yrs-old (14.63 ± 1.56 yrs-old) volunteered to participate in an AKR test and a grade maximal treadmill exercise (GXT) test. In the AKR test, participants were asked to perform alternate knee raise movement in place following a cadence of 96 beats/min for 3 mins continuously. In each knee raise motion the participants were required to lift up their leg until their thigh touched their palm which was placed in front of their waist at the same level of iliac crest and above their thigh. The movement was similar to a platform step test but no platform was required. At the end of the 3 min AKR test, the post-exercise heart rate (PHR) at 20 seconds after the test was recorded by a Polar heart rate monitor. For GXT test, criterion measure of VO2max was examined by a portable COSMED K4b2 metabolic analyzing system. To evaluate the predictive validity of AKR test, independent variables of age, body weight, skinfolds, body mass index, resting heart rate, post-exercise heart rate were entered into stepwise regression procedures for predicting dependable variable of measured VO2max.

RESULTS: Regression analysis revealed that the AKR test produced good predictive validity and acceptable standard error of estimates. Only two independent variables were needed which were age and PHR. The suggested equation was VO2max (ml/kg/min) = 52.168 - 0.25 (PHR) + 1.568*(Age), R=0.71, SEE=5.17 ml/kg/min.

CONCLUSIONS: The AKR Test was found to be a valid submaximal field test for assessing aerobic fitness of adolescents. It is a practical test due to its’ simple procedure and can be self-administered at home with minimal equipment.

1283 Board #64 MAY 30 11:00 AM - 12:30 PM

Body Perception: Parents’ Poorly Perceive Children’s Fitness Characteristics

Elan J. Lieber, Carmen B. Swain. The Ohio State University, Columbus, OH.

(No relationships reported)

At least 155 million school-age children worldwide are overweight or obese. Body Mass Index (BMI) as a measurement of parental recognition of child’s overweight status ranges from 6-73%. When examining overweight children, less than 50% of parents identify their child as such.

PURPOSE: The purpose of this study was to examine parental ability to appropriately identify their child’s BMI status and also measures of physical fitness (body composition, muscular strength, flexibility and aerobic fitness).

METHODS: Subjects consisted of 133 males (10.8±2.1 years), 110 females (11.4±2.4 years) and their maternal parent. Males and females scores were compared to maternal perception of score: BMI, body composition, and fitness characteristics (muscular strength, aerobic fitness and flexibility) were categorized according to normative rankings. The maternal perception questionnaire was administered while subject’s fitness characteristics were evaluated. Data associations were examined using descriptive statistics and bivariate correlation.

RESULTS: BMI, body fat and fitness characteristics ranged categorically from poor to excellent. Maternal parents ability to correctly identify fitness characteristics by categorical selection were: BMI (72.8%), body composition (49.4%), flexibility (26.3%), muscular strength (32.5%) and aerobic fitness (39.5%). The relationship between subjects scores and parental perception of scores was considered weak to moderate, as determined by Pearson correlation (BMI: r=.618, body composition: r=.693, muscular strength: r=.268, aerobic fitness: r=.406 and flexibility r=.435).

CONCLUSION: In children ranging from under-weight to obese, parent’s ability to predict characteristics relative to weight and body composition is substantially better than their ability to predict fitness characteristics. Parents have a strong influence on children’s behavior and physical activity levels. Primary prevention is key in fighting the obesity epidemic, thus adult’s awareness of their child’s fitness status is imperative. As such, professional or school-based screening could be suggested as a necessary tool to identify children’s fitness characteristics for parental informative purposes.

1284 Board #65 MAY 30 11:00 AM - 12:30 PM

Prevalence Of Obesity In Japanese Children Aged 3.5 Through 6.5 Years Old

Kazuo Oguri1, Kosho Kasuga2, Takahiro Nakano3. 1Shizuoka Sangyo University, Iwata, Shizuoka, Japan. 2Gifu University, Gifu, Japan. 3Nagoya Gakuin University, Aichi, Japan. (Sponsor: Kiyoji Tanaka, FACSM)

(No relationships reported)

The prevalence of childhood obesity has risen progressively worldwide. It is said that obesity onsets in early childhood; however, factors not yet clarified include: age of obesity onset, and age when gender difference in obesity prevalence becomes apparent.

PURPOSE: The purpose of this study was to examine the prevalence of obesity and its development trends, such as differences with age and gender, in Japanese young children.

METHODS: This cross-sectional study analyzed height, weight and age data of 6,235 children (3,147 boys and 3,088 girls) aged 3.5 to 6.5 years old. Subjects were categorized into age groups in increments of 0.5 years, and prevalence of obesity was compared between gender and among groups. Obesity was defined according to the National Health and Nutrition Survey of Japan as 115% of the standard weight for an individual’s height and gender. The chi-square test was used to compare differences in prevalence of obesity. Where appropriate, p values of the chi-square test were corrected according to Bonferroni inequalities.

RESULTS: Prevalence of obesity plateaued at 6.4 % to 6.7 % both in boys and girls, aged between 3.5 years and 4.5 years. However, it significantly decreased to 3.1 % and 3.0 % in boys and girls respectively, at age 5.0 years old (p<0.001). Prevalence of obesity in boys and girls aged 5.5 years increased to 8.0 % and 6.8 % respectively, which was significantly higher than that in boys and girls at 5.0 years old, and the rate gradually increased with age thereafter (p<0.001). No significant difference in prevalence of obesity was found between boys and girls in each group for ages 3.5 years to 6.0 years. However, the prevalence was significantly higher in boys than in girls at age 6.5 years (p<0.05).

CONCLUSIONS: Our data suggest that prevalence of obesity temporarily decreases at age 5.0 years old, however, it markedly increases from age 5.5 years old. Prevalence of obesity among boys significantly increases relative to that among girls at age 6.5 years. These findings indicate that prevention and treatment of young childhood obesity is important for pediatric healthcare.

1285 Board #66 MAY 30 11:00 AM - 12:30 PM

Relationship of Body Mass Index to Exercise Participation and Adherence in Overweight Children and Adolescents

Amanda Gier, Christopher Kist, Wayne A. Mays, Robert Siegel, Shelley Kirk. Cincinnati Children’s Hospital Medical Center, Cincinnati, OH.

(No relationships reported)

PURPOSE: To determine if body mass index (BMI) in overweight and obese children and adolescents is a barrier to participation in and adherence to an organized group exercise program.

METHODS: During medical visits for a clinical pediatric weight management program, height and weight was obtained on 603 overweight children and adolescents and used to calculate BMI. Patients were given information about the group exercise sessions available to them through the program. Group classes were offered five evenings per week, and participants could attend at their discretion. Classes were 1-hour long and consisted of strength training, cardiovascular activity and active games in a fun, non-competitive environment. Class attendance was tracked and duration of active participation was determined. Data was analyzed to determine if BMI was related to class attendance.

RESULTS: Active class attendance ranged from 0 to 182 weeks. There was no correlation between BMI (34.9 ± 7.5 kg/m2) and weeks attended (10.7 ± 22.4 weeks) (r= -0.054, NS) when looking at all participants. Within the group of participants that attended group exercise sessions, N=355, there was no correlation between BMI (34.4 ± 7.8 kg/m2) and total weeks of exercise class attendance(18.1 ± 26.8 weeks) (r= -0.03,NS). However, when comparing the participants that attended classes to those who did not attend any classes, the children who did attend classes had a significantly lower BMI (34.4 ± 7.8 vs 35.6 ± 7.1 kg/m2) (p<0.05), but were also significantly younger (11.2 ± 3.3 vs 12.0 ± 3.3 years) (p<0.005).

CONCLUSION: BMI was not related to the length of time that children actively participated in group exercise sessions. However, BMI and age were significantly lower in the group that attended classes. Younger children may have a lower BMI but still be in a higher BMI percentile for age than older children with a similar BMI. Therefore, BMI alone may not be helpful in determining which patients will participate in an exercise program. Future research should focus on a multivariate analysis of BMI percentile and age as indicators of participation in exercise classes. Further research into these variables could give healthcare providers insight into which patients may need additional support and encouragement when beginning structured exercise.

1286 Board #67 MAY 30 11:00 AM - 12:30 PM

Relationship between Health Status and Motor Ability among Young Children

Keisuke Fukutomi1, Kosho Kasuga2, Katsunori Fujii3, Tohru Isigaki4. 1Gifu Sports Science Training Center, Gifu, Japan. 2Gifu University, Gifu, Japan. 3Aichi Institute of Technology, Toyota, Japan. 4Aichi Prefectural University of Fine Arts and Music, Nagakute, Japan. (Sponsor: Kiyoji Tanaka, FACSM)

(No relationships reported)

It has been suspected that people with low motor ability are liable to have bad health status. However, less research has been performed on the motor ability of young children by using a large dataset.

PURPOSE: The purpose of this study is to examine the relationship between health status and motor ability among young children.

METHODS: The study included 2,431 young children (1,241 boys and 1,190 girls; age range: 4-6 years) and their parent. We administered motor ability tests comprising 10 exercises to gauge the motor ability status of these children. To obtain the overall motor ability parameter, the measurement items were summarized by using principal component analysis. And we concluded that the first principal component represented the overall motor ability. Further, the principal component score of the first principal component was divided by the number of subjects of each sex and 0.5 years to obtain the individual T-scores. These individual T-scores indicated the motor ability scores. Questionnaires comprising 13 items on health status_frequent loose bowels, frequently catching cold, having a nosebleed, having eczema and suppuration, having car sickness, having no notable symptoms, allergy constitution, asthma, atopy, nasal catarrh, food allergy, drug allergy, and conjunctivitis_were administered to their parent. Student’s t-test was performed to determine the statistical differences in the motor ability scores between children who answered ‘True’ and those who answered ‘False’. The level of significance was set at p < 0.05. The data were collected in 2009.

RESULTS: With respect to the health status, the motor ability scores of the group that answered ‘True’ were significantly lower than those of the group that answered ‘False’ for items under the category ‘having no notable symptoms’ (True: 50.7, False: 49.3) and ‘frequently catching cold’ (True; 47.9, False; 50.3). Particular attention was paid to allergy symptoms. Statistical analysis showed that there was no significant difference in the motor ability scores of young children who answered ‘True’ and those who answered ‘False’.

CONCLUSIONS: The young children who caught cold frequently had low motor abilities. Moreover, 38% of the subjects had allergy constitution; however, presence of an allergy did not have any correlation with motor ability.

1287 Board #68 MAY 30 11:00 AM - 12:30 PM

Relations between Sedentary Behavior and FITNESSGRAM® Healthy Fitness Zone Achievement and Physical Activity

Jacob S. Tucker, Scott B. Martin, Allen W. Jackson, FACSM, James R. Morrow, FACSM, Christy A. Greenleaf, Trent A. Petrie. University of North Texas, Denton, TX.

(No relationships reported)

Sedentary behaviors such as television viewing and video game playing increase the relative risk of morbidity and mortality independent of physical activity levels. Due to the direct relations between these sedentary behaviors and health risk, the American Academy of Pediatrics recommends no more than two hours of daily screen time for youth.

PURPOSE: To investigate the relations between sedentary behaviors and health-related physical fitness (PF) achievement and physical activity (PA) behaviors in middle school students.

METHODS: Students (N=1515) in grades 6-8 completed the FITNESSGRAM® PF items, and FITNESSGRAM PA questions in addition to Youth Risk Behavior Survey sedentary behavior questions as part of school activities.

RESULTS: Using logistic regression and controlling for gender, age, ethnicity, and economic status, we calculated odd ratios (OR) for all components of health-related fitness, aerobic capacity (OR=1.70, 95%CI = 1.30-2.34), muscular strength and endurance (OR = 1.87, 95%CI = 1.30-2.68), flexibility (OR = 1.54, 95%CI = 1.20-2.00), and body composition (OR = 1.71, 95%CI = 1.27-2.30). The odds of achieving the FITNESSGRAM Healthy Fitness Zone were higher when students reported ≤2 hours of sedentary behaviors per day. Similarly, the odds of achieving physical activity guidelines for aerobic (OR = 1.42, 95%CI = 1.09-1.84), muscular strengthening (OR = 1.77, 95%CI = 1.22-2.57), and flexibility (OR = 1.47, 95%CI = 1.15-1.88) activity were higher when students reported ≤2 hours per day of sedentary behaviors.

CONCLUSION: Results illustrate the importance of keeping sedentary behaviors to ≤2 hours per day in middle school students, thus increasing the odds that students will achieve sufficient health-related fitness for health benefits. Less screen time is related to achieving national physical activity guidelines.

Grant support from the National Association for Sport and Physical Education.

1288 Board #69 MAY 30 11:00 AM - 12:30 PM

Associations Among Body Fat %, BMI, And Fitness Test Performance In School-Aged Children

Ryan D. Burns1, James C. Hannon1, Brett Allen1, Pedro F. Saint-Maurice2, Gregory J. Welk, FACSM2. 1University of Utah, Salt Lake City, UT. 2Iowa State University, Ames, IA.

(No relationships reported)

PURPOSE: The purpose of this study was to examine associations among body fat % (BF%), BMI, and various fitness tests in school-aged children.

METHODS: The participants included male and female 4th through 10th grade students (n=376) recruited from three private schools from a metropolitan area in the southwestern United States. Participants completed assessments of body composition, aerobic fitness and muscular fitness on 4 separate testing days with at least 48 hours between sessions. On day 1 participants completed the BMI, BF% and PACER aerobic fitness test. On day 2 participants completed DYNAMIC CURL UPS and ROMAN CHAIR STATIC extension tests. On day 3 participants completed PLANKS, LATERAL STRENGTH, and ROMAN CHAIR DYNAMIC extensions. On day 4 participants completed any test that they may have missed on previous days. Pearson-Product Moment (PPM) correlations were used to assess the degree of association between body composition and fitness variables. Alpha level of 0.05 was set to determine significance for statistical analyses.

RESULTS: The BMI and BF% values were moderately correlated with each other (r=.51, p<.001). BF% had higher correlations with the fitness variables than BMI (PACER: r=-.407 vs. -.094; CURL-UP: r=-.197 vs. .108; PLANK: r=-.311 vs. -.06; LATERAL STRENGTH: r=-.239 vs. -.078; ROMAN CHAIR DYNAMIC: r=-.142 vs. -.128; ROMAN CHAIR STATIC: r=-.149 vs. -.133). BF% correlations were significant among all fitness tests (p<.001) while BMI correlations were only significant for both ROMAN CHAIR extensions (p<.05).

CONCLUSION: The results suggest that BF% is negatively correlated with indicators of aerobic fitness and muscular fitness in youth. The moderate correlations between BF% and BMI and the weaker associations between BMI and fitness performance tests indicate that BMI may provide a less useful indicator of body composition for use in school health related fitness testing.

1289 Board #70 MAY 30 11:00 AM - 12:30 PM

A Comparative Cross-cultural Comparison Of Posture, Core Strength, Height, Age And Body Mass Index

Ulrike H. Mitchell, A Wayne Johnson, Barbara Simmons. Brigham Young University, Provo, UT.

(No relationships reported)

PURPOSE: High income countries around the world have experienced dramatic increases in obesity and sedentary behaviors in both genders, all income levels, ethnic groups, and all ages. We hypothesized that the lack of multiple TV channels or computers would have a positive impact on posture and fitness. This project was conducted in the rural villages outside of Irapuato, Mexico. The data were compared to similar studies conducted in Ghana, Australia and the United States.

METHODS: Intervention: 29 children, ages 10-12, performed 5 exercises indicative of core strength (plank, left and right bridge, left and right stork); the times of how long they were able to hold the positions were documented. With reflective markers placed on bony landmarks, the children had posture assessment photographs taken of their normal standing posture from a lateral view. A software program was used to mark and measure postural angles.

RESULTS: Height and weight (and their SD) were greater in Mexico (139.7cm SD9.6 and 35.1kg SD11.9) than in Australia (138.8cm SD5.8 and 32.4kg SD3.8). The gaze angle was greater in the Hispanic children (14.10 deg SD8.48) compared to the Australian’s (10.3 deg SD7.6); this indicates a greater propensity towards a forward head posture.

The head on neck angle was greater in the Australian children (55.7deg SD8.7) compared to the Hispanics (50.3deg SD6.1); however, we believe that this is the result of a protruding C7 marker rather than an actual postural dysfunction.

Core strength: There were differences in exercise times for the side bridges and stork exercises between the children tested in Mexico and Utah County and for the left side bridge and stork exercises between the children tested in Mexico and Ghana (all with p-values <.05); there was no difference in exercise times between children tested in Ghana and Utah County.

CONCLUSIONS: Our hypothesis was not supported by this study. While life in Irapuato, Mexico, is less influenced by computers and TV, it doesn’t seem to have any impact on better posture or fitness. Reasons for this include widespread poverty which encourages consumption of cheaper high-calorie processed foods, culturally anchored sedentary lifestyle habits, lack of physical and health education and exercise-prohibitive living conditions.

1290 Board #71 MAY 30 11:00 AM - 12:30 PM

Influence of BMI on Aerobic Fitness and Academic Performance in Middle School Students

Guoyuan Huang1, Xiangrong Shi, FACSM2, Audra Offutt3. 1University of Southern Indiana, Evansville, IN. 2University of North Texas Health Science Center,, Fort Worth, TX. 3Thompson Middle School of EVSC, Evansville, IN.

(No relationships reported)

PURPOSE: Obesity is one of the most pressing health concerns for children. Could positive results presenting an academic advantage be due to children more fit in BMI? The current study was to explore if middle school students who are BMI fit are likely to have better aerobic fitness and stronger academic performance.

METHODS: The study sample included 146 middle school students (mean age: 11.9±0.4 yrs). Subjects were administered physical fitness tests after first signing the informed consent. The study used standard physical fitness tests and was conducted in a controlled manner by the trained professionals. The academic performance information was collected, including previous and current semester GPA (P-GPA; C-GPA), and final GPA for the academic year (F-GPA). Only those students with complete sets of scores for both variables were included in the statistic analysis. SPSS for Windows 17.0 was used for statistical analysis.

RESULTS: The results showed a significant mean difference of aerobic fitness in terms of 1-mile running time (min) between normal-BMI and overweight-obese-BMI students (mean±SD, 9.99±2.67 vs. 13.51±2.61; p<0.0001), and between underweight-BMI and overweight-obese-BMI students (10.38±2.52 vs. 13.51±2.61; p<0.0001). Students with normal-BMI tended having better academic performance than that of the overweight-obese-BMI students in terms of percentile rank for F-GPA (58.97±26.47 vs. 46.27±28.01; p=0.022) and C-GPA (59.02±26.05 vs. 47.56±26.91; p=0.041). Better GPAs were also found in normal-BMI students, compared to the underweight-BMI, in P-GPA (58.25±26.13 vs. 41.43±24.16; p=0.009), C-GPA (59.02±26.05 vs. 38.13±26.67; p=0.001), and F-GPA (58.97±26.47 vs. 40.50±26.09; p=0.004).

CONCLUSIONS: This study suggests that normal-BMI students are likely to have high level of cardiorespiratory fitness, compared to those students with underweight-BMI and overweight-obese-BMI. Students with healthy BMI tend to have higher GPAs, showing better academic performance.

1291 Board #72 MAY 30 11:00 AM - 12:30 PM

Relationship Of Exercise Class Participation and BMI Change In Pediatric Obesity Patients

Christopher Kist, Amanda Gier, Wayne Mays, Bob Siegel, Shelley Kirk. Cincinnati Children’s Hospital, Cincinnati, OH.

(No relationships reported)

PURPOSE: To evaluate the relationship of participation in a pediatric weight management program inclusive of exercise classes and family-oriented dietary counseling to body mass index (BMI) change pre and post participation.

METHODS: We evaluated 269 pediatric obesity patients (mean age 11.2 ± 3.0 years, 106 males) with a 400 meter walk test pre and post participation in the pediatric weight management program. There were 178 patients that participated in exercise classes (EC) and 91 patients that completed the program without participation in the exercise classes (NC). Heart rate difference (HRDiff) was calculate by subtracting the pre 400 meter walk heart rate (HR) from the post 400 meter walk HR.

RESULTS: For the total group, there was a significant correlation between the change in BMI (0.12 ± 2.13 kg) and the percentage of exercise classes attended (12.3 ± 16.2%, r=0.21, p<0.0005). Additionally, the initial BMI (34.7 ± 7.7 kg/m2) correlated with the pre and post HR Diff (30 ±13 and 29 ± 13 BPM) (r=0.15, p<0.01 and r=0.16, p<0.01) Also, the post BMI was significantly correlated with the post HRDiff (r=0.14, p<0.05). The NC group showed no correlations with pre or post HRDiff. However, the EC group showed significant correlation between the change in BMI (0.43 ± 2.46 kg) and the percentage of exercise classes attended (18.6 ± 16.7%, r=0.25, p<0.0005). Additionally, the initial BMI (34.8 ± 7.9 kg/m2) correlated with the pre and post HR Diff (29 ±13 and 28 ±13 BPM) (r=0.20, p<0.005 and r=0.20, p<0.005) Also, the post BMI was significantly correlated with the post HRDiff (r=0.17, p<0.005). DISCUSSION: There was a significant correlation between the change in BMI pre and post exercise participation and the percentage of classes attended. This relationship was strengthened when patients that did not attend classes were removed from the analysis. Additionally, BMI was significantly correlated with HR difference from the 400 meter walk test indicating BMI relationship to the difficulty of the walk test.

CONCLUSION: There was a significant but weak correlation of BMI change to attendance to exercise classes in a pediatric weight management program. Further multivariate analysis may illuminate the relationship between attendance and BMI.

1292 Board #73 MAY 30 11:00 AM - 12:30 PM

Influence Of BMI On The New FITNESSGRAM® Aerobic Capacity Criteria Standards In Sixth-grade Children.

Tinker D. Murray, FACSM1, John Walker, FACSM1, Pete Silvius2, Erik Silvius2, James Eldridge3, William G. Squires, Jr., FACSM4. 1Texas State University, San Marcos, TX. 2Seguin ISD, Seguin, TX. 3University of Texas of the Permian Basin, Odessa, TX. 4Texas Lutheran University, Seguin, TX.

(No relationships reported)

New criterion standards for percent body fat, body mass index (BMI), and aerobic capacity have been developed for the FITNESSGRAM®. Aerobic capacity is calculated based on 1-mile run, PACER, or 1-mile walk performances, as well as BMI values. Criterion standards for aerobic capacity include a healthy fitness zone (HFZ) and a categories for Needs Improvement - Some Risk (NI-SR) and Needs Improvement - High Risk (NI-HR).

PURPOSE: The purpose of this study was to examine the influence BMI has on the new FITNESSGRAM® criterion standards for aerobic capacity compared to the previous standards for one-mile run and PACER tests in sixth-grade children.

METHODS: Subjects were 439 sixth-grade boys and girls (61.3% Hispanic; 7.1% African-American) who completed each of the FITNESSGRAM® test components of their yearly physical education assessment. While, 60% of students met the previous FITNESSGRAM® age and gender criterion standards for BMI, only 43% met the new HFZ standards, and only 2% were in the very lean category. For the remaining students, 13% were classified as NI-SR, while 42% were classified as NI-HR.

RESULTS: The percent of these students meeting the previous criterion standards were 48% for the one-mile run, and 44% for the PACER test. The percent of these students meeting the new FITNESSGRAM® age and gender aerobic capacity standards calculated from one-mile run times were 55% for the HFZ, 19% for NI-SR, leaving 26% in the NI-HR category. The percent meeting the new aerobic capacity standards calculated from PACER performance were 50% for the HFZ, 19% for NI-SR, leaving 31% in the NI-HR category. Logistic regression revealed that compared to students who failed to meet the new HFZ standard for BMI, students who met the new HFZ standards for BMI were 42.6 times more likely to meet the aerobic capacity HFZ standard determined from one-mile times, and 57.1 times more likely to meet the aerobic capacity HFZ standard determined by PACER performance.

CONCLUSIONS: A decrease in BMI of one unit (based on weight loss only, ∼2.38 kilograms) would increase the probability of meeting HFZ criterion standards for aerobic capacity determined from the one-mile run by 45% and for aerobic capacity determined from PACER performance by 71%. This achievable (and safe) goal in similar youth populations remains to be tested in fitness interventions.

1293 Board #74 MAY 30 11:00 AM - 12:30 PM

The Effect of Waist Circumference on FITNESSGRAM® BMI and Aerobic Capacity Criterion Standards in Sixth-Grade Children.

John L. Walker, FACSM1, Tinker D. Murray, FACSM1, James Eldridge2, Pete Silvius3, Erik Silvius3, William G. Squires, Jr., FACSM4. 1Texas State University - San Marcos, San Marcos, TX. 2University of the Permian Basin, Odessa, TX. 3Seguin Independent School District, Seguin, TX. 4Texas Lutheran University, Seguin, TX.

(No relationships reported)

Waist circumference has been identified as an indicator of metabolic syndrome. New FITNESSGRAM® health fitness zone (HFZ) standards for body composition and body mass index (BMI) have been established to identify students most at risk for metabolic syndrome. New HFZ standards have also been established for aerobic capacity (AC).

PURPOSE: The purpose of this study was to determine the effect of waist circumference (WC) on the new FITNESSGRAM® BMI and AC HFZ standards in sixth-grade children.

METHODS: Subjects were 528 sixth-grade boys and girls who completed each of the FITNESSGRAM® test components as a part of their yearly physical education assessment. AC was determined from one-mile run times. In addition to height and weight, subjects were also measured for WC. The percent of these students meeting the FITNESSGRAM® age and gender HFZ standards were 49% for BMI, and 59% for AC, compared to passing rates of 60% for BMI, and 35% for the one-mile run based on the previous FITNESSGRAM® standards.

RESULTS: The correlation between BMI and AC was .74. The correlation between WC and AC was .71. The correlation between BMI and WC was .94. Multiple regression indicated that BMI and gender accounted for 62% of the variation in AC. WC and gender accounted for 61% of the variation in AC. Logistic regression revealed that students who met the HFZ standards for BMI were 76.7 times more likely to meet the HFZ standards for AC than students who failed to meet the HFZ standards for BMI. A decrease in BMI by one unit would increase the probability of meeting the HFZ standards for AC by 41%. A decrease in WC by one inch would increase the probability of meeting the HFZ standards for AC by 37%.

CONCLUSIONS: The influence of BMI on AC is similar to that of WC. Receiver Operating Characteristic (ROC) analysis indicated that a waist circumference of 29 inches is the optimal cut-off score for determining successful completion for either BMI (AUC = .97) or AC (AUC = .93). These data suggest that interventions targeting a reduction in waist circumference or weight loss have the potential to improve performance on health-related fitness tests of aerobic capacity. Recent reports indicating an increase in overweight and obesity among children and adolescents make the development of such interventions even more meaningful for appropriate health-related fitness in youth.

1294 Board #75 MAY 30 11:00 AM - 12:30 PM

School Physical Education Programs And Children’s Cardiovascular Fitness Tracking

Randall A. Nichols, Traci D. Zillifro, Wenhao Liu. Slippery Rock University, Slippery Rock, PA.

(No relationships reported)

PURPOSE: To compare cardiovascular fitness tracking between children from two different PE programs.

METHODS: Participants were 176 children (85 girls) in a PE4life Academy middle school (S1), which was known for its high level of moderate and vigorous physical activity (MVPA) in the PE class, and 118 children (63 girls) in a same-area middle school with a traditional PE program (S2). Two schools were similar in socioeconomic status, race composition (white > 93%), and weekly PE class time (approximately 90 minutes per week). Progressive Aerobic Cardiovascular Endurance Run (PACER) was administered to the cohort at the beginning and end of their middle school period. Spearman rank correlation was used to examine stability of cardiovascular fitness. Further, based on the criteria of FITnessgram Healthy Fitness Zone (HFZ), the cohort was categorized into either an at-risk group or HFZ group, and Chi-square test was used to examine differences in distribution changes in at risk and HFZ between S1 and S2 across the three years. Children’s MVPA in the PE class were coded for 25 lessons in each school with System for Observing Fitness Instruction Time and analyzed with one-way MANOVA.

RESULTS: PACER correlations were .64 for S1 and .73 for S2 for boys, and were .77 for S1 and .49 for S2 for girls. Chi-square results for boys indicated similar distribution pattern in the two groups between S1 (49.1% in the at-risk group and 50.9% in the HFZ) and S2 (57.1% and 42.9%) at baseline. At the follow-up, however, the patterns became different between S1 (24.5% in the at-risk group and 75.5% in the HFZ) and S2 (81.0% and 19.0%), with χ2 = 19.86 and p < .001 in favor of S1. Results for girls were similar. At baseline S1 had 21.6% in the at-risk group and 78.4% in the HFZ, and S2 had 33.3% and 66.7%, respectively. At the follow-up S1 had 29.4% in the at-risk group and 70.6% in the HFZ, and S2 had 61.5% and 38.5%, respectively, with χ2 = 9.23 and p < .01 in favor of S1. Further, MANOVA results indicated that children in S1 had significantly larger percentage of PE class time in MVPA than those in S2 (p < .001), with 66.42 ± 6.50 vs. 44.12 ± 8.79.

CONCLUSIONS: The PE4life program has much more positive tracking results in children’s cardiovascular fitness than traditional PE programs. But Spearman rank correlation alone could not identify or explain the differences.

1295 Board #76 MAY 30 11:00 AM - 12:30 PM

Physical Activity Incorporated Within Head Start Curriculum Improves Early Literacy In African American Children

Stacie M. Kirk, Erik P. Kirk. Southern Illinois University Edwardsville, Edwardsville, IL.

(No relationships reported)

PURPOSE: To determine the effects of a low cost, teacher directed, minimal intervention program that delivered existing academic lessons taught through physical activity (PA) in Head Start preschools serving African American (AA) children.

METHODS: Seventy-two African American children (mean±SE, age 3.8±0.1y) from a low socioeconomic urban Head Start were randomized to Control (CON, n=3 classrooms, n=21, F, n=9 F, M, n=12) or PA (n=4 classrooms, n=51 F, n=29, M, n=22) groups for 6 mo. The PA program was designed to promote 150 min/wk of moderate to vigorous PA academic lessons (3.0-6.0 METS, ∼15 min each, 2 lessons/day). Intensity of classroom PA was measured by a SOFIT. Height and weight to determine BMI were obtained using a stadiometer and digital scale. The Get It, Got It, Go Individual Growth and Development Indicators for children ages 3-5y was administered to assess early literacy and phonological awareness in the areas of alliteration and picture naming. All measures were assessed at baseline, 3 and 6 mo.

RESULTS: The PA protocol resulted in significantly (p<0.05) greater levels of PA in the classroom during free play at 3 (PA, 8.1±2.1% vs. CON, -2.2±2.1%) and 6 (PA, 20.1±2.1% vs. C, -7.1±2.1%) mo than children in the CON, resulting in between group differences at 3 and 6 mo, p<0.01. The 6 mo change in BMI for the PA group was 2.0±1.1% and CON 0.8±0.9%, respectively (NS). Alliteration (letters sounds/two min) significantly (p<0.01) improved in the PA group from baseline (0.4±0.1) to 3 mo (1.0±0.4) and was maintained at 6 mo (1.0±0.4) compared to no change in CON at 3 (0.1±0.3) and 6 (0.2±0.4) mo compared to baseline, p>0.05, resulting in between group differences at 3 and 6 mo, p<0.01. Picture naming (picture per minute) significantly (p<0.01) improved in the PA group from baseline (17.2±3.5) to 3 (21.7±5.0) and 6 (24.8±5.4) mo compared to no improvement in the CON, resulting in between group differences at 3 and 6 mo, p<0.01. Higher scores for alliteration and picture naming indicate greater early literacy skills and phonological awareness.

CONCLUSION: A low cost, teacher directed, minimal intervention program incorporating PA within Head Start curriculum promotes daily PA, increases early literacy, and attenuate increases in BMI in AA preschool children, a group at an increased risk for poor literacy development and obesity.

1296 Board #77 MAY 30 11:00 AM - 12:30 PM

Effect of Exercise Training in the Cold on Regional Body Composition in Obese Boys

Boguslaw Wilk, FACSM, Joyce Obeid, Raymond Trott, Oded Bar-Or, Brian W. Timmons. McMaster University, Hamilton, ON, Canada.

(No relationships reported)

Exercise training in the cold reduces whole body fat mass in obese adolescent boys (Wilk et al 2006).

PURPOSE: To determine the effect of exercise training in the cold on regional body composition, substrate utilization, respiratory heat loss (RHL), and energy expenditure (EE) in obese adolescents.

METHODS: Twenty obese (>30% body fat) adolescent boys, ages 12 to 16 years, participated in a 6-week (3 times per week) exercise training program. Boys were randomly allocated to train in the cold (COLD; 5 °C, n=10) or a thermoneutral environment (NEUT; 24.5 °C, n=10). Each training session consisted of 3 × 20 min exercise bouts interspersed with 5 min rest performed in a climate chamber. The boys alternated between treadmill walking and cycling (both at 60 % VO2max) within each session. Regional body composition was measured before and after the 6-week training program using DXA. Volume, O2, CO2, and temperature of expired air were measured during exercise at the first (T1) and last (T18) training sessions to assess EE, substrate utilization (fat oxidation (FATox) and carbohydrate oxidation (CHOox)), and RHL. Two-way ANOVAs with between (COLD vs. NEUT) and within (pre- vs. post-training) factors were used (p<0.05).

RESULTS: Significant post-training %fat changes were observed for trunk (-1.6 %, P= 0.05) and legs (-1.0%, P=0.001) in COLD and for arms in NEUT (-1.2%, P=0.04). Leg lean mass increased 4.6% (P=0.001) in COLD only. FATox was higher and CHOox lower at T18 compared with T1 (P<0.05), with no differences between groups. RHL were higher (P=0.001) in COLD at T1 (1.08 kcal/kg) and T18 (1.04 kcal/kg) compared with NEUT (0.61 and 0.57 kcal/kg at T1 and T18, respectively). Although EE was ∼10% higher in COLD compared with NEUT at both T1 and T18, this difference was not statistically significant.

CONCLUSIONS: Six weeks of exercise training in the cold induces desirable changes in regional body composition, particularly central adiposity, of obese boys.

1297 Board #78 MAY 30 11:00 AM - 12:30 PM

Examining Elementary School Students’ Motor Skills, Physical Activity, and Health-related Physical Fitness

Tao Zhang1, Ping Xiang2. 1University of North Texas, Denton, TX. 2Texas A & M University, College Station, TX.

(No relationships reported)

Promoting motor skills, physical activity (PA), and health-related physical fitness are all critical to our effort to help school-aged students live physically active lifestyles. Therefore, understanding their interrelationships is considered a research priority. While considerable research has examined PA in relation to students’ health-related physical fitness, research on the relationships among students’ motor skills, in-class PA and physical fitness has been scarce.

PURPOSE: The purpose of this study was to investigate the relationships among motor skills, in-class PA, and health-related physical fitness among elementary school students in physical education (PE).

METHODS: Participants were 104 fourth and fifth grade students (45 girls and 59 boys; M age = 11.0). They completed motor skills in basketball, overhand throwing, and striking assessed by PE Metrics TM (NASPE, 2010). Students’ PA across three PE lessons was measured by Actical accelerometers (Mini-Mitter Co., Inc., Bend, OR). Health-related physical fitness was assessed by FITNESSGRAM® (Cooper Institute, 2007), including Progressive Aerobic Cardiovascular Endurance Run, curl-ups, push-ups, height and weight (calculating BMI), trunk lift, and sit-and-reach tests. The total number of fitness tests in which students achieved the Healthy Fitness Zone was calculated and used as an overall measure of physical fitness.

RESULTS: There were positive relationships among basketball, overhand throwing, and striking. Basketball was positively associated with in-class PA. Health-related physical fitness was positively correlated with basketball, striking, and in-class PA. Two regression analyses indicated basketball and overhand throwing were significant predictors of students’ in-class PA (R2 = 6.3 %; β = .22, -.24, respectively, all p < 0.05). The basketball skill emerged as a significant predictor of students’ physical fitness (R2= 4.7 %; β = .22, p < 0.05).

CONCLUSION: These results provided empirical evidence on relationships among students’ motor skills, PA, and health-related physical fitness. Given the small predictive strength of motor skills in this study, additional investigation is needed to further examine the dynamic relationships among motor skills, PA, and health-related physical fitness.

A-31 Free Communication/Poster - Clinical Exercise Physiology Cardiovascular (Clinical Exercise Physiology Association)

MAY 30, 2012 7:30 AM - 12:30 PM

ROOM: Exhibit Hall

1298 Board #79 MAY 30 9:30 AM - 11:00 AM

Blind Reading Of Anaerobic Threshold (AT) To Detect Changes In Exercise Tolerance

Kazuo Kondou1, Hirotaka Nishijima1, Namiko Horigome1, Oukusa Takanori1, Kazuya Yonezawa2, Masayuki Sakurai1. 1Hokko Memorial Hospital, Sapporo, Japan. 2National Hospital Organization Hakodate Hospital, Hakodate, Japan.

(No relationships reported)

PURPOSE: Although AT is a useful measure of exercise tolerance (ET), it has a drawback of requiring an evaluator, a source of subjectivity. This study was done to assess the AT by blind reading to detect changes in ET.

METHODS: The records of 166 patients who underwent cardiopulmonary exercise testing (CPX) during cardiac rehabilitation (CR) were retrospectively reviewed. CR was hospital (the mean of 3.1/mo) and home based. The mean time elapsed between the 1st and 2nd tests was 135(SD:29) days. The mean age was 64(11), with 143 males and 23 females. The underlying heart disease was mainly ischemic: post myocardial infarction in 95 and angina in 39. CPX was done using an upright bicycle with a breath-by-breath apparatus. The exercise was submaximal, which was mostly terminated with an appearance of AT or target heart rate. CPX records were erased of their ID’s and coded by the controller. AT was read by 2 independent experienced investigators(A,B) based on the Wasserman’s criteria.

RESULTS: The table below shows the mean(SD).

Table

Table

The investigator A did not detect AT in 21.7% and B in 14.5%. About 2/3 of these cases had a R<1.0 at the highest workload, suggesting a workload below AT. Although the pre-and post-CR AT values read by the investigators A and B were clearly different (p<0.001), the mean pre-post difference in AT detected by each was remarkably similar (51(161) vs 46(140) ml/min in VO2, ns). We speculated that since the entire portion of the v-slope would shift to the right with training, any identifiable deflection point on it would equally shift in parallel

CONCLUSIONS: Despite differing AT readings at pre-and post-rehabilitation by the 2 independent investigators, the mean absolute difference (improvement) did not differ between the 2.

1299 Board #80 MAY 30 9:30 AM - 11:00 AM

Effect Of An Exercise-based Cardiac Rehabilitation Program On Arterial Stiffness In Post Myocardial Infarction Patients

Norton Luis Oliveira1, Fernando Ribeiro2, Alberto Jorge Alves1, Fátima Miranda3, Marlene Fonseca3, Lilibeth Campos3, Madalena Teixeira3, José Oliveira1. 1University of Porto, Faculty of Sport, Porto, Portugal. 2CESPU, Polytechnic Health Institute of the North, Gandra PRD, Portugal. 3Centro Hospitalar de Gaia/Espinho, Vila Nova de Gaia, Portugal.

(No relationships reported)

The arterial stiffness, assessed by pulse wave velocity (PWV), has been showed to be an independent cardiovascular risk marker in several clinical conditions. Exercise seems to be an effective intervention in decreasing PWV in several chronic diseases, although few studies exist in post myocardial infarction (MI) patients

PURPOSE: To determine the effect of an exercise-based cardiac rehabilitation program on PWV in post MI patients.

METHODS: Twenty-four male and 2 female patients, mean (±SD) age 60.1 ± 10.5 years, after an acute myocardial infarction were randomized to either an 8-week exercise-based cardiac rehabilitation program (EG) or to a control group, which underwent only usual medical care (CG). Complete randomization was performed by choosing one of two sealed envelopes. The EG participated in an 8-week programme comprising 3 exercise sessions per week. Each exercise session included 10 minutes of warm-up, 35 minutes of aerobic exercise on a cycloergometer or treadmill, and 10 minutes of cool-down. The exercise intensity was calculated as 65-75% of maximal heart rate achieved in the exercise test. Outcome measures included changes in PWV, evaluated by applanation tonometry, and peak oxygen consumption (VO2). Group comparisons were made using two-way ANOVA with repeated measures.

RESULTS: Age (EG - 63 ± 9.4 vs. CG - 57 ± 11.2 years), PWV (EG - 9.2 ± 2.3 vs. CG - 8.2 ± 2.5 m/s) and VO2 (EG - 26.4 ± 6.6 vs. CG - 28.2 ± 5.4 ml/kg/min) were not significant different at baseline (p>0.05). Moreover, patients did not change their medication in the study period. The main effect of cardiac rehabilitation for PWV was significant (F 1.24 = 5.4) indicating a decrease in EG (8.2 ± 2.0 m/s, p0.05). The main effect was also significant for VO2 (F 1.24 = 6.6) with an average increase of 3.4 ml/kg/min in EG (29.8 ± 8.1 ml/kg/min, p<0.05) and no significant change in CG (28.5 ± 6.1 ml/kg/min).

CONCLUSIONS: Exercise-based cardiac rehabilitation decreases systemic arterial stiffness in post MI patients. Therefore, this could be one of the mechanisms associated with the positive outcome (i.e., mortality rates) on post MI patients who undergo rehabilitation.

ClinicalTrials.gov Identifier: NCT01432639

Supported by the following grants from The Portuguese Fundação para a Ciência e a Tecnologia (FCT): SFRH/BD/48875/2008 and PTDC/DES/113753/2009

1300 Board #81 MAY 30 9:30 AM - 11:00 AM

Double Product Based on Heart Sounds as a Marker of Catecholamines During Exercise

Takuro Matsuda1, Yasuki Higaki1, Akira Kiyonaga1, Shigeru Obara2, Hiroaki Tanaka1. 1Fukuoka University, Fukuoka, Japan. 2The University of Tokushima, Tokushima, Japan.

(No relationships reported)

PURPOSE: It is well documented that exercise-induced adrenergic stimulation enhances myocardial contractility in healthy humans. The break-point of the double product of heart rate (HR) and systolic blood pressure, which is a marker of cardiac oxygen demands, is strongly correlated with the epinephrine (Ep) threshold. The amplitude of the first sound (HS1) is a marker of cardiac contractility, which is a major determinant of cardiac oxygen demand and is easy to determine continuously during exercise. This study investigated the relationship between the double product (DP) based on heart sounds and Ep level.

METHODS: Twelve subjects, with a mean±SD age of 22±3 years and a mean±SD BMI of 23.1±3.5 kg/m2, underwent graded cycle ergometer testing. The work rate started at 15 watts and then increased at 15 watts every 2 min until the heart rate reached 85% of its maximum expected for their age. The heart sounds were collected in a personal computer via an A/D converter throughout the exercise test. Blood from the antecubital vein was sampled and Ep levels were measured every 2 min during the test. Values of the DP were calculated by the formula HR×HS1. The break-point of DP (DPBP) and Ep threshold were determined based on a visual inspection three separate times and the mean value was calculated.

RESULTS: The DP was significantly correlated with Ep levels in all subjects during incremental exercise (r=0.97 to 0.99, p<0.001). A clear DPBP and Ep threshold were obtained in all subjects (DPBP: 84.6±13.9 watts; Ep threshold: 83.5 ±19.5 watts) and they were not statistically significant form each other (p=0.877).

CONCLUSIONS: The DP from heart sounds is a valid marker of the sympathetic nerve system during exercise stress. Monitoring heart sounds appears to provide valuable information related to the level of exercise stress. This information may be useful when determining an appropriate exercise prescription.

This work was supported by the Ministry of Education, Culture, Sports, Science, and Technology, Japan (No. 22300240 and Strategic Research Infrastructure No. 0801083), a grant from GFP at Fukuoka University.

1301 Board #82 MAY 30 9:30 AM - 11:00 AM

Is Cardiorespiratory Fitness, Muscular Fitness or Physical Activity Related to Diabetes Risk in Postmenopausal Women?

Judith A. Flohr, Elizabeth S. Edwards, Layne E. Eidemiller, Christine M. Nicewonger, Christopher J. Womack, FACSM. James Madison University, Harrisonburg, VA.

(No relationships reported)

PURPOSE: The purpose of this study was to examine the independent and joint associations of upper and lower body muscular strength (MS), cardiorespiratory fitness (CRF), and physical activity (PA) with the prevalence of Type 2 diabetes mellitus (T2DM) in a group of sedentary healthy postmenopausal women.

METHODS: Thirty-five postmenopausal (age 57.9 ± 4.7 yrs.) women completed a graded exercise test on a treadmill to determine maximum relative oxygen consumption (VO2max) and the International Physical Activity Questionnaire (IPAQ) to estimate MET minutes/week of PA. Waist circumference (WC), body fat via DXA (BF), height, weight, 5RM leg press and chest press were measured and use to estimate 1RM. These 1RM values were then divided by body weight, yielding ratios for leg press (LP) and chest press (CP). All participants had blood drawn and analyzed for HbA1c (A1c). Bivariate Pearson’s correlations were used to identify variables that were associated with A1c. Stepwise regression was then used to identify variables that significantly predicted A1c.

RESULTS: The average A1c was 5.9%. Both LP (r = -0.344, p = .043) and CP (r = -0.414, p=.013) were significantly associated with A1c, but neither VO2max nor IPAQ were significantly correlated with A1c. In the regression model, only LP significantly predicted A1c, b = -0.57, t (33) = -2.61, p = .013. LP explained a significant proportion of the variance in A1c, R2 = 0.17, F (1, 33) = 6.84, p = .013.

CONCLUSIONS: Previous studies have reported an inverse relationship among CRF, PA, MS and T2DM in men. There is limited date available describing the relationship between MS and A1c in postmenopausal women. However, recent reports suggest that muscle mass may play a significant role in glycemic control. The results of the present study indicate that muscular strength as measured by LP and CP were related to A1c. Therefore, efforts to increase muscle strength in postmenopausal women in an attempt to lower diabetes risk appear to be warranted.

1302 Board #83 MAY 30 9:30 AM - 11:00 AM

The Effects Of Aerobic Training In The Patients With Heart Failure

Shang-Lin Chiang, Chinese. Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan. (Sponsor: Wen-Hsu Sung, FACSM)

(No relationships reported)

PURPOSE: Aerobic training can improve not only aerobic capacity but also symptoms and survival rate in patients with heart failure (CHF). The purposes of our study will test the effects of exercise training on aerobic capacity, heart rate, blood pressure, cadioutput, body composition, serum lipid and NT-proBNP (aminoterminal fragment brain natriuretic peptide prohormone) in CHF patients, and examine the potential benefit of changes over time.

METHODS: The samples consisted of 13 eligible cases with heart failure. Seven of them completed 12-week exercise program ( 30 minutes a day, 3 days a week, at 60 % peak oxygen uptake (VO2peak)). Every 4 weeks training, heart rate, blood pressure, body composition, serum lipid and NT-proBNP were measured after an overnight fast. Besides, VO2peak and cadioutput were measured at pre-training and post-training.

RESULTS: After 12-week exercise training program, seven cases completed the process. The results indicate that the parameters of cardiopulmonary capacity were all significant improved. The VO2peak was improved from 20.30±5.30 to 22.49±4.36 ml/kg/min (p=.002). The maximal metabolic equivalent (MET) was improved from 5.76±1.55 to 6.37±1.31 METs (p=.0001). The maximal work load was improved from 220.43±76.39 to 276.57.43±75.91Watt (W)(p=.029) Although it was not significant, diastolic pressure and systolic pressure were improved over training time. Meanwhile, after 12-week training, static cardioutput was not significant changed, but dynamic cardioutput was significant improved (increased 2.37ml/min p=.015). However, total cholesterol and NT-proBNP did not decreased significantly. But the more time of exercise training they received, the more they improved.

Conclusion: It is concluded that aerobic exercise training could improve cardiopulmonary capacity, static heart rate, blood pressure, cardioutput, body composition, serum lipid and NT-proBNP in heart failure patients. The results also proved the effects of aerobic exercise for heart failure patients could be accumulated over time. Therefore, provide precise exercise prescriptions and educate them how to do the adequate aerobic exercise training are very important to patients with hear failure.

1303 Board #84 MAY 30 9:30 AM - 11:00 AM

Heart Rate Variability is Reduced at Peak Exercise in Individuals at Risk for Sleep Apnea

Trent A. Hargens1, Amanda Mallory2, Steve Vesbach2. 1James Madison University, Harrisonburg, VA. 2Ball State University, Muncie, IN. (Sponsor: M. Kent Todd, FACSM)

(No relationships reported)

Obstructive sleep apnea (OSA) is a sleep disorder that significantly increases risk for hypertension, cardiovascular disease (CVD) and diabetes, among others. OSA leads to dysfunction in the autonomic regulation of the cardiovascular system, a primary mediating factor in the increased risk for chronic disease. Heart rate variability (HRV) has been widely used for assessing autonomic function, and has been shown to be predictive of CVD and all-cause mortality at rest and during exercise. HRV has been shown to be reduced in OSA at rest, but to date, no studies have examined exercise HRV in individuals diagnosed with, or at high risk for, OSA.

PURPOSE: To examine the HRV responses during exercise in individuals identified as high risk for OSA.

METHODS: Nine (6 male, 3 female) obese subjects (BMI: Mean SD = 33.3 6.4 kg/m2; age = 47.4 8.2 yr) at risk for OSA (OSA) and 16 (6 male, 9 female) obese subjects (BMI = 32.5 4.9; age = 28.7 10.3) at low risk for OSA (Control) performed maximal cycle ergometer exercise tests utilizing a 20 watts/min ramp protocol. HRV was assessed at rest, during exercise and immediately post-exercise. Measures of time and frequency domain HRV were obtained. HRV variables were log transformed and converted into normalized units for statistical analysis. Risk for OSA was established through a screening result from an at-home sleep assessment device (Embletta).

RESULTS: The OSA group was significantly older than the control group (P < 0.001). Body mass index, blood pressure, resting heart rate, and VO2peak did not differ between groups. After controlling for age and gender, peak exercise (last two minutes of exercise) log total power (0.74 0.2 vs. 1.5 0.2 ms2), log low frequency (-0.47 0.3 vs. 0.31 0.2), log high frequency (-0.92 0.3 vs. 0.27 0.2), normalized low frequency (1.03 0.5 vs. 3.03 0.4), and normalized high-frequency (0.60 0.5 vs. 3.00 0.4) were reduced in the OSA group (P < 0.05). No differences were noted in time domain variables at peak exercise. Resting, submaximal exercise or recovery HRV variables (time or frequency domain) did not differ.

CONCLUSION: Results suggest that individuals at high risk for probable OSA display abnormal autonomic modulation of heart rate during peak exercise. Exercise HRV may be a useful tool for improving risk assessment in OSA, but needs further examination.

1304 Board #85 MAY 30 9:30 AM - 11:00 AM

Early Oxygen Uptake Response to Constant Work Exercise and Mortality in Men

Kai P. Savonen1, Vesa Kiviniemi2, Heikki Rajala1, Jari A. Laukkanen1, Timo A. Lakka1, Rainer Rauramaa, FACSM1. 1Kuopio Research Institute of Exercise Medicine, Kuopio, Finland. 2Information Technology Center, University of Eastern Finland, Kuopio Campus, Kuopio, Finland.

(No relationships reported)

The blunted pulmonary oxygen uptake (VO2) response to constant work exercise is associated with a poor prognosis in congestive heart failure patients but no previous study has addressed this issue in asymptomatic subjects.

PURPOSE: To study the association between a blunted VO2 increase during the first 30 s of the constant work exercise in cycle ergometer and the risk of cardiovascular death in cardiovascularly healthy middle-aged men.

METHODS: The subjects were 484 men, 54-56 years of age, from eastern Finland without cardiovascular disease or cancer at baseline. VO2 response to constant work exercise at 50 Watts in cycle ergometer was quantified by calculating the average net VO2 during the first 30 s of exercise (VO230) in proportion to the average net VO2 at steady state (VO2ss, the average net VO2 during the third minute of exercise). The association of VO2 response with the risk of cardiovascular death was examined with multivariable Cox proportional hazards’ regression model. A total of 52 cardiovascular deaths were observed during an average follow-up of 19.8 years.

RESULTS: The average net VO2 during the first 30 s of exercise in proportion to the average net VO2 at steady state was not related to the risk of cardiovascular death. In Cox-multivariable model, the risk of death increased 8% (95% CI -19-43, p=0.58) for a 1-SD (0.19) increment in VO230/VO2ss after adjustment for myocardial ischaemia during exercise test, cigarette smoking, systolic blood pressure at rest, and body mass index.

CONCLUSION: An attenuated early VO2 response during the first 30 s of constant work exercise does not predict cardiovascular death in cardiovascularly healthy middle-aged men. While submaximal testing itself offers certain advantages, the early VO2 response can not be utilized for prognostic assessment in healthy men.

1305 Board #86 MAY 30 9:30 AM - 11:00 AM

Blood Flow Restriction Increases Angiogenic Gene Expression in Skeletal Muscle following Acute Resistance Exercise

Kelly A. Larkin, Richard G. MacNeil, Launa Clough, Bhanupresad Sandesara, Todd M. Manini, Thomas W. Buford. University of Florida, Gainesville, FL.

(No relationships reported)

Blood flow restriction (BFR) potentiates the effects of low-intensity resistance exercise on skeletal muscle size and strength. However, evidence is sparse regarding the effects of BFR exercise on vascular adaptations within the skeletal muscle.

PURPOSE: To evaluate the effects of BFR on post-exercise expression of angiogenesis-related mRNAs.

METHODS: Using a randomized cross-over design, six healthy young adults (n=3 men, n= 3 women, 22±1 years) performed on separate occasions low-intensity exercise with and without BFR. Each exercise bout entailed 120 repetitions of single-limb knee extensor exercise at 40% of 1RM maximum. The BFR condition was performed with an external pressure cuff inflated to 220 mm Hg. Near-infrared spectroscopy (NIRS) was utilized to measure oxygenation of the vastus lateralis during exercise. Skeletal muscle samples were collected via percutaneous biopsy prior to, 4 hours following, and 24 hours following exercise. Skeletal muscle mRNA expression was determined using standard Q-PCR techniques and normalized to GADPH. NIRS data were analyzed using paired Student’s t-tests and Q-PCR data were analyzed using the Wilcoxon’s test.

RESULTS: BFR (45.3±2.4%) decreased tissue oxygenation during the exercise bout compared to control exercise (59.7±2.9%, p=0.028). This was reflected in a significant increase in total Hb (14.4±2 vs. 0.9±2, p=0.010) that was driven largely by an increase in deoxygenated Hb. BFR increased (p<0.05) mRNA expression of vascular endothelial growth factor (VEGF), VEGF-R2, hypoxia inducible factor alpha (HIF1α), inducible nitric oxide synthase (NOS), and neuronal NOS. The most notable change in response to BFR was an increase in VEGF mRNA expression at 4 hours post-exercise (4.1±0.6 vs. 0.6±0.2 fold change, p=0.028). Muscle mRNA expression of endothelial NOS was not altered in response to BFR (p>0.05).

CONCLUSIONS: Acute BFR increases post-exercise expression of mRNA related to skeletal muscle angiogenesis.

1306 Board #87 MAY 30 9:30 AM - 11:00 AM

Effect Of Training With Blood Flow Restriction Of The Thigh Muscles In Men

Hans-Christian C. Heitkamp, Beate Schiff, Walter Rapp, Andreas Niess. Univ. of Tuebingen, Tuebingen, Germany.

(No relationships reported)

Training with vascular restriction lately receives more attention since less percentage of 1 RPM seems necessary for gaining strength.

PURPOSE: The aim of this study was to find out whether training with 40 % of 1 RPM leads to more strength with blood flow restriction than without.

METHODS: 14 men aged 27 ± 2.8 years and a BMI of 26 kg/m2 took part in this study. Initially, the strength of the quadriceps and the hamstrings was measured on an isokinetic system isometrically in 60° and 30° positions respectively, isokinetic strength at 60°/s and strength endurance at 120°/s with 25 maximal intensity movements.

Training was conducted for 8 weeks, twice a week for 35 minutes according to the endurance method with 3 sets and 20 repetitions. The right side was always trained under vascular occlusion and both sides at 40 % of 1 RPM. Each leg was trained separately in an alternating mode.

RESULTS: Maximal isometric force of flexion rose from 60 ± 26 to 70 ± 35 Nm (p < 0.001), while it remained constant in the control leg with 58 ± 27 and 57 ± 23 Nm. The extension rose from 82 ± 20 to 94 ± 19 Nm (p<0.001) and dropped in the control leg from 79 ± 21 to 78 ± 24 Nm. During isokinetic flexion, an increase from 106 ± 22 to 124 ± 24 Nm (p<0.01) occurred and in the control leg from 98 ± 22 to 114 ± 24 Nm. During isometric flexion an increase from 198 ± 27 to 219 ± 34 Nm (p < 0.001) and also in the control leg from 201 ± 30 to 210 ± 24 Nm (p < 0.005). The endurance measurement resulted in no increase. The compliance was 83 %.

CONCLUSION: Training with vascular restriction is especially effective for increasing the isometric maximal strength to a lesser degree for the isokinetic maximal strength and without an effect on the endurance of the quadriceps and the hamstrin

1307 Board #88 MAY 30 9:30 AM - 11:00 AM

Heart Rate Index Prediction Of Maximal Oxygen Consumption In Pediatrics During The James Cycle Protocol

Wayne A. Mays, Yvette M. Gerdes, Michelle A. Wolfe, Sandra K. Knecht, Randall P. Claytor, Timothy K. Knilans. Cincinnati Children’s Hospital Medical Center, Cincinnati, OH.

(No relationships reported)

PURPOSE: To evaluate the utility of two versions of heart rate index (HRindex) based on supine and sitting resting heart rates as predictors of maximal oxygen consumption in pediatric patients with structurally normal hearts.

METHODS: We evaluated 637 pediatric patients (mean age 13.8 ± 3.2 years, 354 males) with maximal graded exercise testing utilizing the James cycle ergometer protocol. Two versions of HRindex were calculated by dividing the maximal heart rate by the heart rate (HR) in the resting supine (SupHRindex) and sitting (SitHRindex) positions. Predicted maximal METS (EstMMETS) was generated by the line equation of 6 times the maximal HRindex minus 5 using both the supine HR(SupEstMMETS) and the sitting HR (SitEstMMETS).

RESULTS: Both SupHRindex and SitHRindex were significantly correlated to maximal oxygen consumption (MVO2) (r=0.45 and r=0.43, p<0.0005), maximal indexed oxygen consumption(MIVO2) (r=0.25 and r=0.31, p<0.0005) and maximal metabolic equivalents (MaxMETS) (r=0.25 and r=0.31, p<0.0005). There was a significant correlation between MaxMETS and SupEstMMETS and SitEstMMETS (10.4 ± 2.2 vs 11.1 ± 3.0 r=0.25, p<0.0005 and 10.4 ± 2.2 vs 9.3 ± 2.6 r=0.31, p<0.0005, respectively). However, the absolute values of MaxMETS versus SupEstMMETS and MaxMETS versus SitEstMMETS were significantly different (p<0.0005).

DISCUSSION: There was a significant correlation between HR index calculated using either the supine or sitting resting heart rate and MVO2, MIVO2 and MaxMETS. However, the correlation was weak and provided little predictive value for MVO2, MIVO2 and MaxMETS. This lack of predictive robustness was demonstrated by the significant difference seen in absolute values for MaxMETS compared to both SupEstMMETS and SitEstMMETS.

CONCLUSION: The predictive power of the heart rate index utilizing either the supine or sitting resting heart rate for the calculation is limited in pediatric patients. Future research should focus on a multivariable predictive model that identifies the sources of variability in the correlational relationship of heart rate index and maximal oxygen consumption.

1308 Board #89 MAY 30 9:30 AM - 11:00 AM

Ventilatory Efficiency Is Not Associated With Acsm Heart Disease Risk Factors In Asymptomatic Obese Adults

Michael R. Holly, Bryan L. Smith, Erik P. Kirk, Kevin S. Chapman, Lucas M. Novotny, Ryan D. Beaty, Heather E. Garrett, Jeffrey E. Herrick. Southern Illinois University Edwardsville, Edwardsville, IL.

(No relationships reported)

Graded exercise tests (GXT) are administered to symptomatic heart failure patients as a diagnostic and prognostic assessment of mortality. GXT’s provide an assessment of functional capacity (VO2peak) and ventilatory efficiency (VE/VCO2 slope) and both outcomes are predictive of patient mortality. However, the VE/VCO2 slope has been shown to be a stronger predictor than VO2peak for heart disease severity. The American College of Sports Medicine (ACSM) has established several cardiovascular disease risk factors for determining the risk for cardiac disease in asymptomatic adults.

PURPOSE: This study evaluated the relationship between the VE/VCO2 slope, VO2peak, and the total ACSM heart disease risk factors in asymptomatic obese adults.

METHODS: 29 obese adults free of heart disease (Mean ± SE; Age 46.5 ± 2.6 years; BMI 35.9 ± 1.1 kg/m2) were stratified into low (LR <2 risk factor) or moderate risk (MR ≥2 risk factors) using ACSM’s Logic Model from self reported health history questionnaires. Each subject performed a treadmill GXT at a constant speed of 3.5 mph with 2.5% grade increase every 2 minutes. LR exercised to volitional fatigue and MR until achieving 6 METS, or 76% predicted heart rate maximum. VE/VCO2 slope was determined as the rise in VE (L·min-1 BTPS) to the increase in VCO2 (L·min-1 STPD) throughout the entire exercise session.

RESULTS: There were no significant differences in ventilatory efficiency between risk groups (LR 30.9 ± 0.9, MR 28.6 ± 1.4 VE/VCO2 slope). There were group differences in VO2peak (LR 33.9 ± 3.4, MR 19.7 ± 0.9 ml·kg-1·min-1) and total risk factors (LR 1.0 ± 0.0, MR 3.0 ± 0.25). VE/VCO2 slope was not associated with total risk factors (p = 0.12, r = -0.288) or final risk category (p = 0.17, r = -0.258). VO2peak was associated with total risk factors (p = 0.00, r = -0.614) and final risk category (p = 0.00, r = -0.665). Exercise test and exercise duration were not associated with VE/VCO2 slope.

CONCLUSIONS: Ventilatory efficiency, which has been shown to predict severity of heart disease in symptomatic adults, was not associated with total ACSM heart disease risk factors in this group of asymptomatic obese adults. Our results suggest that VO2peak is inversely related to heart disease risk in asymptomatic obese adults.

1309 Board #90 MAY 30 9:30 AM - 11:00 AM

Classical End-criteria With Impact On Maximal Oxygen Uptake In A Population

Elisabeth Edvardsen, Bjørge Herman Hansen, Sigmund Alfred Anderssen. Norwegian School of Sport Sciences, Oslo, Norway.

(No relationships reported)

During a maximal exercise test, the subjects’ motivation and effort are important requirements to ensure valid and reliable results. Classical end criteria are a plateau in VO2, high RER, HR, [blood lactate] and ratings of perceived exertion. However, there is currently no consensus for the assessment of maximal effort and different end criteria may have an impact of the results.

PURPOSE: to describe classical end criteria during a maximal progressive exercise test in a random sample of 20 - 85 year old population, and to explore if changes in end criteria have an impact on VO2max.

METHODS: A sample of 839 individuals (412 women), with a mean age of 51.0 (SD ± 14.8) performed a progressive exercise test on a treadmill to voluntary exhaustion. Gas exchange, heart rate, [blood lactate] and BORG scale6-20 was measured. A plateau in VO2 was defined by levelling off despite increase in ventilation at maximal power. Different end criteria were used to study the impact on VO2max, such as RER ≥ 1.15 and [blood lactate] ≥ 8 mmol·L-1.

RESULTS: There were no sex related differences in HRmax, RER or BORG scale, while the [blood lactate] was significantly lower in women. When using RER ≥ 1.15 and [blood lactate] ≥ 8.0 mmol·L-1, VO2max increased on average by 4,5 % and 11 %, respectively, compared to using only voluntary exhaustion. Furthermore, a [blood lactate] ≥ 8.0 mmol·L-1 excluded 65 % of the participants in the 50 - 85 year cohort (424 vs 148 respectively).

CONCLUSIONS: A range of typically end criteria are presented in a healthy population. Choice of end criteria during exercise testing will have an impact on VO2max. This shows the importance of maximal effort to ensure valid and reliable results.

Table E

Table E

1310 Board #91 MAY 30 9:30 AM - 11:00 AM

Maximal Strength Training Enhances Functional Performance in Chronic Stroke Patients

Tor Ivar Gjellesvik1, Tessa R. Hill2, Per Marius R. Moen2, Tom Tørhaug2, Marius S. Fimland2, Jan Helgerud2, Jan Hoff2. 1St.Olav University Hospital, Trondheim, Norway. 2Norwegian University of Science and Technology, Trondheim, Norway.

(No relationships reported)

Physical impairment after stroke has been shown to have an immense impact on the reintegration of patients into society and can affect their quality of life. Muscle strength has been shown to be a significant contributor to physical disability (more than the loss of dexterity) after stroke. It is therefore assumed that by increasing a patient’s muscle strength, functional everyday tasks will become easier.

PURPOSE: This study aimed to demonstrate that maximal strength training improves muscle strength, and to assess the effect on functional measures and walking economy amongst chronic stroke survivors.

METHODS: Baseline control trial. 10 patients acted as their own controls for 4 weeks, prior to an 8-week training intervention. Patients trained 3 days/week, 4 sets of 4 repetitions at 85-95% one repetition maximum in unilateral leg press and plantarflexion with an emphasis on maximal mobilization of force in the concentric phase.

RESULTS: Post training, leg press strength improved by 30.6kg (75%) and 17.8kg (86%), and plantarflexion strength by 35.5kg (89%) and 28.5kg (223%) for the unaffected and affected limbs respectively (all p=0.005). The 6-minute walk test improved by 13.9m (p=0.007) and the Timed Up and Go test time by 0.6 seconds (p=0.028). No changes were observed in the control period. There were no significant changes in walking economy, peak aerobic capacity, Four square step test or health related quality of life post training.

CONCLUSIONS: Chronic stroke patients could benefit from maximal strength training as it generates large increases in strength and some improvements in functional measures.

1311 Board #92 MAY 30 9:30 AM - 11:00 AM

Enhanced External Counter Pulsation Improves Endothelial Function in Patients With Left Ventricular Dysfunction

Randy W. Braith, FACSM, Darren P. Casey, C R. Conti, Wilmer W. Nichols, Calvin Y. Choi, Matheen A. Khuddus, Darren T. Beck. University of Florida, Gainesville, FL.

(No relationships reported)

PURPOSE: Enhanced external counterpulsation (EECP) improves arterial function, exercise tolerance, and angina symptoms in patients with coronary artery disease (CAD) and normal LV function. To date, however, studies have not elucidated the mechanism of action and the overall effects of EECP therapy in patients with LV dysfunction. The purpose of this study was to investigate the effects of EECP on endothelial function in peripheral muscular conduit arteries in patients with LV dysfunction.

METHODS: Patients with ischemic etiology of LV dysfunction (EF < 40%; n = 9), and age-matched patients with symptomatic CAD and normal LV function (n = 13), were studied after 35 1-hr sessions of EECP. Flow-mediated dilation of the brachial (bFMD) and femoral (fFMD) arteries was performed using high-resolution ultrasound imaging.

RESULTS: EECP treatment improved comparably (p > 0.05 between groups) the following FMD parameters in both the CAD and LV dysfunction groups, respectively: absolute bFMD (0.213 to 0.325 and 0.226 to 0.385 mm); relative bFMD (4.12 to 6.16 and 4.18 to 7.26 %); bFMD normalized for shear during the first 10 sec after cuff release (0.185 to 0.313 and 0.191 to 0.307 mm); absolute fFMD (0.198 to 0.264 and 0.194 to 0.226 mm); relative fFMD (2.77 to 3.66 and 2.73 to 3.19 %); fFMD normalized for shear (0.198 to 0.264 and 0.194 to 0.226 mm). EECP treatment also improved comparably (p > 0.05 between groups) plasma levels of the stable nitric oxide metabolites nitrate and nitrite (NOx) in both the CAD (19.4 to 30.1 μmol/L) and LV dysfunction (22.0 to 28.2 μmol/L) groups.

CONCLUSIONS: EECP treatment improves brachial and femoral artery endothelial function, and NOx levels similarly in patients with CAD possessing normal LV function and in patients with LV dysfunction. Our data suggest that peripheral vascular adaptations may be the specific mechanisms responsible for the beneficial clinical effects of EECP in patients with LV dysfunction.

1312 Board #93 MAY 30 9:30 AM - 11:00 AM

Evidence Of Reverse Left Ventricular Remodelling Following Exercise Training In Post-Myocardial Infarction Patients.

Gordon S. McGregor1, David C. Gaze2, Rob E. Shave3. 1UHCW NHS Trust, Coventry, United Kingdom. 2St George’s Healthcare NHS Trust, London, United Kingdom. 3Cardiff Metropolitan University, Cardiff, United Kingdom. (Sponsor: Keith George, FACSM)

(No relationships reported)

Raised serum NT-proBNP concentration is indicative of hemodynamic compromise following myocardial infarction (MI) and also in chronic heart failure (CHF). A reduction in NT-proBNP is evidence of successful treatment and may also indicate reverse left ventricular (LV) remodelling. Cardiac rehabilitation exercise training has been shown to reduce NT-proBNP in CHF, however, the effect of exercise training on resting and peak exercise NT-proBNP is less well characterised in post MI patients.

PURPOSE: To determine the effect of exercise training on resting and peak exercise NT-proBNP in post MI patients following successful percutaneous coronary intervention (PCI).

METHODS: Cardiopulmonary exercise testing (CPET) was undertaken in 31 male (56±10 yrs) clinically stable post MI (33±7 days) patients prior to (CPET1) and following (CPET 2) 10 weeks of exercise training. NT-proBNP measurements were determined from serum samples obtained at rest and at peak exercise via a peripheral venous cannula. Mixed modality (treadmill, cycle, rower, cross-trainer) supervised exercise training was conducted for 30-40 minutes twice weekly at 60-80% of V02 peak.

RESULTS: V02 peak significantly improved (3.1±2.6 ml.kg-1.min-1, P<0.0001) following 10 weeks of exercise training. Resting and peak NT-proBNP were significantly reduced by 178±419 ng/L (P<0.01) and 202±384 ng/L (P<0.001) respectively. NT-proBNP increased significantly (49±97 ng/L, P<0.001) from rest to peak exercise on CPET 1 but not on CPET 2 (25±77 ng/L, P>0.05) despite a greater exercise stimulus (147±26 vs 171±28 Watts, P<0.0001).

CONCLUSION: Consistent with findings in the HF population, exercise training improves V02 peak and lowers resting plasma NT-proBNP concentration in post MI patients. Furthermore, peak exercise NT-proBNP concentration is reduced. These reductions may potentially indicate reverse LV remodelling secondary to exercise training. However confirmation of this is required.

1313 Board #94 MAY 30 9:30 AM - 11:00 AM

Improvement in Exercise Capacity after Cardiac Rehabilitation is Independent of Ejection Fraction in Patients With Coronary Heart Disease

Aashish S. Contractor, Shraddha M. Khialani, Hetal D. Poptani, Anjali S. Zende, Priyanka M. Mehta. Asian Heart Institute, Mumbai, India.

(No relationships reported)

PURPOSE: A low ejection fraction (EF- <35%) risk stratifies an individual in the high risk category for cardiac events. It is a wide spread belief that these patients should not engage in intense physical activity. The objective of this study is to determine safety and efficacy of exercise training in coronary heart disease patients (CHD) with low EF, and to evaluate whether cardiac rehabilitation (CR) outcomes differ in patients with normal or reduced EF.

METHODS: 454 patients who were diagnosed to have CHD and enrolled for a 4 week CR program at the Asian Heart Institute; Mumbai formed a part of this study. Based on the EF at enrollement patients were divided into three groups: EF (>55%- 240 patients), EF (35%-54% -154 patients) and EF <35%-60 patients. Exercise capacity was determined using the 6 minute walk test at baseline. Patients exercised under telemetry supervision for 13 sessions over 4 weeks. Exercise was prescribed at 60%- 80% of predicted maximum heart rate and a rating of perceived exertion of 11-13 on Borg’s Scale. After 4 weeks the 6 minute walk test was repeated.

RESULTS: During the training period there were no adverse events reported. The results are tabulated as follows:

Table

Table

On comparing the change in the distance covered, no significant difference (p=0.694) was found between the three groups, indicating that improvement in the exercise capacity is independent of the EF.

CONCLUSIONS: Low EF patients with CHD can safely participate in a medically supervised exercise program and experience significant gains in exercise capacity equivalent to those with normal EF.

1314 Board #95 MAY 30 9:30 AM - 11:00 AM

Enhanced External Counter Pulsation Improves Exercise Capacity in Patients With Left Ventricular Dysfunction

Joseph C. Avery, Darren T. Beck, Darren P. Casey, C R. Conti, Wilmer W. Nichols, Calvin Y. Choi, Matheen A. Khuddus, Randy W. Braith, FACSM. University of Florida, Gainesville, FL.

(No relationships reported)

PURPOSE: Enhanced external counterpulsation (EECP) improves peak oxygen uptake (VO2peak) and exercise time to angina symptoms in patients with coronary artery disease (CAD) and normal LV function. However, the efficacy of EECP in patients with LV dysfunction remains controversial. The purpose of this study was to investigate the effects of EECP on VO2peak and exercise time to angina symptoms in patients with LV dysfunction.

METHODS: Patients with ischemic etiology of LV dysfunction (EF < 40%; n = 9), and age-matched patients with symptomatic CAD and normal LV function (n = 13), performed symptom-limited treadmill tests using a modified Naughton protocol and metabolic cart to determine VO2peak before and after 35 1-hr sessions of EECP.

RESULTS: EECP treatment improved comparably (p > 0.05 between groups) the following exercise parameters in both the CAD and LV dysfunction groups, respectively: VO2peak (17.0 to 19.8 and 13.9 to 16.3 ml/kg/min); total exercise time (615 to 813 and 493 to 659 sec): exercise time to angina (421 to 686 and 345 to 528 sec); peak heart rate (112 to 116 and 113 to 118 beats per min); peak subjective angina rating (2.8 to 1.7 and 1.8 to 1.5). EECP treatment also improved comparably (p > 0.05 between groups) the Canadian Cardiovascular Society (CCS) angina classifications in both the CAD (3.17 to 1.17) and LV dysfunction (3.14 to 1.29) groups.

CONCLUSIONS: EECP treatment improves VO2peak, total exercise time, exercise time to angina, and CCS angina classification to the same magnitude in patients with CAD possessing normal LV function and in patients with LV dysfunction. Our data indicate that EECP serves as ‘passive exercise’ and may be a useful intervention in individual patients with LV dysfunction who are poor candidates for aerobic exercise training.

1315 Board #96 MAY 30 9:30 AM - 11:00 AM

Field versus Laboratory Tests: Exercise Capacity Estimates in Elderly Patients with Coronary Artery Disease

Sandra Mandic1, Rob Walker1, Emily Stevens1, Bridget Mcilraith1, Sarah Featherston1, Edwin R. Nye1, Dianne Body2, Leanne Barclay2, Michael J A Williams1. 1University of Otago, Dunedin, New Zealand. 2Dunedin Public Hospital, Dunedin, New Zealand. (Sponsor: Jonathan Myers, FACSM)

(No relationships reported)

Compared to conventional laboratory exercise tests, timed walking tests are cheaper and a more simple alternative for an assessment of exercise capacity in cardiac rehabilitation participants. It remains unknown whether these tests provide an adequate estimate of peak oxygen consumption (VO2peak) in elderly individuals with coronary artery disease (CAD).

PURPOSE: To compare measured VO2peak during symptom-limited exercise testing versus estimated VO2peak derived from 6-minute walk test and incremental shuttle walk test in elderly CAD patients.

METHODS: Fifty-one elderly individuals with CAD (age: 73±6 years (range: 60 to 89), 16 (31%) women) completed the following assessments: 1) symptom-limited graded exercise test with expired gas analysis on a cycle ergometer using a ramp protocol; 2) incremental 10-meter shuttle walk test and 3) two 6-minute walk tests. VO2peak was estimated based on maximal walking speed (shuttle walk test) and distance, age, height, weight and rate pressure product (6-minute walk test) using published equations. Data were analysed using Pearson Product Moment correlation and paired t-test.

RESULTS: Measured VO2peak obtained during symptom-limited exercise test was significantly related to distance covered during 6-minute walk test (r=0.71, p<0.001) and duration of the incremental shuttle walk test (r=0.75, p<0.001). Estimated VO2peak based on 6-minute walk test data (16.0±4.4 ml/kg/min) and maximum walking speed achieved during shuttle walk test (14.7±1.9 ml/kg/min) were significantly lower compared to VO2peak measured during a symptom-limited exercise test (19.9±0.7 ml/kg/min; p<0.001 for both comparisons).

CONCLUSIONS: Distance covered during 6-minute walk test and duration of incremental shuttle walk test can be used for an assessment of functional capacity and monitoring of cardiac rehabilitation progress in elderly CAD patients. Estimation of VO2peak based on these tests underestimates exercise capacity in elderly CAD patients.

Supported by University of Otago Research Grant

1316 Board #97 MAY 30 9:30 AM - 11:00 AM

Coronary CT In Asymptomatic Subjects With Ventricular Repolarization Abnormalities During Maximal Exercise Test

Andrea Ermolao, Fausto Roman, Andrea Gasperetti, Maurizio Varnier, Eleonora Zanella, Marco Zaccaria. Università di Padova, Padova, Italy.

(No relationships reported)

Specific data are still lacking about the significance of repolarization abnormalities observed during exercise test in physically active asymptomatic subjects. Coronary CT is a highly sensitive diagnostic procedure able to confirm or exclude a significant coronary artery disease (CAD).

PURPOSE: To evaluate if the occurrence in asymptomatic subjects of ECG repolarization abnormalities potentially suggesting a CAD, is confirmed by coronary CT. Further, clarify if the likelihood of detecting CAD or coronary anomalies was related to the degree of the ECG abnormalities and to the prevalence of cardiovascular risk factors.

METHODS: Among 940 athletes performing a maximal exercise test for sport eligibility (age 30-60yrs), 44 asymptomatic subjects (4,7%, 39 men and 5 females, 45,2±7,1yrs) demonstrated repolarization abnormalities requiring the completion of a coronary CT. The exercise tests were classified into two categories: suspicious and positive for CAD.

RESULTS: The average maximal heart rate at exhaustion was 170,4±11,9. Risk factors evaluation demonstrated 10 subjects with hypertension, 23 subjects with hypercholesterolemia, 5 smokers. Among the 44 subjects 23 had a suspicious while 21 had a positive exercise test. The coronary CT demonstrated 6 subjects (13,6%) with a clinically significant CAD, 8 non-significant CAD, while 7 showed various coronary abnormalities. Considering the coronary CT as “gold standard”, the positive predictive value (PPV) of the exercise test for any coronary anomaly was 61,9% for positive exercise tests (47,7% including suspicious tests), while the PPV of positive tests for CAD was 47,6% (31,8% including suspicious tests). The association of late recovery abnormalities and cardiovascular risk factors improved the likelihood of CAD.

CONCLUSION: Our data suggest the clinical importance of slight repolarization abnormalities occurring at the peak of the exercise in asymptomatic middle-aged athletes, particularly when associated with the presence of cardiovascular risk factors. They also support the importance of a prolonged ECG monitoring during the late recovery phase. The PPV of a maximal exercise test in asymptomatic athletes appears lower than what observed in symptomatic subjects, however, about half of these subjects demonstrated some coronary anomalies.

1317 Board #98 MAY 30 9:30 AM - 11:00 AM

Effects of Exercise Training on Objectively Measured Physical Activity Levels in Heart Failure Patients

Daniel Clevenger, Peter H. Brubaker, FACSM, Joel Eggebeen, Dalane W. Kitzman. Wake Forest University, Winston-Salem, NC.

(No relationships reported)

Physical activity (PA) levels have not been quantified in the heart failure population, particularly heart failure patients with a preserved ejection fraction (HFpEF).

PURPOSE: To objectively measure PA in HFpEF patients and evaluate changes in levels after structured exercise training and weight loss interventions.

METHODS: Participants of this investigation were assessed at baseline and follow-up to an RCT of exercise and/or weight loss in HFpEF patients. Participants were randomized to one of four intervention groups for 20 weeks: Exercise Only (EO), Diet Only (DO), Exercise plus Diet (E+D), or Attention Control (AC).. Participants were asked to wear an accelerometer for 7 continuous days at baseline and then again during the final week of the intervention. The data was included if the participant wore the accelerometer for > 10 hours, of which included activity > 80% of the wear time, and had > 3 days of activity recorded. Each participant’s average steps/day, daily physical activity energy expenditure (PAEE) in kcal/min, minutes of light physical activity (LPA), and minutes of moderate/vigorous physical activity (MVPA) were analyzed.

RESULTS: Thirty-five subjects (77% female) with average age of 68.5±5.9 were included in the analysis. There were a significantly more steps per day in the E+D group compared to the AC group (6761 ± 2174 vs. 4052 ± 1083, respectively). There was a significant difference between the E+D, EO, DO, and AC, at follow-up for minutes of MVPA (31.0 ± 11.1, 11.6 ± 10.7, 14.2 ± 8.4, 9.9 ± 5.1, min/day respectively). There was also a significant difference for PAEE between the E+D and DO groups (280 ± 95 vs. 151 ± 52 kcal/day, respectively).

CONCLUSIONS: HFpEF patients randomized to the E+D group had significantly more minutes of MVPA compared to all of the other intervention groups and significantly greater number of steps/day than AC and more PAEE than DO. It appears that the weight loss, secondary to E+D, results in greater amounts of PA than EO and DO interventions for older HFpEF patients.

1318 Board #99 MAY 30 9:30 AM - 11:00 AM

Safety Of Cardiopulmonary Stress Testing In Patients With A Broad Range Of Cardiovascular Disorders

Erin West, Lora Raines, Daniel Forman, MD. Brigham & Women’s Hospital, Boston, MA.

(No relationships reported)

PURPOSE: While cardiopulmonary stress testing (CPX) has been well evaluated in heart failure patients, the utility and safety of these tests are not as well defined in other high-risk cardiovascular populations. The purpose of this study was to identify the rate of adverse outcomes during symptom limited CPX tests in a population of cardiovascular patients with a broad range of diagnoses.

METHODS: A retrospective data review was performed for CPX tests performed between July 2007 and July 2009. Patients with known cardiovascular disorders at the time of testing were included in the analysis. Peak functional capacity (VO2), peak respiratory exchange ratio (RER), and reason for test termination were collected. Adverse outcomes included significant hemodynamic abnormalities, arrhythmias, or ischemic changes.

RESULTS: The study population consisted of 815 patients (68% male) with the following cardiovascular diagnoses: dilated cardiomyopathy (41.8%), prior cardiac transplantation (25.2%), ischemic cardiomyopathy (12.3%), congenital/structural heart disease (3.9%), prior pulmonary embolism (3.4%), heart failure with preserved ejection fraction (2.9%), hypertrophic cardiomyopathy (1.8%), restrictive cardiomyopathy (1.7%), valvular disease (1.8%), and other cardiovascular disorders (5%). Mean peak VO2 was 15.9 ml/kg/min at a mean peak RER of 1.16. Eighty-three percent of patients achieved a peak RER >= 1.05. Thirty-seven tests were terminated prematurely by the supervising exercise physiologist for the following reasons: hypotensive response (1.7%), hypertensive response (1.2%), and significant arrhythmia (1.6%). No myocardial infarctions, cardiac arrests, or deaths occurred.

CONCLUSIONS: Even amongst a population of patients with significant cardiovascular disorders, symptom limited CPX tests can be performed in a safe and effective manner with proper screening and test supervision.

1319 Board #100 MAY 30 9:30 AM - 11:00 AM

Differences In Using The Same Supramaximal Verification Test Protocol For Treadmill And Cycle Ergometry

Christoph Otto1, Friederike Scharhag-Rosenberger2, Anja Carlsohn1, Jürgen Scharhag, FACSM3, Frank Mayer1. 1Potsdam University, Potsdam, Germany. 2University of Applied Science for Prevention and Health Management, Saarbrücken, Germany. 3University Hospital, Heidelberg, Germany.

(No relationships reported)

Verification testing to validate maximum oxygen uptake (VO2max) is used increasingly in performance testing. Different verification test protocols are applied at different points of time after incremental tests.

PURPOSE: The aim of this study was to examine whether there are substantial differences in using the same verification test protocol for treadmill and cycle ergometry. Furthermore, verification tests subsequent to incremental tests and on separate days should be compared.

METHODS: 10 recreational runners (f=4, m=6, 29 ± 6 y, 1.74 ± 0.06 m, 66 ± 7 kg, VO2max: 62 ± 6 ml/min/kg) and 10 male recreational cyclists (31 ± 5 y, 1.82 ± 0.05 m, 75 ± 9 kg, VO2max: 62 ± 5 ml/min/kg) underwent an incremental exercise test to exhaustion (E) either on the treadmill or the bicycle ergometer. After 10 min of rest a first verification test at 110% of vmax or Pmax (V1) was performed to exhaustion. 24h later a second verification test (V2) similar to V1 followed. Breath by breath gas exchange data were measured continuously. Capillary blood samples were collected at the end of each stage and at the point of exhaustion. Differences in E, V1 and V2 were tested by using ANOVA for repeated measures.

RESULTS: Runners’ VO2max (E: 62 ± 6 ml/min/kg, V1: 61 ± 7 ml/min/kg V2: 60 ± 6 ml/min/kg) was not significantly different between E and V1, while V2 was significantly lower than E (p<0.01). There was no statistically significant difference in the runners’ time to exhaustion (TTE; V1: 01:55 ± 00:15 min, V2: 02:05 ± 00:30 min). Cyclists’ VO2max (E: 62 ± 5 ml/min/kg, V1: 61 ± 6 ml/min/kg, V2: 63 ± 4 ml/min/kg) was significantly lower in V1 than in E (p<0.05) but there was no difference between E and V2. Cyclists’ TTE was significantly longer in V2 than in V1 (V1: 02:24 ± 00:20 min, V2: 03:23 ± 00:25 min, p<0.01). TTE was significantly longer in cyclists than in runners in V1 and V2 (p<0.01).

CONCLUSION: For recreational athletes, the time of verification testing seems less important since the observed differences in VO2max were irrelevantly small. Therefore, verification tests can be performed directly after incremental tests to ensure validity of maximum values. Due to the longer TTE in cyclists it seems needful to examine different test protocols for different exercise modes.

A-32 Free Communication/Poster - Diet and Exercise Interventions: Impact on Energy Balance

MAY 30, 2012 7:30 AM - 12:30 PM

ROOM: Exhibit Hall

1320 Board #101 MAY 30 11:00 AM - 12:30 PM

The Effect Of 2 Weeks Of Night Eating Restriction: A Randomized And Counter-balanced Study

Ed Christensen, Bruce W. Bailey, Larry A. Tucker, FACSM, James D. LeCheminant, FACSM. Brigham Young University, Provo, UT.

(No relationships reported)

PURPOSE: This study examined the effects of two weeks of night eating restriction on energy intake and body weight in young adult men.

METHODS: Eleven college-aged men were randomized to two weeks of normal eating (NE) and two weeks of night eating restriction (NER) in a counter-balanced fashion. There was a one week wash-out period between conditions. During NE, participants were asked to consume their normal diet, ad libitum. During NER, participants were asked to avoid all eating or kcal consumption from 7pm-6am but there were no restrictions on type or amount of food/beverage consumed. To assess diet, participants were asked to complete three randomly assigned dietary recalls during each week of the study. The National Cancer Institute’s Automated Self-administered 24-hour Dietary Recall (ASA24) was used to collect and analyze energy and macronutrient intake. Body weight was measured using a digital scale before and after each condition (NE and NER). All participants were screened and excluded for Night Eating Syndrome.

RESULTS: During NE, participants consumed 2,497 ± 514 kcal/d and during NER 2,115 ± 269 kcal/d (F=7.89; P=0.019). Dietary fat intake during NE was 105 ± 27 g/d and during NER was 86 ± 15 g/d (F=7.05; P=0.024). Carbohydrate intake during NE was 299± 67 g/d and during NER was 258 ± 64 g/d (F=7.05; P=0.024). There were no differences between the two conditions in protein, alcohol, or fiber intake (P>0.05). Body weight change during NE was 0.81 ± 0.87 and during NER was -0.53 ± 0.1.20 (F=13.09; P=0.005). Additional data collection is ongoing.

CONCLUSIONS: Restricting energy intake after 7pm may result in lower energy intake and potentially better short-term weight management than not limiting night eating. The difference in energy intake resulted from lower fat and carbohydrate intake during NER.

1321 Board #102 MAY 30 11:00 AM - 12:30 PM

Short Term Energy-Restricted Diets Improve Weight and Fat Loss Compared to Resistance Training

Elizabeth J. Sussman, Carol S. Johnston, Pamela D. Swan, FACSM. Arizona State University, Phoenix, AZ. (Sponsor: Pamela D. Swan, FACSM)

(No relationships reported)

Both energy expenditure and energy restriction are recommended for weight loss in obese populations.

PURPOSE This study examined the effects of energy restrictive diets (either high protein/low carbohydrate or high carbohydrate/low fat) or a resistance exercise training regimen on body weight, fat percentage and fasting resting metabolic rate (RMR) over a six week intervention.

METHODS Healthy adults (n = 25), stratified by age (mean = 37+11.5y), gender (4 M), and BMI (mean = 29.6±5.6 kg/m2), were randomly assigned to one of three groups: high protein (30% energy), low fat diet (PRO); high carbohydrate (60% energy), low fat diet (CHO); or resistance exercise training (EX). All foods consumed by the diet groups were prepared in a metabolic kitchen and designed to achieve a 1-2 pound weight loss per week. The EX group followed their usual diet but attended three supervised weight training sessions per week. EX was comprised of eight exercises over 45 minutes designed to achieve similar calorie deficit as that computed for the diet groups. Body composition was assessed by bioelectrical impedance and RMR was measured during 40 minutes of supine rest in a fasted state following 24 hours of no exercise. Changes in body weight, RMR and %fat between groups were analyzed using ANOVA.

RESULTS There were no differences between groups at baseline. Change in body weight and %fat was significantly improved (p < 0.001) for the PRO and CHO groups as compared to EX (Wt: -5.9%, -6.2%, -0.5%; Fat; -3.5%, -5.3%, + 1.2% respectively). There were no significant changes in RMR for any group.

CONCLUSION An energy-restricted caloric deficit produces greater weight loss and greater body fat loss in six weeks when compared to a resistance training exercise program inducing the same caloric deficit. It is possible that the EX group increased caloric intake during the intervention. Follow up is necessary to establish the long-term efficacy of these programs.

Supported by ASU Foundation.

1322 Board #103 MAY 30 11:00 AM - 12:30 PM

Optimizing Fat Oxidation in Untrained Women: The Competing Indices of Fitness versus Fat Loss

Harold C. Mayer, Deborah A. Beihl. Southern Adventist University, Collegedale, TN.

(No relationships reported)

Arresting the obesity epidemic is a high priority public health issue. This study explores factors affecting fat oxidation and resting metabolism in untrained overweight/obese women.

PURPOSE: The objective of this research was to explore the effects of a high fiber diet (The Full Plate Diet [FPD]) and exercise on resting metabolism, weight loss, and percent body fat loss.

METHODS: Overweight/obese (BMI between 25 -39), sedentary women (n=20) were recruited with a fiber intake <30g/day. Participants were randomized into three groups and followed for 8 weeks: FPD alone (FPD); FPD + continuous heart rate exercise (FPD+conEx); FPD + variable heart rate exercise (FPD+varEx). Pre- and post-study assessments included dietary analysis, resting metabolic rate, VO2 max treadmill test, and hydrostatic weight.

RESULTS: The high fiber FPD (consisting of 40 grams of whole food fiber daily) alone improved energy balance (from +339 to -226 kcals) without counting calories, but was inadequate to achieve fat (or weight) loss in the 8 weeks. The FPD+conEx group (training intensity 56.8% ± 14.1 verified via Polar heart rate monitors) saw a significant drop in (health measure) percent body fat (35.3% to 31.3%, p=.03), but no significant change in weight or (fitness measure) VO2max. The FPD+varEx group (training intensity = 67.7% ± 5.8 verified via Polar heart rate monitors) saw a significant drop in weight (3.7 lbs, p=.03) and significant improvement in fitness measure Relative VO2 max (20.8 to 24.1, p=0.016) and Absolute VO2 max (1.94 to 2.20, p=0.03), but no significant drop in percent body fat (39.9% to 38.2%, p=0.06). No group showed a statistically significant metabolic efficiency change in resting metabolic rate (RMR).

CONCLUSION: The Full Plate Diet helped participants to regulate energy balance without counting calories by reducing caloric intake, so they were more aligned with the resting energy expenditure (REE). Both increased fiber intake and low intensity exercise appear to be necessary to reduce percent body fat in untrained, overweight/obese participants. In fact, it appears that there is an inverse relationship between increased fitness and increased fat oxidation. Further research in a larger population over a longer period of time is needed to clarify this competing health - fitness relationship.

1323 Board #104 MAY 30 11:00 AM - 12:30 PM

Physiological and Psychosocial Benefits Associated with Weight Loss Programs: Changes in Body Weight, Social Physique Anxiety, and Exercise Enjoyment

Lynn A. Darby, FACSM1, Bonnie G. Berger1, Rob A. Carels1, Roberta L. Pohlman2. 1Bowling Green State University, Bowling Green, OH. 2Wright State University, Dayton, OH.

(No relationships reported)

Weight loss has been correlated with changes in social psychic anxiety (SPA) and exercise enjoyment (PACES) after a behavioral weight loss program (BWLP) (Darby, Berger & Carels, 2011).

PURPOSE: To further explore relationships among psychophysiological variables and to assess changes from before to after two different weight loss programs.

METHODS: Thirty-six participants who were overweight or obese completed anthropometric assessments [visceral adipose tissue (VAT) determined from sagittal abdominal diameter (SAD) (Parr & Haight, 2006)], physiological tests (submax walking GXTs), and dietary and activity (accelerometer) reports. They also completed the psychological inventories of the PACES (Physical Activity Enjoyment Scale; Kendzierski & DeCarlo, 1991) and SPA (Social Physique Anxiety; Hart, Leary, & Rejeski, 1989). All measures were completed before and after weight loss programs (BWLP LEARN, Brownell, 2004 or Transforming Your Life (TYL), Carels et al., 2011).

RESULTS: Completers included 10 men and 26 women (Age = 49 ±12 yr). Body weight (BW), VAT, BMI, GXT time were significantly different (p < 0.05) after 14-weeks. Mean scores pre- and post-program were as follows: BW (98.1 ±17.5, 93.3 ±18.2 kg), VAT (10.6 ±2.5, 8.6 ±2.5 lb), BMI (34.7 ±5.6, 32.3 ±5.6 kg/m2), and GXT time (563 ±216, 673 ±216 sec). Two-way ANOVAs (Time by Type of Program) indicated desirable changes in exercise enjoyment and social physique anxiety. Scores pre- and post-program were as follows: PACES (88.8 ±16.9, 97.7 ±14.1), and SPA (41.8 ±8.4, 37.6 ±8.7). There were no differences in PACES or SPA due to type of program, or sex. Not only did the PACES increase from beginning to end of the programs, pre- and post-values were significantly correlated with self-monitored average daily physical activity in minutes (r=.421, p=.02; r=.440, p=.01). Therefore, with participation in physical activity during the programs, there was a significant increase in exercise enjoyment and a decrease in anxiety. SPA scores at the beginning of the program were positively correlated with average energy intake during the program, however, lower SPA scores at the end of the program were not.

CONCLUSION: Weight loss programs, regardless of type, are associated with both desirable physiological and psychological changes.

1324 Board #105 MAY 30 11:00 AM - 12:30 PM

Weight Loss Induced by Exercise Preserves Resting Metabolic Rate: Midwest Exercise Trial II (MET II)

Jeffery J. Honas1, Richard A. Washburn, FACSM1, Stephen D. Herrmann1, Kate Lambourne1, Jaehoon Lee2, Joseph E. Donnelly, FACSM1. 1University of Kansas Medical Center, Kansas City, KS. 2University of Kansas, Lawrence, KS.

(No relationships reported)

Weight loss resulting from decreased energy intake has been associated with decreases in resting metabolic rate (RMR). The RMR response to weight loss by exercise without energy restriction is unclear. The inconsistent results on the effect of exercise induced weight loss on RMR may be due, in part, to variability in length, intensity, duration, and the level of supervision/verification of the exercise intervention.

PURPOSE: To evaluate the impact of weight loss induced by 2 levels of exercise energy expenditure (EEEx) on RMR.

METHODS: 91 sedentary, overweight/obese (BMI 25-39.9) young adults (18-30 yrs.; 55% female), were randomized to either 2,000 (N=36) or 3,000 (N=37) kcal/wk. of supervised treadmill exercise at 70-80% max heart rate (HR), or to a sedentary control group (N=18) for 10 mos. Participants were instructed not to alter energy intake. Exercise was progressed slowly in both exercise groups with participants reaching their target level at the end of mo. 4. The intensity and duration of all exercise sessions were verified by HR monitor. EEEx was assessed monthly by indirect calorimetry. RMR was assessed by indirect calorimetry at baseline and 10 mos. between the hrs. of 6-9 am, after a 12 hr. fast, and no exercise for 48 hrs.

RESULTS: Exercise at both 2000 or 3000 kcals/wk. resulted in clinically significant weight loss (2000 = - 4.2%, 3000 = - 5.3%) compared to the control group (+ 0.3%). Despite clinically significant weight loss, there were no significant decreases in RMR within or between exercise groups (2000 = -1.0%, 3000 = -1.6%; p =0.81) or between exercise and control (Exercise = - 1.3%, Control = +1.1%; p=0.48). The RMR response to exercise did not differ by gender (p=0.76).

CONCLUSIONS: Weight loss induced by exercise without energy restriction prevents the decreases in RMR typically associated with weight loss by energy restriction alone. These results suggest that exercise may be the preferable mode of weight loss for the prevention of weight regain.

Supported by NIH R01 DK49181

1325 Board #106 MAY 30 11:00 AM - 12:30 PM

Self-reported Diet And Exercise Are Similar in Clinic And Phone Based Interventions

Kate Lambourne, Stephen D. Herrmann, Jeffrey J. Honas, Richard A. Washburn, FACSM, Joseph E. Donnelly, FACSM. University of Kansas Medical Center, Kansas City, KS.

(No relationships reported)

Self-monitoring is a crucial component of diet and exercise interventions and has been associated with greater amounts of weight loss. It is not known whether self-monitoring data reported for diet and exercise are different when participants are not seen directly by a health educator, such as in a phone-based intervention.

PURPOSE: To compare self-reported use of pre-packaged meals (PMs-shakes/entrees), fruit and vegetable (F/V) consumption, and physical activity (minutes, pedometer steps/day) between individuals receiving a group-based intervention delivered face-to-face (FTF) or by conference call, and to determine if these measures are predictive of weight loss.

METHODS: 395 overweight and obese adults (BMI 25-39.9, age 18-65) were randomly assigned to a 6-month weight loss intervention delivered by conference call or FTF clinic. The intervention targeted 300 min/week of moderate-to-vigorous exercise and 10,000 steps/day. Energy intake was reduced to ∼1,200-1,500 kcal/day using PMs and F/V. Participants in both groups attended weekly clinics consisting of lessons on exercise, nutrition, and lifestyle modification. T-tests compared differences in the self-reported diet and exercise data between the phone and FTF groups. A regression analysis examined whether self-reported diet and physical activity data predicted weight loss after controlling for baseline weight and sex among the participants who completed the intervention.

RESULTS: No significant differences were observed in the self-reported diet and physical activity data between the phone and FTF group (p>0.05 for all comparisons). The number of pre-packaged shakes used (M=2.6, SD=0.34) and number of steps/day (M=8485, SD=2508) were significant predictors of weight loss (beta = 0.14, p = 0.02; beta = 0.17, p < 0.01, respectively; R2 = 0.28). The reported number of pre-packaged entrees, F/V, and minutes of physical activity were not significant predictors of weight loss.

CONCLUSION: Changes in self-reported diet and physical activity were similar between weight loss interventions delivered FTF or by conference call. The use of pre-packaged shakes and a pedometer to monitor physical activity resulted in greater weight loss, independent of the clinic delivery method.

Supported by NIH DK076063 and Health Management Resources, Boston, MA.

1326 Board #107 MAY 30 11:00 AM - 12:30 PM

Exercise Adherence By Energy Expenditure Level: Midwest Exercise Trial II (MET II)

Stephen D. Herrmann, Jeffery J. Honas, Richard A. Washburn, FACSM, Kate Lambourne, Joseph E. Donnelly, FACSM. University of Kansas Medical Center, Kansas City, KS.

(No relationships reported)

The current ACSM position stand on exercise and weight management recommends > 250 min/wk. of moderate intensity physical activity (∼1250-2450 kcal/wk. for a 100 kg individual at 3.0-5.9 METs) to achieve clinically significant weight loss. However, higher levels of exercise energy expenditure (EEEx) may have an adverse impact on adherence to an exercise protocol, and thus diminish the practicality of exercise recommendations for weight loss.

PURPOSE: To assess the effect of two levels of EEEx on adherence to 10 months of supervised exercise in sedentary, overweight/obese young adults.

METHODS: 115 sedentary, overweight/obese (BMI 25-39.9) young adults (18-30 yrs.; 55% female), were randomized to either 2,000 (N=53; 31 females) or 3,000 (N=62; 32 females) kcal/wk. of treadmill exercise at 70-80% max heart rate (HR), 5 days/wk. (supervised ≥4 days/wk.) over 10 months. Exercise was progressed slowly with participants reaching their target level at the end of month 4. EEEx was assessed monthly by indirect calorimetry to guide exercise prescription. The intensity and duration of all exercise sessions were verified by HR monitor. Compliance was assessed at 3 month intervals and defined as completing > 90% of scheduled exercise sessions at the target intensity and duration. Individuals falling below 90% compliance were dismissed from the study. Participants received $2,000 at study completion.

RESULTS: Overall, 64.3% of the sample completed the study per protocol. The majority of attrition (> 95%; n = 39) occurred during months 1-6. Those completing the study did not differ by EEEx (2,000 kcal/wk. = 69.8%, 3,000 kcal/wk. = 59.7%; χ2(1) = 1.28, p=0.26), sex (males = 71.2%, females = 58.7%; χ2(1) = 1.92, p=0.17); or baseline weight status (overweight = 64.9%, obese = 63.8%; χ2(1) = 0.02, p=0.90). Logistic regression indicated no significant impact of EEEx on adherence (p = 0.31) after controlling for sex, baseline weight and baseline fitness.

CONCLUSIONS: These results suggest that sedentary, overweight/obese young adults are capable of adhering to levels of EEEx associated with clinically significant weight loss. Additional studies to identify potential modifiable factors associated with improving adherence, particularly during the early months of an exercise intervention, are warranted.

Supported by NIH R01 DK49181

1327 Board #108 MAY 30 11:00 AM - 12:30 PM

Insulin Resistance and Body Fat Gains in Non-Diabetic Women: A Prospective Study

Jared M. Tucker1, Larry A. Tucker2. 1North Dakota State University, Fargo, ND. 2Brigham Young University, Provo, UT.

(No relationships reported)

PURPOSE: To determine the effect of insulin resistance (IR) on risk of gaining body fat (BF) over time in women, and to examine the potential confounding effects of age, education, initial BF, objectively measured physical activity, and energy intake.

METHODS: A prospective cohort study was conducted with 226 women completing the assessments at baseline and follow-up, 18 months later. IR was assessed using fasting blood insulin and glucose levels to calculate HOMA. Participants were divided into High IR (4th quartile) Moderate IR (2nd and 3rd quartiles) and Low IR (1st quartile) categories. BF was estimated using plethysmography (Bod Pod) at baseline and follow-up. Participants wore accelerometers and completed weighed food logs for 7 consecutive days to objectively index physical activity and energy intake, respectively.

RESULTS: On average, women in the High IR group decreased in BF (-0.48±3.60) from baseline to follow-up, whereas those in the Moderate (0.40±3.66) and Low IR (1.17±3.15) groups gained in BF in a dose-response manner over the 18 months (F=5.4, p=0.0211). Controlling for differences in age, education, physical activity, and energy intake had little impact on changes in BF across the insulin resistance groups, but adjusting for baseline BF nullified differences in body fat changes across the IR groups.

CONCLUSIONS: Women with High IR have less than half the risk of gaining substantial BF (>5%) over 18 months compared to women with low or moderate levels of IR. The decreased risk appears independent of potential confounders except for initial body fat levels, which seem to play a key role in the relationship.

1328 Board #109 MAY 30 11:00 AM - 12:30 PM

Changes In Physical Fitness And Body Composition During Inpatient Treatment Of Longstanding Eating Disorders

Eva M. Støa, Solfrid Bratland-Sanda. Telemark University College, Norway, Telemark, Norway.

(No relationships reported)

PURPOSE: The purpose of this study was to examine changes in aerobic fitness, muscular strength and body composition during inpatient treatment of underweight and normal weight patients with longstanding eating disorders (ED).

METHODS: Twenty-nine underweight (BMI<18.5, n=7) and normal weight (BMI ≥18.5, n=22) inpatients meeting the DSM-IV criteria for anorexia nervosa, bulimia nervosa or eating disorders not otherwise specified (mean (SD) age: 31.0 (9.0) yrs, ED duration: 14.9 (8.8) yrs, duration of treatment: 16.6 (5.5) weeks) completed this prospective naturalistic study. The treatment consisted of nutritional counseling, two weekly sessions of 60 min moderate intensive physical activity, and psychotherapy. Patients with BMI <18.5 aimed to increase body weight with 0.5 kg/week until BMI ≥18.5. Aerobic fitness, muscular strength (1RM) in lower and upper body and body composition were measured at admission and discharge.

RESULTS: There was a 28.5% increase in lower body muscular strength among the underweight patients, and 18.9% increase in upper body muscular strength among the normal weight patients from admission to discharge. No changes were detected in aerobic capacity. Total body mass increased with 7% among the underweight patients (from 46.2 to 49.5 kg, p<0.001), although three out of seven underweight patients were still underweight at discharge. No significant changes in total body mass were observed among the normal weight patients. Altering in body composition was observed, with an increase of 36% and 6% in fat mass among underweight and normal weight patients, respectively. Lean body mass increased among underweight, but not normal weight patients. Nine of the 29 patients had a body fat percentage exceeding healthy limits at admission; none of these showed decrease in body fat percentage at dicharge.

CONCLUSIONS: Several underweight patients were still underweight at discharge, whereas none of the patients with excess amounts of body fat managed to reduce body fat percentage. Increase in aerobic capacity would have been beneficial to especially patients with excess body fat. These results calls for a more individualized treatment approach to achieve a more optimal body composition among both underweight and normal weight patients with longstanding ED.

1329 Board #110 MAY 30 11:00 AM - 12:30 PM

Systemic Inflammation and Disease Risk Factors in Mexican-American Children after an Intensive Lifestyle Intervention

Whitney L. Breslin1, Craig A. Johnston2, Jennette P. Moreno2, Kelley Strohacker1, Katie C. Carpenter1, John P. Foreyt2, Brian K. McFarlin, FACSM1. 1University of Houston, Houston, TX. 2Baylor College of Medicine, Houston, TX. (Sponsor: Brian K McFarlin, FACSM)

(No relationships reported)

PURPOSE: Obesity is an independent risk factor for chronic disease. The prevalence of obesity is especially high among Mexican-American children. Peripheral blood monocytes are altered with obesity and may contribute to systemic inflammation that mediates the relationship between obesity and chronic disease. In addition, obesity alters the circulating levels of cytokines/chemokines that influence monocyte behavior. To evaluate the effects of a 6-month school-based healthy lifestyle program on systemic inflammation and disease risk markers in healthy weight (zBMI ≤ 85th percentile), overweight (zBMI=85th-95th percentile), and obese (zBMI ≥ 95th percentile) Mexican-American children.

METHODS: Participants were randomized to either a 6-month intensive intervention or self-help group. Pre- and post-intervention blood samples were analyzed for total monocyte concentration, pro-inflammatory monocyte concentration, and classic monocyte concentration via flow cytometry. Serum MCP-1, Fractalkine, IL-8, and TNF-α were measured using a Milliplex MagPix assay. Serum cholesterol, HDL, triglycerides, and glucose were measured using an enzymatic reagent kit.

RESULTS: At baseline, Total monocyte concentration (P=0.012), classic monocyte concentration (P=0.045), MCP-1 (P=0.015), and TNF- α (P=0.002) were significantly greater in obese children compared to healthy weight children. Also, overweight and obese children had elevated triglycerides (P=0.001) and reduced HDL (P=0.033) compared to healthy weight children. Post-intervention samples will be analyzed to determine the effect of the intervention on these markers of systemic inflammation and disease risk.

CONCLUSIONS: Elevations in circulating monocytes, MCP-1, and TNF-αhave been implicated in the development of obesity-related chronic disease in adults. School-based healthy lifestyle interventions that promote zBMI reduction may reduce systemic inflammation and prevent the future development of obesity-related chronic diseases.

1330 Board #111 MAY 30 11:00 AM - 12:30 PM

Daily Exercise and Caloric Restriction Impact Fibroblast Growth Factor-21 in Hyperphagic OLETF Rats

Justin A. Fletcher, Grace M. Meers, Jamal A. Ibdah, John P. Thyfault, R. Scott Rector. University of Missouri, Columbia, MO.

(No relationships reported)

PURPOSE: Chronic treatment with fibroblast growth factor-21 (FGF-21) favorably improves obesity and nonalcoholic fatty liver disease (NAFLD) outcomes; however, FGF-21 expression is paradoxically elevated in obese conditions. Here, we sought to determine the effects of obesity prevention by daily exercise (EX) vs. caloric restriction (CR) on hepatic FGF-21 in the hyperphagic, Otsuka Long-Evans Tokushima Fatty (OLETF) rat.

METHODS: At 4 wks of age, male OLETF rats (n=7-8/group) were randomized to groups of ad libitum fed, sedentary (OLETF-SED), voluntary wheel running exercise (OLETF-EX), or CR (OLETF-CR; 70% of SED) until 40 wks of age. Nonhyperphagic, Long-Evans Tokushima Otsuka (LETO-SED) rats served as controls.

RESULTS: Both daily EX and CR prevented obesity and NAFLD development observed in the OLETF-SED animals. This was associated with significantly (p<0.01) lower serum FGF-21 (∼80% lower) and hepatic FGF-21 mRNA expression (∼65% lower) in the OLETF-EX and OLETF-CR rats compared with OLETF-SED. However, hepatic FGF-21 protein content was reduced to the greatest extent in the OLETF-EX animals (50% of OLETF-SED) and did not differ between OLETF-SED and OLETF-CR. Further examination of hepatic FGF-21 signaling mediators revealed that the protein content of the hepatic co-factor of FGF-21 (β-Klotho), hepatic FGF-21 receptor 2 (FGFR2) mRNA expression, and hepatic FGF-21 receptor substrate 2 (FRS2) protein content were elevated (+30-50%, 60-100%, and ∼40%, respectively) in the OLETF-EX and OLETF-CR compared with OLETF-SED animals.

CONCLUSIONS: Daily exercise and caloric restriction modulate hepatic FGF-21 and its primary signaling mediators in the hyperphagic OLETF rat. Enhanced metabolic action of FGF-21 may partially explain the benefits of exercise and caloric restriction on NAFLD outcomes. Supported by NIH grant T32 AR 048523-07 and VHA-CDA2 IK2BX001299-01.

1331 Board #112 MAY 30 11:00 AM - 12:30 PM

Effects of Obesity and Exercise Training on Regional Adipose Tissue VEGF Production in Rats

Tongjian You, FACSM1, Beth Disanzo2. 1University of Massachusetts Boston, Boston, MA. 2State University of New York at Buffalo, Buffalo, NY.

(No relationships reported)

Vascular endothelial growth factor (VEGF) plays an important role in angiogenesis within adipose tissue. However, the effects of obesity and chronic exercise on regional adipose tissue VEGF production are still not well known.

PURPOSE: To compare the regional differences in VEGF production in subcutaneous and intra-abdominal adipose tissue, and investigate the effects of obesity and chronic exercise on adipose tissue VEGF production in rats.

METHODS: Lean (Fa/Fa) and obese (fa/fa) male Zucker rats at 2 months of age were randomly assigned to a sedentary and an exercise training group (lean sedentary: n=7, lean exercise: n=8, obese sedentary, n=7, obese exercise: n=8). The exercise group walked on a rat treadmill at 20 m/min for 60 min, 5 times per week for 8 weeks. Inguinal and epididymal adipose tissue samples were collected and in vitro VEGF secretion levels were determined.

RESULTS: In the whole cohort, VEGF secretion levels were higher in epididymal (13.20±3.93 ng/g protein) compared to inguinal (6.41±1.18 ng/g) adipose tissue (P<0.01). There were significant main effects of obesity on inguinal (lean: 2.66±0.23 ng/g, obese: 10.17±1.93 ng/g, P<0.01) and epididymal (lean: 6.92±1.85 ng/g, obese: 19.48±7.42 ng/g, P<0.05) adipose tissue VEGF secretion, and significant main effects of exercise training on epididymal adipose tissue VEGF secretion (sedentary: 5.80 ±0.77 ng/g, exercise: 19.68±7.05 ng/g, P<0.05). There were no obesity-exercise interactions on the variables.

CONCLUSIONS: Our results support that intra-abdominal adipose tissue releases more VEGF than subcutaneous adipose tissue, and both obesity and exercise training increase adipose tissue VEGF production in rats. Future studies are needed to identify the role of VEGF in angiogenesis in response to chronic exercise in obesity.

1332 Board #113 MAY 30 11:00 AM - 12:30 PM

The Supplement Of A-keto Acids Supports Diabetes Patients To Do Physical Exercise

Yuefei Liu, Tamara Spreng, Jürgen M. Steinacker, FACSM. Sports and Rehabilitation Medicine, Ulm, Germany.

(No relationships reported)

PURPOSE: Type 2 diabetes mellitus (DM) is one of the most important epidemic diseases that threaten the public health, and physical inactivity plays an important role in the development of DM. Therefore, to improve physical activity is of critical significance for dealing with DM. However, to do physical exercise brings about a variety of physiological changes including metabolic challenges that can reduce exercise tolerance. The aim of this study was to investigate supportive effects of nutritional supplement of a-keto acids (KAS) in patients with DM doing physical training.

METHODS: In the double-blinded, placebo-controlled study 30 patients with DM (non-insulin-dependent) were recruited (60 ± 10 yrs, 173 ± 8 cm, 91 ± 16 kg) and randomized into two groups (placebo and KAS, respectively). The subjects underwent 6 weeks of physical training on cycle-ergometers supervised by a professional assistant. The training program was composed of 3 obligatory sessions (3 x 15 min endurance training followed by 5 min high intensity strength training each) and voluntarily additional training. The agents with placebo or 0.2 g/kg/d a-keto acids (0.1g a-ketoglutarate and 0.1g branched-chain keto acids) were supplemented during the training phase and one following recovery week.

RESULTS: In the placebo group only 28 min/week voluntary training were performed while in the KAS group 78 min (P<0.05). With KAS the subjects attained higher maximum power output (224 vs 193 watts, P<0.05). A greater improvement in glucose control and Quantitativer Insulinsensitivität-Check-Index was achieved in the KAS group.

CONCLUSIONS: The supplement with a-keto acids in patients with DM during physical training could improve exercise tolerance and training effect along with beneficial effect on glucose control and insulin sensitivity. Thus, KAS supports patients with DM to do physical exercise.

1333 Board #114 MAY 30 11:00 AM - 12:30 PM

Similar Metabolite Shifts Following 75-Km Cycling Time Trials When Ingesting Bananas Or a Carbohydrate Beverage

David C. Nieman, FACSM1, Nicholas Gillitt2, Wei Sha3, Andrew Shanely1, Amy Knab1, Lynn Kam1. 1Appalachian State University, Kannapolis, NC. 2Dole Nutrition Research Institute, Kannapolis, NC. 3UNC-Charlotte, Bioinformatics Research Center, Kannapolis, NC.

(D.C. Nieman: Contracted Research - Including Principle Investigator; Dole Foods.)

PURPOSE: This study compared the acute effect of ingesting bananas (BAN) versus a 6% carbohydrate sports drink (CHO) on 75-km cycling performance and shifts in metabolites using metabolomics-based profiling.

DESIGN: Trained cyclists (N=14, age 37±1.9 y, Wattsmax 379±12.5) completed two 75-km cycling time trials (3 weeks apart, randomized, crossover) while ingesting BAN or CHO. Subjects cycled on CompuTrainers for 75-km at the fastest pace possible while ingesting 0.2 gm/kg carbohydrate from BAN or CHO every 15 min. Blood samples were taken pre-exercise, immediately following, and 1-h post-exercise, and analyzed for metabolic profiles using gas chromatography-mass spectrometry (GC-MS).

RESULTS: Performance time did not differ between BAN and CHO (2.41±0.06, 2.36±0.05 h, respectively, P=0.258). Of 103 metabolites detected, 56 had significant time effects following the 75-km cycling bouts, and only one (dopamine) had a pattern of change that differed between BAN and CHO. Score plots from the partial least squares discriminant analysis (PLS-DA) model visualized the global metabolic differences between pre-exercise, immediately post-exercise, and 1-h post-exercise, indicating a distinct separation between time points [R2Y(cum) = 0.869, Q2(cum) = 0.766]. Of the top 15 metabolites (ranked by both variable influence on the projection, VIP, and FDR adjusted p-values), five (2-hydroxybutyric acid, 2-aminobutyric acid, L-glutamic acid, L-methionine, and L-proline) were related to liver glutathione production, four (palmitoleic acid, palmitic acid, oleic acid, and heptadecanoic acid) to lipid metabolism, three (2,3,4-trihydroxybutanoic acid, D-fructose, and pyruvic acid) to carbohydrate metabolism, two (malic acid and succinic acid) were intermediates in the tricarboxylic acid cycle (TCA cycle), and one (L-isoleucine) was involved in branched chain amino acid metabolism.

CONCLUSIONS: With the exception of higher dopamine in BAN (a polyphenolic found in bananas), shifts in metabolites following BAN and CHO 75-km cycling time trials indicate a similar pattern of heightened production of glutathione and utilization of fuel substrates in several pathways including glycolysis, lipolysis, and amino acid catabolism.

Supported by a grant from Dole Foods

1334 Board #115 MAY 30 11:00 AM - 12:30 PM

High Physical Activity Counteracts the Altered Incretin Response to High Fructose Consumption

Jill A. Kanaley, FACSM1, Amy Bidwell2, Timothy Fairchild3. 1University of Missouri, Columbia, MO. 2Syracuse University, Syracuse, NY. 3Murdoch University, Perth, Australia.

(No relationships reported)

Fructose consumption has increased substantially in the past few decades and has been linked with metabolic maladaptations. Whether increased physical activity (PA) may confer protection against these maladaptations is yet to be determined. Although the glucose and insulin response to fructose ingestion has been well studied, the incretin response is not well understood.

PURPOSE: The purpose of this study was to determine the interaction between high fructose consumption and PA levels on the incretin response to a fructose rich meal in normal weight individuals.

METHODS: Twenty normal weight men and women (age: 21-30 yr) consumed an additional 64 g high fructose corn syrup for 14 days on 2 occasions. During these 14 days, subjects maintained either low PA (4,500 steps/day) or high PA (12,500 steps/day). Each condition was followed by a study day where subjects were given a fructose-rich meal (600 calorie mixed meal (45% carbohydrate [7.3% fructose], 40% fat, and 15% protein)) in the morning after a 12 h overnight fast, and blood was sampled at baseline and for 6 h after the meal. Samples were analyzed for glucagon-like peptide 1 (GLP-1), glucose-dependent insulinotropic peptide (GIP) and insulin concentrations. The incremental area under the curve (iAUC) was calculated to quantify the postprandial responses.

RESULTS: GIP concentrations showed a significant PA by fructose loading interaction (P=0.00) such that fructose increased the GIP iAUC levels from pre to post-loading more so in the physically inactive condition (pre 14,575±2339; post 30,431±3502 pg/ml) than in the high PA condition (pre 12,812±1303; post 14,938±1834 pg/ml). GLP-1 concentrations also demonstrated a significant interaction (P=0.05, n=13) such that high PA lowered the GLP-1 levels (pre 8203±1484; post 6248±1678 pg/ml) during fructose loading, while low PA resulted in an increase in GLP-1 levels (pre 6977±1508; post 8432±1539 pg/ml). The glucose and insulin response to the fructose challenge showed no changes with the loading or PA changes. There were no sex differences.

CONCLUSIONS: The combination of high fructose intake in conjunction with more sedentary behavior results a larger incretin response to a fructose-rich meal, while glucose levels are maintained. High PA appears to protect the incretin response to increased fructose loading.

A-33 Free Communication/Poster - Exercise Recovery Measures

MAY 30, 2012 7:30 AM - 12:30 PM

ROOM: Exhibit Hall

1335 Board #116 MAY 30 9:30 AM - 11:00 AM

The Influence of Aerobic Fitness on the Recovery of Peak Power Output

Mark Glaister, John R. Pattison, Bernadette Dancy, Gillian McInnes. St Mary’s University College, Twickenham, United Kingdom. (Sponsor: Gregory B Dwyer, FACSM)

(No relationships reported)

PURPOSE: The aim of this study was to evaluate the influence of aerobic fitness on the recovery of peak power output (PPO) following a maximal 30 s sprint.

METHODS: On separate occasions, 16 well-trained men (age: 21 ± 3 years; height: 1.84 ± 0.05 m; body mass: 78.8 ± 7.8 kg; and VO2max: 4.28 ± 0.54 L·min-1) performed a 30 s maximal sprint on a cycle ergometer, followed by a predetermined stationary rest period (5, 10, 20, 40, 80, or 160 s) and a subsequent 5 s sprint to determine the kinetics of PPO during recovery. On another occasion, oxygen uptake (VO2) was monitored during recovery from a 30 s sprint to enable the recovery kinetics of VO2 and PPO to be compared. In addition, subjects completed a VO2max test to evaluate the influence of this parameter on the recovery of PPO.

RESULTS: Comparison of the recovery kinetics of PPO and VO2 revealed no significant difference between the variables (F(1,15) = 3.16, p = 0.096); there was, however, a significant effect of time (F(5,75) = 374.44, p < 0.001), and a significant variable × time interaction (F(5,75) = 3.86, p = 0.004). Post hoc tests detected differences between the two variables at 20 s only (p < 0.05). Time constants for the kinetics of PPO and VO2 (54.0 ± 19.1 s and 51.8 ± 11.0 s, respectively) were poorly correlated (r = −0.17; p = 0.541), but were not significantly different (t(15) = 0.385, p = 0.705). There were no significant correlations between VO2max and the time constants of either PPO (r = 0.20; p = 0.455) or VO2 (r = −0.13; p = 0.641).

CONCLUSION: Despite many similarities, the results of this study suggest that the recovery of PPO may be faster than that of VO2 in the early phase of recovery following a maximal sprint; moreover, the speed of that recovery does not appear to be related to VO2max.

1336 Board #117 MAY 30 9:30 AM - 11:00 AM

The Effect of Short and Long Recovery Periods on the Contribution of Oxidative Processes to Energy Expenditure During Multiple Bouts of Supramaximal Exercise

Eric C. Olson1, Kenneth V. Christensen2, Adam Jajtner2, Jen Copeland2, Matt Unthank2, Joel B. Mitchell, FACSM2. 1Texas Christian University, Dallas, TX. 2Texas Christian University, Fort Worth, TX.

(No relationships reported)

The contribution of oxidative energy to multiple sprint exercise is of interest due to implications for the training needs of people engaging in anaerobic activities.

PURPOSE: The purpose of this study was to examine the effect of short and long active recovery durations on oxidative and anaerobic energy contributions during maximal instensity cycle ergometry.

METHODS: Six male subjects completed the study. After a VO2max test on the bicycle ergometer, each subject completed 2 conditions: a short recovery condition (SRC) and a long recovery condition (LRC). The SRC consisted of 10, 10-sec. supramaximal sprints with 30-sec. recovery periods. The LRC consisted of 10, 10-sec supramaximal sprints with 3-min. recovery periods. The load applied to the ergometer was 1.2 g/kg and the RPM during the sprints varied based on the maximal output. During recovery, no load was applied and subjects maintained a cadence of 80 RPM. VO2 was measured throughout both conditions and peak power and total work were calculated from two, 5-sec RPM averages generated during the sprints. Blood samples were taken pre-exercise, after sprints 4, 7, and 10, and 3 minutes post-exercise.

RESULTS: Peak power and total work were significantly greater (p < 0.05) in the LRC (1091.3 + 88.7 W and 1363.6 + 34.6 kg-m) compared to the SRC (915.3 + 109.2 W and 1161.6 + 33.9 kg-m). In addition, peak power decayed by 21.7% over the 10 sprints in the SRC compared to no decay in the LRC. Oxygen uptake averaged 28.3 + 0.9 ml/kg/min for the entirety of the LRC; whereas, in the SRC there was a large increase in oxygen uptake during the second sprint that remained elevated and averaged 47 + 1.5 ml/kg/min for the remaining sprints. There was no difference in blood lactate between conditions.

CONCLUSION: The heightened aerobic response and the lower work and power outputs seen in the SRC are suggestive of a decrement in both anaerobic glycolysis and phosphocreatine activity as successive sprints were completed. After repeated bouts of explosive exercise with short rest periods, oxidative processes play a more important role in energy production, most likely due to fatigue occurring in the anaerobic energy producing systems. These findings point to the need for enhancing the aerobic capacity of athletes engaging in consecutive high intensity bouts of exercise when rest intervals are short.

1337 Board #118 MAY 30 9:30 AM - 11:00 AM

Post-match Recovery In Elite Soccer Referees

Matthew Weston, Alan M. Batterham, FACSM. Teesside University, Middlesbrough, United Kingdom.

(No relationships reported)

The measurement of recovery following competition is required to facilitate the effective planning and timing of subsequent training sessions. Soccer refereeing at the elite-level represents a significant physical challenge. The effect of match intensity on post-match recovery, however, has yet to be investigated.

PURPOSE: To examine the effect of match intensity on recovery in elite soccer referees.

METHODS: Data were collected from 14 elite soccer referees for 194 English Premier and Football League matches (range: 5 to 21 matches). Internal match loads were RPE (CR-10 scale) and heart rate (HR) load, computed by multiplying the accumulated duration in each of five different HR zones by a multiplier for each zone and summating the results. Each match was analysed using a semi-automated match-analysis system. External match loads were the referees’ total distance covered (m) and the total high-speed running distance (m; running speed >19.8 km·-1). The referees’ recovery was recorded 30-min after waking on the day following their match, with a score of 0 representing poor recovery and 10 representing full recovery. A within-referee design was used to determine if high internal and external match loads were associated with low post-match recovery scores. Within-subject correlations between the referees’ measures of external and internal match load and post-match recovery (n=194) were examined, with 90% confidence intervals (CI) and effect sizes (Cohen’s d) also presented.

RESULTS: Match internal loads were 6.7 ± 1.5 and 304 ± 47 au for RPE and HR load, respectively. Match external loads were 11648 ± 609 and 1025 ± 338 m for total distance and high-speed running, respectively. Post-match recovery was 6.7 ± 1.2 au. There was a small correlation between post-match recovery and match RPE (r = -0.24: 90% CI -0.36 to -0.12, d = 0.49) and trivial correlations between recovery and total distance (r = 0.10: -0.02 to 0.22, d = 0.20), high-speed running (r = -0.03: -0.15 to 0.09, d = 0.06) and HR load (r = 0.06: -0.07 to 0.18, d = 0.11).

CONCLUSION: Elite soccer referees’ post-match psychophysiological recovery was influenced by the perceived exertion of their matches but not by their match running distances or match heart rates. Future research should investigate the effect of fitness levels and age on the post-match recovery process.

1338 Board #119 MAY 30 9:30 AM - 11:00 AM

Effects of Recovery Duration from Prior Heavy Exercise on VO2 Kinetics and Performance

Glen R. Belfry, Seung-Jun Park, William J. Karelsen, Juan M. Murias, Matthew D. Spencer, John M. Kowalchuk, Donald H. Paterson, FACSM. University of Western Ontario, London, ON, Canada.

(No relationships reported)

PURPOSE: To examine the effect of recovery duration (R) to heavy-intensity priming exercise (HVY1) on i) O2 uptake (VO2) kinetics during a subsequent HVY exercise bout (HVY2), and ii) VO2 kinetics and endurance time (TTF) during a subsequent maximal exercise bout (VHVY) performed to fatigue. It was hypothesized that VO2 kinetics would be faster in HVY2 than in HVY1, and that the TTF would be longer after priming exercise.

METHODS: Nine (22±2 yrs (mean±SD)) healthy and active males (n=4) and females (n=5) volunteered to participate. A ramp (25 W/min) test to fatigue was performed on a cycle ergometer on Day 1 to determine estimated lactate threshold (&thetas;L) and peak VO2 (VO2peak). Subjects returned on 8 separate occasions to perform the exercise protocols. These included transitions from 20 W to HVY1, HVY2 and VHVY. Each transition was separated by the same recovery period lasting 5 (R5), 12 (R12) or 25 min (R25) that were randomly assigned for each visit. The power outputs (PO) for HVY1 and HVY2 corresponded to 50% of the difference between the VO2 at &thetas;L and VO2peak (Δ50%); the PO for the VHVY endurance test was 92% of peak VO2 (271±77 W). Two repetitions of the protocol were performed for each of R5, R12 and R25. Two TTF tests with no priming exercise were also performed.

RESULTS: Peak VO2 and θL were 3.02±1.09 L·min-1 (at 293±97 W) and 2.08±1.02 L·min-1 (at 164±44 W), while Δ50% was 2.68±0.94 L·min-1 (at 228±69 W). With R5, VO2 kinetics (Mean Response Time) were faster (p<0.05) in HVY2 (49±10 s) and VHVY (47±13 s) compared to HVY1 (63±10 s); additionally, with R12, VO2 kinetics were faster (p<0.05) during VHVY (57±8 s) than in HVY1 (64±14 s). TTF was greater (P<0.05) after R25 (323±94 s) than no priming exercise (245±61 s), R5 (226±62 s), and 12 min R (255±65 s).

CONCLUSION: This study suggests after HVY priming exercise VO2 kinetics during a subsequent HVY exercise is faster when the R time is shorter (R5 vs R12 and R25). Improvements in TTF during very heavy-intensity exercise are observed with longer recovery (R25 vs R5 and R12), when enhanced VO2 kinetics are not seen. These data suggest that at exercise intensities above the lactate threshold, benefits of faster VO2 kinetics are attenuated by the production of fatigue-inducing metabolites that may require a longer recovery time to resolve.

1339 Board #120 MAY 30 9:30 AM - 11:00 AM

Recovery Of Heart Rate Variability Immediately After Endurance Exercises On Trained And Non-trained Individuals

Piia Kaikkonen1, Ari Nummela2, Heikki Rusko3. 1Tampere Research Center of Sports Medicine, Tampere, Finland. 2KIHU-Research Institute for Olympic Sports, Jyväskylä, Finland. 3University of Jyväskylä, Jyväskylä, Finland.

(No relationships reported)

PURPOSE: The goal of endurance training is to disturb homeostasis of the body to induce a training effect. Heart rate variability (HRV) has been used as a non-invasive technique to assess modulations in autonomic nervous system, regulating homeostasis. This study investigated the effects of different running exercises on immediate post-exercise HRV in individuals with different training backgrounds, to find out if immediate HRV recovery could offer additional information on disturbance of homeostasis, the training load and training effect of different exercises.

METHODS: Non-trained females (NT, n = 13, age 35 ± 3 years, VO2max 36 ± 3 ml/kg/min) and recreational trained males (T, n = 13, age 35 ± 5 years, VO2max 54 ± 4 ml/kg/min) performed three running exercises on a treadmill, separated at least by two days. In both group, the effects of increased intensity (NT from 50 to 74 %, T from 60 to 85 % of vVO2max) or the duration (NT from 3.5 to 7 km, T from 3 to 14 km) of the typical control exercise of the group was investigated. HRV data was collected during pre-exercise baseline, exercise and controlled recovery of 1 minute and analyzed with STFT method. The decrease in high frequency power, as an indicator of changes in vagal activation, from the baseline (HFPbl) to the first minute of the recovery (HFPrec1, % HFPbl) was investigated.

RESULTS: HFPbl in NT and T were 6.6 ± 1.1 and 7.8 ± 0.9 ln ms2, respectively. When compared to HFPbl, HFPrec1 varied from 41 ± 17 % (NT, 3.5 km at 74 % of vVO2max) to 83 ± 20 % (NT, 3.5 km at 50 % of vVO2max) after different exercises. Increased intensity of the typical exercise resulted in further decreased (P < 0.05) HFPrec1 in both NT (from 83 ± 20 to 41 ± 17 %) and T (from 60 ± 15 to 46 ± 18 %). Increased duration of typical exercise resulted in lower HFPrec1 in T (from 60 ± 15 to 47 ± 18 %, P < 0.05) while no change was seen in NT (83 ± 20 vs. 76 ± 26 %).

CONCLUSION: The effects of increased exercise intensity or duration, i.e. increased training load, of typical endurance exercises could be detected by HRV during the first recovery minute. However, small changes in duration or intensity may not necessarily induce changes on immediate HRV recovery. HRV during the first recovery minute seems to reflect training load of different exercises, and may be useful in evaluating the physiological training effects.

1340 Board #121 MAY 30 9:30 AM - 11:00 AM

Effects of Two-Minutes Active Recovery On A “Booster” VO2max Test Using Collegiate Female Soccer Players

Andy Bosak1, Matt Hawkey2, Thomas Andre1, Nathan Winn1. 1Georgia Southwestern State University, Americus, GA. 2Oklahoma State University, Stillwater, OK.

(No relationships reported)

Maximal oxygen consumption (VO2max) tests typically end at the point of volitional exhaustion. However, previous research with averagely fit individuals and highly fit NCAA Division II female athletes suggest that concluding a maximal treadmill test with 2min active recovery and allowing subjects to exercise a second time at the workload eliciting volitional exhaustion results in significantly greater VO2 max values (1.4% and 4.2% mean increase, respectively). The potential effects of this testing sequence (2min recovery) on VO2max treadmill tests has not been evaluated utilizing highly fit NCAA Division I elite female soccer players.

PURPOSE: To examine changes in VO2max values, in elite collegiate female soccer players, following 2min of active recovery at the conclusion of a treadmill GXT to volitional exhaustion.

METHODS: Twelve NCAA Division I female soccer players completed a max treadmill GXT until reaching volitional exhaustion (MAX1). Immediately following 2min active recovery (at 0% grade & 2.5 mph), each subject exercised to volitional exhaustion a second time (MAX2). MAX1 and MAX2 were compared using a paired T-test. Differences were considered significant at p ≤ 0.05.

RESULTS: No significant differences (p = 0.15) occurred between MAX1 (47.4 ± 4.7 ml/kg/min) and MAX2 (48.0 ± 5.3 ml/kg/min). Yet, the mean change from MAX1 to MAX2 following 2min active recovery was +1.3% with individual values ranging from -4.2% to +5.9%. Also, 66.7% of the subjects benefited (+3.3% mean increase) from the booster test with individual increases of +0.5% up to +5.9%.

CONCLUSION: Mean results suggest that 2min active recovery may not allow significantly greater VO2 max values to be achieved by highly fit NCAA Division I elite female soccer players during treadmill testing, yet 66.7% of the subjects increased their VO2max during the “booster max” treadmill protocol. Further research is needed to determine if fitness level, training experience, age, sport specificity, or other variables might affect this exercise testing protocol.

1341 Board #122 MAY 30 9:30 AM - 11:00 AM

In Vs. Out Of Water Recovery Methods, Performance And Inflammation Response: A Comparative Study

Mahdi Hossein Zadeh1, Valiollah Dabidi Roshan2, Hosein Babaei3, Vahid Shirinbayan2, Lars Arendt-Nielsen1. 1Aalborg University, Center for Sensory-Motor Interaction (SMI), Aalborg, Denmark. 2College of Physical Education and Sport Sciences, University of Mazandaran, Babolsar, Iran, Babolsar, Iran, Islamic Republic of. 3Islamic Azad University, Branch Sari, Sari, Iran, Sari, Iran, Islamic Republic of. (Sponsor: Jonathan Myers, FACSM)

(No relationships reported)

BACKGROUND: Repeated swimming sprints are commonly used to develop anaerobic performance in swimmers. The ability to recover from each high intensity sprint bout is an important factor for eliciting the best neuromuscular adaptations in order to attain the highest possible speed for each repetition, and therefore the best performance.

PURPOSE: To compare the effect of in (IN) vs. out of water (OUT) active recovery on cytokines (IL-6 and CRP), CK, lactate, and mean repeated sprint time (RSm).

METHODS: 16 volunteer male swimmers (age:19±4years; weight:75±12kg) were assigned to either IN vs. OUT recovery methods. Six 50-m sprint swimming bouts were performed with a 120-second interval of either IN or OUT recovery in between. Measurements were done at baseline and after third and sixth sprint swimming bout.

RESULTS: Serum IL-6, CRP and lactate levels (mg/dl) increased from baseline to the third and sixth bouts in both groups (IL-6: from 1.69±.78 to 2.40±1.047 and 3.86±1.81 vs. 1.84±.7 to 3.76±2.2 and 4.99±2.5, P<0.05, IN vs. OUT respectively); (CRP: from 0.39±0.21 to 1.26±0.69 and 1.95±1.14 vs. 0.37±0.25 to 1.29±0.42 and 1.75±0.56, P<0.05 IN vs. OUT respectively); and (Lactate: from 18.21±2 to 50.32±13 and 57.13±10 vs. 19.80±4 to 57.02±14 and 55.03±8, P<0.05 IN vs. OUT respectively). The CK level (U/l) and the RSm time (s) only increased in OUT (CK: from 245±109 to 294±117 and 302±121, P<0.05), (RSm: from 4.56±2.06 to 5.18±2.24 and 6.48±2.28, P<0.05).

CONCLUSION: Inflammatory and muscle damage markers are not affected by the IN vs. OUT recovery methods; however, out of water recovery might be associated with higher inflammatory responses. In-water recovery can improve performance compared with out-water recovery at the same intensity.

1342 Board #123 MAY 30 9:30 AM - 11:00 AM

Establishing An Active Recovery Protocol For Paralympic-level Swimming

Shane P. Esau, Jared R. Fletcher, Brian R. MacIntosh, FACSM. University of Calgary, Calgary, AB, Canada.

(No relationships reported)

Following a competitive event, subsequent performance is thought to be enhanced by an active recovery. In competitive Paralympic-level swimming, the athletes compete twice a day for 4-8 consecutive days. Blood lactate concentration ([BLa-]) has been used as a measure of the effectiveness of an active recovery. With this in mind, an active recovery protocol was investigated which could allow individualized prescribed active recovery.

PURPOSE: To determine [BLa-] clearance during warm-down of elite Paralympic swimmers and test this in competition circumstances.

Method: 29 elite Paralympic swimmers (age = 20±3 years) completed either a 150 or 300 meter time trial in the shortest time possible, five days before the IPC Swimming World Championships. The distance covered was based on the athlete’s main event at the World Championships. Post event [BLa-] was determined 5 minutes after the completion of the event. Subsequently, the athletes swam 1000 meters at a heart rate between 140-150 bpm as an active recovery. [BLa-] was measured every 200 meters of the active recovery. Individual [BLa-] clearance rates were determined from the half-life in exponential decay (t1/2). The following week, individual [BLa-] clearance rates were used to determine active recovery distance after each event during the IPC World Championships (WC).

RESULTS: Mean post time-trial [BLa-] was 9.3±3.4 mM. The mean [BLa-] t1/2 was 399.2±121 m. The distance required for the athletes [BLa-] to return to 4.0 mM and 2.0 mM based on the t1/2 was 470±190 m and 799±239 m, respectively. During the IPC WC, the distance required to return [BLa-] to at least 4.0 mM was 543±157 m. This distance was not significantly different than the active recovery distance calculated from the t1/2 and was significantly shorter than the athletes traditional active recovery distance (P<0.05).

CONCLUSION: The results suggest that determining [BLa-] t1/2 could be an effective method to determine an appropriate active recovery distance in elite Paralympic swimmers. Next it will be necessary to determine the appropriate target lactate for an active recovery.

1343 Board #124 MAY 30 9:30 AM - 11:00 AM

Weight Lifting Performance And Heart Rate Variability During A Training Recovery

Mu-Tsung Chen1, Jui-Lien Chen2, Chung-Yu Chen2. 1Shih-Chien University, Taipei, Taiwan. 2Taipei Physical Education College, Taipei, Taiwan. (Sponsor: Chia-Hua Kuo, FACSM)

(No relationships reported)

Weight training can cause muscle damage, which elicits a protracted repair process and resulted in greater muscle strength following approximately 48-72 h of recovery. It is currently unknown how the autonomic nervous system is modulated in relation to the strength training recovery.

PURPOSE: This study determined heart rate variability (HRV) during a 72-h recovery following a weight training bout for elite weight lifters, and its links to muscle strength, pain feeling and plasma creatine kinase level.

METHODS: After a 10-d detraining period, seven weight lifters performed a 2-h strength training program including back squat, seated shoulder press, dead lifts, and front squat. Weight lifting performance was evaluated at baseline (before training) and 3, 24, 48, 72 h following training. Electrocardiogram was continuously recorded for 5 min at rest in seated positions immediately before each strength performance measurement.

RESULTS: Weight lifting performance was recovered to baseline in approximately 24 h following training. Plasma creatine kinase level was peaked at 3 h following training and gradually decline for 72 h. Weight lifting performances were maximally increased above baseline at 72 h of recovery. Although the subjective pain feeling was not completely vanished, vagal activity (mirrored by natural log HF) was maximal at 72 h after training.

CONCLUSIONS: After an acute weight lifting training program, vagal activity dropped significantly and gradually elevated above baseline at 72 hour post training while the weight lifting performance was recovered to highest level.

1344 Board #125 MAY 30 9:30 AM - 11:00 AM

The Influence Of Recovery Time Following A Dynamic Warmup On Lower Body Balance And Flexibility

Lee Everett, Matt Beekley, FACSM. University of Indianapolis, Indianapolis, IN.

(No relationships reported)

Previous studies have reported improvements in flexibility following dynamic warm-ups. However, it is possible that the recovery period may influence subsequent performance changes.

PURPOSE: The purpose of this study was to examine the influence of recovery time following a dynamic warm up on lower body flexibility and balance.

METHODS: Twenty-eight healthy, recreationally active males [(mean ± SD) age, 21.3 ± 1.4 years; height, 178.0 ± 6.3 cm; weight, 80.9 ± 10.7 kg] volunteered for this study. The 28 participants reported engaging in a total of 6.9 ± 2.9 h·-1 of exercise. Each subject performed a dynamic warm-up which included an exercise routine that gradually progressed in intensity. Furthermore, each participant performed a pretest, and three post tests (0 min, 10 min, and 20 min). Flexibility was measured using both a straight leg raise test (SLR) for hamstring flexibility and Thomas test for hip flexor flexibility. Balance was measured in the number of ground contacts and balance time during a 30 second balance test using a balance wobble board. A one-way repeated measures ANOVA was used to analyze all dependent variables. Bonferonni adjusted pairwise comparisons were used as post hoc analysis. An alpha level of P ≤ 0.05 was set for statistical significance.

RESULTS: There was a significant decrease in hip flexor flexibility from 0 min post to 10 min (p = .015) and 20 min (p = .010) post warm up while also being significantly lower (p = 0.16) at 20 min post warm up compared to pre warm up values. Hamstring flexibility significantly increased from pre to 0 min post warm up (p < .001) while also significantly decreasing from 0 min post to both 10 min (p = .003) and 20 min (p < .001) post warm up. Hamstring flexibility was also significantly higher at 10 min post warm up (p = .006) when compared to pre warm up values. Balance contacts significantly decreased from pre to 10 min post warm up (p = .026), while balance time also significantly increased from pre to 10 min post warm up (p = .009).

CONCLUSION: A dynamic warm up may influence hamstring flexibility, however a longer recovery time following the warm up is less effective in maintaining hamstring and hip flexor flexibility improvements. Furthermore, the influence a dynamic warm up may have on balance may be minimal.

1345 Board #126 MAY 30 9:30 AM - 11:00 AM

Recovery And Soreness In Trained Females After An Exhaustive Resistance Training Protocol

Jason A. Campbell1, Phillip A. Bishop2. 1Murray State University, Murray, KY. 2The University of Alabama, Tuscaloosa, AL.

(No relationships reported)

PURPOSE: To examine the recovery capabilities and soreness profiles of 10 resistance-trained females (ages 19-35) following three sets to failure for eight resistance exercises.

METHODS: The intensity used to elicit responses was the participants’ 10-repetition maximum for each lift. Recovery was measured as the ability to replicate the total number of repetitions performed in the first set of the baseline workout compared to the first set total in subsequent workouts. Participants repeated the baseline session following 24, 48, 72, or 96 hours of passive recovery.

RESULTS: After 24 hours, the group mean for repetitions (10.0 ± 1.1 reps) was similar to baseline (10.7 ± 0.5 reps; p > 0.05). But at 48 hours and 72 hours, the group performed significantly better (11.5 ± 1.3 p = 0.01 and 11.4 ± 1.2, p = 0.004 reps for 48 and 72 hours, respectively) than at 24 hours. Soreness was also measured using a 100-mm visual analog scale (VAS). Soreness peaked at 24h and was significantly higher than baseline for all recovery periods (all p < 0.05). Additionally, at 48 hours, soreness was significantly correlated to the number of repetitions (r = -0.77, p = 0.01). Large inter-subject variability existed across all recovery periods for all variables.

CONCLUSIONS: These findings suggest that trained females can recover within 24 hours following an exhaustive resistance training protocol. Women were able to perform similarly to baseline at all time points despite experiencing a significant level of soreness.

1346 Board #127 MAY 30 9:30 AM - 11:00 AM

Examining Racial Differences in Sympathetic Overactivity Assessed During Recovery from Exercise in Obese Female Adolescents

Stacey L. Hall, R. Lee Franco, Mary K. Bowen, Ronald K. Evans, Edmond P. Wickham. Virginia Commonwealth University, Richmond, VA. (Sponsor: Edmund O Acevedo, FACSM)

(No relationships reported)

Sympathetic overactivity (SO) is associated with several disease states including type 2 diabetes, obesity, and hypertension. Black Americans have the highest risk for hypertension and black females have a greater prevalence in hypertension and obesity compared to white females.

PURPOSE: To evaluate differences in SO, as assessed by an exercise recovery index (ERI; heart rate/VO2 plateau), between black (BOA) and white obese female adolescents (WOA). An additional aim was to determine the association of ERI with insulin resistance (HOMA-IR), cardiovascular fitness (VO2peak) per fat free mass (FFM), systolic blood pressure (SBP), and percent body fat (%FAT) in both BOA and WOA.

METHODS: Fifty-six obese females volunteered to participate in this study. HOMA-IR, SBP and %FAT were assessed during resting conditions in BOA (n=45, 13.7±1.6 yrs, 38.1±6.1 kg/m2) and WOA (n=11, 13.3±2.2 yrs, 34.3±4.9 kg/m2). An ERI was calculated during a 5-minute passive recovery period immediately following a graded treadmill exercise test to exhaustion.

RESULTS: The ERI was significantly greater (29.7±6.1 vs. 23.9±3.1, P=0.004) in BOA compared to WOA females. Using multiple linear regression modeling, there was a significant independent association between ERI and VO2peak per FFM (r= -0.317, P=0.049) in BOA after controlling for HOMA-IR, SBP, and %FAT. HOMA-IR (r=0.232, P=0.155), SBP (r= -0.237, P=0.146), and %FAT (r=0.178, P=0.280) were not independently associated with ERI in BOA. Additionally, VO2peak per FFM (r= -0.075, P=0.860), HOMA-IR (r=0.112, P=0.792), SBP (r=0.060, P=0.887), and %FAT (r= -0.026, P=0.951) were not independently associated with ERI in WOA.

CONCLUSIONS: These results suggest that BOA females have greater SO, as assessed by an ERI, than WOA females. Understanding racial differences can contribute to both prevention and treatment of hypertension in obese female adolescents.

A-34 Free Communication/Poster - Military Physiology

MAY 30, 2012 7:30 AM - 12:30 PM

ROOM: Exhibit Hall

1347 Board #128 MAY 30 11:00 AM - 12:30 PM

Load Carriage Increases Exposure Time during Tactical Combat Movements

Andrew P. Hunt1, Aaron J. Silk2, Paul J. Tofari2. 1Defence Science and Technology Organisation, Melbourne, Australia. 2University of Wollongong, Wollongong, Australia. (Sponsor: Christopher Gore, FACSM)

(No relationships reported)

Dismounted combatants engaging an enemy force are required to move tactically between points of cover, involving repeated high-intensity short duration bounds.

PURPOSE: To quantify the effect of external load carriage on exposure time, the period of vulnerability to enemy fire as the combatant moves between points of cover, during a tactical combat movement simulation.

METHODS: Nineteen qualified Airfield Defence Guards (age 21.7±2.4 years, height 181.4±8.0 cm, body mass 81.0±9.0 kg) provided written informed consent to participate. Experimental procedures were approved by the Australian Defence Human Research Ethics Committee. The tactical movement simulation involved sixteen 6-m bounds commencing every 20 s, each starting from a prone position and ending in a kneeling position. The simulation was performed in five load conditions (A - E) ranging between 10-30 kg (5 kg increments) and comprised a replica weapon, chest webbing, protective vest, and helmet. During all trials participants were fitted with a global positioning device which contained a 10 Hz global positioning chip and nine inertial sensors. The data were analysed by custom software algorithms that were developed to objectively identify the start and end points of each bound. Repeated measures ANOVA assessed statistical differences between the load conditions.

RESULTS: Exposure time significantly increased as a function of external load (A 3.72±0.26 s, B 3.86 ±0.29 s, C 4.09 ±0.31 s, D 4.32 ±0.43 s, E 4.53 ±0.41 s, p<0.001). Peak velocity tended to decrease with increasing load, but this was not significant (A 2.65±0.26 m·s-1, B 2.67±0.26 m·s-1, C 2.58±0.30 m·s-1, D 2.51±0.30 m·s-1, E 2.42±0.25 m·s-1, p=0.095). However, a significantly greater time was required to reach peak velocity as external load increased (A 2.16±0.33, B 2.25±0.31, C 2.47±0.37, D 2.65±0.45, E 2.82±0.36, p<0.001). When normalised to exposure time, time to peak velocity did not differ, averaging 60% of the exposure duration.

CONCLUSION: Load carriage significantly impairs the ability to move between points of cover during tactical movement simulations. With technology advancing and dismounted combatants being required to carry increasing amounts of equipment, these findings highlight how additional load may impact survivability and ultimately mission outcomes.

1348 Board #129 MAY 30 11:00 AM - 12:30 PM

Foot Trajectory and Swing Time changes with Soldier Borne Loads in Walking and Running

Kari Loverro1, Leif Hasselquist2, Michael Brown2. 1Oakrigdge Institute for Science and Engineering, Belcamp, MD. 2Natick Soldier Research, Development, and Engineering Center, Natick, MA.

(No relationships reported)

Soldiers are often tasked with carry heavy loads. Little is known on how these loads affect trip avoidance. Previous researchers have used foot trajectory and swing time as predictors of tripping in the young and elderly adults.

PURPOSE: To compare foot trajectory and swing time of Soldiers during walking and running while carrying loads.

METHODS: Eight male soldiers walked (1.34m/s) or ran (2.24m/s) for 10 minutes on the treadmill with torso armor only (No RUCK: 17kg) and with a rucksack (RUCK: 40kg). Kinematic data was collected for 20s per condition; five strides from each trial were analyzed. Three landmarks on each foot (toe, 5th metatarsal head, and heel) were used to calculate foot trajectory and swing time. Foot trajectory variables were defined using minimum toe clearance (MTC), first and second peak in toe trajectory (TOE1 and TOE2), first peak in 5th metatarsal trajectory (MET5) and first peak in the heel trajectory (HEEL). Swing time was examined as percent stride time. Two-way repeated measures ANOVA were performed for each variable.

RESULTS: At both speeds, increased load significantly decreased swing time (walk: No RUCK: 37.7±0.3% RUCK: 35.9±0.5% p=0.005; run: No RUCK: 58.2±1.0% RUCK: 48.3±0.3% p<0.001). Increased speed for all load conditions significantly increased HEEL (No RUCK: walk: 31.7±0.4cm run: 36.3±0.7cm p<0.001, RUCK: walk: 31.8±0.5cm run: 34.8±0.5cm p<0.001) and MET5 (No RUCK: walk: 15.7±0.4cm run: 19.2±0.4cm p=0.001, RUCK: walk: 15.5±0.3cm run: 17.3±0.5cm p=0.011); but significantly decreased TOE2 (No RUCK: walk: 18.2±0.4cm run: 15.0±0.4cm p<0.001, RUCK: walk: 17.7±0.5cm run: 14.9±0.5cm p=0.006). During running, increased load significantly decreased HEEL (p=0.012) and MET5 (p=0.010). However, MTC (p=0.085) was not significantly affected by load or speed.

CONCLUSIONS: When carrying a load while ambulating, toe clearance appears to be preserved while swing time and other foot trajectory variables change. This implies that toe clearance is a crucial factor to keep consistent and Soldiers attempt to do this to prevent tripping while running with heavy loads. However, the decrease of the heel and 5th metatarsal head peaks; and reduction of swing time during running with heavy loads will decrease the ability of the Soldier to adjust their gait to prevent trips and falls on varied terrains.

1349 Board #130 MAY 30 11:00 AM - 12:30 PM

Improving Estimation of Energy Expenditure with Accelerometers during Military Training using a Field-Based Calibration Procedure

Sam D. Blacker, Fleur E. Horner, David M. Wilkinson, Denise M. Linnane, Peter I. Brown, Mark P. Rayson. Optimal Performance Ltd, Bristol, United Kingdom.

(No relationships reported)

Estimation of physical activity energy expenditure (PAEE) in military populations is an important measurement. The gold standard for estimating PAEE is doubly labelled water (DLW). However it is expensive and can only be used following observation periods greater than seven days. Accelerometers are low cost and their output (Physical Activity Counts; PAC) is often used in linear prediction models. However, the contribution of PAC in predicting PAEE can be low unless individual calibration is performed.

PURPOSE: To determine if simple field-based individual user calibrations could improve the relationship between accelerometer PAC and PAEE.

METHODS: Following ethical approval, 32 participants (16 female and 16 male, body mass 67.9 ± 12.4 kg, stature 1.71 ± 0.10 m, estimated VO2max 44.3 ± 8.5 mL·kg-1·min-1) volunteered for the study. During 10 days of military training, PAC were measured using a 3-dimensional accelerometer device worn around participants’ waist and PAEE was measured using DLW. Participants completed a multistage fitness test (MSFT) to estimate VO2max while wearing the accelerometers. Daily PAC were divided by PAC recorded during the first six levels of the MSFT (PACMSFT) to calibrate the outputs to individual participants’ movement patterns during the walking and running shuttles. Relationships between PAC and PACMSFT with PAEE were established using Pearson bivariate correlations. Linear regression was used to develop multivariable models to predict PAEE from body mass and PAC or PACMSFT. All data are expressed as mean ± one standard deviation and priori αwas set at p<0.05.

RESULTS: Mean 10 day PAC and PAEE were 536,341 ± 55,143 counts·day-1 and 1,600 ± 386 kcal·day-1, respectively. PAEE showed a moderate relationship with PAC (r=0.44, p=0.02) and a stronger relationship with PACMSFT (r=0.71, p<0.01). PAC did not contribute (p=0.10) to a multivariable model that included body mass to predict PAEE. However, PACMSFT contributed (p<0.01) to the multivariable model [PAEE= 505.48 - (body mass x 14.53) + (PACMSFT x 1.29), R2=0.68, SEE=227].

CONCLUSION: Calibrating accelerometer PAC to sub-maximal individual participant movements using a field-based fitness test provides a simple method for improving the prediction of PAEE during military training.

1350 Board #131 MAY 30 11:00 AM - 12:30 PM

Injuries among Army Recruits during Occupational Training for Military Police and Combat Engineers

Elizabeth Clearfield, Keith G. Hauret, Phillip Garrett, Ashly Westrick, Joseph J. Knapik, FACSM. Army Institute of Public Health, Edgewood, MD.

(No relationships reported)

New Army recruits who enlist as combat engineers (Engs) and military police (MPs) train 14 weeks and 19 weeks, respectively, at Fort Leonard Wood, MO. Training includes both physically demanding basic combat and occupational skill training. Injury incidence and rates among Eng and MP students have not been reported previously.

PURPOSE: To determine and compare demographics and injury rates for male students enrolled in 14-week Eng and 19-week MP occupational training courses that began between 1 October 2009 - 30 September 2010.

METHODS: Electronic rosters with demographics (age, height, weight) were obtained from the Army Training and Doctrine Command for all males who entered Eng and MP training during the survey period. Records for Eng and MP students were linked to injury data from the Defense Medical Surveillance System. Injuries were identified based on specific diagnostic (ICD-9) codes. Body mass index (BMI) was calculated as weight/height2. Demographics were compared by independent sample t-tests. Injury incidences (recruits with ≥1 injury/total recruits X 100%) and injury rates (injured/100 person-months [p-mos]) for Engs and MPs were compared using χ2 tests; rate ratios (RR) with 95% confidence intervals (CI) were calculated. All students were assumed to have completed the full training and to be at risk for injury for the full time.

RESULTS: There were 4,523 Engs and 4,090 MPs. Mean ± SD age, height, weight and BMI for Eng and MPs were, respectively, 21.2 ± 3.8 and 25.3 ± 3.4 years (p<0.01), 175.5 ± 7.0 and 175.8 ± 6.8 cm (p=0.02), 77.9 ± 14.2 and 78.7 ±14.2 kg (p=0.02), and 25.3 ± 4.1 and 25.4 ± 4.1 kg/m2 (p=0.13). Injury incidence for Engs was 41.7% and for MPs, 33.6% (p<0.01). The overall injury rate was 14.0/100 p-mos for Engs and 8.5/100 p-mos for MPs (RR [Eng/MPs] = 1.65, 95% CI: 1.54 to 1.77). Lower extremity overuse injury rate among Engs was 10.6/100 p-mos and among MPs was 5.7/100 p-mos (RR [Eng/MPs] =1.86, 95% CI: 1.71 to 2.02).

CONCLUSION: MP students were younger, taller and heavier than Eng students. Students in Eng training had higher overall injury and lower extremity overuse injury rates compared with MPs in training. Risk factors for injuries among Engs and MPs should be determined and injury prevention efforts focused on identified risk factors for students in these occupational training programs.

1351 Board #132 MAY 30 11:00 AM - 12:30 PM

Sleep and Activity Measurement in Search and Rescue Aircraft Crews Using Novel Sensing Technologies.

Gregory C. May, Giles Warrington, FACSM, John Burke, Sarah Colclough, Brendan Maloney, Ciaran O’ Cathain, Shauna O’ Connor O’ Connor, Stephen Ryan. Dublin City University, Glasnevin, Ireland.

(No relationships reported)

Helicopter search and rescue crews (SARC) remain on 24 hour alert. This requires the SARC to remain in a state of readiness and maximise sleep opportunities. When on duty, depending on their proximity to the SAR base, crew members may either sleep on-base or at home. These factors may lead to possible variations in the level of physical activity (PA), sleep duration (Sdur) and sleep efficiency (Sef).

PURPOSE: To investigate the levels of PA, Sdur, and Sef of members of the SARC during a 24 hour on-call shift using several novel sensing technologies. Method: Ten members of the Dublin SARC (mean ± SD: age 40 ± 5 years; height 1.76 ± 0.06m; mass 89.2 ± 14 kg; 5 on-base, 5 off-base) were instrumented with 2 tri-axial accelerometers (XL) and a Sensewear armband (SW) with an internal accelerometer (SWXL). The XL were placed on the right ankle and right hip with the SW placed on the left triceps. Data was recorded for a 26 hour period during which the subjects kept a written record of their activity. Total estimated energy expenditure (tEEE), Sef and Sdur were calculated for each sensor during the 24 hour period. Sleep periods were verified for each subject using a written activity log.

RESULTS: Group: Based on the placement location of the sensors (ankle; waist; triceps) significant differences were observed for tEEE (1093.9kcal ± 329.8kcal; 502kcal ± 211.5 kcal; 2371.1kcal ± 838.2kcal , p<0.01). Sleep indices calculated from the SW were seen to be significantly different to the XL data, but not between the XL units themselves (triceps vs. ankle; waist): Sef (72.8% ± 18.5% vs. 96.3% ± 2.6%; 97.3% ± 1.9%, p<0.01) and Sdur ( 257.9mins ± 80.1mins vs. 371.3mins ± 49.0mins; 379.6mins ± 53.9mins, p<0.01). Home vs Base: Significant differences were seen for tEEE for the SW (1907.0kcal ± 397.3kcal vs. 2835.2kcal ± 940.4kcal, p<0.01) and SWXL (193.8kcal ± 63.2kcal vs. 893.2kcal ± 564.2kcal, p<0.01). Similarly a significant difference was observed for Sdur (231.4mins ± 82.1mins; vs. 284.4mins ± 77mins, p<0.01) on the SW.

CONCLUSION: The location of the sensor utilised to measure PA and sleep indices in SARC members appears to play a vital role in determining the accuracy of measurement. The SW recorded significant differences in PA levels and Sdur between SARC on-base and off-base. Further research is required to determine if this holds true for a larger sample size.

1352 Board #133 MAY 30 11:00 AM - 12:30 PM

The Metabolic Cost of Walking and Running while Wearing Standard Military Gear or Running Shoes

Shannon R. Isom, Mariel Wenzel, Cory Whitmer, Michele Aquino, Nabil Boutagy, John W. Wygand, Robert M. Otto, FACSM. Adelphi University, Garden City, NY.

(No relationships reported)

Military personnel wear official military issue boots when deployed, however during non-combat base related activity, military personnel are permitted to wear ”go fasters” (running shoes) during their physical training. The military perception of using “go fasters” is based on empirical evidence from training exercises, but the energy cost of using either footwear has not been quantified.

PURPOSE: The purpose of this study is to measure the energy cost of walking and running in military boots and running shoes under conditions of no load and of simulated combat load (∼29.5kg).

METHODS: 6 current or veteran US Marines volunteered to participate in 8 randomly assigned steady state treadmill trials walking [W] (80.4 m/min 5% grade) and running [R] (160.8 m/min 0% grade), while oxygen consumption was obtained by open circuit spirometry. Data were collected at W and R in trials of unloaded with boots (UB), loaded state with boots (LB), unloaded with running shoes (US), & loaded with running shoes (LS). The loaded trial requires each subject to wear their own military issued boots (∼1.7 kg), a flak jacket without plates (∼4.0 kg), a pack (20.5 kg) and carry a rubber rifle (3.5 kg). During the last minute of each stage, rating of perceived exertion was obtained. A lactate (LA) sample was obtained at 3 minutes post exercise.

RESULTS: The mean energy cost for WUS, WUB, WLS & WLB trials: 17.4, 18.6, 22.8 & 23.9 mLO2/kg-min, HR: 101,114, 132 & 138 bpm, and LA: 2.9, 2.5, 2.4 & 2.3 mmol, respectively. The mean energy cost for RUS, RUB, RLS & RLB trials: 31.9, 32.2, 40.3 & 41.0 mLO2/kg/min, HR: 150, 164, 172 & 181 bpm, and LA: .4, 4.2, 5.7 & 7.5 mmol, respectively. Statistical analysis by ANOVA (p<.05) revealed no significant difference between footwear trials. However, the additional load of 29.7 kg required a significantly greater metabolic and cardiovascular response at both W and R trials.

CONCLUSIONS: The use of running shoes during non-combat physical training elicits a similar metabolic demand as the use of military boots. Therefore, the use of running shoes during physical training may be advantageous if injury potential is reduced.

1353 Board #134 MAY 30 11:00 AM - 12:30 PM

A Physiological Comparison of Three Backpack Frames During Treadmill Walking

Jana E. Hollins1, Kimberly A. Pribanic2, Brian Higginson3, Daniel P. Heil, FACSM1. 1Montana State University, Bozeman, MT. 2Mystery Ranch, LTD, Bozeman, MT. 3Gonzaga University, Spokane, WA.

(No relationships reported)

It is well known how heavy load positioning can influence cardiorespiratory (CR) responses when walking, but few studies have focused on how backpack frame design may impact these same parameters.

PURPOSE: This study compared CR variables for three backpack frames during treadmill walking, all of which are commonly used the U.S. Armed Forces.

METHODS: Backpack-experienced subjects (18 men, 4 women; Mean ± SD: 32±8 yrs, 79.7±7.7 kg) completed 4 successive 15-min walking trials at a fixed treadmill speed (80.5 m/min) and grade (2%). The Control trial (walking without a backpack) was always tested first, while the order of backpack frames tested were counterbalanced. Trials 2-4 corresponded to wearing one of 3 military backpack frames (MOLLE, FILBE, NICE), each of which was loaded with a 26.6 kg bag load. Subjects wore a portable metabolic measurement system for measuring oxygen uptake (VO2) and heart rate (HR), as well as carried a Rubber Ducky rifle in front, the total of which weighed another 4.1 kg (30.7 kg total load). CR variables were summarized at two time points (mins 6 and 13) for each trial, while blood lactate (BL) was measured during min 15. Data were evaluated using multivariate 2-factor RM ANOVA and Sheffe’s post-hoc (α=0.05).

RESULTS: Mean VO2 and HR values for the Control trial (Mean±SE: 13.7±0.4 ml/kg/min; 97±2 BPM) were significantly lower (P<0.05) than those for the MOLLE (16.5±0.3; 128±3), FILBE (16.6±0.4; 128±3), or NICE frames (15.9±0.4; 125±4), while there were no differences between frames. Mean BL for the FILBE trial (1.5±0.1 mmol/L) was significantly higher (P<0.05) than the Control (1.1±0.1) but not from either the MOLLE (1.3±0.1) or NICE (1.3±0.1) frames. The percent differences in VO2 of both FILBE (+4.4%) and MOLLE (+3.8%) frames from the NICE frame were practically significant.

CONCLUSION: The influence of backpack frame designs on CR and BL responses were generally small and non-significant. However, the FILBE frame trial elicited a slightly higher BL than the Control trial, and the difference in walking economy (i.e., VO2) between the NICE frame and the other frames (+3.8-4.4%) was greater than the 2.5% practical significance threshold. This suggests that both practical and statistical significance are most likely observed when these frames are worn for longer rather than shorter walking bouts.

1354 Board #135 MAY 30 11:00 AM - 12:30 PM

The Effects Of Wearing A Weighted Vest On Aerobic Efficiency, Muscular Strength and Body Composition: A Case Study

John W. Wygand, Raymond Peralta, Michele Aquino, Cory Whitmer, Mariel Wenzel, Shannon Isom, Anthony Lucic, Nabil Boutagy, Robert M. Otto, FACSM. Adelphi University, Garden City, NY.

(No relationships reported)

Operation Heavy Heart is a philanthropic effort by US Marine veterans in support of The Wounded Warrior Project. The objectives are to raise awareness and support for injured U.S. service members. Active duty military personnel are subject to unique and prolonged physiological stress. This includes chronic musculoskeletal loading with heavy combat gear for extended periods of time. Loading periods and subsequent unloading may be associated with specific physiological adaptations and may include alterations in body composition, energy expenditure, muscular strength and cardiovascular responses to exercise.

PURPOSE: To evaluate the effects of long term musculoskeletal loading on muscular strength, aerobic efficiency and body composition.

METHODS: Two US Marine veterans (Age 26.5 yr, Ht. 170 cm, Wt 76.5 kg) volunteered to participate in this nine month trial. Aerobic efficiency was measured by open circuit spirometry and heart rate by telemetry during steady state trials at 93.8 m/min and 10% grade. Body composition was measured by DEXA. Pull ups were performed to repetition max. with palms facing at shoulder width to a 3-1-3 cadence. Squats were performed to repetition max, on a Smith machine, with a load of 97.8 kg at a 3-1-3 cadence and a range of motion of 70-180 degrees of knee flexion. Each subject wore a 9.1 kg weighted vest during all daily activities except sleeping. All parameters were measured monthly during the nine months of weighted vest application and one month after they stopped wearing the vests.

RESULTS: .

Table

Table

CONCLUSION: Chronic, long term musculoskeletal loading is associated with improvements in body composition, upperbody strength and cardiovascular efficiency that persist for at least four weeks after the loading period.

1355 Board #136 MAY 30 11:00 AM - 12:30 PM

Effects of 4-Month Heat Exposure on Body Composition, Physical Performance and Serum Hormone Concentrations

Heikki Kyröläinen1, Matti Santtila2, Harri Lindholm3, Keijo Häkkinen1, Hanna Seikola1, Hannu Rintamäki3. 1University of Jyväskylä, Jyväskylä, Finland. 2Finnish Defence Forces, Helsinki, Finland. 3Finnish Institute of Occupational Health, Helsinki, Finland. (Sponsor: Paavo V. Komi, FACSM)

(No relationships reported)

Military operations in a hot environment together with body armor and helmet increase dramatically soldiers’ heat strain, which may decrease the operational readiness.

PURPOSE: To investigate changes in body composition, physical performance and serum hormone concentrations of soldiers during a 4-month deployment in physically demanding a high ambient air temperature environment (range from 18.1 to 37.7 °C, in vehicles even 57.0°C).

METHODS: 20 voluntary male soldiers (age 23 ± 1 yrs, height 1.80 ± 0.06 m, body mass 78.4 ± 11.5 kg) participated in the study. Whole body composition was measured by bioimpedance device and the cross-sectional area (CSA) of the thigh by magnetic resonance imaging. Physical performance was measured before and after the mission, which resulted in the energy deficit was 677 ± 1023 kcal/day. Basal serum hormone concentrations were measured before, middle and after of the deployment.

RESULTS: Body mass decreased by 3.5 % (p<0.05) but no changes were observed in fat % and CSA (subcutaneous fat and muscle) of the thigh. The mission did not cause any changes in the 12-min running distance (2785 ± 238 vs. 2749 ± 237 m, p=0.276) but increased MVC of the leg extensors (3042 ± 614 vs. 3277 ± 706 N, p<0.05). No changes were noticed in repeated squats and push-ups, while sit-ups and vertical jump improved (p<0.01-0.001). No significant alterations in basal cortisol (COR) or testosterone (TES) concentrations were observed but sex hormone-binding globulin (SHBG) concentration was higher (p < 0.05) at 2 (33 ± 10.2 nmol/L) and 4 months (31.2 ±10.3 nmol/L) compared with the initial values (25.5 ± 9.1 nmol/L). Insulin-like growth factor I (IGF-I) concentration was lower (p < 0.05) at 4 months (29.6 ± 6.5 nmol/L) compared with initial (33.1 ± 5.9 nmol/L) and middle (33.8 ± 5.1 nmol/L) concentrations.

CONCLUSIONS: Despite the heat stress and energy deficit, it seems that soldiers managed to retain their physical performance and basal COR and TES concentrations. Thus, it can be concluded that the overall stress during the mission was not very severe despite a decrease of the biomarker IGF-1. In addition, the type of physical activity, including strength training that soldiers often performed during the deployment might have played an important role in individual sustainability of the operational readiness.

A-35 Free Communication/Poster - Motor Control

MAY 30, 2012 7:30 AM - 12:30 PM

ROOM: Exhibit Hall

1356 Board #137 MAY 30 9:30 AM - 11:00 AM

An Investigation Of Age, Task Complexity, And Gender As Potential Moderators Of Attentional Focus Effects.

Peter J. Smith1, Kevin Becker2. 1Illinois State University, Normal, IL. 2University of Tennessee, Knoxville, TN. (Sponsor: Kristen Lagally, FACSM)

(No relationships reported)

A large body of research has suggested that focusing on the effects of a movement (external focus) is more effective than focusing on the movement itself (internal focus) for learning and performing motor skills (for reviews see Wulf, 2007; Wulf and Prinz, 2001). Recent research has suggested that age and task complexity may moderate this attentional focus effect.

PURPOSE: The present study examined the effectiveness of internal and external attentional foci for learning two novel locomotor skills varying in complexity.

METHODS: 48 children (ages 8-10) and 48 adults (ages 19-26) learned to ride a Double Pedalo either with or without stability handles while adopting either an internal or external focus of attention. Participants were instructed to either push their feet (internal focus) or the boards of the Pedalo (external focus) forward to make the Pedalo move. The dependent measure used was time to travel 7 meters.

RESULTS: For the simpler task, no attentional focus effects were elicited during either acquisition or retention. With the complex task, there were no significant attentional focus effects in acquisition, but in retention, an external focus of attention resulted in faster times than an internal focus, but only in males.

CONCLUSIONS: These findings further support the findings of Wulf, Toellner, and Shea (2007), suggesting that a certain degree of instability or error is necessary to elicit external focus benefits. In addition, they corroborate the findings of Wulf, Wächter, & Wortmann (2003) which suggested females and males may be differentially affected by attentional focus instructions.

1357 Board #138 MAY 30 9:30 AM - 11:00 AM

The Development Of General And Soccer-specific Perceptual Motor Skills During Adolescence

Tomi Vanttinen. Research Institute for Olympic Sports, Jyvaskyla, Finland.

(No relationships reported)

Expert-novice comparisons have indicated that perceptual motor skills are sport-specific. That is, the level of expertise is more important than the age itself in sports. However, it is not clear what is the dynamics of development of sport-specific perceptual skills compared to the development of general perceptual motor skills.

PURPOSE: To examine how general and soccer-specific perceptual motor skills develop during adolescence.

METHODS: The subjects of the present study were four age groups (10y, 12y, 14y and 16y) from a club team (n=74). Subject`s simple reaction time was measured with Wayne Membrane Saccadic Fixator and peripheral reaction time with Wayne Peripheral Awareness Trainer. A specific laboratory test was used to measure soccer-specific decision making time and choice reaction time during simultaneous ball handling. Age comparisons were made using one-way ANOVA with Tukey`s post hoc.

RESULTS: All perceptual motor skills improved with age (Table 1) from 10 to 16y but improvement was greater in soccer-specific perceptual skills (decision making time 79.0%, F73=49.315, p<0.001; choice reaction time 27.7%, F73=16.725, p<0.001) than in general perceptual motor skills (simple reaction time 17.6%, F73=6.371,p<0.01; peripheral reaction time 26.1%, F73=6.808, p<0.001).

CONCLUSIONS: These results suggest that soccer training provides training stimulus for soccer-specific perceptual skills that is beyond to be expected from the normal development of general perceptual skills. Decision making time was less than simple reaction time which means that the players ability to anticipate, not just react, is a key factor for success in soccer already at a young age.

Table 1

Table 1

1358 Board #139 MAY 30 9:30 AM - 11:00 AM

Analysis Of Inter-limb Force Coordination During Isometric Bilateral Grip Control Task

Chueh-Ho Lin, Wen-Hsu Sung, Li-Wei Chou, Shun-Hwa Wei. National Yang-Ming University, Taipei, Taiwan.

(No relationships reported)

Interhemisphere interaction plays an important role in motor performances, especially during inter-limb force control and coordination during exercise and activity of daily life. Simultaneous bilateral movement training can improve functional performance of the affected limb after CNS lesion. However, the effect of interhemisphere interactions on bilateral force control was still unclear.

PURPOSE: The aim of the preliminary study was to analyze interhemisphere interaction by investigating inter-limb force control and coordination during force-maintenance task.

METHODS: Seven female and seventeen male healthy adults participated in this study (mean age =23.3±3.4y/o). Subjects were asked to hold hand grip force of one hand at a given force level, then gradually decrease hand grip force of that hand while the other hand gradually increase hand grip force so that the sum of the total force from two hands maintained at the given force level. This task was performed at 10, 20 and 40% maximal force levels and was repeated in different order (right to left hand and left to right hand). The force outputs of the participant’s hands were recorded and analyzed. The timing of grip force cross point between two hands during force generating process was used to evaluate the force balance between both hands. Two-way analysis of variance was performed to determine the timing differences in handgrip force control under different conditions.

RESULTS: The results demonstrated that the force modulation timing in right to left hand was longer than in left to right hand condition at 10% (46.2±17.6% vs. 33.1±14.7%, p=.005), 20% (40.4±11.3% vs. 29.8±11.3%, p=.002) and 40% (40.7±13.7% vs. 27.9±14.6%, p=.002) isometric force-maintain tasks. The timing difference of target force-maintain task for both hands at 3 force levels was not significant different (18.9±14.6% vs. 13.1±11.6% vs. 17.6±9.5%, p=.229).

CONCLUSIONS: We concluded that left brain controlling right hand has greater control ability than right brain during bilateral force modulation. This finding could indicate interhemispheric interactions during bilateral limb control, which provide new information with implications for clinicians and therapists an evaluation parameter for inter-limbs coordination. Supported by the Taiwan NSC grant (NSC99-2221-E-010-002).

1359 Board #140 MAY 30 9:30 AM - 11:00 AM

Visuospatial Working Memory in Children with Poor Motor Coordination as Revealed by Event-Related Potential

Ming-Wei Chen1, Chia-Liang Tsai1, Chien-Yu Pan2, Tzu-Chi Chen1, Feng-Ying Chou3. 1National Cheng Kung University, Tainan, Taiwan. 2National Kaohsiung Normal University, Kaohsiung, Taiwan. 3Chi Mei Medical Center, Tainan, Taiwan.

(No relationships reported)

Children with poor motor coordination have been demonstrated to show an impairment in working memory in visuospatial domains. However, no studies have yet been conducted on the mechanisms of deficits of visuospatial working memory (VSWM) in children with poor motor coordination as revealed by event-related potential (ERP).

PURPOSE: The present study aimed to investigate the mechanisms of motor processing in brain activity underlying behavioral anomalies in children with poor motor coordination, and to compare them with those found in children with normal motor coordination when performing the VSWM task. METHOD: Twenty-five children with poor motor coordination and 25 age- and sex-matched children with normal motor coordination were identified with the Movement Assessment Battery for Children-Second Edition test. Each child simultaneously performed one spatial non-delay and two time-delayed spatial memory tasks (i.e., 3s-dealy and 6s-delay) with concomitant ERP recording. Behavioral and ERP data were statistically analyzed using repeated measures ANOVA.

RESULTS: Children with poor motor coordination had a significantly longer reaction time (879.93±67.13 vs. 829.07±52.43ms in 3s-delay task; 864.84±64.53 vs. 820.81±50.22ms in 6s-delay task; both p<.05) and lower accuracy rates (0.79±0.08 vs. 0.87±0.05 in 3s-delay task; 0.72±0.08 vs. 0.82±0.07 in 6s-delay task; both p<.05) than children with normal motor coordination in the two spatial memory tasks. With respect to the ERP components, children with poor motor coordination, as compared to children with normal motor coordination, showed smaller P3 (13.55±5.93 vs. 19.61±6.44μV in 3s-delay task; 16.54±7.83 vs. 21.05±7.59μV in 6s-delay task; both p<.05) and pSW (i.e., positive slow wave) (14.41±4.56 vs. 20.17±5.47μV in 3s-delay task; 15.40±5.75 vs. 19.38±7.93μV in 6s-delay task; both p<.05) amplitudes during the retrieval-process phase for later remembered items.

CONCLUSION: Children with poor motor coordination showed impairment when performing VSWM task and the mechanisms could allocate less resources and less effort for comparison of spatial locations and response selection.

Key words: motor coordination, visuospatial working memory, event-related potential, neuropsychology

1360 Board #141 MAY 30 9:30 AM - 11:00 AM

Effects of Exercise Intervention on Attention Networks in Children with Poor Motor Coordination: ERP Evidences

Tz-Chi Chen1, Chia-Liang Tsai1, Feng-Ying Chou1, Chien-Yu Pan2, Yu-Ting Tseng1. 1National Cheng Kung University, Tainan, Taiwan. 2National Kaohsiung Normal University, Tainan, Taiwan.

(No relationships reported)

Children with poor motor coordination (PMC) have been demonstrated to exhibit impaired inhibitory control capacity. However, no studies have been undertaken on the potential effects of exercise intervention on the attention networks of such individuals.

PURPOSE: The aim of the present study was to investigate the effects of soccer training on the inhibitory control of children with PMC, focusing on the event-related potential (ERP) components regarding early modality specific inhibition (N2) and late general inhibition (P3).

METHODS: Forty-two children were screened with the Movement Assessment Battery for Children test and categorized into those with normal motor coordination (n=14, control group) and those with PMC (n=28). Children with PMC were then quasi-randomly subdivided into either an exercise-intervention (EI) group (n=14) or a non-exercise-intervention (NEI) group (n=14). Before and after a ten-week training program (50-minute sessions, five times a week), all children performed the visuospatial attention task with centrally non-predictive gaze-directed cues with the lower extremities, while brain ERP were concurrently recorded. Repeated measure analysis of variance was used to analyze the training effect.

RESULTS: Before training, although the N2 component did not have any significant differences among the three groups, children with PMC, when compared to the control group, showed an impaired inhibitory control capacity (NEI: 49.15±37.99, EI: 43.04±23.80, Control: 10.00±17.99ms, p<.05), smaller P3 amplitude (NEI: 11.94±2.64, EI: 12.14±3.12, Control: 15.34±3.21μV, p<.05) and slower P3 latency (NEI: 335.73±30.34, EI: 332.37±36.28, Control: 300.12±26.30ms, p<.05) across conditions of the visuospatial attention orienting task. After training, beneficial effects emerged with regard to the strength of inhibitory control (NEI: 34.12±28.61 vs. EI: 11.62±16.83 and Control: 10.46±10.43ms, p<.05) and the P3 latency (NEI: 323.24±21.45 vs. EI: 292.89±24.43, Control: 299.96±24.43ms, p<.05) in the EI group.

CONCLUSION: The execution and acquisition of compound lower-limb motor skills during extensive soccer training for children with PMC seems to induce reinforced neuronal networks that enable faster cognitive processing.

1361 Board #142 MAY 30 9:30 AM - 11:00 AM

Changes In Mental Simulation Of Goal-directed Movements In Healthy Adults Aged 20- 89

Bouwien C. Smits-Engelsman. KULeuven, Leuven, Belgium.

(No relationships reported)

Mental simulation or imagery is a common mental training technique in sports and is gradually introduced for rehabilitation purposes in patient populations. Imagery research has demonstrated that motor imagery and motor execution share similar neural representations. However little is known about changes in the relation between the duration of actual and mental movements. We have recently shown that motor imagery training from a first person perspective in young healthy adults leads to improvements in performance of the executed movements (Herremans et al, 2011). However little is know about changes in the ability to represent actions during normal aging.

PURPOSE: To examine age-related similarities and differences in duration of mental and actual goal directed upper limb movements using a Fitts paradigm.

METHODS: 3 groups of adults performed the Radial Fitts Task with 5 Indexes of Difficulty (2.91-6.91) on a digitizer [young (20-29; n=39), senior (50-65; n=23) and elderly individuals (70-89 n=29); 97% right handed and 69% female].

RESULTS: Overall, the elderly group was significantly slower on the mental and executed tasks (F(1,2) 9,36, p<0.001, means 8.1, 7.5 and 12,7 seconds, respectively). However, no interaction with task condition (mental and executed) emerged, indicating that the mental and actual tasks slowed down comparably. In the second analysis the goodness of fit for the Index of Difficulty was determined. Here a highly significant group effect was found for the goodness of fit, testing for the adherence to Fitts’ Law, (F(1,2) 15,92, p<0.001; mean R2 was 0.70, 0.65 and 0.43, respectively). Elderly adapted their movement time less to the task difficulty. Again no interaction with task (mental and executed) was found.

CONCLUSIONS: The oldest group had a clear idea of their movement durations: they knew they were slow. However when they imagined to move they were also very slow, leading to good correlations between motor imagery and motor execution duration. Therefore we conclude that the ability to mentally represent goal directed actions is still intact.

References: Heremans E, Smits-Engelsman B, Caeyenberghs K, Vercruysse S, Nieuwboer A, Feys P, Helsen WF. Keeping an eye on imagery: the role of eye movements during motor imagery training. Neuroscience. 2011 Nov 10;195:37-44.

1362 Board #143 MAY 30 9:30 AM - 11:00 AM

Ankle Movement Discrimination Is Correlated with Sports Performance Levels

Jia Han1, Gordon Waddington2, Judith Anson2, Roger Adams3. 1University of Canberra; Shanghai University of Sport, ACT, Australia. 2University of Canberra, ACT, Australia. 3University of Sydney, Sydney, Australia.

(No relationships reported)

Numerous studies have tried to find evidence to suggest athletes specialized in particular sports have better ankle proprioceptive ability than athletes in other sports or non-athletes. However, the results in the literature are inconsistent. Although different testing methods and the diversity of testing range and planes of movement may account for this variability, other possible confounding variables such as years of training and sports performance levels have not been systematically investigated.

PURPOSE: To determine the relationship between ankle movement discrimination ability and years of training and sports performance levels.

METHODS: One hundred and twenty right handed, healthy young Chinese athletes (handedness was determined by the Edinburgh Handedness Test), without significant injuries during the past 6 months, mean 20.5 years, (range 18-25) and mean 8.2 years of current professional training, (range 2-15) across 6 sports (aerobic gymnastics (12F, 8 M), hand ball (9F, 11M), swimming (10F, 10M), soccer (7F, 13M), sports dancing (13F, 7M), badminton (8F, 12M)) were tested with a purpose-built ankle Active Movement Extent Discrimination Apparatus (AMEDA). Sports performance levels were determined by the athletes’ best record within the past year (level 3: national top 6 and above; level 2: national top 16; and level 1: national top 32 or regional top 3; mean level 1.7, range 1-3). Participants were tested in standing position with bare feet and undertook 50 trials (10 for each of 5 different inversion displacements) presented at random to the right ankle. Pearson correlations, with statistical significance at p<0.05, were calculated to estimate the relationship between movement discrimination scores and years of training and sports performance levels.

RESULTS: Enhanced ankle movement discrimination was positively correlated with higher levels of sports performance (p<0.001), but not with years of training (p>0.05).

CONCLUSION: These results are consistent with Ericsson’s (2006) hypothesis about deliberate practice underpinning high level sport performance. Given that higher level athletes have better ankle movement discrimination ability, ankle proprioception could possibly be considered as one of the measures used in sports talent identification testing.

1363 Board #144 MAY 30 9:30 AM - 11:00 AM

“Return To Driving”; Examining Driving Performance After Concussion

Maria T. Schultheis1, Preeti Sunderaraman1, Bradley J. Sandella2, Taylor Blake1, Jocelyn Ang1, Nazaneen Zahedi1, Danielle Martin1, Joy Ricasa2, Eugene S. Hong2. 1Drexel University, Philadelphia, PA. 2Drexel University College of Medicine, Philadelphia, PA.

(No relationships reported)

Management of concussions is commonly focused on “return to play” decision for athletes; however concussion may impact other activities, such as the ability to drive an automobile. Findings from other neurologically compromised populations have shown that deficits in information processing speed, working memory and executive functioning are related to changes in driving performance. Concussion research has implicated deficits in these same domains, yet little to no research has been done to examine the relationship between driving and concussion.

PURPOSE The current study compares driving performance (using a virtual reality driving simulator; VRDS) between individuals recently concussed (RC) and an age and gender-matched healthy control (HC) group.

METHODS Participants were recruited from consecutive referrals from 2 universities. Concussion was diagnosed by treating sports physician using the Zurich Concussion Consensus guidelines. Participants were seen for a 2.5 hr. testing session within the first 72 hours post-concussion. Testing included VRDS administration and a series of cognitive measures. The current findings are a part of a larger ongoing study. Sixteen individuals (11m, 5f; M=20 yrs old) were included. All participants were licensed, active drivers with greater than 2 years driving experience.

OUTCOME MEASURES The VRDS generates a variety of driving performance measures. For the current analysis, 2 driving variables; 1) center lane deviation and 2) mean speed were examined across 3 environments; 1) straight lane segment, 2) curve lane segments and 3) a complex driving task of following a truck. RESULTS Paired sample T-tests were used to compare group differences. In the straight driving task, the RC group spent more time outside their designated lane compared to HC (p = 0.005). In the curved driving task, the RC group drove significantly slower than the HC group (p = 0.02). During driving while following a truck, speed was significantly reduced for the RC group (p = 0.001). Exploratory correlational analysis between cognitive and VRDS measures revealed some significant relationships.

CONCLUSIONS These preliminary findings suggest that changes in driving behaviors may exist at 72 hours post-concussions and this may be related to cognitive performance. Supported by NIH Grant # 1R03HD064847

1364 Board #145 MAY 30 9:30 AM - 11:00 AM

Changes in Target Displacement and the Effects on Quiet Eye Duration

Michelle S. Okumura1, Robert R. Horn2, Michele M. Fisher2, Melissa G.F. Alexander2, Curtis T. Sylvester2. 1University of Georgia, Athens, GA. 2Montclair State University, Montclair, NJ.

(No relationships reported)

The role of gaze in aiming tasks (e.g., throwing a ball or shooting a gun) is an important characteristic of human performance, including certain aspects of sport and military performance. In aiming tasks, the final fixation of the eye before the initiation of a movement is called the quiet eye (QE) period. The location-suppression hypothesis proposes that the QE period is used to program the movement parameters (e.g., force, velocity, angle of release) during execution of an aiming task.

PURPOSE: To examine QE duration in response to directional and displacement-based changes in target location using a sky dart aiming task.

METHODS: 11 male participants (22.91±3.39 yrs) completed two sets of 46 throws to targets located on a grid on the floor. Each throw resulted in changes in target displacement in terms of direction (X-, Y-, or Z-axis) and distance (1-, 2-, or 3-increment changes on the grid). Changes in target displacement required the participant to scale the movement parameters on successive trials. The duration of the QE period was assessed on each trial using the vision-in-action approach.

RESULTS: A two-way ANOVA with repeated measures revealed a main effect for both direction, F(1.30, 13.05) = 204.85, p≤.001, and displacement, F(1.16,11.61) = 21.20, p≤.001. An interaction effect was also observed between target direction and target distance. For Y-axis changes, 3-increment changes resulted in longer QE duration (+0.039 s) than 1-increment changes. For Z-axis changes, 3-increment changes resulted in longer QE duration than both 1- and 2-increment changes (+0.144 s; +0.041 s), and 2-increment changes resulted in longer QE duration than 1-increment changes (+0.073 s).

CONCLUSION: Larger target displacements result in longer QE duration compared to smaller target displacements. Therefore, when throws require greater reprogramming of movement parameters, the QE period will be longer.

1365 Board #146 MAY 30 9:30 AM - 11:00 AM

Gross Motor Skills of Children and Adolescents with Mental Health Problems: a Pilot Study

Alain S. Comtois1, Claudia Verret1, Tommy Chevrette2, Jean P. Boucher, FACSM1, Jordan Lefebvre1, Émilia Kalinova1, Mario Leone2. 1University of Quebec in Montreal, Montreal, QC, Canada. 2University of Quebec in Chicoutimi, Chicoutimi, QC, Canada.

(No relationships reported)

PURPOSE: There is a growing body of literature providing evidence on the gross motor (GM) deficit of children having internalised or externalised mental health problems. Despite this, motor troubles are rarely reported in the primary diagnosis, and the impact of motor difficulties are not well documented. Thus, the goal was to evaluate the GM skills of children and adolescents having a mental health problem.

METHODS: Twenty-four boys and girls were divided into children and adolescents (n=11, n=5, n=6 and n=2, respectively). All participants had a primary diagnosis of mental health problem using DSM-IV and were referred into the study by a paediatrician. GM performance was evaluated with the UQAC-UQAM GM skills test battery comprising 12 items that assesses 5 GM qualities: Upper and Lower limb speed; Agility; Static and Dynamic Balance; Simple Reaction Time; and Coordination. Results were compared to percentile ratings gathered from 1494 boys and 1551 girls across 5 regions in Quebec all ranging in age 6.0 to 12.9 years.

RESULTS: The mean age of the children and adolescents, boys and girls, was 10.6±1.0 and 9.2±2.6, and 13.3±0.5 and 14.0±1.4 years, respectively. The children boys and girls were compared to their respective age groups (11.0 to 11.9 and 9.0 to 9.9 years, respectively), while both adolescent groups were compared to their gender respective 12.0 to 12.9 year age group. The children and adolescent boys and girls for weight, height and BMI scored in the 50th to 80th percentile. The upper and lower limb speed revealed that all boys and girls scored below the 20th percentile. The agility test percentile scores for children boys and adolescent girls was all within median range (40th to 70th percentile) while for the adolescent boys and children girls the range was within the 10 to 40th percentile. Static and dynamic balance indicated that all participants scored in the 10th to 30th percentile range. All participants scored in the 20th to 50th percentile range for the simple reaction time test, while for coordination the range was in the 10th to 50th percentile.

CONCLUSIONS: In this limited number of participants GM skills were all within the median range or lower (10th percentile) suggesting that mental health problems may lead to impaired GM skills development.

1366 Board #147 MAY 30 9:30 AM - 11:00 AM

Video Feedback on Advanced Wheelchair Skills Training for Individuals with Spinal Cord Injury

Xiang Ke1, Li-Shan Chang1, Michelle Nemeth2, Abhinanadan Batra1, Weerawat Limroongreungrat3, Yong Tai Wang, FACSM1. 1Georgia State University, Atlanta, GA. 2Shepherd Center, Atlanta, GA. 3Mahido University, Salaya, Thailand.

(No relationships reported)

PURPOSE: This study was to investigate the effectiveness of the implementation of immediate video feedback on advanced manual wheelchair skills training for individuals with spinal cord injury (SCI)

METHODS: Twenty-one manual wheelchair users with SCI level between T1 and L1 were recruited. Participants signed informed consent forms were matched (9 pairs) on gender and motor function level and randomized to the control group (using conventional training) or experimental group (using immediate video feedback training). Twelve participants were in the control group (33.22±11.33 yrs) and 9 in the experimental group (34.50±12.69 yrs). Each participant was expected to learn three advanced wheelchair skills (wheelie, ramping, and curbing) through four sessions: training session, competence test, retention test, and transfer test. The paired t-test was employed to determine the differences of learning and performing time (minutes), spotter intervention (times), occurrence of tip (times) and successful rate of performance between the two groups. A mixed-model ANOVA and Bonferroni pos hoc test were used to compare mean differences between the two groups on three wheelchair skills and four sessions

RESULTS: In the pair t-test, out of the 51 comparisons, only three comparisons were significantly different between the two groups. The experimental group had a significantly less performing time in wheelie competence test (1.33±.17 minutes vs. 2.05±.76 minutes, p<.05), a significantly more spotter intervention in curbing transfer test (6.15±1.3 times vs. 4.35±1.7 times, p<.05), and a significantly lower successful rate in curbing transfer test (33.5% vs. 81.5%, p<.05). In the mixed-model ANOVA analysis, no significant differences on ramping and wheelie skills across the four sessions were found between the two groups, except a significantly higher successful rate on the curbing in the control group than experimental group (81.4% vs. 56.7%, p<.05).

CONCLUSIONS: This study didn’t demonstrate the superiority of video feedback to conventional training for advanced manual wheelchair skills training for individuals with SCI. However, the immediate video feedback might be used to assist the conventional training for advanced wheelchair skills.

Supported by NIDRR Center Grant #: H133E080003

A-36 Free Communication/Poster - Nutritional Supplementation: Alternative Nutrients and Physiological Outcomes

MAY 30, 2012 7:30 AM - 12:30 PM

ROOM: Exhibit Hall

1367 Board #148 MAY 30 11:00 AM - 12:30 PM

Effects of Three Weeks of Carnitine Supplementation and High-intensity Interval Training on Endurance Performance

Kuei-Hui Chan, Shao-Zeng Hu, Kang-Hao Lu, Chun-Yi Shih. National Taiwan Sport University, Taoyuan, Taiwan.

(No relationships reported)

Carnitine has been presented to have the benefits on anti-oxidative and endurance capacity. High-intensity interval training (HIT) was demonstrated to increase the endurance capacity and oxidative stress.

PURPOSE: To investigate the effects of three weeks of carnitine supplementation and HIT combination on aerobic capacity and oxidative stress.

METHODS: Ten untrained healthy males were recruited and randomly assigned into carnitine group (25.0+4.4 yr, 80.3+8.5 kg, 170.2+4.3 kg) or placebo group (22.4+3.3 yr, 69.1+11.2 kg, 172.2+6.3 kg). Subjects received carnitine or placebo supplementation 2 g per day for 3 weeks. All subjects also performed the HIT three per week. The HIT protocol contains 10 bouts of cycling trail at 90%VO2max, with each bout lasting 2 min and separated by 1 min of rest. The increment running tests and high-intensity interval exercise tests were conducted before and after supplementation. The VO2max and time to fatigue were measured by the increment running test. Blood samples were drawn before test, immediately after test, 1 h and 3 h after test of high-intensity interval exercise test to determine the activities or concentrations of creatine kinase (CK) and uric acid.

RESULTS: After 3 week of supplementation, VO2max (43.0+2.4 vs. 37.6+5.0 mL/min/kg) and time to fatigue (1563.0+221.0 vs. 1496.0+207.6 sec) significantly increased (p<0.05) in carnitine group. However, there were no differences in placebo group. In carnitine group, the values of blood CK and uric acid in high-intensity interval exercise test before supplementation still elevated at 3 h after test (170.0+44.6 vs. 160.8+32.8 U/L for CK and 8.88+2.16 vs. 7.88+2.0 mg/dL for uric acid, p<0.05). But the values returned to baseline after 3 weeks of supplementation (184.8+48.7 vs. 174.6+43.5 U/L for CK and 7.82+1.98 vs. 7.74+1.95 mg/dL for uric acid).

CONCLUSION: Carnitine supplementation during HIT is benefit to aerobic capacity and recovery of high-intensity interval exercise.

Supported by NTSU Grant 99D014.

1368 Board #149 MAY 30 11:00 AM - 12:30 PM

Effects Of Chronic Green Tea Extract Supplementation On Substrate Utilization And Time-trial Performance

Brian J. Martin, J. Albert Bartolini, Taylor S. Thurston, Nicholas W. Aguirre, Benjamin M. Kamel, Jared W. Coburn, FACSM, Lee E. Brown, FACSM, Daniela A. Rubin, Daniel A. Judelson, FACSM. California State University, Fullerton, Fullerton, CA.

(No relationships reported)

Supplementation with green tea extract (GTE) has been shown to increase fat oxidation at rest and during moderate intensity exercise. Despite its potential ergogenic utility, little work examines if or how GTE supplementation influences metabolic function and performance during time-trial exercise in humans.

PURPOSE: To examine the effects of chronic GTE supplementation on markers of substrate oxidation and physiological stress during 1 h constant load submaximal exercise and performance during a subsequent 10 km time-trial.

METHODS: Nine male participants with previous 10 km or half-marathon race experience volunteered (age = 27 ± 5 y, height = 174.8 ± 7.2 cm, mass = 71.6 ± 6.1 kg, body fat = 10.3 ± 2.7%, and VO2max = 64.2 ± 6.0 ml·kg-1·min-1). In a double-blind crossover design, subjects supplemented with GTE (725 mg) or placebo for 14 days then performed 1 h of treadmill running at 50% VO2max immediately followed by a 10 km time-trial. Investigators collected 1) expired gasses during the constant load exercise only, and 2) heart rate (HR) and ratings of perceived exertion (RPE) throughout both bouts. A 14 day washout separated supplementation periods.

RESULTS: No significant differences existed between trials in VO2 or respiratory exchange ratio during constant load exercise. GTE supplementation also failed to affect HR and RPE during either constant load exercise or time-trial. Time-trial performance was similar between trials (placebo = 47.9 ± 6.7 min, GTE = 46.6 ± 5.8 min).

CONCLUSIONS: Supplementation with GTE did not affect metabolic, physiological or perceptual variables during moderate intensity exercise. Additionally, no differences were observed in performance during a 10 km time-trial. These data suggest chronic GTE supplementation does not alter substrate utilization or increase endurance exercise performance in trained individuals.

1369 Board #150 MAY 30 11:00 AM - 12:30 PM

Green Tea Consumption And Risk Of Prostate Cancer: Meta-analysis Of Epidemiologic Studies

Byungsung Kim, Seunghyun Lee. Kyunghee University, Seoul, Korea, Republic of.

(No relationships reported)

Purpose Worldwide, prostate cancer has the second highest incidence of all male cancers. Prostate cancer incidence and mortality varies widely between geographic regions, with overall rates in USA being nearly six-times higher than that of Asian countries. This variation suggests that prostate cancer may be linked to lifestyle-related factors, particularly dietary factors. Several epidemiologic studies have focused on the lower incidence of prostate cancer in Asian countries where green tea consumption is high. We undertook a meta-analysis to verify the relation between green tea consumption and prostate cancer.

Methods We performed meta-analysis with the search of MEDLINE (PubMed), EMBASE and Cochrane Library, using the keywords “green tea” or “polyphenols” or “catechins” for the exposure factors, and “prostate cancer” for the outcome factors. Studies included in this review are prospective cohort and case-control studies published between 1966 and July 2010. The search was limited to English language.

Results A total of 20 articles were identified for this review. Only 6 studies met the inclusion criteria. When using all the case-control and cohort studies, the pooled RR of prostate cancer for the highest vs lowest category of green tea consumption was 0.82 (95% CI, 0.75-1.07). In case-control studies, green tea drinking habit showed preventive effect on prostate cancer with the odds ratio of 0.40 (95% CI, 0.17-0.92). When cohort studies were pooled, no significant association was seen between green tea consumption and prostate cancer. (RR, 1.08 CI, 0.89-1.32)

Conclusion Green tea consumption was not associated with the risk of prostate cancer in this meta-analysis. Further cohort studies are needed.

1370 Board #151 Abstract Withdrawn

1371 Board #152 MAY 30 11:00 AM - 12:30 PM

American Ginseng And Swimming Combined Effect On Glucose Tolerance In Fructose-Fed Rats

HUNG-MIN TSENG1, Chung-hsiang Yang1, Kuei-yu Chien2, Mei-chich Hsu2. 1National Taipei University of Education, Taipei, Taiwan. 2National Taiwan Sport University, Taoyuan, Taiwan. (Sponsor: Chia-Hua Kuo, FACSM)

(No relationships reported)

PURPOSE: The purpose of the present study was to investigate the combined effect of American ginseng and swimming on regulation of blood glucose homeostasis in fructose-fed insulin resistance rats.

METHODS: Seventy Wistar male rats were divided into ten groups. First group served as control, and remaining nine groups treated with 21% fructose water for 8 weeks to induce insulin resistance and hyperglycemia. After 8 weeks, oral glucose tolerance test (OGTT) was performed to confirm the insulin resistance (IR). Then, we treated rats with different doses of American ginseng and combined with swimming exercise for 28 days (low-dose, low-dose and exercise, medium-dose, medium-dose and exercise, high-dose, high-dose and exercise, placebo, placebo and exercise, metformin). Second time OGTT was performed to know American ginseng and swimming effects.

RESULTS: In our study, we found that fasting blood glucose was significantly increased in fructose-fed groups. After 8 weeks, the insulin resistance was markedly increased in fructose groups compare to control group (p<.05). Calculated glucose area under curve (GAUC) was significantly decreased in both high-dose of ginseng and medium-dose of ginseng combined with exercise compared to placebo group. The HOMA (homeostasis model assessment of IR), an indicator of insulin sensitivity, was significantly increased in placebo, low-dose and medium-dose groups (p<.05) compared to control group. This elevation was attenuated by exercise in placebo and low-dose groups. High-dose intake of ginseng also decreased the HOMA compare to placebo (p<.05) group.

CONCLUSIONS: Our study concludes that high-dose of American ginseng can control the blood glucose levels in hyperglycemia rats. Furthermore, medium-dose of American ginseng combined with swimming exercise also able control the blood glucose in hyperglycemia rats.

1372 Board #153 MAY 30 11:00 AM - 12:30 PM

Panax ginseng Extracts Suppress Hepatic Oxidative Damage in Exhaustive Exercised Rats

Ming-Fen Hsu1, Hsin-Yi Hsu2, Szu-Hsien Yu1, Mallikarjuna Korivi1. 1Taipei Physical Education College, Taipei, Taiwan. 2Aletheia University, Taipei, Taiwan. (Sponsor: Chia-Hua Kuo, FACSM)

(No relationships reported)

PURPOSE: Chinese herbal medicine is most popular in the world, and ginseng is widely used herb in that traditional medicine. Previous studies showed divergent results from ginseng extracts. This study was aimed to examine the effects of purified ginseng extract on oxidative damage in liver of rats.

METHODS: In the present study, 80 male Sprague Dawley (SD) rats were equally divided into four groups, including placebo (0.9 % saline), DS-20 (Dammarane oligo-saponins 20 mg/kg), DS-60 (60 mg/kg) and DS-120 (120 mg/kg bodyweight) groups. DS was orally administered for a period of 10 weeks. After completion of the last treatment half number of rats (n=10) from each group performed exhaustive swimming exercise.

RESULTS:A significant (p<0.05) decrease in hepatic glutathione peroxidase (GSH-Px) activity after exhaustive exercise in placebo group was ameliorated in DS pretreated rats. Glutathione S-transferase (GST) and glutathione reductase (GR) activities were not significantly altered after exhaustive exercise in placebo. Interestingly, DS pretreatment for 10 weeks significantly increased GST activity. The activity of free radical sourse enzyme, xanthine oxidase (XO) was significantly elevated after exhaustive swimming and abolished in DS pretreated rats. In addition, the lipid peroxidation marker, thiobarbituric acid reactive substance (TBARS) was dramaticly raised after exhaustive performance in placebo group and attenuated in DS pre-treated group.

CONCLUSIONS: Administration of Panax ginseng extract, Dammarane oligo-saponins can protect liver from oxidative damage by suppressing free radical production and increasing antioxidant status after exhaustive exercise.

1373 Board #154 MAY 30 11:00 AM - 12:30 PM

Dose Dependant Studies of Panagin DS-1227 on Free Radical Scavenging System in the Liver of Exhaustive Exercise Rats

Mallikarjuna Korivi1, Hsin-Yi Hsu2, Ming-Fen Hsu1, Szu-Hsien Yu1, Feng-Chih Hsu3, Chih-Yang Huang4. 1Taipei Physical Education College, Taipei, Taiwan. 2Aletheia Universtity, Taipei, Taiwan. 3National Taoyuan Agricultural & Industrial Vocational High School, Taoyuan, Taiwan. 4China Medical University, Taichung, Taiwan. (Sponsor: Kuo Chia-Hua, FACSM)

(No relationships reported)

PURPOSE: Ginseng extracts are able to cope against oxidative stress condition; however it is limited to emphasize the therapeutic application of specific compounds with specific dose. This study was designed to explore the therapeutic properties of Panagin DS-1227 (HPLC-grade extract of ginseng stem and leaves), against exhaustive exercise-induced oxidative stress in rats.

METHODS: Rats (n=120) were evenly divided into four groups such as, control, P-20, P-60 and P-120. Panagin DS-1227 (P) extracts were orally administered to respective groups at 20 (low), 60 (medium) and 120 (high) mg/kg bodyweight for 10-week. Half number of rats from each group performed exhaustive exercise in a swimming pool and sacrificed along with their unexercised rats immediately after exercise.

RESULTS: Exhaustive exercise-induced drastic (P<0.001) drop in liver glutathione (GSH) content was significantly restored in P-20 and P-60 groups, while unable to restore in DS-120 group. Oxidative damage to proteins was evidenced by increased protein carbonyl (PC) levels in control exercised rats. Interestingly, low dose was marginally, and medium dose was completely decreased the PC levels, whereas, no change with high dose of Panagin. In addition, high dose significantly increased the PC levels at resting condition. Nitric oxide (NO) levels were increased in control group after exhaustive exercise, nevertheless, no change with all doses of Panagin. Superoxide dismutase (SOD) and catalase (CAT) activities were significantly decreased as a result of exercise in control group, and also with high dose of Panagin treatment at resting condition. In contrast, glutathione S-transferase (GST) activity was increased with all doses of Panagin.

CONCLUSIONS: Our findings elucidate that purified ginseng extract, Panagin DS-1227 attenuates the oxidative damage in liver of exhaustive exercised rats. Interestingly, low and medium doses of Panagin showed better results than the high dose.

1374 Board #155 MAY 30 11:00 AM - 12:30 PM

Effect of Dammarane Oligo-Saponins on Selected Biochemical Parameters in Blood of Rats after Exhaustive Exercise

Yi-Ting Lin1, Szu-Hsien Yu1, Ming-Fen Hsu1, Feng-Chih Hsu2, Mallikarjuna Korivi1. 1Taipei Physical Education College, Taipei, Taiwan. 2National Taoyuan Agricultural & Industrial Vocational High School, Taoyuan, Taiwan. (Sponsor: Chia-Hua Kuo, FACSM)

(No relationships reported)

PURPOSE: Therapeutic applications of ginseng extracts are controversial due to inconsistency in ginsenoside profile. In this study we investigated the beneficial effects of Dammarane Oligo-Saponins (DS) and their dose response on selected biochemical parameters in the blood of exhaustive exercised rats.

METHODS: Total sixty Sprague Dawley rats were evenly divided into four groups, including control, DS-20, DS-60 and DS-120. DS extract was orally administered to respective groups at the dose of 20, 60, 120 mg/kg bodyweight for a period of 8-week. All the rats were performed exhaustive swimming exercise after the last treatment. Blood samples were collected from the tail vein before exercise, immediately, 1-h and 3-h after exercise.

RESULTS: Recorded average swimming duration seems higher in DS treated groups, but statistically not reached the significant level. Baseline blood glucose, lactate, glutamic-oxaloacetic transaminases (GOT), blood urea nitrogen (BUN) and creatine kinase (CK) were not significantly different among groups. Blood glucose levels were significantly (P<0.05) higher in DS-60 and DS-120 groups compared to control group immediately after exercise, and 1-h after exercise. Contrary to other doses, high dose (DS-120) group showed significantly lower GOT levels 1-h after exercise. BUN levels were significantly lower 3-h after exercise in DS-60 group compared to control group at same time point. Interesting finding of the present study is that increased CK levels after exercise were decreased in all DS treated groups 3-h after exercise compared to control group.

CONCLUSIONS: The data of the present study concludes that purified ginseng extracts, Dammarane Oligo-Saponins possesses beneficial effects against exhaustive exercise challenge. Further detailed investigations are under progress to suggest the DS as nutraceutical substance.

1375 Board #156 MAY 30 11:00 AM - 12:30 PM

Coffee Reduces Liver Damage through Increased SOD Activity in Liver of NASH Rats

Yi-Tse Wang1, Che-Chang Chan2, Shan-Ken Hung1, Shiow-Chwen Tsai1. 1Taipei Physical Education College, Taipei, Taiwan. 2Division of Gastroenterology, Department of Internal Medicine, Taipei Veterans General Hospital, Shih-Pai, Taipei 11217, Taiwan, R.O.C., Taipei, Taiwan. (Sponsor: Chia-Hua Kuo, FACSM)

(No relationships reported)

PURPOSE: Non-alcoholic fatty liver diseases (NAFLD) are wide spectrum diseases ranging from simple steatosis, non-alcoholic steatohepatitis (NASH) to liver cirrhosis, and induce hepatocellular carcinoma. Coffee has been reported to reduce the risk of advanced liver disease and its complications as well as hepatocellular carcinoma.To investigate whether coffee improved MCD diet induced steatosis and oxidative stress in liver.

METHODS: In this study, 8-week-old male Sprague Dawley rats were randomly divided into 5 groups: control (fed normal diet, W0), methionine-choline-deficient (MCD) diet fed for 9 weeks or 14 weeks combined fed with water (W9, W14) or fed with coffee (W9+Co, W14+Co). Coffee (corresponding to approximately 6 cups of espresso coffee or 2 cups of filtered coffee for a person weighing 70 kg) wase fed daily for 4 weeks to the rats who were being fed a MCD diet for the previous 5 or 10 weeks. The blood were collected and analyzed for AST. The liver tissues were collected, extracted and assayed for the production of glutathione (GSH) and lipid peroxidation and the activities of antioxidant enzymes by using commercial kits. The hematoxylin-eosin staining was used to determine the degree of lobular inflammation and fibrosis in liver. The degree of fibrosis and inflammation were evaluated by METAVIR score. The statistical analysis was done using the one-way ANOVA for independent samples, with significance level of 5%.

RESULTS: Rat fed with the MCD diet showed a rapid induction of AST and hepatic steatosis, loss of body weight and liver weight, but increased the ratio of liver and body weight. Coffee significantly decreased steatosis of W9 (p<0.05) and fibrosis of W14 (p<0.05). The production of lipid peroxidation in liver increased significantly in W14 (24.0±6.9 vs 0.40±0.05 nmole/min/μg protein, p<0.05) but not in W14+Co compared with W0 group. The activity of superoxide dismutase (SOD) in W14 and W14 +Co were increased compared with W0. Feeding with MCD diet for 14 weeks significantly decreased the activities of catalase (p<0.01), glutathione peroxidase (GPx) (p<0.05), and glutathione reductase (GR) (p<0.05). Administration of coffee did not alter MCD diet abolished activities of antioxidant enzymes.

CONCLUSIONS: Administration of coffee improves NAFLD through increased activity of SOD in liver.

1376 Board #157 MAY 30 11:00 AM - 12:30 PM

Baker’S Yeast Beta Glucan Supplementation Reduces The Number Of Cold/Flu Symptomatic Days After Completing A Marathon

James A. Navalta1, Katie C. Carpenter2, Whitney L. Breslin2, Tiffany Davidson2, Brian K. McFarlin, FACSM2. 1Western Kentucky University, Bowling Green, KY. 2University of Houston, Houston, TX. (Sponsor: Brian K McFarlin, FACSM)

(No relationships reported)

PURPOSE: Marathon running places a profound stress on one’s body. Such stress manifests itself in the form of muscle soreness, fatigue, and a weakened immune system. It is common for marathon runners to develop an upper respiratory tract infection in the days and weeks following completion of a marathon. The present study sought to examine a commercially available form of Baker’s yeast β-glucan (BG); this form of BG has been previously demonstrated to boost immune system function in marathon runners and in laboratory studies where subjects completed a defined exercise stimulus.

METHODS: We recruited 324 subjects who were completing in the 2011 Austin Livestrong Marathon (Austin, TX). Upon enrolling in the study, subjects completed a demographics question designed to provide information about their exercise training patterns and health status. Subjects were also provided either a BG (250 mg/d) or placebo (sugar pill, PL) supplement. Double-blind administration of the supplement was used to reduce bias. Subjects were also given 2 packets of surveys that were returned at 2 and 4 weeks post marathon. Of the 324 enrolled, only 182 subjects completed and returned both sets of surveys. The set of surveys included were the Profile of Mood States (POMS), the Wisconsin Upper Respiratory Tract Symptom Survey (WURSS), and a daily health/exercise log. Survey data was entered into a database using a custom scanning solution. Data were analyzed for significance using separate repeated measures ANOVAs with a P<0.05.

RESULTS: BG supplementation significantly reduced both the number of days that subjects reported both general health problems as well as cold/flu symptoms. We did not find any significant differences in either POMS or WURSS scores between groups.

CONCLUSIONS: The key finding of the present study was that BG supplementation post-marathon reduced the number of symptomatic days experienced by a subject. Based on previous studies from our lab and others, it is reasonable to speculate that the improvements associated with BG were likely due to alterations in monocytes, plasma cytokines, and improved mucosal immunity. This study was funded by Biothera, The Immune Health Company.

1377 Board #158 MAY 30 11:00 AM - 12:30 PM

Supplementation With Baker’S Yeast Beta Glucan Improves Mucosal Immunoglobulin Profile After Exercise In A Hot, Humid Environment

Brian K. McFarlin, FACSM, Whitney L. Breslin, Katie C. Carpenter, Tiffany Davidson, Amy Adams. University of Houston, Houston, TX.

(No relationships reported)

PURPOSE: Strenuous exercise is known to suppress mucosal immunity for up to 24-h, which can increase the risk of developing an upper respiratory tract infection. While many dietary interventions have been used to combat post-exercise immune suppression, most have been ineffective. Recent evidence has suggested that a commercially-available form of baker’s yeast β-glucan may be useful as an immune-booster. The purpose of this study was to determine if 10-d of supplementation with baker’s yeast β-glucan (BG) prior to a bout of exercise in a hot (37±2°C), humid (45±5% relative humidity) environment improves mucosal immunity during recovery from exercise in recreationally active subjects (29 men, 31 women, 22±4 y).

METHODS: Subjects completed 49±6 min of cycling after consuming either BG (250 mg/d) or a placebo (sugar pill, PL) for 10-d prior to each exercise session. The investigators were blinded to the supplement conditions until all data was collected and analyzed. Saliva was collected using a salivette placed under the tongue at baseline (BASE), before exercise (PRE), immediately after (POST), and two-hours after (2H) exercise. The salivette was kept in the mouth for 10-min and then transferred to a specially designed freezer tube and frozen (-80°C) until analysis for salivary IgA, IgM, IgG1, and IgG2 using a multiplex kit (MagPix). Data were analyzed using separate repeated measures ANOVAs and significance was set at P<0.05.

RESULTS: BG supplementation was associated with an increase in salivary IgA (P=0.048) and a decrease in salivary IgM (P=0.029) at 2H compared to placebo. In the placebo condition, there was a progressive decline in IgA and an increase in IgM, with the most pronounced changes occurring at 2H. Also, in the BG condition, IgA was increased and IgM was decreased at 2H compared to PRE and the placebo 2H samples. We did not find any significant differences for either salivary IgG1 or IgG2.

CONCLUSIONS: These findings suggest that supplementation with baker’s yeast β-glucan may improve mucosal immunity following a strenuous bout of exercise. While previous research is not conclusive, a boost to mucosal immunity may reduce susceptibility to upper respiratory tract infection. This study was funded by Biothera, The Immune Health Company.

1378 Board #159 MAY 30 11:00 AM - 12:30 PM

Baker’S Yeast β-glucan Supplementation Improves Monocyte And Cytokine Responses Following Exercise In A Hot, Humid Environment

Katie C. Carpenter, Whitney L. Breslin, Tiffany Davidson, Amy Adams, Brian K. McFarlin, FACSM. University of Houston, Houston, TX. (Sponsor: Brian K McFarlin, FACSM)

(No relationships reported)

PURPOSE: Strenuous exercise is known to suppress the immune system, which can increase the chances of getting sick in the hours after exercise. The purpose of this study was to determine if 10-d of supplementation with yeast β-glucan alters monocyte concentration, LPS-stimulated cytokine production, and plasma cytokine concentration in recreationally active subjects.

METHODS: Recreationally active subjects (29 men, 31 women, 22±4 y) completed 49±6 min of cycling (37±2°C, 45±5% relative humidity) after consuming either yeast β-glucan (250 mg/d, BG) or a placebo (sugar pill, PL) for 10-days prior to each exercise session. The investigators were blinded to the supplement conditions until all data was collected and analyzed. Venous blood was collected at baseline (prior to supplement), pre-, post-, and 2-hours (2H) post exercise. Total and subset monocyte concentration was measured by flow cytometry. LPS-stimulated production of 12 cytokines was measured using a whole blood assay. Plasma concentration of 13 cytokines was measured using a high-sensitivity MagPix assay.

RESULTS: Monocyte (CD14+) concentration was significantly greater at 2H (P=0.05) with BG. Also, compared to PL, BG boosted LPS-stimulated production IL-2, IL-4, IL-5, and IFN-γ at PRE and POST (P<0.05). Plasma concentration of IL-2, IL-4, IL-5, IL-7, IL-10, and IFN-γ were significantly greater at 2H in the BG compared to PL. In the placebo condition we observed the traditional response to strenuous exercise (rise at POST and suppression at 2H). It appears that 10-days of supplementation with BG primed blood leukocytes for the production of IL-2, IL-4, IL-5, and IFN-γ. These cytokines were elevated prior to and immediately after exercise in LPS-stimulated cultures and subsequent elevation were observed at 2H with unstimulated plasma measures. In addition to cytokine changes, BG appeared to blunt post-exercise reduction in blood monocyte concentration, which may have implication of immune-surveillance.

CONCLUSIONS: The key findings of the present study demonstrate that BG may be a suitable countermeasure to protect and boost the immune system following stressful exercise. Such boost is likely to lower the duration of the “open window” response. This study was funded by Biothera, The Immune Health Company.

1379 Board #160 MAY 30 11:00 AM - 12:30 PM

Effect of Six Weeks of Oral Echinacea Purpurea Supplementation on Erythropoiesis

Tyler D. Martin1, Michael S. Green1, Malcolm T. Whitehead2, Timothy P. Scheett3, Michael J. Webster, FACSM4. 1Troy University, Troy, AL. 2Arkansas State University, State University, AR. 3College of Charleston, Charleston, SC. 4The University of Southern Mississippi, Hattiesburg, MS.

(No relationships reported)

Echinacea purpurea, a purple coneflower plant of the compositae family (Asteraceae), is native to North America and commonly used as an herbal supplement to enhance immune function. Recent research has demonstrated that four weeks of oral Echinacea purpurea supplementation (8,000 mg·d-1) in untrained males (42.5 ± 1.6 mL·kg-1·min-1) significantly increased serum erythropoietin (EPO). The increase in EPO was not accompanied by a significant increase in the number of red blood cells (RBCs) or hemoglobin concentration [Hb]; however, there were non-significant increases in the erythropoietic status by the end of the four week study (% change in RBC, hematocrit (Hct), [Hb], mean corpuscular volume (MCV)), suggesting the initiation of erythropoiesis.

PURPOSE: To investigate the effect of six weeks of oral Echinacea purpurea supplementation on serum EPO and erythropoietic status.

METHODS: Twenty-four males (mean ± SE): age = 25.2 ± 1.4 yr, height = 178.1 ± 1.4 cm, mass = 78.1 ± 1.6 kg, percent body fat = 12.7 ± 0.9 %, VO2 max = 52.9 ± 0.9 mL·kg-1·min-1 were randomly grouped using a matched-pair, double-blind design and self-administered 8,000 mg·d-1 (5 × 400 mg × 4 times·d-1) of either Echinacea purpurea (ECH) (n=12) or placebo (PLA) (n=12) for 42 consecutive days. Blood samples were collected and analyzed for EPO, RBCs, Hb, Hct, MCV, and mean corpuscular hemoglobin concentration (MCHC). Separate 2 × 4 (Group × Time) factorial ANOVA with repeated measures were used to determine statistical differences with significance set at p ≤ 0.05.

RESULTS: There were no statistically significant (p > 0.05) interaction, group or time effects observed for EPO or erythropoietic status markers.

CONCLUSION: Six weeks of oral ECH supplementation in apparently healthy, recreationally active, males with above average fitness status (VO2max = 52.9 ± 0.9 mL·kg-1·min-1), does not enhance EPO or erythropoietic status. These findings are in contrast with previous reports of Echinacea supplementation. Any explanation for these differences, including the role that training and/or physical fitness level of participants, is unclear and speculative.

Supported by Troy University Faculty Development Research Grant.

1380 Board #161 MAY 30 11:00 AM - 12:30 PM

Effects of 16wk Fucoxanthin and Punicic Acid Supplementation I: Body Composition and Hemodynamic Changes

Geoffrey M. Hudson, Lisa A. Knecht, Cody J. Tullos, Emily R. Buras, Bethany L. Boleware, James T. Goetz, David E. Krzeminski, Alicia D. Sample, Michael J. Webster, FACSM. The University of Southern Mississippi, Hattiesburg, MS.

(No relationships reported)

Supplementation with 100 mg brown seaweed extract (0.8 % fucoxanthin) and 100 mg pomegranate seed oil (70 % punicic acid), abbreviated as Xan, has been shown to significantly reduce body fat, liver fat, and blood pressure (BP) in obese females.

PURPOSE: To determine the effects of Xan supplementation on body composition, heart rate, and BP in obese men and women.

METHODS: Twenty-nine obese men and women (29±8 y; 36.50±5.39 kg/m2; 43.8±8.0 % body fat) were matched on gender, age, and body fat percentage and randomized to either a Xan (n=14) or olive oil placebo (Pla; n=15) group. This study was double-blind and placebo-controlled. Participants were instructed to ingest 200 mg capsules of their given supplement three times per day (prior to meals) for 16 weeks while consuming a reduced calorie diet (equivalent to their resting energy expenditure). The 2011 ACSM physical activity guidelines were also recommended. Data were analyzed with repeated measures ANOVA and presented as means ± SD changes from baseline.

RESULTS: Analysis of the data demonstrated that body mass (Xan:-1.83±3.48 kg; Pla:-3.17±3.42 kg) and body mass index (Xan:-0.64±1.19 kg/m2; Pla:-1.14±1.21 kg/m2) were significantly reduced over the 16 weeks (p=0.001; p=0.001) with no group differences. Diastolic BP was significantly reduced (Xan:-6±9 mmHg; Pla:-2±9 mmHg; p=0.021), while there was a trend for a reduction of systolic BP (Xan:-10±10 mmHg; Pla:-3±13 mmHg; p=0.059). No significant within- or between-group effects were observed in body fat percentage or lean body mass.

CONCLUSION: The 16 weeks of Xan supplementation (600 mg/d) did not augment the effects of exercise and a reduced calorie diet on weight and BP in this obese population.

Supported by a grant from P.L. Thomas & Co., Inc.

1381 Board #162 MAY 30 11:00 AM - 12:30 PM

Effects of 16wk Fucoxanthin and Punicic Acid Supplementation II: Metabolism

Bethany L. Boleware, Lisa A. Knecht, Geoffrey M. Hudson, Cody J. Tullos, Emily R. Buras, David E. Krzeminski, James T. Goetz, Alicia D. Sample, Michael J. Webster, FACSM. The University of Southern Mississippi, Hattiesburg, MS.

(No relationships reported)

Supplementation with 100 mg brown seaweed extract (0.8 % fucoxanthin) and 100 mg pomegranate seed oil (70 % punicic acid), abbreviated as Xan, has been shown to significantly reduce body fat and liver fat assumedly by increasing metabolism.

PURPOSE: To determine the effects of Xan supplementation on resting energy expenditure (REE) and respiratory quotient (RQ) in obese men and women.

METHODS: Twenty-nine obese men and women (29±8 y; 36.50±5.39 kg/m2; 43.8±8.0 % body fat) were matched on gender, age, and body fat percentage and randomized to either a Xan (n=14) or olive oil placebo (Pla; n=15) group. This study was double-blind and placebo-controlled. Participants were instructed to ingest 200 mg capsules of their given supplement three times per day (prior to meals) for 16 weeks while consuming a reduced calorie diet (equivalent to their resting energy expenditure). The 2011 ACSM physical activity guidelines were also recommended. Data were analyzed with repeated measures ANOVA and presented as means ± SD changes from baseline.

RESULTS: Analysis of the data demonstrated significant reductions in REE (Xan:-38±140 kcal·d-1; Pla:-59±153 kcal·d-1) and absolute VO2 (Xan:-0.003±0.021 L·min-1; Pla:-0.008±0.021 L·min-1) over the 16 weeks (p=0.007; p=0.001) with no between-group effects. No within- or between-group effects were observed with relative VO2, VCO2, or RQ.

CONCLUSION: As expected, weight loss resulted in a reduced REE; however, 16 weeks of Xan supplementation (600 mg/d) did not prevent reductions in REE typically occurring with weight loss.

Supported by a grant from P.L. Thomas & Co., Inc.

1382 Board #163 MAY 30 11:00 AM - 12:30 PM

Effects of 16wk Fucoxanthin and Punicic Acid Supplementation III: Serum Lipids Changes

Cody J. Tullos, Lisa A. Knecht, Geoffrey M. Hudson, Bethany L. Boleware, Emily R. Buras, Alicia D. Sample, Michael J. Webster, FACSM. The University of Southern Mississippi, Hattiesburg, MS.

(No relationships reported)

Supplementation with 100 mg brown seaweed extract (0.8 % fucoxanthin) and 100 mg pomegranate seed oil (70 % punicic acid), abbreviated as Xan, has been shown to significantly reduce body fat, liver fat, and serum lipids in obese females.

PURPOSE: To determine the effects of Xan supplementation on serum lipid levels [i.e. high-density lipoprotein (HDL), low-density lipoprotein (LDL), total cholesterol, triglycerides] in obese men and women.

METHODS: Twenty-nine obese men and women (29±8 y; 36.50±5.39 kg/m2; 43.8±8.0 % body fat) were matched on gender, age, and body fat percentage and randomized to either a Xan (n=14) or olive oil placebo (Pla; n=15) group. This study was double-blind and placebo-controlled. Participants were instructed to ingest 200 mg capsules of their given supplement three times per day (prior to meals) for 16 weeks while consuming a reduced calorie diet (equivalent to their resting energy expenditure). The 2011 ACSM physical activity guidelines were also recommended. Data were analyzed with repeated measures ANOVA and presented as means ± SD changes from baseline.

RESULTS: Data analysis demonstrated that there was a trend for a reduction in serum triglycerides (Xan:-31±45 mg·dl-1; Pla:-5±66 mg·dl-1; p=0.071) and a trend for an interaction effect (p=0.088). No significant within- or between-group effects were observed in serum total cholesterol, HDL, or LDL levels.

CONCLUSION: The 16 weeks of Xan supplementation (600 mg/d) did not significantly augment the beneficial effects of exercise and a reduced calorie diet on serum cholesterol and triglyceride levels in this obese population. However, a larger decrease in serum triglyceride levels was observed in the Xan group, which may be of clinical significance. Supported by a grant from P.L. Thomas & Co., Inc.

1383 Board #164 MAY 30 11:00 AM - 12:30 PM

Effects of 16wk Fucoxanthin and Punicic Acid Supplementation IV: Markers of Liver and Kidney Function

Emily R. Buras, Geoffrey M. Hudson, Lisa A. Knecht, Bethany L. Boleware, Cody J. Tullos, Alicia D. Sample, Michael J. Webster, FACSM. The University of Southern Mississippi, Hattiesburg, MS.

(No relationships reported)

Supplementation with 100 mg brown seaweed extract (0.8 % fucoxanthin) and 100 mg pomegranate seed oil (70 % punicic acid), abbreviated as Xan, has been shown to significantly reduce body fat, liver fat, and improve serum markers of liver function in obese females.

PURPOSE: To determine the effects of Xan supplementation on serum markers of liver and kidney function in obese men and women.

METHODS: Twenty-nine obese men and women (29±8 y; 36.50±5.39 kg/m2; 43.8±8.0 % body fat) were matched on gender, age, and body fat percentage and randomized to either a Xan (n=14) or olive oil placebo (Pla; n=15) group. This study was double-blind and placebo-controlled. Participants were instructed to ingest 200 mg capsules of their given supplement three times per day for 16 weeks while consuming a reduced calorie diet (equivalent to their resting energy expenditure). The 2011 ACSM physical activity guidelines were also recommended. Data were analyzed with repeated measures ANOVA and presented as means ± SD changes from baseline.

RESULTS: Data analysis demonstrated that significant reductions in serum levels of alkaline phosphatase (ALP; Xan:-5±6 U·L-1; Pla:-3±9 U·L-1; p=0.001), alanine aminotransferase (ALT; Xan:-12±22 U·L-1; Pla:-4±7 U·L-1; p=0.021), total protein (Xan:-0.3±0.3 g·dl-1; Pla:-0.2±0.3 g·dl-1; p<0.001), and creatinine (Xan:-0.11±0.15 mg·dl-1; Pla: X:-0.08±0.13 mg·dl-1; p<0.001) were observed over the 16 weeks with a trend for an interaction effect for ALT (p=0.101), but no other between-group effects. Blood urea nitrogen (BUN) levels did not change over time, but there was a significant between-group effect (Xan:14±4 mg·dl-1; Pla:11±3 mg·dl-1; p=0.018). No within- or between-group effects were observed in serum aspartate aminotransferase, total bilirubin, or albumin.

CONCLUSION: The 16 weeks of Xan supplementation (600 mg/d) did not affect these liver and kidney function markers in this obese population.

Supported by a grant from P.L. Thomas & Co., Inc.

1384 Board #165 MAY 30 11:00 AM - 12:30 PM

The Effect of Yoga Exercise and Ascorbic acid Supplementation on Stress Hormones in Korean Young Females

Malryun Shin1, Jungho Cho1, Ikwon Kang2, Jihyun Lee1, Jaehyun Jung1, Sunmin Kim1, Myungjoo Yang1, Sooyoun Kim1. 1Sookmyung Women’s University, Seoul, Korea, Republic of. 2Hongik University, Seoul, Korea, Republic of.

(No relationships reported)

PURPOSE: The purpose of this study was to investigate the effects of 8 weeks of ascorbic acid supplementation and yoga exercise on epinephrine, norepinephrine, glucagon and cortisol.

METHODS: Thirty young females were randomly assig±ned to one of three group, I.e., Group I(n=10): placebo(1000mg/day); GroupII(n=10): ascorbic acid(500mg/day) and placebo(500mg/day); Group III(n=10): ascorbic acid(1000mg/day) with yoga exercise(55-75% of HRmax, 3-4 d/w, 60-90min/d). The following measurements were made on all subjects before and after 8 week of experiment. Analysis of covariance and bonferroni test were used to determine the statistical significance.

RESULTS: There were no significant differences in epinephrine, norepinephrine and glucagon but cortisol were significantly changed(p<.05).

CONCLUSIONS: 8 week’s ascorbic acid supplementation with yoga exercise was effective for epinephrine, norepinephrine, glucagon and cortisol in Korean young females. Determination of which exercise types, ascorbic acid dosage and fitness status of individuals would produce reproducible ergogenic effects is a logical extension of current research.

1385 Board #166 MAY 30 11:00 AM - 12:30 PM

Vitamin C Does Not Prevent Endurance Training-induced Improvement Of Glucose Tolerance And Insulin Sensitivity

Ayuko Nii, Koichi Yada, Shigeru Obara, Hideki Matoba. The University of Tokushima, Tokushima, Japan.

(No relationships reported)

Conflicting results have been reported concerning the involvement of reactive oxygen species (ROS) in endurance training-induced improvement of glucose tolerance and insulin sensitivity.

PURPOSE: The purpose of this study was to determine whether vitamin C supple-mentation prevented the training-induced improvement of glucose tolerance and insulin sensitivity in rats.

METHODS: Twenty male rats,4-week of age,were assigned to four groups:sedentary control,sedentary with vitamin C supplementation,trained control,trained with vitamin C supplementation (n=5 each).The rats of vitamin C supplemented groups were ad-ministered 500mg per kg body weight of vitamin C from 12 days before the start of the training until the end of the training period.The rats of the trained groups swam 6h/day with two 3h sessions separated by 45 min of rest.The training lasted for 2 weeks with a frequency of 5 days per week.After the training period, the rats underwent intraperitoneal glucose tolerance test (IPGTT).Glucose and insulin responses during IPGTT were assessed by the area under the curve (AUC).Insulin resistance was eva-luated using homeostasis model assessment as an index of insulin resistance (HOMA-R).Insulin sensitivity was evaluated using composite whole-body insulin sensitivity index (ISI (comp)).

RESULTS: The training significantly suppressed body weight gain (P<0.01).There was no difference in fasting glucose levels and fasting insulin levels among the groups.There was also no significant difference in the HOMA-R indices among the groups.On the other hand,the training significantly lowered the AUC for glucose and insulin (P<0.05).The training significantly increased ISI (comp)(P<0.05).The vitamin C supplementation did not alter the AUC for glucose,the AUC for insulin and ISI (comp).

CUNCLUSION: This study suggests that the vitamin C supplementation does not prevent the training-induced improvement of glucose tolerance and insulin sensitivity in rats.

1386 Board #167 MAY 30 11:00 AM - 12:30 PM

Effects of Quercetin Supplementation on Physical Function in Older Adults

Stephen C. Chen1, J. Mark Davis, FACSM1, E. Angela Murphy1, Michele Neese2, Matthew Kostek1, Kei Lam1, John Sieverdes1, Victor Hirth2, Andres Leone2, Seung H. Jung1, Benjamin Gordon1, Katie Becofsky1, Toni Torres-McGehee1, Steve Blair, FACSM1, J. Larry Durstine, FACSM1. 1University of South Carolina, Columbia, SC. 2Palmetto Health Senior Primary Care, Columbia, SC. (Sponsor: Mark Davis, FACSM)

(No relationships reported)

Fatigue is a common complaint among older adults and is associated with functional decline and multiple adverse health outcomes. Previous studies in our laboratory have shown that the dietary flavonoid quercetin can enhance voluntary activity and endurance performance in mice as well as aerobic capacity in young adults. However, the benefits of quercetin on physical function in older adults has not yet been evaluated.

PURPOSE: To determine the effects of short-term quercetin (QUE) versus placebo (PLA) supplementation on habitual physical activity (N=10), physical performance (N=18) as well as markers of mitochondrial biogenesis (N=4) in healthy older adults (61 to 89 years).

METHODS: Subjects were randomized to receive QUE (1000 mg·day-1) or PLA for 14 days using a double-blind, placebo controlled, crossover study design with a 2-week washout period. Physical activity patterns were assessed by accelerometry daily throughout the study. After completion of treatments, physical performance including VO2 peak, Short Physical Performance Battery (SPPB) and 6-minute walk (6MW) were determined, and muscle biopsies acquired from the vastus lateralis were analyzed for mRNA gene expression of PGC-1α, citrate synthase and cytochrome c using RT-PCR.

RESULTS: QUE feedings significantly increased daily steps count by 16.7% compared with PLA (4811.7 ± 778.5 vs. 5614.9 ± 993.3 steps·day-1, respectively, P=0.02), along with a trend for increased minutes of moderate intensity of physical activity (10.5 ± 3.9 vs. 7.6 ± 2.6 min·day-1, P=0.11). QUE increased mRNA gene expression of PGC-1α, citrate synthase and cytochrome c by 49%, 26% and 32%, respectively, although these did not reach statistical significance which is likely due to the small sample size that was used in this analysis. No differences were found between QUE and PLA conditions for VO2 peak, SPPB score, and 6MW distance.

CONCLUSION: These novel data suggest that short-duration QUE feedings can promote physical activity in older adults, and that these effects may be partially attributed to the increased muscle mitochondrial biogenesis which plays a role in fatigue development. Overall, the results of this study provide evidence in support of dietary QUE as a possible safe and effective nutritional strategy for disease prevention and health promotion in the elderly.

1387 Board #168 MAY 30 11:00 AM - 12:30 PM

Effect Of Resveratrol On Oxidative Stress And Skeletal Muscle Oxygenation With NIRS In Trained Cyclists

Claude Lajoie, Sana Driss, Maria-Grazia Martinoli, Simon Bergeron-Vaillancourt, Louis Laurencelle, Fanny Longpré. UQTR, Trois-Rivieres, QC, Canada. (Sponsor: Francois Trudeau, FACSM)

(No relationships reported)

During strenuous exercise, there is a dramatic increase in oxygen consumption that leads to an increased oxygen species (ROS) production. This increase in ROS results in oxidative stress, which has been associated with muscle fatigue and impaired recovery following high-intensity aerobic exercise.

PURPOSE: The purpose of the present study was to evaluate the effects of a Resveratrol supplementation on protein blood markers of oxidative stress and blood antioxidant capacity during a single bout of intermittent exercise and recovery in trained cyclists. A second purpose was to measure vastus lateralis muscle oxygenation, using near infrared spectroscopy (NIRS), blood lactate concentration and whole body oxygen consumption under the Resveratrol condition.

METHODS: A double-blind study was conducted with 7 male trained cyclists, aged 37.3 11.7 years. The participants completed two sessions high intensity interval training (HIT) in two conditions, with and without Resveratrol supplementation (1000 mg daily) in orange juice, 7 consecutive days before the experiment, with partially counterbalanced conditions across subgroups. Blood sampling was collected pre- and post-exercise for the determination of protein carbonyls (PC) and total antioxidant capacity (TAC).

RESULTS: Resveratrol supplementation did not improve organism’s antioxidant defence and protein carbonyl blood concentrations following HIT. Under Resveratrol supplementation, tissue saturation index in oxygen (TSI%) in vastus lateralis was slightly less attenuated (p<0.05) at the end of HIT (Δ = -1.3%) as compared to placebo condition (Δ = -2.0%). Resveratrol had no effect on skeletal muscle oxyhemoglobin (O2Hb) deoxyhemoglobin (HHb) and total hemoglobin (THb). Heart rate and blood lactate concentration recovery both showed a tendency (p 0.10) to decrease following 15 min passive recovery under the Resveratrol condition. However, a positive correlation trend (r = 0.72, p = 0.07) was

observed between THb and TAC.

CONCLUSION: This study did not show an effect of Resveratrol on protein carbonyl and antioxidant capacity. However, Resveratrol improved tissue saturation index (TSI%) in vastus lateralis during the HIT.

1388 Board #169 MAY 30 11:00 AM - 12:30 PM

Involvement of PI3K/Akt/mTOR Pathway in Angelica sinensis Induced Myotube Hypertrophy.

Tzu-Shao Yeh, FACSM1, Jen-Fang Liu1, Mei-Chich Hsu, FACSM2. 1Taipei Medical University, Taipei, Taiwan. 2National Taiwan Sport University, Taoyuan, Taiwan.

(No relationships reported)

PURPOSE: Herbal medicine has long been used in ergogenic aids for athletes but there is little scientific evidence for their actions. To investigate whether Angelica sinensis increases hypertrophy of myotubes through activating the PI3K/Akt/mTOR pathway, have been proposed to promote skeletal muscle hypertrophy and prevent muscle atrophy.

METHODS: We examined the PI3K/Akt/mTOR pathway in C2C12 myotubes, a well-established in vitro model of skeletal muscle hypertrophy, with or without Angelica sinensis extract for 72 hours.

RESULTS: In Angelica sinensis extract treated, the average myotube diameter was 1.34±0.13 fold to normal, which is significantly larger than the diameter in non-herbal supplements cultures. This indicates clearly that myotubes were hypertrophied by Angelica sinensis extract. Hypertrophy was largely suppressed by wortmannin or rapamycin, inhibitors of PI3K or mTOR, respectively. The PI3K inhibitor, wortmannin, decreased the diameter of Angelica sinensis extract treated myotubes by 25%, as well as that of positive controls by approximately 30%. The mTOR inhibitor, rapamycin behaved similarly to wortmannin. These results indicate that the PI3K/Akt/mTOR pathway plays an important role in Angelica sinensis-induced myotube hypertrophy.

CONCLUSIONS:Angelica sinensis promotes hypertrophy in cultured skeletal myotubes through activating the PI3K/Akt/mTOR pathway. Furthermore, phosphorylation of Akt was enhanced by Angelica sinensis treatment and suppressed by wortmannin. Angelica sinensis and its derivatives may be promising candidates for the treatment of muscular dystrophy.

1389 Board #170 MAY 30 11:00 AM - 12:30 PM

Efficacy of Tart Cherry Juice to Reduce Inflammation Among Patients with Osteoarthritis

Adriana E. Sleigh, Kerry S. Kuehl, Diane L. Elliot, FACSM. Oregon Health & Science University, Portland, OR.

(No relationships reported)

PURPOSE: Osteoarthritis (OA) is a common syndrome affecting 65 million Americans characterized by joint pain, limitation of movement, crepitus, occasional effusion, and variable degrees of local inflammation. Up to 40% of OA patients have inflammation. Numerous antioxidant and anti-inflammatory agents have been identified in tart cherries and may be beneficial for the treatment of pain and inflammation. This study assessed the effects of tart cherry juice on serum inflammation biomarkers among women with inflammatory OA.

METHODS: We used a three week, randomized, placebo controlled design among twenty 40-70 year old females with inflammatory OA. All subjects fulfilled the 1990 American College of Rheumatology classification guidelines for the diagnosis of osteoarthritis with evidence of swelling in at least one joint in the past year. For 21 days, subjects ingested 10.5 fl oz of tart cherry juice or a placebo beverage twice a day. Pre and post drink intervention blood measurements included IL-6, IL-10, TNF-α, and CRP.

RESULTS: Among the subjects who consumed tart cherry juice, all serum biomarkers showed evidence of decreased inflammation, however the only statistically significant change was observed in TNF-α (P<0.05). In addition, a subset analysis was performed on those subjects with active inflammation as defined by CRP>3.0 mg/L. Within the high inflammation subset (n=12), there was a statistically significant decrease in TNF-α and CRP for subjects on the tart cherry juice as compared to placebo.

CONCLUSIONS: Tart cherries have the highest antioxidant and anti-inflammatory content of any food. This study suggests a benefit of tart cherry juice in reducing inflammation as measured by certain serum inflammatory biomarkers among women with OA. Pain relief and improvement of functional disability are the main goals of OA treatment and it is important to look at healthy alternative therapies to conventional methods in the treatment and management of inflammatory osteoarthritis. Tart cherries may provide beneficial anti-inflammatory activity helping OA patients manage their disease.

1390 Board #171 MAY 30 11:00 AM - 12:30 PM

Melatonin And Sleep Quality Are Increased Following Tart Cherry Juice Consumption

Glyn Howatson, FACSM1, Phillip G. Bell1, Jamie Tallent1, Benita Middleton2, Malachy P. McHugh, FACSM3, Jason Ellis1. 1Northumbria University, Newcastle-upon-Tyne, United Kingdom. 2University of Surrey, Guildford, United Kingdom. 3Nicholas Institute of Sports Medicine and Athletic Trauma, NY, NY.

(No relationships reported)

Tart Montmorency cherries contain high levels of phytochemicals that include melatonin. Considering the alleged rampant use of prescription sleep medications in professional sports with a lot of travel (e.g. NHL), cherry juice might be a viable non-pharmacological alternative. We hypothesized that consumption of a tart cherry juice concentrate would provide an exogenous supply of melatonin and have a positive influence on sleep.

PURPOSE: The aim of our investigation was to examine the effect of tart cherry juice on melatonin metabolism and the subsequent influence on sleep quality in healthy asymptomatic adults.

METHODS: Twenty volunteers participated in this randomized, double-blind, placebo controlled, cross-over trial. Cherry juice or a placebo was administered for 7 days following a 48 h baseline period. Measures of sleep quality were determined with actigraphy (sleep onset latency, time in bed, fragmentation index, total sleep time and sleep efficiency) and urinary 6-sulfatoxymelatonin (aMT6); these were recorded for both baseline periods and the final 48 h of each supplement period. Determination of the circadian rhythm (amplitude, acrophase and mesor of aMT6) was determined using cosinor analysis. In addition, total aMT6 was also determined for the aforementioned epoch.

RESULTS: Total sleep time was significantly greater with cherry juice than baseline and placebo trials (P = 0.003; 95% CI = 14.7 - 63.6 min). Sleep efficiency also showed improvements with the cherry juice over placebo trials (P = 0.017; 95% CI = 0.5 - 9.4%). Although circadian rhythm measures were not different, total aMT6 was greater with cherry juice (P < 0.001; 95% CI = 2519 - 5450 ng).

CONCLUSIONS: Tart cherry juice provides an increase in exogenous melatonin, improves sleep indices and hence the propensity for improved sleep quality in healthy adults. These findings present athletic and clinical populations with an alternative and viable solution for managing disturbed sleep.

1391 Board #172 MAY 30 11:00 AM - 12:30 PM

Dose Response Effects of Pomegranate Juice Concentrate Supplementation on DOMS

Daniel R. Machin, Kevin M. Christmas, Ting-Heng Chou, Sarah C. Hill, Douglas Van Pelt, Justin R. Trombold, Edward F. Coyle, FACSM. The University of Texas at Austin, Austin, TX.

(No relationships reported)

An acute bout of unaccustomed eccentric exercise causes prolonged strength loss and delayed onset muscle soreness (DOMS) for several days. Chronic dietary supplementation with polyphenols, from pomegranates, has been shown to accelerate recovery following eccentric exercise (Trombold et al. MSSE 42 (3) 493-498, 2010), however the optimal dose is unknown.

PURPOSE: To determine the effect of dietary supplementation with different doses of pomegranate juice concentrate (PJC) on isometric strength and soreness throughout a 96-hour period following an acute bout of eccentric exercise.

METHODS: Forty-five healthy, recreationally active males (22.3 +/- 4.0 y, 73.8 +/- 11.5 kg, 174.9 +/- 6.2 cm) were assigned to one of three treatment groups: Once-daily PJC (1×), twice-daily PJC (2×), or placebo (PLA) supplementation over a period of eight days. A 1× dose of PJC provided approximately 650 mg GAE. On day four of each treatment, subjects performed downhill running intervals (-10% decline) over a 40 minute period followed by 40 repetitions of eccentric elbow flexion at 100% of concentric 1-RM. Muscle soreness and maximal isometric strength of the elbow flexor and knee extensor muscles were assessed pre-exercise, 2, 24, 48, 72, and 96 hours post-exercise. Treatment comparisons were made using a one-way ANOVA with p < 0.05. Values were reported as mean +/- SD.

RESULTS: Throughout the 96-hour period after exercise, isometric elbow flexor strength was significantly higher in 1× and 2× groups as compared to PLA (main treatment effect, 83.6 +/- 2.7%, 85.6 +/- 1.9%, and 78.4 +/- 1.8%, respectively; p < 0.001). Isometric knee extensor strength was significantly higher in 1× and 2× groups as compared to PLA (main treatment effect, 93.9 +/- 1.5%, 91.6 +/- 1.5%, and 87.1 +/- 1.8%, respectively; p < 0.001). Muscle soreness peaked 24-48 hours following exercise, but was not different between treatments.

CONCLUSION: Dietary supplementation with 1× or 2× PJC results in higher isometric strength values compared to placebo for elbow flexor and knee extensor muscles during the 96-hour period after an acute bout of eccentric exercise. Once per day supplementation of PJC is equally effective as twice per day for recovery of isometric strength in the four days after downhill running or eccentric elbow flexion exercise.

Funded by POM Wonderful LLC

1392 Board #173 MAY 30 11:00 AM - 12:30 PM

Effects Of A Botanical Supplement On Recovery From Delayed Onset Muscle Soreness

Corey A. Rynders, Judy Y. Weltman, Chelsea Williams, Frank I. Katch, FACSM, Jay Hertel, FACSM, Arthur Weltman, FACSM. University of Virginia, Charlottesville, VA.

(No relationships reported)

PURPOSE: We examined the effects of a supplement containing a proprietary blend of botanical concentrates of Aphanizomenon flos-aquae (blue-green algae), antioxidants, enzymes, and D-Ribose (StemSport, Stemtech HealthSciences, Inc., San Clemente, CA) suggested to increase circulating stem cells, decrease local muscle inflammation, and attenuate exercise induced muscle damage on recovery from delayed onset muscle soreness (DOMS).

METHODS: Fifteen males (N=7) and females (N=8); age 24.4 ± 4.8 years; stature 171.9 ± 10.0 cm, mass 72.2 ± 14.7 kg) were randomized in a crossover, double-blind, placebo controlled trial to receive a placebo or supplement (4050 mg/day) for 14 days. DOMS was induced on day 7 for both placebo and active conditions in the non-dominant elbow flexor group with repeated eccentric contractions. Muscle swelling (biceps girth), elbow flexor isometric strength (hand held dynamometer), muscle pain/tenderness (visual analog scale), and range of motion (active elbow flexion and extension) were measured at baseline and at 24, 48, and 72 h post eccentric exercise. The crossover washout period was ≥14 days.

RESULTS: No significant condition-by-time interactions occurred between placebo and supplementation for the criterion measures of muscle swelling (p= 0.59), elbow flexion (p=0.27), isometric strength (p=0.74), pain (p=0.16), or tenderness (p=0.53). Decrements in elbow extension range of motion between 24 and 48 h post-exercise were less following supplementation (Δ elbow extension 24-48h post; supplement, -.04 ± 5.0 deg; placebo, -2.8 ± 5.5 deg; p=0.04).

CONCLUSIONS: Compared to placebo, supplementation did not significantly improve recovery outcome measures related to muscle recovery after upper-arm induced DOMS.

Supported in part by an unrestricted gift from Stemtech HealthSciences, Inc., San Clemente, CA. 92673. F.I. Katch, J. Hertel, and A. Weltman serve as scientific advisors to Stemtech HealthSciences.

1393 Board #174 MAY 30 11:00 AM - 12:30 PM

Can Therapeutic Use Of Terbutaline Be Distinguished From Doping Use With A Urine Sample

Jimmi Elers. Copenhagen University Hospital: Bispejberg, Copenhagen, Denmark.

(No relationships reported)

PURPOSE: We examined urine and serum concentrations after therapeutic use of single and repetitive doses of inhaled terbutaline and supratherapeutic use of a single oral dose of terbutaline.

METHODS: : We compared the concentrations in asthmatics with regular use of beta2-agonists prior to study and healthy subjects with no previous use of beta2-agonists. We enrolled 10 asthmatics and 10 controls in an open-label, cross-over study in which subjects were administrated 2 mg inhaled and 10 mg oral terbutaline on two study days. Further 10 healthy subjects were administrated 1 mg inhaled terbutaline every second hour (total 4 mg), which is the maximum permitted daily dose by the World Anti-Doping Agency (WADA). Blood samples were collected at baseline, 30 min, 1, 2, 3, 4, and 6 h after the first inhalations. Urine samples were collected at baseline, 0-4 h, 4-8 h, and 8-12 h after the first inhalations.

RESULTS: Median (IQR) urine concentrations peaked in the period 0-4 h after inhalation with Cmax 472 (324) ng x mL-1 in asthmatics and 661 (517) ng x mL-1 in healthy subjects, and 4-8 h after oral use with Cmax 666 (877) ng x mL-1 in asthmatic and 402 (663) ng x mL-1 in healthy subjects. All urine concentrations were corrected for the urine specific gravity.

CONCLUSION: In conclusion we found no significant differences in urine and serum concentrations between asthmatic and healthy subjects. We compared urine and serum concentrations after therapeutic inhaled doses and supratherapeutic oral doses and observed significant statistical diffences in both groups. We found high variability in urine concentrations between subjects in both groups. The variability between subjects was still present after the samples were corrected for urine specific gravity. When evaluating our results we found it impossible to distinguish between permitted therapeutic use and prohibited supratherapeutic use based on doping tests with urine and blood samples.

1394 Board #175 MAY 30 11:00 AM - 12:30 PM

Effect of Exercise and Aged Garlic Extract on Metabolic Parameters in High-Fat Diet-Induced Obese Rats

Dae Yun Seo1, Sung Ryul Lee1, Yeong Ho Baek2, Yi Sub Kwak3, Arturo Figueroa4, Nari Kim1, Byoung Doo Lee1, Kyuong Soo Ko1, Tae Hee Ko1, Jin Han1. 1Inje University, Busan, Korea, Republic of. 2Pusan National University, Busan, Korea, Republic of. 3Dong Eui University, Busan, Korea, Republic of. 4Florida State University, Tallahasse, FL.

(No relationships reported)

The worldwide prevalence of obesity is increasing at an alarming rate, with major adverse consequences for human health. Aged garlic extract (AGE) is widely promoted as a cholesterol-lowering agent and exercise alone is widely perceived to be beneficial for improving metabolic function in high fat diet (HFD) induced obese rats through reducing metabolic parameters.

PURPOSE: The purpose of this study was to investigate the effect of exercise and/or AGE on body weight, lipid profiles, inflammatory and oxidative stress markers in HFD induced obese rats.

METHODS: Forty Sprague Dawely rats were switched to HFD diet for 6 week and randomized into five groups: HFD (n=10), HFD with exercise (n=10), HFD with AGE (n=10), and HFD with exercise and AGE (n=10) for 4 weeks. AGE was administered orally at a dose of 2.86 g/kg. Exercise consisted of 15∼60 min of running 5 times/day with gradually increasing intensity.

RESULTS: AGE (p<0.01), Exercise, and Exercise with AGE (p<0.001) significantly decreased body weight gain, average weight gain and food efficiency rate compared to HFD. AGE significantly decreased visceral fat (p<0.05), and liver (p<0.01). Exercise significantly decreased visceral fat, and liver (p<0.01). In reducing visceral fat (p<0.001), epididymal fat (p<0.01) and liver weight (p<0.001), Exercise with AGE was effective but exercise showed stronger suppressing effect than AGE admin. Consumption of AGE significantly inhibited the increase in total cholesterol (65.1±10.0 vs HFD: 92.64±2.9) and low density lipoprotein-cholesterol (37.4±8.1 vs HFD: 52.1±18.6). C-reactive protein (CRP) and malondialdehyde (MDA) were significantly decreased compared to HFD (p<0.05).

CONCLUSION: AGE supplementation and exercise alone have anti-obesity effects AGE with exercise result in improvements of inflammatory and oxidative stress markers independently of changes in adiposity and lipids.

1395 Board #176 MAY 30 11:00 AM - 12:30 PM

Effects of Oxygenated Water on Aerobic Performance in Division II Collegiate Male Soccer Players

Tina M. Manos, Peter J. Fuller, Thomas J. Koesterer. Humboldt State University, Arcata, CA.

(No relationships reported)

Supplemental oxygen (O2), ingested in commercially-available “oxygenated water” preparations, has not been shown to have consistent effects on aerobic performance measures. Most researchers have found no significant differences in oxygen saturation of hemoglobin, VO2 max, and time to exhaustion when comparing the effects of oxygenated water to placebo; yet, in some studies, highly-fit subjects ingesting oxygenated water were found to have significantly higher values for these measures.

PURPOSE: To determine the effects of oxygenated water on aerobic performance in Division II male soccer players.

METHODS: A randomized, double-blind, cross-over design was used to study 12 Division II soccer players (age = 20.1 ±+ 1.4 years; mass = 73.3 ± 10.6 kg; height = 176.0 ± 6.9 cm) with the fastest two-mile run times (12:34 ± 0:19 min:sec) of their team (n = 20). Two maximal tests were conducted (8 mph, 2.5% grade initially, with increments of 2.5% grade every two minutes until volitional exhaustion) approximately 1 week apart; conditions were 500 ml of bottled water (placebo) or commercial “activated stabilized oxygen” water taken 15 min prior to the test. Time to exhaustion, oxygen saturation of hemoglobin (SpO2; via pulse oximetry), heart rate (HR), and expired gases were measured.

RESULTS: The SpO2 (95.58 ± 3.05 vs. 96.08 ± 2.46%; p = .497), HR (187.00 ± 8.66 vs. 187.16 ± 7.73 bpm; p = .942), and VO2 (57.26 ± 4.93 vs. 58.15 ± 3.87 ml/kg/min; p = .351) at the highest common workload achieved on both trials were not different between the placebo and oxygenated water conditions, respectively. The SpO2 at the lowest point during the maximal testing (94.58 ± 2.90 vs. 94.92 ± 2.06%; p = .732) and the SpO2 at exhaustion (94.75 ± 3.01 vs. 95.16 ± 2.24%; p = .610) were not different between conditions. VO2 max (59.97 ± 4.24 vs. 60.92 ± 3.51 ml/kg/min; p =.247) and maximum HR (192.08 ± 7.69 vs. 191.08 ± 7.63 bpm; p = .597) also were not significantly different between the conditions. Although the time to exhaustion (428.83 ± 62.54 vs. 451.17 ± 57.05 sec; p = .072) was longer by 22.34 seconds for oxygenated water versus placebo, the difference did not reach statistical significance.

CONCLUSION: Ingestion of oxygenated water did not result in any changes in aerobic performance measurements in Division II male soccer players.

1396 Board #177 MAY 30 11:00 AM - 12:30 PM

Polyphenol Rich Juice Supplementation in Olympic Swimmers does not alter Inflammation or Immune Biomarkers

Amy M. Knab1, Nicholas D. Gillitt2, Lynn Ciadella-Kam1, David C. Nieman, FACSM1, R. Andrew Shanely1. 1Appalachian State University, Kannapolis, NC. 2Dole Nutrition Research Institute, Kannapolis, NC.

(No relationships reported)

PURPOSE: This study investigated the effects of consumption of a juice blend made from whole fruits and vegetables on innate immunity, and chronic and acute inflammation in elite swimmers training three hours per day, compared to non-athletic controls.

METHODS: Nine male swimmers and seven controls were recruited and compared before and after a 10-day study period. Swimmers were randomized, and completed 10 days supplementation with or without 16 fl oz of the juice ingested pre- and post-workout, with a three week wash out period between supplementation periods. Blood samples were taken pre-supplementation, post-10 days supplementation, and immediately post-exercise on the 10th day.

RESULTS: Age was not different between swimmers (24.6 ± 0.7 y) and controls (25.7 ± 1.3 y). Swimmers were significantly fitter (VO2max = 53.1 ± 1.4 ml·kg·min, body fat = 11.7 ± 0.8 %) compared to controls (VO2max = 39.9 ± 2.7 ml·kg·min, body fat = 19.6 ± 1.6 %). All pre-exercise measures of inflammation and immune function were not different between swimmers and controls. The patterns of change (chronic) in inflammatory cytokines (IFN-γ, IL-1β, IL-6, IL-10, IL-8, IL-12p70, and TNF-α) and innate immune function (granulocyte and monocyte phagocytosis/ oxidative burst activity) were not different between juice and non-juice conditions over the 10-day study period. A single training bout at the end of the 10-day supplementation period caused a small but significant increase in plasma IL-6 (0.3 ± 0.1 to 0.8 ± 0.1 pg·ml with juice, and 0.5 ± 0.1 to 0.8 ± 0.1 pg·ml with no juice) and IL-10 (but not other cytokines), with no differences between juice and non-juice conditions. The training bout caused a small but significant increase in phagocytosis and oxidative burst activity, with no differences between juice and non-juice conditions.

CONCLUSIONS: Contrary to expectations, high-level training in elite swimmers was not associated with chronic inflammation or dysfunctional innate immunity, and acute changes post-3-h aerobic/anaerobic training bouts were mild or indicative of immune stimulation. The mixed fruit-vegetable juice supplement (∼9500 ORAC units) is a nutritious addition to the training diet, but has no influence on inflammation and innate immune measures that could be considered already at favorable levels. (Funded by Dole Foods Inc.)

1397 Board #178 MAY 30 11:00 AM - 12:30 PM

Effect of 4 Weeks Resveratrol Supplementation on Body Composition in Healthy Adults

Sarah J. Wherry1, James M. Smoliga2. 1Arizona State University, Phoenix, AZ. 2High Point University, High Point, NC.

(No relationships reported)

Resveratrol, a polyphenol found in grapes and other plants, has been demonstrated to have a number of beneficial health effects in laboratory models. Current evidence suggests there is much potential for resveratrol to decrease fat mass and increase muscle mass and bone mass. However, no human clinical trials have examined the effects of resveratrol supplementation on body composition.

PURPOSE: To evaluate the effects of 4 weeks oral supplementation of a resveratrol mixture (400mg 98% pure trans-resveratrol, 400mg grape extract, 100mg quercetin) on body composition parameters in healthy individuals, including total mass, fat mass, lean mass, and bone mass.

METHODS: 41 healthy adults (13 male, 28 female) between the ages of 18-75 were randomized to either the resveratrol (RES, n = 21) or placebo (PLA, n = 20) groups. Individuals participated in two testing visits: baseline (Pre) and post-supplementation (Post). Each visit included a fasting Dual-Energy X-ray Absorptiometry (DEXA) scan during which total body mass, fat mass, lean mass, and bone mass were measured. Following Pre, participants self-administered their respective supplement once daily for 4 weeks. Participants then underwent their Post DEXA scan. Data were analyzed using Repeated Measures ANOVA to determine if a significant (p ≤ 0.05) Group x Visit effect existed.

RESULTS: Total body mass was unchanged between groups (mean ± sd; PLA: Pre = 70.4 ± 14.1kg, Post = 70.2 ± 13.9kg; RES: Pre = 74.7 ± 14.7kg, Post = 74.8 ± 14.8kg; p = 0.317, power = 0.167). There were no significant Group x Visit effects for fat mass (PLA: Pre = 24.2 ± 9.5kg, Post = 24.3 ± 9.6kg; RES: Pre = 23.7 ± 9.3kg, Post = 23.7 ± 9.3kg; p = 0.664, power = 0.071), lean mass (PLA: Pre = 46.4 ± 10.3kg, Post = 46.1 ± 9.9kg; RES: Pre = 48.1 ± 10.2kg, Post = 48.3 ± 10.0kg, p = 0.195, power = 0.251), or bone mass (PLA: Pre = 2.83 ± 0.62kg, Post = 2.84 ± 0.63kg; RES: Pre = 2.87 ± 0.62kg, Post = 2.87 ± 0.63kg; p = 0.435, power = 0.120). DISCUSSION: It appears that short-term resveratrol supplementation does not alter body composition parameters in healthy adults. Further research using larger sample sizes, longer supplementation periods, and concurrent exercise in a variety of populations should be performed to better evaluate resveratrol’s efficacy.

1398 Board #179 MAY 30 11:00 AM - 12:30 PM

The Bioavailability Of Resveratrol (3,4’,5 Trihydroxystilbene)

Martin R. Lindley1, Annika Rasijeff1, Shannon R. Siegel2, Timothy D. Mickleborough, FACSM3. 1Loughborough University, Loughborough, United Kingdom. 2California State University , San Bernadino, San Bernadino, CA. 3Indiana University, Bloomington, IN. (Sponsor: T.D. Mickleborough, FACSM)

(No relationships reported)

PURPOSE: Resveratrol is one of many phenolic compounds that have been suggested as having potential applications in chronic disease prevention, however, most of the current evidence is based on in vitro findings. Given the potential benefits of resveratrol for combating chronic inflammatory diseases, it is important to evaluate the bioavailability of resveratrol especially as it has been suggested that the compound is rapidly metabolized and eliminated in humans.

METHODS: Twelve healthy, young males completed the study. Blood samples were collected at 30, 60 and 90 min following ingestion of a 1 g oral resveratrol dose (Transmax, Biotivia Bioceuticals,Vienna, Austria). Plasma samples were analyzed using high performance liquid chromatography and eluted resveratrol and metabolite peaks identified by mass spectrometry. The identity of resveratrol was confirmed by MS in plasma samples containing a standard at 100 ng/mL and 200 ng/mL concentrations with retention time identified by spikingOut of six peaks appearing on the HPLC chromatogram, the identities of resveratrol, and two metabolites were confirmed by MS (Metabolite B - mono-glucuronide; metabolite C- mono-sulfate conjugate).

RESULTS: The concentration of resveratrol (ng/mL) and metabolites (resveratrol equivalents in ng/mL) showed an increasing trend over time following ingestion. Plasma concentrations of resveratrol and metabolites at 30, 60 and 90 min post-dose were different from baseline (p< 0.008) except for metabolites C and E. There was an increase in the concentration of metabolite D: 181.3 ng/mL ± 284.4, 1325.9 ng/mL ± 1425.3, (p= 0.005) and metabolite F: 155.0 ng/mL ± 311.4, 1926.5 ng/mL ± 2365.9 (p= 0.005) between 30 and 60 minutes. Significant increases from 30 to 90 min were seen for metabolite B (438.4 ng/mL ± 756.3 to 1634.1ng/mL ± 1327.4, p= 0.006), metabolite D (181.3 ng/mL ± 284.4 to 1748.1 ng/mL ± 1578.0, p= 0.005 ), metabolite F (155.0 ng/mL ± 311.4 to 2783.7 ng/mL ± 2174. 3,p< 0.001) and resveratrol (p< 0.001). No differences were seen in resveratrol or any of its metabolites from 60 to 90 minutes (p>0.008).

CONCLUSIONS: Resveratrol has been shown to have low bioavailability as well as being lower than its metabolites and as such future research should focus not only on resveratrol bioavailability but also that of its metabolites.

1399 Board #180 MAY 30 11:00 AM - 12:30 PM

Effects of High Antioxidant Foods on Antioxidant Capacity in Post-Menopausal Women

Shelby Kloiber1, Robert Sawyer2, Melanie Hart1, Jacalyn McComb1. 1Texas Tech University, Lubbock, TX. 2Utah Valley University, Orem, UT.

(No relationships reported)

Oxidative stress brought on by free radicals can lead to an increased risk of certain diseases such as heart disease and some cancers. Oxidative stress mediated damage can be reduced by scavengers, or antioxidants that can eliminate the high reactivity of free radicals by turning them into non-radical and nontoxic metabolites. Many scientists have investigated the effects of different kinds of foods (whole, liquid, or supplement) to measure the change in oxidative damage and antioxidant capacity.

PURPOSE: To examine the effects of two types of foods high in antioxidants on antioxidant capacity in postmenopausal women.

METHODS: Healthy post-menopausal women without hormone replacement, (N=16) were randomly divided into four groups: fruits (F), soymilk (S), fruits and soymilk (S+F) and control (C). The intervention included a one week wash-out period where participants refrained from a list of foods high in antioxidants. The next four weeks involved a diet intervention. The S group was instructed to consume a total of 706 ml of approved soymilk throughout the day. The F group was instructed to consume 5 or more servings of fruit per day. The S+F group followed both the soymilk and fruit instructions. Subjects in the C group were instructed to maintain their normal diet. Fasting blood samples were drawn before and after the intervention. Spectrophotometric assays were conducted for antioxidant capacity by SOD (Cayman Chemical).

RESULTS: SOD showed a significant main effect for Test with the mean for the pre-test (M = 0.28 units/ml, SD = 0.15) being significantly lower than the mean for the post-test (M = 0.39 units/ml, SD = 0.23). There were no significant between group differences in SOD.

CONCLUSION: The results of a diet high in antioxidant rich food, specifically fruits and/or soymilk, after a 4 week time period, did significantly increase antioxidant levels. Both diets appear effective in increasing antioxidant capacity, with no diet being more effective than the other.

1400 Board #181 MAY 30 11:00 AM - 12:30 PM

Impact Of Intermittent And Continuous Physical Exercise On Endothelial Function And Oxidative Stress Postprandially

Robinson Ramirez-Velez1, Jose G. Ortega1, Ana C. de Plata Aguilar1, Cristina Araujo, Nut1, Celia Escobar Hurtado1, Ricardo A. Agredo Zuñiga1, Jorge H. Ramirez2. 1University of Valle, Cali, Colombia. 2University ICESI, Cali, Colombia.

(No relationships reported)

PURPOSE: To evaluate the impact of intermittent and continuous physical exercise on endothelial function and blood oxidative stress postprandially after a high-fat meal.

METHODS: Crossover trial in 14 healthy male subjects, 20 years of age, randomized to three experimental groups: (i) no exercise, (ii) 1 hour of moderate intensity continuous physical exercise, and (iii) 1 hour of moderate intensity intermittent physical exercise. Endothelium dependent flow mediated dilatation (FMD%) and plasmatic levels of nitrites and nitrates (NO2/NO3) were used to assess vascular endothelial function. Oxidative stress was evaluated using the total antioxidant capacity (TAC) test. Endothelial function and oxidative stress were assessed after 14 to 18 hours of physical inactivity (baseline); posterior measurements were performed 1h and 2h after the consumption of a high-fat meal.

RESULTS: No statistically significant differences were found on baseline FMD% in the three experimental groups. However, NO2/NO3 levels were significantly higher in the intermittent and continuous exercise groups compared to the control group (P<0.01). After the high-fat meal ingestion the FMD% decreased in the no-exercise and continuous exercise group but significatively increased in the intermittent exercise group. Furthermore, intermittent exercise was associated with increased TAC compared to no exercise and continuous exercise.

CONCLUSIONS: Moderate intensity intermittent physical exercise was found to be associated with beneficial effects on endothelial function and oxidative stress in the postprandial state. Randomized clinical trials assessing the effect of intermittent versus continuous physical exercise on hard outcomes of cardiovascular disease and metabolic syndrome are warranted.

1401 Board #182 MAY 30 11:00 AM - 12:30 PM

Antioxidant Supplementation To Prevent The Progression Of Microangiopathy In Hindlimb Unloading-induced Atrophied Soleus Muscle In Rats

Hidemi Fujino1, Miho Kanazashi1, Hiroyo Kondo2, Shinichiro Murakami1, Naoto Fujita1, Fumiko Nagatomo3, Akihiko Ishihara3. 1Kobe University Graduate School of Health Sciences, Kobe, Japan. 2Nagoya Women’s University, Nagoya, Japan. 3Kyoto University, Kyoto, Japan.

(No relationships reported)

PURPOSE: Exposure of skeletal muscle to oxidative stress increases the expression of important components of the proteasome proteolytic system and induces the damage of endothelial cell. The aim of this study was to explore the effects of the antioxidant supplementation during the phase of unloading on capillary regression in atrophied soleus muscle.

METHODS: Four groups of Wistar rats were studied: control (Con), control with ASX (Con+ASX), hindlimb unloading (HU), hindlimb unloading with astaxanthin (HU+ASX) groups. In the Con+ASX and HU+ASX groups, astaxanthin (ASX) was orally administered twice in a day for seven consecutive days (50mg/kg). Hindlimb unloading was applied to animals by suspending their tails for seven days. Intracellular ROS generation was determined by use of the redox-sensitive probe dihydroethidium. The capillary network of the soleus muscle was visualized using confocal laser microscopy. The expression levels of SOD-1 and VEGF protein were measured by immunoblotting and the mRNA expression levels of angiogenic factors (VEGF, KDR, Flt-1, Ang-1, Ang-2, Tie-2, and HIF-1 alpha) in the soleus muscle were determined by TaqMan probe-based real-time PCR.

RESULTS: HU resulted in an increase of ROS and a decrease of the number of capillaries, capillary volume, and capillary luminal diameter. Namely, the capillary network of the soleus muscle regressed in HU. In addition, HU caused the overexpression of SOD-1 and decreased the expression level of VEGF. However, the capillary network of the soleus muscle prevented disuse-induced capillary regression in HU+ASX. Furthermore, ASX supplementation abolished the unloading-induced overexpression in SOD-1 and attenuated the decrease in VEGF expression, and other angiogenic factors.

CONCLUSIONS: These results revealed that astaxanthin is effective to attenuate the development of oxidative stress and maintain the architecture of capillary network in disused skeletal muscle.<br

Supported by Grants-in-Aid for Science Research from the Japanese Ministry.

1402 Board #183 MAY 30 11:00 AM - 12:30 PM

Uric Acid Is A Key Antioxidant Against Iron-mediated Oxidative Stress In Resistance/power Exercise.

Marcelo P. Barros1, Leandro Lorenço-Lima1, Douglas Ganini2, Cristina V. Vardaris1, Etelvino J.H. Bechara3, Rui Curi4, Tacito P. Souza-Junior5. 1Universidade Cruzeiro do Sul, Sao Paulo, SP, Brazil. 2National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC 27709, NC. 3Universidade Federal de Sao Paulo (UNIFESP), Diadema, SP, Brazil. 4Universidade de Sao Paulo, Sao Paulo, SP, Brazil. 5Universidade Federal do Parana, Curitiba, PR, Brazil. (Sponsor: Tania Pithon-Curi, FACSM)

(No relationships reported)

Reactive oxygen species (ROS) are normally produced in humans during endurance (mainly by mitochondrial electron transport system) or resistance exercise (augmented xanthine oxidase activity in cytosol). Drastic drops in performance, precocious fatigue, exacerbated inflammation processes, and injury risk are regular outcomes of the excessive production of ROS in athletes. Paradoxically, balanced ROS production is also essential to provide the desired physiological adaptations of training and also the health benefits from exercise. Furthermore, iron homeostasis is recognized as a key factor in controlling oxidative stress during exercise.

PURPOSE: This work aims to evaluate key biomarkers of iron-related oxidative stress in plasma of young athletes after an anaerobic power test.

METHODS: Young male college athletes (n = 17; age, 23.1 ± 5.8 years; height, 175.4 ± 2.3 cm; weight, 81.1 ± 9.3 kg; from Universidade Metodista de Santos, Brazil) were selected. A Wingate Test was carried out using a Cybex cycle ergometer with increasing loads up to 10% BW (after familiarization and pre-heat procedure for 5 min). Blood samples (5 mL) were collected before (t0) and 5/60 min after (t5 and t60) the Wingate test, and total iron, heme-iron, uric acid, xanthine oxidase activity (XO), ferric-reducing activity (FRAP), and malondialdehyde (MDA, biomarker of lipid oxidation) were measured in plasma. RESULTS:Plasma iron content was increased immediately after Wingate (up to 3-fold, at t5), whereas heme-iron content only varied slightly (25% maximum). However, total FRAP levels varied consistently with total iron (R2 = 0.939) in order to limit iron-mediated lipid oxidation in plasma. Remarkably, uric acid - a major purine catabolite from energy(ATP)-depleted muscles - accumulated in plasma lately (25% higher at t60) to adequately provide an extended antioxidant capacity, as shown by its high correlation with FRAP (R2 = 0.788).

CONCLUSIONS: Even under exhaustive conditions, there is a tight control of iron homeostasis in plasma of athletes, and uric acid, massively produced by exhausting muscles during resistance/power exercise, contributes to restrain oxidative stress in plasma based mostly on its iron-chelating capacity, identified here by FRAP activity. Financial support: FAPESP, CAPES & CNPq (Brazil).

1403 Board #184 MAY 30 11:00 AM - 12:30 PM

The Effect of Quercetin Supplementation on Volitional Fatigue and Skeletal Muscle Mass Retention During Cancer Cachexia

Justin P. Hardee, Kandy T. Velazquez, Melissa J. Puppa, Johannes D. Aartun, Aditi A. Narsale, Shuichi Sato, J Mark Davis, FACSM, James A. Carson, FACSM. University of South Carolina, Columbia, SC.

(No relationships reported)

Cancer cachexia (CC) has been defined as an unintentional 10% loss of body weight (BW) over a 12-month period that occurs in the presence of an underlying disease. Two common hallmarks of CC are decreased muscle mass and hypogonadism. The APCMin/+ mouse is an IL-6 dependent model of CC.

PURPOSE: To determine the effects of quercetin supplementation on volitional grip strength, muscle mass, and testes size during the progression of CC.

METHODS: At 15 weeks of age, male C57BL/6 and APCMin/+ mice were randomly assigned to vehicle (B6 and Min-V) or quercetin supplementation (Min-Q) for 3 weeks. Quercetin was administered via oral gavage daily at a dose of 25 mg/kg of BW. Grip strength was measured pre- and post-supplementation.

RESULTS: Cachexia decreased grip strength 19% (B6: 2.42 ± 0.07 N vs Min: 2.01 ± 0.08 N; p < 0.001); however, quercetin maintained grip strength over 3 weeks (Min-V: 1.67 ± 0.08 N vs Min-Q: 2.09 ± 0.13 N). Bodyweight decreased 13% (p < 0.001) with cachexia; while quercetin attenuated BW loss (Min-Q: -7%). Cachexia decreased gastrocnemius (GAS) muscle mass 33% and rectus femoris (RF) muscle mass 37% (p < 0.05); whereas quercetin attenuated muscle mass loss. Cachexia reduced testes size 29% (p < 0.01) and quercetin attenuated the loss (Min-Q: 12%). Min GAS and RF muscle mass were correlated with testes size (R2 = 0.68 and 0.62, p < 0.05, respectively).

CONCLUSION: Quercetin supplementation for 3 weeks maintained volitional grip strength in mice undergoing cachexia, which may be due to the retention of muscle mass and testes size. Funded by NCI R01-CA121249

A-37 Free Communication/Poster - Occupational Physiology

MAY 30, 2012 7:30 AM - 12:30 PM

ROOM: Exhibit Hall

1404 Board #185 MAY 30 11:00 AM - 12:30 PM

Daily Aspirin Therapy Does Not Increase Body Core Temperature Responses in Firefighters During Exertion in Thermal Protective Clothing

Serina J. McEntire, Steven E. Reis, Diane Comer, Charity G. Moore, Riana R. Pryor, Jennifer Erin, Priya Khorana, Joe Suyama, Francis X. Guyette, David Hostler. University of Pittsburgh, Pittsburgh, PA. (Sponsor: Robert Robertson, PhD, FACSM)

(No relationships reported)

Heart attack is the most common cause of line-of-duty deaths in the fire service. Daily aspirin therapy is a common preventative measure used to reduce the morbidity of heart attacks in the general population. However, the mechanism of aspirin can hypothetically impair thermoregulation and anecdotal reports have suggested aspirin use increases the risk of heat illness. In spite of these observations, the safety profile of aspirin during uncompensable heat stress has not been well described.

PURPOSE: Determine if fourteen days of daily aspirin therapy (81mg PO) alters body core temperature responses during exertion in the heat while wearing thermal protective clothing (TPC).

METHODS: In this double-blind, placebo controlled study, 102 firefighters were randomized to receive either 14 days of aspirin (N=54) or a placebo (N=48) prior to completing a 50 minute bout of treadmill exercise in the heat (38.9±1.1°C; 24±7% RH) while wearing TPC and self contained breathing apparatus. Heart rate, weighted mean skin temperature, and body core temperature were monitored throughout the 50 minutes of exercise.

RESULTS: There were no differences in age, height, mass, BMI, or VO2max between groups. Heart rate at the end of exercise did not differ between aspirin and placebo groups (174±17 vs. 171±17 bpm, respectively). In the aspirin group, baseline core body temperature was 37.1±0.3°C, which was similar to the placebo group (37.2±0.5°C; p=0.55). Maximal body core temperature was 38.7±0.5°C in the aspirin group and 38.6±0.5°C in the placebo group (p=0.39). The percent change in core temperature from baseline was 4.3±1.2% in the aspirin group and 3.9±1.6% in the placebo group (p=0.20). Upon completion of exercise, maximal weighted skin temperature was 38.3±0.5°C in the aspirin group and 38.1±0.6° in the placebo groups. Skin temperature rose 12.5±2.3% from baseline in the aspirin group and 12.2±2.6% with the placebo (p=0.55).

CONCLUSIONS: Fourteen days of aspirin therapy does not alter temperature responses among firefighters performing exertion in the heat.

1405 Board #186 MAY 30 11:00 AM - 12:30 PM

Physiological and Thermoregulatory Responses to Wearing N95 Filtering Facepiece Respirators

Jung-Hyun Kim, Raymond J. Roberge, Stacey M. Benson. CDC/NIOSH/NPPTL, Pittsburgh, PA.

(No relationships reported)

The use of filtering facepiece respirators (FFR) has increased enormously in both public and occupational sectors for respiratory protection from infectious airborne particles. The N95 class of FFR, which filters out at least 95% of airborne particles >0.3 μm, is the most common type of FFR; however, there has been a lack of scientific information on physiological and thermal burdens of wearing N95 FFR.

PURPOSE: To investigate physiological and thermoregulatory responses to wearing N95 FFRs while performing low-moderate intensity exercise.

METHODS: Twenty healthy subjects (13 men, 7 women), who passed a physical examination and a respirator quantitative fit test, performed three trials of treadmill exercise (5.6 km/h, 0% grade, 60min duration) in a thermoneutral environment (21.4±0.7°C, 23.5±7.9%) while wearing two popular styles (cup-shaped and flat fold) of N95 FFR or not wearing FFR for control (CON). Study variables included core body temperature (Tco), skin temperature at the cheek (Tcheek) and abdomen (Tabdomen), heart rate (HR), respiratory rate (RR), transcutaneous carbon dioxide (tcPCO2), and oxygen saturation (SpO2). Deadspace microclimate temperature and humidity were also measured in FFR trials. Data were analyzed by two-way (Trials × Time) repeated measures ANOVA.

RESULTS: Tco, Tcheek, and Tabdomen rose significantly by time in all trials (p<0.001) and the final deadspace microclimate temperature and humidity reached up to 33°C and 90%, respectively. However, there was no statistical difference in these temperature variables between CON and the FFR trials. HR and RR increased significantly by time in all trials (p<0.001), but at a greater rate in the FFR trials than in CON (HR; P<0.001, RR; P<0.01). There was no statistical difference in SpO2 levels between CON and FFR trials, however, tcPCO2 levels were significantly higher in the FFR trials than in CON (p<0.001).

CONCLUSION: Wearing N95 FFR while performing low-moderate work for 60 min in a thermoneutral environment did not impose a significant thermal burden on the wearer. However, significantly elevated HR and RR in the FFR trials indicated that wearing N95 FFR may add additional metabolic demands. Also, breathing through N95 FFR did not interfere with SpO2, but caused some mild degree of CO2 retention (tcPCO2 ≥ 45 mmHg).

1406 Board #187 MAY 30 11:00 AM - 12:30 PM

Increased Salivary Cortisol and State Anxiety Levels in Firefighter Candidates during Repeated Fire Suppression Tasks

Deena Campbell1, Heather E. Webb2, Christopher J. Jackson1, Daniel Cross3. 1University of New South Wales, Sydney, Australia. 2Mississippi State University, Starkville, MS. 3Mississippi State Fire Academy, Jackson, MS.

(No relationships reported)

Research suggests that the combined psychological and physiological stress load of firefighting may contribute to an exacerbated cortisol response during fire suppression.

PURPOSE: The purpose of this study was to examine the salivary cortisol (SCORT) response in firefighter candidates during simulated fire suppression tasks.

METHODS: Twelve firefighter candidates (age = 25.25 ± 5.69) participated in two research sessions, an informational session during which consent and demographic/psychometric data were obtained, and a second session in which the candidates completed two fire suppression evolutions. In each evolution, the participants entered the burn building in pairs and undertook fire suppression tasks. Baseline measures of SCORT and state anxiety (SAI) were collected immediately prior to each evolution, and collected again within 5 minutes of completion of each evolution.

RESULTS: The fire suppression tasks resulted in elevated SCORT levels (when controlling for participant order) compared to baseline in both evolution 1 and 2 (t = 11.27, p = 0.004 and t = 44.07, p = 0.000 respectively). Anxiety levels also increased but did not reach significance in evolution 1, and increased significantly in evolution 2 (t = -2.66, p = 0.026).

CONCLUSIONS: These finding suggest that the candidates experienced both physical and psychological stress in both evolutions. Further, findings suggest that the experience of the first evolution did not reduce the psychological stress and the resultant physiological load of the second evolution.

1407 Board #188 MAY 30 11:00 AM - 12:30 PM

Review of Current Minimum Riding Weights in Australian Horse Racing - Implications for Jockey Health and Wellbeing

Caron B. Jander1, David A. Greene2, Helen O’Connor3, Giles D. Warrington, FACSM4. 1Australian Racing Board, Freshwater, Australia. 2Australian Catholic University, Strathfield, NSW, Australia. 3University of Sydney, Lidcombe, NSW, Australia. 4Dublin City University, Dublin, Ireland.

(No relationships reported)

Despite the international popularity of horse racing there is a dearth of population specific research available describing the physiological demands and health and lifestyle characteristics of jockeys. Given the weight-restricted nature of horse racing, jockeys often engage in extreme and unhealthy weight-loss practices, which are likely to place riders at an increased risk of injury. As a consequence, the Australian Racing Board (ARB) commissioned a scientific review of the minimum riding weight for flat jockeys.

PURPOSE: The purpose of this review was to identify potential health issues associated with jockeys complying with current stipulated weight standards operating in Australian horse racing.

METHODS: The review comprised (i) a comprehensive review of national height and weight statistics, (ii) a review of the international scientific literature on population specific normative data , (iii) evaluations of race-day jockey urine samples collected on 875 jockeys over the past 3 years in New South Wales for determination of urine specific gravity (Usg) (iv) and the comparison of the results of Usg samples collected for the purpose of drug testing in the work place in the state of Victoria on 452 petrochemical workers (control group) and 305 jockeys at the race course.

RESULTS: Jockeys were found to have 8 key health issues associated with race riding. Issues included: ‘Making weight’; poor dietary practices; compromised bone health; dehydration; smoking; engaging in a high risk sport; lifestyle challenges; and mood disorders. Disturbing levels of consistent and pervasive dehydration were found in jockeys both on race day and non-race days. Results indicate that on race day 48.4% of 875 samples indicated significant dehydration,(Usg ≤1.025), 27% had moderate to high levels of dehydration (Usg<1.025 and ≥ 1.02), 24.5 % within the dehydration threshold as defined by Sawka et al., 2007 (Usg ≤1.020)

CONCLUSION: Based on these findings, the following recommendations were presented to the ARB; (i) increase the minimum riding weight by 1 kg to 54 kg; and (ii) increase the median and maximum riding weight by 1 kg. It is anticipated that immediate health outcomes will result in a decrease in the extreme dehydrating practices currently employed by many jockeys.

1408 Board #189 MAY 30 11:00 AM - 12:30 PM

Anthropometric Measurements of Obesity and Their Link to Lifestyle and Cardiovascular Risk in Colorado Firefighters

Tiffany Lipsey, Lorin O’Toole, Tracy Nelson, Jennifer Peel, Richard G. Israel, FACSM. Colorado State University, Fort Collins, CO.

(No relationships reported)

Cardiovascular disease (CVD) is the leading cause of death in firefighters as it is in the general population. Despite data promoting Colorado as the leanest state in the nation and the image of firefighters as healthy and physically fit, obesity is evident in Colorado firefighters and continues to be an important CVD risk factor. Job performance and health outcomes among those in public safety are greatly impacted by obesity despite the perception of the general public that firefighters are healthy and fit.

PURPOSE: To determine obesity prevalence, depending on measurement and classification, and its association with lifestyle factors and cardiovascular (CV) risk in a cohort of Colorado firefighters.

METHODS: Analyses were conducted on data from 466 Colorado firefighters (41 females; 425 males; mean age 38 y). Using standard classification cut-points, rates of obesity were determined using body mass index (BMI), waist circumference (WC), sagittal abdominal diameter (SAD), and percent body fat (%BF) from skin fold (SF) and hydrodensiometry (H) measurements. Lifestyle factors used in the analysis included diet, physical activity, sleep, and stress. Lipids, C-reactive protein (CRP), predicted maximal oxygen consumption and strength measures were also included. CV risk was also assessed using the Cooper Risk Profile. Correlation statistics were run for each anthropometric measure with the above variables.

RESULTS: Obesity prevalence varied by measurement in females and males respectively: BMI=9.8 %, 19.1%; WC=19.5%, 19.8%; SAD=31.6%, 43.5%; %BF(SF)=17.1%, 15.1%; and %BF(H)=23.7%, 28.6%. In both sexes, anthropometric measures were positively correlated with triglycerides and CRP but inversely associated with high-density lipoprotein cholesterol, sit & reach, and estimated maximal oxygen consumption (VO2max)(besides BMI in females ) (p≤0.05). All anthropometric measures were significantly correlated with CV risk (p≤0.05) except WHR in females. The strongest link to CV risk was %BF(SF) in females and WHR in males.

CONCLUSIONS: The rate of obesity in Colorado firefighters varies depending on the measure used. There are significant associations between obesity, lifestyle factors and CV risk that should be further explored in light of the already increased risk for CVD in firefighters.

1409 Board #190 MAY 30 11:00 AM - 12:30 PM

The Beneficial Effects Of Walking Following Repeated Bouts Of Sustained Static Postures

François Taillefer, Jean P. Boucher, FACSM, M Zummo, R Savard, A S. Comtois. University of Quebec in Montreal, Montreal, QC, Canada.

(No relationships reported)

PURPOSE: We have shown that 20 min of various sit, sit-stand and stand static postures interspersed by 5 min walking periods was beneficial for lower limb muscle blood flow and oxygenation (Taillefer et al., 2011). However, the physiological impact of walking on the lower limbs following static postures remains to be shown, especially in individuals confronted with varicose veins. Thus, the present study measured the physiological effects of a 5’ walk following repeated bouts of 20’ static periods.

METHODS: Two groups (n = 10 per group) of women (without varicose veins, C0 and with varicose veins, C2) were studied during the maintenance of 6 twenty minute periods of different static postures all interspersed by 5 min walking periods. The following measurements were continuously recorded on the left lower limb during each static and walking periods: blood perfusion (foot), transcutaneous partial pressure (TcP) of O2 and CO2 (foot), cutaneous temperature (Ct, medial malleola), EMG of the gastrocnemius muscle, VO2, and heart rate (HR).

RESULTS: Physiological variables were pooled since no significant differences were observed between both groups (C0 vs C2). The variables were significantly (p<0.05) modified during walking when compared to the static postures (blood perfusion, 120.0 ± 35.31 vs 5,7 ± 1.79 perfusion units; TcPO2, 75.7 ± 19.56 vs 59.4 ± 16.77 mmHg; TcPCO2, 27.48 ± 5.83 vs 33.1 ± 5.82 mmHg; Ct, 28.4 ± 1.60 vs 27.8 ± 1.34; EMG, 23.4 ± 9.50 vs 2.3 ± 2.50 mV; VO2, 629.9 ± 195.50 vs 219.9 ± 40.10 ml*min-1; HR, 84.7 ± 10.40 vs 72.5 ± 10.60 b*min-1, respectively).

CONCLUSIONS: The varicose vein group (C2) responded very similarly to the non varicose vein group (C0) during walking. These results indicate the importance of walking to maintain lower limb oxygenation to possibly minimize the cascade of physiological events leading to the appearance of varicose veins, especially when these individuals have to maintain prolonged static postures.

1410 Board #191 MAY 30 11:00 AM - 12:30 PM

The Effect of Pre-cooling on Cardiovascular and Metabolic Strain During Incremental Exercise

Eric M. Hultquist1, Logan Arena1, Welsey K. Lefferts1, Thomas W. Storer2, Christopher B. Cooper, FACSM2, Patricia C. Fehling, FACSM1, Denise L. Smith, FACSM1. 1Skidmore College, Saratoga Springs, NY. 2UCLA, Los Angeles, CA.

(No relationships reported)

Pre-cooling has been shown to improve endurance performance and to lessen heart rate (HR) for a given level of work. However, the effect of pre-cooling on the relationship between HR and oxygen uptake (VO2) has not been well studied.

PURPOSE: To investigate the relationship between cardiovascular and metabolic responses during maximal exercise performed at either normal or depressed core temperature (Tco).

METHODS: Ten men (age, 21 ± 1 yr; VO2max, 60.2 ± 6.9 ml·kg-1·min-1) performed a maximal treadmill test following two conditions: (a) control (CON), in which participants rested in a thermoneutral laboratory (21.6 ± 4.8 °C), and (b) pre-cooling (PC), during which participants were submerged up to their armpits in 23.1 ± 0.2 °C water for 24 min. The chronotropic index (CI) was derived from the slope of the regression line between HR and VO2. A repeated measures ANOVA was employed to examine differences in Tco, HR, and VO2 and the CI. Significance was set at P < 0.05.

RESULTS: Prior to the onset of exercise Tco was significantly lower for PC (36.69 ± 0.32 °C) compared to CON (37.12 ± 0.39 °C; P < 0.05). Time to fatigue was significantly longer in the CON (914 ± 97 s) compared to the PC condition (889 ± 97 s). Tco during exercise remained consistently lower for PC compared to CON (P < 0.05). Furthermore, there was a significant condition x time interaction for Tco between conditions (P < 0.05). VO2 was not significantly different between conditions. PC resulted in an 8-10 b·min-1 lower HR than the CON condition at any given time point during the exercise protocol and at peak (PC, 178 ± 9 b·min-1; CON, 188 ± 6 b·min-1). The CI did not differ between conditions; however, the y-intercept was significantly lower for PC compared to CON (PC, 53.6 ± 11.0; CON, 67.3 ± 11.0).

CONCLUSION: Reductions in Tco significantly reduced cardiovascular strain (HR) at any given workload and decreased time to fatigue. However, pre-cooling did not change VO2 or alter the relationship between HR and VO2 (CI). Although there was no change in the CI, pre-cooling decreased the HR response, shifting the regression line down. These results suggest potential use of pre-cooling to mitigate cardiovascular strain in firefighters.

Supported by contract from DHS Science and Technology (UCLA contractor).

1411 Board #192 MAY 30 11:00 AM - 12:30 PM

Cardiovascular and Metabolic Responses During Maximal Incremental Exercise in Firefighter’s Personal Protective Equipment

Wesley K. Lefferts1, Eric M. Hultquist1, David A. Barr1, Thomas W. Storer2, Christopher B. Cooper, FACSM2, Patricia C. Fehling, FACSM1, Denise L. Smith, FACSM1. 1Skidmore College, Saratoga Springs, NY. 2UCLA, Los Angeles, CA.

(No relationships reported)

Personal protective equipment (PPE) is worn by firefighters to protect them from work related hazards. Work in PPE increases the metabolic cost of a given work load. Due to added weight, restrictive properties and the circulatory effects of full encapsulation, working in PPE may affect the relationship between heart rate (HR) and oxygen uptake (VO2) as expressed as the chronotropic index (CI) (slope of the regression line for HR vs.VO2); however, the extent to which the physiological stress imposed by PPE impacts the CI is unknown.

PURPOSE: To investigate the effects of PPE on cardiovascular and metabolic responses during incremental exercise and to determine if PPE affects the CI.

METHODS: HR and VO2 were measured in 10 male participants (age, 21 ± 2 yr; height, 178 ± 8 cm; weight, 76.2 ± 3.1 kg; VO2max, 51.9 ± 5.0 ml·kg-1·min-1) when performing graded exercise tests across 3 conditions: control (CON; wearing athletic attire), wearing PPE (PPE) and weighted vest (WV) (weight equal to PPE).

RESULTS: Time to exhaustion was significantly different across all 3 conditions (CON, 866 ± 121 s; PPE, 582 ± 92 s; WV, 671 ± 105 s; (P < 0.01). Over time, submaximal VO2 and HR were significantly lower in the CON condition (P < 0.01) compared to the PPE and WV conditions, which were similar to each other. Peak HR was significantly higher in the CON (191 ± 11 b·min-1) condition compared to the WV (187 ± 10 b·min-1) and PPE (183 ± 9 b·min-1) conditions (P < 0.01). There was a tendency for peak HR in the PPE condition to be lower than the WV condition (P = 0.07). VO2max was significantly different among all 3 conditions (P < 0.01). VO2max was 9.4% higher in the CON (3.96 ± 0.5 l·min-1) condition compared to the PPE (3.62 ± 0.4 l·min-1) condition (P < 0.01). VO2max was 4.8% higher in the CON condition compared to the WV (3.78 ± 0.4 l·min-1) condition; however, this was not statistically significant (P = 0.07). The CI (CON, 31.8 ± 4.4; PPE, 31.7 ± 5.4; WV, 32.6 ± 4.6) and y-intercept (CON, 72.1 ± 20.7; PPE, 74.6 ± 23.8; WV, 72.0 ± 18.8) were similar across all conditions.

CONCLUSION: The present study has shown that increasing the physiological strain with PPE or equivalent weight increases HR and VO2; however, the tight linear relationship between HR and VO2 (CI) remains unaffected.

Supported by contract from DHS Science and Technology (UCLA contractor).

1412 Board #193 MAY 30 11:00 AM - 12:30 PM

The Relationship Between Bmi And A Graded Maximal CPR Compression Test

Patrick W. Davidson1, Christopher C. Dunbar, FACSM2, Robert Curran3, Paul F. Martinowicz2. 1Springfield College, Springfield, MA. 2Brooklyn College, Brooklyn, NY. 3Brooklyn College, Brooklyn College, NY.

(No relationships reported)

PURPOSE: To determine if there was a relationship between subject BMI and VO2peak during an Incremental Work Test using a CPR simulator (IWT)

METHODS: Eleven male subjects (age: 23 +/- 4 yrs; body mass: 81.06 +/- 11.40 kg; BMI: 25.86 +/- 3.03 kg/m2) underwent the following conditions: 1) Incremental Work Test (IWT): using a CPR simulator where the cadence of CPR compressions increased every 30 sec to volitional fatigue 2) Treadmill VO2max (TM): incremental oxygen consumption (VO2) test using the Bruce Protocol. During all conditions VO2 and Heart Rate (HR) were measured using a Parvo-Medics True One 2400 Metabolic Measurement System (Sandy, Utah).

RESULTS: The VO2peak measured during maximal graded treadmill testing using the Bruce Protocol averaged 38.13 mL/kg/min +/- 5.98. The VO2peak measured during maximal graded CPR compression testing averaged 14.76 mL/kg/min +/- 3.46. There was no relationship found between subject BMI and TM; however, a significant (p < .05) inverse relationship was found between subject BMI and IWT

CONCLUSION: A significant inverse relationship was found between BMI and IWT. The results indicate that the larger an individual is relative to height, the poorer the performance during repeated CPR compressions irrespective of cardiorespiratory fitness as measured by treadmill exercise.

1413 Board #194 MAY 30 11:00 AM - 12:30 PM

Changes in Physical Fitness and Anthropometry of Police Academy Cadets During a 16-Week Physical Training Program

Ross A. Sherman, Amy A. Crawley, William R. Crawley, William J. Burgess, III. Grand Valley State University, Allendale, MI. (Sponsor: Jeffrey A. Potteiger, FACSM)

(No relationships reported)

Law-enforcement organizations require their employees to attain and maintain a threshold level of physical fitness so they are ‘fit to work’. As part of a college-run police academy, cadets undertake a structured 16-week physical training program.

PURPOSE: To describe changes in physical fitness and anthropometry of police academy cadets during their 16-wk physical training program.

METHODS: 15 cadets (24±4 yrs; 1.80±0.08 m), thirteen males and two females, were tested in Wks 1, 8, and 16 of their 3-day per wk police academy training program. A battery of tests was used to assess physical fitness (flexibility, vertical jump, grip strength, sprint speed, and agility) and anthropometrical characteristics (body mass and body fat) and . Time-related changes in each test are shown as mean difference and 95% confidence interval (CI).

RESULTS: Only 40-yd sprint time substantially improved during the 16-wk program; specifically between Wk 1 and Wk 8 (-0.45 [-0.78 to -0.12] s). However, flexibility was found to marginally decrease during the 16-wk program (Wk 1-16: -5.4 [-10.9 to 0.1] cm). The remaining markers of physical fitness did not change (see Table 1.).

Table 1

Table 1

Neither body mass (Wk 1-16: -1.9 [-15.0 to 11.1] kg) nor body fat (Wk 1-16: -0.6 [-4.6 to 3.3]%) changed during the course of the 16-wk training program.

CONCLUSION: The 16-wk physical training program currently used had little or no impact on pre-academy physical fitness or anthropometric characteristics. Only 40-yd sprint performance improving during the first eight week block, and there were no improvements in any test during the second eight week block.

1414 Board #195 MAY 30 11:00 AM - 12:30 PM

Wildland Firefighters Demonstrate Seasonal Acclimatization to the Heat Despite No Changes in Aerobic Fitness

Brianna Lui, Walter S. Hailes, John S. Cuddy, Brent C. Ruby, FACSM. Montana Center for Work Physiology and Exercise Metabolism, The University of Montana, Missoula, MT.

(No relationships reported)

Wildland fire suppression involves long hours of strenuous work across rugged terrain with frequent exposure to high ambient temperatures. These physical demands of the job, coupled with the environmental conditions, increase the probability of heat related injury (HRI). Although heat acclimatization may mitigate the risk of HRI, the degree to which wildland firefighter’s (WLFF) demonstrate seasonal acclimatization is unknown.

PURPOSE: To determine the physiological changes associated with heat acclimatization across the 4-month fire season in the western United States.

METHODS: (n=26 males) 12 WLFF (27 ± 7 yrs; 179 ± 7 cm; 79 ± 10 kg) and a comparison group of 14 non-WLFF (25 ± 4 yrs; 180 ± 7 cm; 77.7 ± 8.8 kg) completed a 60-min heat stress trial (treadmill walking at 50% VO2max) in a climate controlled chamber (43.3°C, 33% RH) prior to and following the western Montana fire season (May and September, respectively). VO2max and body composition was also measured pre- and post-season. Core temperature, heart rate, and physiological stress index (PSI) was continuously monitored and analyzed using repeated measures ANOVA. A significance level was set at p<0.05.

RESULTS: VO2max did not change for the WLFF across the season. In contrast, non-WLFF demonstrated a significant increased in VO2 max (55.9 ± 7.8 ml/kg/min vs. 58.7 ± 8.6 ml/kg/min pre and post season, respectively, p<0.05). Neither group demonstrated significant seasonal differences in the heart rate response to the heat stress trials. In contrast, WLFF demonstrated a significant seasonal reduction in core temperature during the heat stress trials at 45-min (38.1 ± 0.31°C vs. 38.2 ± 0.30°C, p=0.032) and 60-min (38.2 ± 0.39°C vs. 38.5 ± 0.34°C, p=0.022). WLFF PSI also demonstrated a significant seasonal decrease from 5.84 ± 1.3 to 5.13 ± 1.2 (p<0.05, main effect of season). There were no differences in core temperature or PSI for the non-WLFF.

CONCLUSIONS: WLFF demonstrate significant changes in seasonal acclimatization to exercising in the heat independent of changes in aerobic capacity. This suggests that long-term exposure to the environment strongly drives adaptation to hot environments.

1415 Board #196 MAY 30 11:00 AM - 12:30 PM

Deployment of an Advanced Electrocardiographic Analysis (A-ECG) to Detect Cardiovascular Risk in Career Firefighters

Brett A. Dolezal1, Tom Storer1, Marlon Abrazado1, Reed Watne1, Todd Schlegel2, Maxim Batalin1, William Kaiser1, Denise Smith, FACSM3, Christ Cooper, FACSM1. 1UCLA, Los Angeles, CA. 2NASA Johnson Space Center, Houston, TX. 3Skidmore College, Saratoga Springs, NY.

(No relationships reported)

INTRODUCTION: Sudden cardiac death is the leading cause of line of duty death among firefighters, accounting for approximately 45% of fatalities annually. Firefighters perform strenuous muscular work while wearing heavy, encapsulating personal protective equipment in high ambient temperatures, under chaotic and emotionally stressful conditions. These factors can precipitate sudden cardiac events like myocardial infarction, serious dysrhythmias, or cerebrovascular accidents in firefighters with underlying cardiovascular disease.

PURPOSE: The purpose of this study was to deploy and then evaluate the contribution of resting advanced ECG (A-ECG) in addition to other screening tools (family history, lipid profiles, and cardiopulmonary exercise tests, XT) in assessment of an individual’s cardiac risk profile.

METHODS: Forty-four career firefighters were recruited to perform comprehensive baseline assessments including tests of aerobic performance, fasting lipids and glucose. Five-min resting 12-lead A-ECGs were obtained in a subset of firefighters (n=21) and transmitted over a secure networked system to a NASA physician collaborator. Using myocardial perfusion and other imaging as the gold standard, A-ECG scoring has been proven useful in accurately identifying a number of cardiac pathologies including coronary artery disease (CAD), left ventricular hypertrophy, hypertrophic cardiomyopathy, and non-ischemic and ischemic cardiomyopathy.

RESULTS: Subjects’ mean (SD) age was 43 (8) years, weight 91 (13) kg, and BMI 28 (3) kg/m2. Fifty-one percent of subjects had ≥3 cardiovascular risk factors. One subject had ST depression on XT ECG, at least one positive A-ECG score for CAD, and documented CAD based on cardiology referral. While all other subjects, including those with fewer risk factors, higher aerobic fitness, and normal exercise ECGs, were classified as healthy by A-ECG, there was no trend for association between risk factors and any of 20 A-ECG parameters in the grouped data.

CONCLUSIONS: We have demonstrated that remote capture of a standard resting 12-lead ECG analyzed with advanced algorithms is a simple, time and cost-effective approach that offers the prospect of early identification of individuals potentially at risk for line-of-duty death from cardiovascular incidents.

A-38 Free Communication/Poster - Older Adults

MAY 30, 2012 7:30 AM - 12:30 PM

ROOM: Exhibit Hall

1416 Board #197 MAY 30 9:30 AM - 11:00 AM

The Practice of Physical Exercise is a Modifiable Factor for Urge Urinary Incontinence in Older

Giovana Zarpellon Mazo, Virtuoso Franck Janeisa. UDESC, Florianópolis, Brazil.

(No relationships reported)

The symptoms of urinary urgency tend to increase with advancing age. The literature suggests a number of risk factors that can be modified by an active lifestyle through healthy eating and physical exercise.

PURPOSE: To examine the modifiable risk factors of urge urinary incontinence (UUI) in older women.

METHODS: The sample consisted of 200 elderly (60 years or older) with a mean age of 69,06 ± 6,26 years. We identified the presence of UUI and behavioral risk factors (consumption of coffee, alcohol consumption, smoking and presence of constipation). Was also applied to the Domain 4 for International Physical Activity Questionnaire (IPAQ) to identify the level of physical activity and measured body mass index and waist circumference. The data were processed using descriptive and inferential statistics with a significance level of 5%.

RESULTS: The prevalence of UUI in the sample was 15,5%. Among the modifiable factors, only the level of physical activity was associated with the occurrence of UUI, and the physical exercise appeared as a protective factor among very active women (OR = 0,288) and less active (OR = 0,356).

CONCLUSIONS: The symptoms of urgency can be softened by regular physical exercise. Through a healthy lifestyle can minimize a number of modifiable factors in the genesis of urge urinary incontinence.

1417 Board #198 MAY 30 9:30 AM - 11:00 AM

Intermuscular Adipose Tissue Change is Related to Improved Physical Performance in Older Adults

Adam J. Santanasto, Anne B. Newman, Robert M. Boudreau, Mark A. Newman, Bret H. Goodpaster, Nancy W. Glynn. University of Pittsburgh, Pittsburgh, PA.

(No relationships reported)

Obesity exacerbates age-related declines in physical function, but there is controversy about the whether older adults should lose weight and whether weight or particular aspects of body composition should be targeted.

PURPOSE: To determine the relationship between changes in body composition and physical function in older adults, who participated in a 1 yr intervention study of physical activity + weight loss (PA+WL) compared to physical activity (PA).

METHODS: Thirty-six overweight to moderately obese (body mass index (BMI) 28-39.9 kg/m2) sedentary older adults mean age 70.3±5.9 yrs were randomized (GRP) to a 12 month program. PA program had a goal of 150 min/wk treadmill walking plus lower limb resistance and balance. The PA+WL group also underwent a weight loss intervention promoting 7% weight loss. Calorie and fat gram goals were based on the Diabetes Prevention Program. At baseline (BL) and 12-months (12) computerized tomography (CT) for intermuscular adipose tissue (IMAT) area of the thigh, visceral adipose tissue (VAT) area, thigh muscle area, muscle density, an indirect measure of intramuscular fat, in Hounsfield units (HU), subcutaneous adipose tissue area , BMI and short physical performance battery (SPPB) were assessed. The relationship between concurrent changes in body composition and physical function were determined using multivariate linear regression. Intervention groups were combined in these analyses.

RESULTS: SPPB score increased by an average of 0.53 ± 1.52 in both groups combined. Changes (BL-12) in several fat depots were associated with change in function. Change in IMAT was significantly related to change in SPPB after adjusting for BL IMAT, GRP and BL SPPB score, βadj = -0.29, SE =0.12, p=0.02; this was not explained by adjustment for change in BMI (βadj-bmi = -0.24, SE =0.12, p=0.05). HU and VAT were each associated with change in SPPB after adjusting for GRP and BL HU or VAT (p=0.06 and 0.05), respectively but these associations were completely attenuated by change in BMI. Change in muscle area was not related to change in SPPB.

CONCLUSION: Change in IMAT was the body composition parameter most strongly related to change in function. Interventions that target IMAT in older adults may be of particular importance for improving function.

Supported by CDC cooperative agreement 1 U48 DP000025

1418 Board #199 MAY 30 9:30 AM - 11:00 AM

Association Between Sociodemographic, Neighborhood Environment And Domain Of Physical Activity Among Middle-aged And Elderly Japanese.

Yoshinobu Saito1, Yuko Oguma2, Shigeru Inoue3, Ayumi Tanaka4, Yoshitaka Kobori4. 1Fujisawa City Health and Medical Center, Fujisawa, Kanagawa, Japan. 2Keio University, Yokohama, Japan. 3Tokyo Medical University, Shinjuku, Japan. 4Fujisawa City Health and Medical Center, Fujisawa, Japan.

(No relationships reported)

Recent studies suggested the importance of neighborhood environment as physical activity determinants. However, few studies have been investigated in Japan.

PURPOSE: To examine the association of moderate-to-vigorous intensity leisure-time physical activity (LTPA), walking for recreation, walking for transportation with sociodemographic/health and neighborhood environmental factors among community dwelling middle-aged and elderly Japanese.

METHODS: The study included 2,449 adults aged 40-69 years living in Fujisawa city, located at 40km southwest of Tokyo, who had taken the Specific Health Checkups in 2009 and responded to the additional survey by mail in 2010. Sociodemographic/health factors (gender, age, education, working, economic, children in household, self-rated health, orthopedic disorders, body mass index), the long version of International Physical Activity Questionnaire and its Environmental Module were obtained. The adjusted odds ratios (OR) of high levels of LTPA, walking for active transportation/recreation were calculated in relation to these sociodemographic/health and neighborhood environmental factors using multiple logistic regression models.

RESULTS: Higher age and good self-rated health were significantly associated with high level of each physical activity outcome (P<0.05). Having work (OR=0.74), presence of children in household (OR=0.65), higher educational attainment (OR=1.58), higher economic status (OR=1.38), good access to recreational facilities (OR=1.26), and not owing household motor vehicles (OR=0.65) were associated with longer LTPA time. Women (OR=0.68), having work (OR=0.52), presence of children in household (OR=0.62), not having strong pain by orthopedic disorders (OR=1.58), seeing people being active (OR=1.41), and good aesthetics (OR=1.37) were associated with longer walking time for recreation. Higher economic status (OR=0.81), not owing household motor vehicles (OR=2.18), good access to shops (OR=1.35), and presence of sidewalks (OR=1.24) were associated with longer walking time for transportation.

CONCLUSION: The results suggest that different individual and neighborhood environmental variables are associated with different physical activity outcomes among community-dwelling middle-aged and elderly Japanese.

1419 Board #200 MAY 30 9:30 AM - 11:00 AM

Assessment of a Physical Activity Program in Low-Income Seniors Living in Independent Community Residences

Leticia Malavasi, Cathy Inouye. California State University, East Bay, Hayward, CA. (Sponsor: Calvin Caplan, FACSM)

(No relationships reported)

The Nutrition and Fitness program offered through the Senior Support Program of the Tri-Valley provides on-site free of charge physical activity classes for low-income seniors living in community residences. Their primary goal is to improve the quality of life in part by helping participants maintain their independence, of which fitness is a pivotal component. The following analysis of senior fitness assessments was conducted on data gathered from five community sites over a 9-18 month time period. Based on those findings, recommendations for programmatic changes will be implemented with oversight from the investigators and following the securement of grant funding.

PURPOSE: To analyze the effect of an on-site physical activity program for independent-living seniors and implement subsequent programmatic changes.

METHODS: Forty-two female (75±4.6 years) and 9 male (81±4 years) participants attended two 60-minute exercise classes per week. Classes were led by 3 different instructors and followed a program template that included a 5-10 min warm-up, 20-30 min aerobic, strength, and balance exercises, 5-10 min cool-down, and 5 min relaxation session. Pre to post-fitness assessments of balance, strength, muscular endurance, agility, and flexibility were analyzed using dependent t-tests. Post-test scores between sites was analyzed using independent t-tests.

RESULTS: Pre to post-strength (bicep curls/30 sec, 16.7±4.9 vs. 19.8±5.1 repetitions, p<0.05) and agility (8’ Up & Go, 7.3±2.2 vs. 7.8±2.6 seconds, p=0.05) increased when data was combined for all sites. Comparison of post-strength scores between one versus multi-instructor sites was also significant (22.6±5.8 vs. 18.8±4.6 repetitions, p=0.02)

CONCLUSION: The lack of change in several fitness parameters and on-site observation of individual classes has led to a number of programmatic recommendations including standardization of instruction, implementation of balance courses, incorporation of activities that utilize large muscle groups, and closer monitoring of exercise intensity.

1420 Board #201 MAY 30 9:30 AM - 11:00 AM

Habitual Physical Activity is Associated With Cognitive Function And The Risk Factors For Hip Fracture In Older Adults

Hyuntae Park1, Youchan Kwon2, Eunhee Kim2, Jinkee Park2, Sangkab Park2. 1National Center for Geriatrics and Gerontology, Aichi, Japan. 2Dong-A University, Busan, Korea, Republic of.

(No relationships reported)

Regular physical activity is an important component of a healthy lifestyle, conferring many physical health benefits, including protection against, osteoporosis, sarcopenia and cognitive impairment. However, only a few study has reported actual daily physical activity levels in relation to the reduction of cognitive function and risk factors of hip fracture.

PURPOSE: In this prospective study, we examined the association between baseline physical activity and cognitive decline and risk factor for hip fracture in order adults.

METHODS: Using the KMMSE, we tested the cognitive function, and participants reported all previous hip fracture with circumstances leading to fracture between baseline and 2 year follow-up. Using an electronic accelerometer calculated total number of steps during a month. Body sway was measured by using the Dynamic Posturography (AMTI OR 6-7-2000, Massachusetts, U.S.A.). Each subject performed 10 m maximal walk speed, maximal step length, one-legged-stand, 40cm step up and down test, and functional reach and trunk flexion tests.

RESULTS: The risk of cognitive decline in the lowest two quartiles (lowest and low activity group, with respectively means of counts of about 3400 and 6800 steps/day) was 1.6-2.6 times higher than in the top quartile (highest group, taking means of about >10000 steps/day). We founded significant lineal associations between physical activity levels and the gait velocity.

Compared with a lowest and low activity group, older adults reporting a high activity group (> 7000 steps/day) had a significantly lower risk of hip fractures such as a lateral body sway, functional reach and maximal step length.

CONCLUSIONS: In this study, we suggest that to improve cognitive decline and the risks of hip fracture older adults should be encouraged to undertake > 7000 steps/day.

1421 Board #202 MAY 30 9:30 AM - 11:00 AM

Evaluating Moderate Intensity Walking With A Hip-worn Accelerometer In Elders

Todd Manini, Jeff Knaggs. University of Florida, Gainesville, FL.

(No relationships reported)

PURPOSE: The National Health and Nutrition Examination Survey (NHANES) uses activity counts from accelerometers to objectively categorize the population’s physical activity level. It is unclear activity counts correspond to metabolic effort in older adults. This study set out to examine the association between activity counts derived from a hip-worn accelerometer and metabolic effort in older adults performing usual and rapid paced walking.

METHODS: Forty-five community-dwelling older adults (70-90 years) performed long-distance walking tasks at a self-selected usual and rapid pace in a laboratory setting. During each walking test, participants wore a portable metabolic unit to measure pulmonary gas exchange and a hip-worn accelerometer (Actigraph GT1M) to measure activity counts. Metabolic equivalents (MET) were calculated as ventilation of oxygen (VO2) / 3.5 ml·kg-1·min-1. Values that surpassed 3.0 METs and 2020 counts/min were considered walking at or greater than moderate intensity. Participants were categorized as a “slow walker” if they had a usual pace < 1.0 meters/sec_ a common cutoff used to designate an older person with functional impairments.

RESULTS: Activity counts recorded while walking at rapid (r = 0.62, p < 0.01), but not usual pace (r = 0.24, p = 0.11) was significantly associated with METs. Slow walkers attained only half the amount of activity counts during each walk condition (usual: 1125 ± 132 vs. 2242 ± 121; rapid: 1534 ± 214 vs. 3117 ± 219 counts/min, p < 0.01) while at the same time achieving between 82-90% of MET level of individuals who walked ≥ 1.0 m/sec. When using the NANES cutoff of 2020 counts/min, 75% of slow walkers were misclassified as not achieving a moderate intensity MET level (18 out of 24 occurrences, p = 0.09). Walking above 1.0 m/sec misclassified 25% of the individuals as not achieving a moderate intensity MET level (13 out of 52 occurrences, p < 0.01).

CONCLUSIONS: Activity counts from a hip-worn accelerometer are marginally correlated with walking metabolic rate in older adults. Accelerometers might misclassify functionally impaired older adults with slow walking speed as not achieving the recommended physical activity intensity to produce health benefits. Additional research is needed to derive better accelerometer activity count thresholds for older adults.

1422 Board #203 MAY 30 9:30 AM - 11:00 AM

Exploring Physical Activity Patterns on Body Composition Phenotypes of Sarcopenia and Obesity in Older Adults

Mi-Ji Kim, Hunkyung Kim, Narumi Kojima. Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan.

(No relationships reported)

Along with sarcopenia, obesity is an important cause of the development of functional impairment and frailty in older adults. Physical inactivity or low physical activity (PA) has been reported to be independently associated with sarcopenia and obesity. However, little is known about the impact of PA patterns on body composition phenotypes based on sarcopenia and obesity in older adults.

PURPOSE: This study examined the association between objectively measured PA patterns and body composition phenotypes based on sarcopenia and obesity in frail older women.

METHODS: A cross-sectional analysis was conducted on 109 community-dwelling frail older women with a mean age of 80.3 years (SD = 2.7, range = 75-88). Measurements of appendicular skeletal muscle mass and body fat percentage were assessed by dual energy X-ray absorptiometry, which were used to characterize normal (N), sarcopenic (S), obese (O), and sarcopenic/obese (SO) body composition phenotypes. The objective assessment of physical activity was obtained for a 1-week period using a triaxial accelerometer (Activity Style pro). With intensity as sedentary (1.0-1.5 metabolic equivalent units, METs), light PA (1.6-2.9 METs), and moderate to vigorous PA (MVPA, ≥ 3 METs), average daily time spent being each PA was examined.

RESULTS: The O group was the most prevalent 36.7% in frail older women, followed by the S (29.4%), N (22.0%), and SO (11.9%) groups. On average, participants wore the accelerometer for 766.3 ± 102.8 min/day. Overall, participants spent 57.6% of their daily time being sedentary behavior, 39.7% in light PA, and 2.7% in MVPA. The time spent in sedentary behavior was significantly more in the O and SO groups than the normal group (p < 0.05). Time spent in light PA was similar between the O (292.7 ± 91.8 min/day) and SO (243.6 ± 88.1 min/day) groups but significantly less than the N (357.5 ± 97.1 min/day) group (p < 0.05), but not the S group. There was no difference in time spent in MVPA among body composition phenotypes.

CONCLUSIONS: These findings suggest that majority of daily time in frail older women spend being sedentary lifestyle, and obesity phenotypes are more strongly related to the light PA level, independent of sarcopenia.

1423 Board #204 MAY 30 9:30 AM - 11:00 AM

5-year Changes In Functional Fitness of Brazilian Elderly Women

Maressa P. Krause1, Hassan M. Elsangedy2, Kleverton Krinski2, Fredric L. Goss, FACSM3, Sergio G. DaSilva2. 1Universidade Tecnológica Federal do Parana, Curitiba, Brazil. 2Universidade Federal do Parana, Curitiba, Brazil. 3University of Pittsburgh, Pittsburgh, PA.

(No relationships reported)

Functional fitness tests have been used to evaluate functional components associated with physiological parameters, which are necessary for older adults to perform activities of daily living independently. Aging can decrease functional fitness; however, evidence had indicated that exercise can reverse or attenuate this process.

PURPOSE: To verify changes on functional fitness of Brazilian elderly women, classified according to their physical activity status.

METHODS: On 2011, 78 women (mean: 73.2yrs) were evaluated with the same procedures of the first assessment, conducted on 2005-2006. Functional fitness components were evaluated by body mass index (BMI), cardiorespiratory fitness (CRF) using the 6-min walk test (6WT), flexibility using the chair sit-and-reach (CSR), strength using the arm curl (AC) and chair stand (CS), and dynamic balance and agility using the 8-ft up-and-go (8ft). Regular physical activity was evaluated by the Modified Baecke Questionnaire for older adults.

RESULTS: MANOVA determined time (F1,140=5.950; p<0.05) and groups main effect (F2,140=1.850; p0.05). There was a significant decrease on CRF over time in the active group (-9.2%); on muscle strength of upper body in the inactive (-35.2%) and active (-25.5%) groups; and, on muscle strength of lower body in the active group (-11.8%). Active women had a greater CRF (480.3±72.3m) than inactive women (409.5±119.9m) on the second assessment; also, active women had a better performance on the dynamic balance and agility test (5.86±098s) than inactive women (7.11±1.41s) on the first assessment, and on the second assessment (5.91±1.01s; and 7.50±2.80s, respectively) (all p<0.05).

CONCLUSIONS: Active elderly women showed a lower decrease on functional fitness than inactive women. Also, the active group had a better cardiorespiratory fitness and dynamic balance and agility than inactive women. These results highlight the benefits of regular exercise practice on functional fitness of older women.

1424 Board #205 MAY 30 9:30 AM - 11:00 AM

Recruitment Of Frail Older Adults Residing At Assisted Living Facilities Into An Exercise-nutrition Field Trial.

Michael P. Corcoran, Miriam E. Nelson, FACSM, Roger A. Fielding, Jennifer M. Sacheck, Sara C. Folta. Tufts University, Boston, MA.

(No relationships reported)

Exercise-nutrition programs designed to improve functional mobility in older adults are essential. Researching the efficacy of such programs in this population presents unique challenges, particularly with respect to study recruitment.

PURPOSE: To describe the challenges to recruitment of mobility limited older adults in assisted living facilities, and to compare the relative resource requirements and efficacy of two recruitment methods.

METHODS: Fifteen facilities in the greater Boston area were selected for this study. Residents were eligible if they were ≥65 years old, exhibited moderate mobility limitations, no severe memory impairment, and were not severely obese. At eight sites, “informational sessions”, which were heavily advertised through facility channels and included a brief presentation about the study, were the only type of recruitment activity conducted. At seven sites, informational sessions were preceded by a researcher attending an activity offered by the facility (“activity attendance”), such as dances and bingo games. Staff time for both recruitment types was documented, as was number screened, number eligible, and number enrolled (eligible minus withdrawals) for each facility.

RESULTS: Severe memory impairment (38.7%) followed by severe mobility limitations (14.5%) were the leading causes of ineligibility, indicating the population was more functionally limited than anticipated. Other challenges included apathy due to institutionalized status and high facility staff turnover making it difficult to obtain support for recruitment activities. The addition of activity attendance resulted in 4-11 additional research staff hours spent on recruitment per facility. Compared with an informational session alone, attendance at activities resulted in better targeting: slightly more of those screened were eligible (51.7% vs 43.9% respectively), and significantly more of those who were eligible remained in the study (70.0% vs 12.0%, p<0.01). The two leading causes of withdrawal overall were loss of interest and/or time constraints (40%) and illness (40%).

CONCLUSION: Increased interaction with older adults residing at assisted living facilities results in improved recruitment and retention in an exercise-nutrition study, and is likely worth the additional resources required.

1425 Board #206 MAY 30 9:30 AM - 11:00 AM

Prediction of Maximal Oxygen Pulse without Exercise Testing in Older Adults

Geraldo A. Maranhao Neto1, Ricardo B. Oliveira2, Paulo T.V. Farinatti2, Jonathan N. Myers, FACSM3. 1Gama Filho University, Rio de Janeiro, Brazil. 2Rio de Janeiro State University, Rio de Janeiro, Brazil. 3Palo Alto/Stanford University, Palo Alto, CA. (Sponsor: Jonathan N. Myers, FACSM)

(No relationships reported)

Maximal Oxygen Pulse (O2Pmax) has been considered a surrogate for cardiovascular function and has demonstrated to be an independent predictor of all cause mortality in healthy subjects and patients with cardiovascular disease. Although readily available from cardiopulmonary exercise testing, the O2Pmax depends on maximal volitional effort which may limit its measurement in older subjects with physical limitations.

PURPOSE: The aim of this study was to develop a model to estimate the maximal O2 pulse without exercise in elderly subjects.

METHODS: The equation was derived from a sample of 67 older adults (69.4 ± 7.1 yrs; 41 men) through a hierarchical linear regression that included body weight, Veterans Specific Activity Questionnaire (VSAQ) score, gender, beta-blocker usage and resting heart rate. The model was cross-validated against an independent sample (67.7 ± 6.4 years; N=30; 17 men). In order to detect the classification accuracy of the model, the estimated and actual O2Pmax were ranked in tertiles and treated by the gama (γ) nonparametric correlation in both groups (validation and cross-validation).

RESULTS: The model resulted in a R2=0.83 and standard error of estimate=1.68ml/beat. The comparison of the estimated against the actual O2Pmax in the independent sample was r=0.80. A high probability for the model to rank the values in the same tertile in both validation and cross-validation groups was observed (γ=0.98; γ=0.92, respectively).

CONCLUSION: Our findings suggest that O2Pmax can be estimated with reasonable precision without aerobic effort, being an alternative option for older subjects not able to perform maximal exercise testing.

Commercial Relationship: Paulo T.V. Farinatti, CNPq Research Grant Recipient.

1426 Board #207 MAY 30 9:30 AM - 11:00 AM

Group Pilates Program And Muscular Strength And Endurance Among Elderly Woman

Ina Shaw1, Marinda Fourie2, Gertrude M. Gildenhuys2, Brandon S. Shaw2, Gregory A. Brown, FACSM3. 1Monash South Africa, Johannesburg, South Africa. 2Tshwane University of Technology, Pretoria, South Africa. 3University of Nebraska at Kearney, Kearney, NE.

(No relationships reported)

Muscular strength and peak power output substantially decline with age and can lead to impairments in neuromuscular function, degradation of the hormonal system and intrinsic factors such as age-related alterations in calcium homeostasis.

PURPOSE: The purpose was to determine the effects of mat Pilates on muscular strength using dumbbell biceps curls and squats, and muscular endurance using chair squats in elderly woman.

METHODS: Fifty sedentary, apparently healthy females aged 60 and older were randomly assigned into a control (CON, n = 25) or an intervention (INT, n = 25) group. The INT took part in an eight-week supervised progressive Pilates exercise program with sessions being conducted by a qualified Pilates instructor. The programme consisted of three non-consecutive 60 minute sessions that were repeated for the eight-week period only increasing in intensity. All sessions commenced with breathing, followed by a flowing system from standing, to sitting, to lying down exercises and ended in the rest position. The CON did not take part in any structured exercises throughout the eight-week period and were instructed to continue their usual activities.

RESULTS: Significant (p ≤ 0.05) improvements in upper-body muscular strength (19.12 ± 5.13 repetitions (reps) - 27.84 ± 5.68 reps; p = 0.000), lower-body muscular strength (13.24 ± 3.23 reps - 7.52 ± 3.81 reps; p = 0.000) and muscular endurance (24.48 ± 11.62 reps to 44.16 ± 18.97 reps; p = 0.000) were observed following Pilates training.

CONCLUSIONS: Programs utilizing Pilates can improve muscular strength and endurance in the elderly and may assist in decreasing dependency and possibly lead to an improved quality of life in the elderly.

1427 Board #208 MAY 30 9:30 AM - 11:00 AM

Past Year Leisure Moderate to Vigorous Physical Activity is Associated with Calcaneal Bone Density and Stiffness in Perimenopausal and Postmenopausal Women

Heather C. Medema-Johnson1, Kathleen F. Janz, FACSM2. 1St. Ambrose University, Davenport, IA. 2University of Iowa, Iowa City, IA.

(No relationships reported)

Along with calcium intake, weight-bearing moderate to vigorous physical activity (MVPA) has been shown to decrease the rate of bone loss in peri- and postmenopausal women. However, the importance of domain- and time-specific characteristics of MVPA to bone remains unclear.

PURPOSE: To evaluate relationships among domain-specific long term (past year) and short term (past week) MVPA, calcium and vitamin D intakes, and calcaneal BMD and stiffness (QUI).

METHODS: For this cross-sectional-study, 87 peri- and postmenopausal women (43-65 years) completed demographic/health questionnaires and the Block calcium and vitamin D screener. Participants wore a NL-1000 pedometer for one week to assess short-term MVPA (total steps/day and MVPA-min/day). Past year MVPA, past year leisure MVPA, past year occupational/household MVPA, past week leisure MVPA, and past week occupational/household MVPA were assessed with the Modifiable Activity Questionnaire. Calcaneal BMD and QUI were measured with a Sahara Quantitative Ultrasound (Hologic, Inc.). Pearson correlation coefficients and stepwise linear regression analyses were used to examine the relationships among measures of MVPA, calcium and vitamin D intakes, calcaneal BMD and QUI. Income and education entered as control variables in stepwise regression analyses.

RESULTS: Bivariate analyses revealed significant relationships between BMD and pedometer-measured MVPA-min/day (r = 0.23, p < 0.05). Past year MVPA (r = .25, p < 0.05), and past year leisure MVPA (r = 0.35, p < 0.01) measured via survey were associated with BMD. Significant relationships were found between QUI and past year MVPA (r = 0.23, p < 0.05) and past year leisure MVPA (r = 0.34, p < 0.01). For stepwise linear regression analyses, income, education, and past year leisure MVPA entered the models, collectively explaining 19% and 18% of the variance in BMD and QUI.

CONCLUSION: Past year MVPA performed in the leisure domain is associated with calcaneal BMD and QUI in peri- and postmenopausal women. Our results suggest the value of long-term measures of PA when considering bone health and the likelihood that women engage in more osteogenic activity during leisure when compared to other domains. These findings can be used to support future interventions and the continued need for multi-dimensional PA measures.

1428 Board #209