E-27 Free Communication/Poster - Environment and Applied Physiology: JUNE 3, 2011 7:30 AM - 12:30 PM: ROOM: Hall B
The effective environmental disinfection of locker rooms, training rooms, and buses is an important and underexplored approach for the control and reduction of MRSA infections. Multiple studies have shown that environmental contamination plays a significant role in the transmission of MRSA, which can survive on surfaces for prolonged periods of time. Furthermore, it has been demonstrated that the reoccupation of rooms previously inhabited by an individual infected by MRSA significantly increases the probability of the new occupant becoming infected. This coupled with spray and wipe applications, which frequently do not deliver the appropriate amount of disinfectant or the proper exposure time, can lead to the dissemination of localized contamination into a wider area. Because of these issues, the efficacy of novel application methods for the decontamination of such environments is desperately needed. We present here the application of such a system, which is capable of delivering a disinfectant to all surfaces in a room accessible to free airflow, and, through automation, is able to eliminate problems associated with user error, such as improper amounts of disinfectant being used, insufficient exposure time, or missed spots.
PURPOSE: To test the efficacy of an automatic room and vehicle decontamination system on MRSA killing in various locations, including a locker room, training room, and bus.
METHODS: Using a sterile inoculating loop, an isolated colony of USA100 MRSA was inoculated into a flask Trypric Soy Broth (TSB). In a BSC, a 10 μl aliquot of the test culture was added to the non-frosted end of a sterile glass slide using a calibrated micro pipette. The inoculums were spread over a 1" × 1" surface of the slide using a sterile pipette tip. The slides were placed at various locations throughout the room. Using a Zimek Touch-Free Rapid Decontamination System, The room was treated for 76 minutes (25 minute misting, 20 minute dwell, and 31 minute Zvac).
RESULTS: An average kill of 99.9996% was observed with no cells recovered. The untreated controls averaged 2,820,000 CFU/carrier.
CONCLUSIONS: The automated decontamination process effectively eradicated MRSA from multiple athletic facilities that have been implicit in transmission of cutaneous infections.