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F-21 Free Communication/Poster - Clinical Exercise Physiology 4 (Clinical Exercise Physiology Association): JUNE 3, 2011 1:00 PM - 6:00 PM: ROOM: Hall B

Combined Influence of Hormone Therapy and Eccentric Exercise on Myostatin-Related Gene Expression in Postmenopausal Women: 2706Board #5 June 3 2:00 PM - 3:30 PM

Dieli, Christina; Spektor, Tanya M.; Rice, Judd C.; Sattler, Fred R.; Schroeder, E. Todd FACSM

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Medicine & Science in Sports & Exercise: May 2011 - Volume 43 - Issue 5 - p 754-755
doi: 10.1249/01.MSS.0000402098.05102.33
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PURPOSE. Potential effects of hormone therapy (HT) on skeletal muscle are not well understood. We sought to evaluate changes in gene expression of myostatin-related factors in postmenopausal women taking HT and not taking HT following eccentric resistance exercise.

METHODS. Fourteen postmenopausal women participated including 6 control women not using HT (59 ± 4 yrs, 63 ± 17 kg) and 8 women using HT (59 ± 4 yrs, 89 ± 24 kg). Participants performed 10 sets of 10 maximal eccentric repetitions of single-leg extension on a Cybex dynamometer. Muscle biopsies from the vastus lateralis were obtained from the exercised leg at baseline and 4 hours after the exercise bout. Gene expression was determined using RT-PCR for myostatin, ActRIIb (activin receptor IIb), follistatin, FLRG (follistatin-related gene), FSTL3 (follistatin-like-3) and GASP-1 (GDF-serum associated protein-1).

RESULTS. At rest, the HT group expressed lower levels of myostatin and ActRIIb and higher levels of follistatin, FLRG, FSTL3 and GASP-1 (P < 0.05). In response to eccentric exercise, myostatin and ActRIIb significantly decreased (P < 0.05) and follistatin, FLRG, FSTL3 and GASP-1 significantly increased in both groups (P < 0.05). Significantly greater changes in gene expression of all genes occurred in the HT group than in the control group after exercise (P < 0.05).

CONCLUSIONS. These data suggest that postmenopausal women using HT express greater myostatin-related gene expression, which may reflect a mechanism by which estrogen influences the preservation of muscle mass. Further, postmenopausal women using HT experienced a profoundly greater myostatin-related response to maximal eccentric exercise.

Funding: NCRR GCRC M0I RR000043

© 2011 American College of Sports Medicine