B-17 Thematic Poster - Nutritional Antioxidants and Oxidative Stress: JUNE 1, 2011 1:00 PM - 3:00 PM: ROOM: 404
PURPOSE: To determine whether blueberry consumption (250 g per day for 6-wks) and 375 g 1-h prior to a 2.5-h run counters oxidative stress and inflammation.
METHODS: Twenty-five subjects were recruited and randomized into blueberry (BB) (N=13) or control (CON) (N=12) groups. Blood, muscle, and urine samples were obtained pre-exercise and immediately post-exercise, and blood and urine 1- h post-exercise. Blood was examined for oxidative stress (F2-isoprostanes, FRAP, and GSH) and inflammation (cortisol, cytokines, and lymphocyte subset cell counts). Muscle biopsy samples were examined for glycogen content and NFkB expression. Urine samples were tested for modified bases of DNA (8-OHDG) and RNA (5-OHMU). A 2 treatment x 3 time (blood and urine measures) and x 2 time (muscle measures) repeated measures ANOVA was used to test main effects.
RESULTS: When comparing BB versus CON, post-exercise F2-isoprostanes and
5-OHMU were lower (treatment effects, P=0.016 and P=0.028, respectively), and plasma IL-10 and blood natural killer (NK) cell counts higher (P=0.045 and P=0.003, respectively). Changes in all other markers did not differ from CON.
CONCLUSION: This study indicates that daily blueberry consumption over six weeks coupled with acute ingestion reduces exercise-induced oxidative stress and inflammation and increases circulating numbers of NK cells.
Supported by a grant from the North American Blueberry Council