Institutional members access full text with Ovid®

Share this article on:

Endurance Exercise Attenuates Postprandial Whole-Body Leucine Balance in Trained Men

MAZZULLA, MICHAEL1; PAREL, JUSTIN T.2; BEALS, JOSEPH W.3; VAN VLIET, STEPHAN2; ABOU SAWAN, SIDNEY1; WEST, DANIEL W. D.1; PALUSKA, SCOTT A.4; ULANOV, ALEXANDER V.5; MOORE, DANIEL R.1; BURD, NICHOLAS A.2,3

Medicine & Science in Sports & Exercise: December 2017 - Volume 49 - Issue 12 - p 2585–2592
doi: 10.1249/MSS.0000000000001394
Applied Sciences

Purpose Endurance exercise increases indices of small intestinal damage and leucine oxidation, which may attenuate dietary amino acid appearance and postprandial leucine balance during postexercise recovery. Therefore, the purpose of this study was to examine the effect of an acute bout of endurance exercise on postprandial leucine kinetics and net leucine balance.

Methods In a crossover design, seven trained young men (age = 25.6 ± 2.3 yr; V˙O2peak = 61.4 ± 2.9 mL·kg−1·min−1; mean ± SEM) received a primed constant infusion of L-[1-13C]leucine before and after ingesting a mixed macronutrient meal containing 18 g whole egg protein intrinsically labeled with L-[5,5,5-2H3]leucine, 17 g fat, and 60 g carbohydrate at rest and after 60 min of treadmill running at 70% V˙O2peak.

Results Plasma intestinal fatty acid binding protein concentrations and leucine oxidation both increased (P < 0.01) to peaks that were ~2.5-fold above baseline values during exercise with a concomitant decrease (P < 0.01) in nonoxidative leucine disposal. Meal ingestion attenuated (P < 0.01) endogenous leucine rates of appearance at rest and after exercise. There were no differences (both, P > 0.05) in dietary leucine appearance rates or in the amount of dietary protein-derived leucine that appeared into circulation over the 5-h postprandial period at rest and after exercise (62% ± 2% and 63% ± 2%, respectively). Leucine balance over the 5-h postprandial period was positive (P < 0.01) in both conditions but was negative (P < 0.01) during the exercise trial after accounting for exercise-induced leucine oxidation.

Conclusions We demonstrate that endurance exercise does not modulate dietary leucine availability from a mixed meal but attenuates postprandial whole-body leucine balance in trained young men.

Supplemental digital content is available in the text.

1Faculty of Kinesiology and Physical Education, University of Toronto, Toronto, Ontario, CANADA; 2Department of Kinesiology and Community Health, University of Illinois at Urbana–Champaign, Champaign, IL; 3Division of Nutritional Sciences, University of Illinois at Urbana–Champaign, Champaign, IL; 4Department of Family Medicine, University of Illinois at Urbana–Champaign, Champaign, IL; and 5Roy J. Carver Biotechnology Center, University of Illinois at Urbana–Champaign, Champaign, IL

Address for correspondence: Nicholas A. Burd, Ph.D., Department of Kinesiology and Community Health, University of Illinois at Urbana–Champaign, 352 Louise Freer Hall, 906 S. Goodwin Avenue, Urbana, IL 61801; E-mail: naburd@illinois.edu.

M. M. and J. T. P. contributed equally to this work.

Submitted for publication May 2017.

Accepted for publication July 2017.

Supplemental digital content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal’s Web site (www.acsm-msse.org).

© 2017 American College of Sports Medicine