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C-26 Free Communication/Poster - Nutritional Interventions: MAY 31, 2007 7: 30 AM 12: 30 PM ROOM: Hall E

A 6-Month Follow-Up of Mice Fed a Low-Carbohydrate High-Fat Diet


Board #57 May 31 8:00 AM 9:30 AM

Mjosund, Katja Peltola; Ahola, Sofia; Tyynismaa, Henna; Lappalainen, Ilse; Suomalainen, Anu

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Medicine & Science in Sports & Exercise: May 2007 - Volume 39 - Issue 5 - p S292
doi: 10.1249/01.mss.0000274128.23981.55
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Low-carbohydrate diets are extremely popular. Moreover, some evidence indicates, that a ketogenic (low carbohydrate, high-fat) diet may provide neuroprotection in certain neurological conditions such as Parkinson's disease, Alzheimer's disease or brain trauma.

PURPOSE AND METHODS: To study long-term consequences of a low-carbohydrate, high-fat diet on exercise performance, we investigated 10 (5 male) C57BL/6J mice and 10 (5 male) littermate controls fed either a ketogenic high-fat diet (HF; fat 89.5, carbohydrates 0.1 and protein 10.4 kcal%, respectively) or a control diet (C; fat 11.5, carbohydrates 78.1 and protein 10.4 kcal%, respectively). The diet was introduced at the age of 4 months and the exercise capacity was studied by treadmill running test and a Rotarod test at month 0, 3 and 6. The treadmill test was started by a velocity of 7m/min and the velocity was increased every 2 minutes by 2m/min until the mouse could not keep the pace. The results were expressed in seconds. The time the mouse could run on an accelerating Rotarod bar was also expressed in seconds. In both tests, the best of 3 attempts was chosen for analysis. The body weight was monitored every 1–3 weeks during the study period.

RESULTS: At the baseline, the body weight was similar in both groups. After 6 months, the body weight was increased by an average of 111 % in the FD mice whereas in the control mice the average increase was 33%.

The treadmill performance was similar in both groups at baseline (FD 652 ± 162 sec vs 594 ± 122) but after 6 months, the FD mice performed clearly worse (257 ± 62 sec vs 408 ± 79 sec). However, when related to body weight, the treadmill performance at 6 months was similar in both groups. In Rotarod performance, there was a difference between groups both at baseline and at 6 months (96 ± 56 sec and 54 ± 16 sec in FD; 131 ± 57 sec and 123 ± 33 sec in C). However, the percentual decrease in Rotarod performance with time was similar in both groups.

CONCLUSIONS: A high-fat, low carbohydrate ketogenic diet does not per se influence exercise capacity, but the massive increase in body weight negatively affects physical performance.

© 2007 American College of Sports Medicine