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Exercise Training, NADPH Oxidase p22phox Gene Polymorphisms, and Hypertension


Medicine & Science in Sports & Exercise: July 2009 - Volume 41 - Issue 7 - p 1421-1428
doi: 10.1249/MSS.0b013e318199cee8
Basic Sciences

Introduction: Oxidative stress that is mediated through NADPH oxidase activity plays a role in the pathology of hypertension, and aerobic exercise training reduces NADPH oxidase activity. The involvement of genetic variation in the p22phox (CYBA) subunit genes in individual oxidative stress responses to aerobic exercise training has yet to be examined in Pre and Stage 1 hypertensives.

Methods: Ninety-four sedentary Pre and Stage 1 hypertensive adults underwent 6 months of aerobic exercise training at a level of 70% V˙O2max to determine whether the CYBA polymorphisms, C242T and A640G, were associated with changes in urinary 8-iso-prostaglandin F (8-iso-PGF), urinary nitric oxide metabolites (NOx), and plasma total antioxidant capacity (TAC).

Results: Demographic and subject characteristics were similar among genotype groups for both polymorphisms. At baseline, a significant (P = 0.03) difference among the C2424T genotype groups in 8-iso-PGF levels was detected, with the TT homozygotes having the lowest levels and the CC homozygotes having the highest levels. However, no differences were found at baseline between the A640G genotype groups. After 6 months of aerobic exercise training, there was a significant increase in V˙O2max (P < 0.0001) in the entire study population. In addition, there were significant increases in both urinary 8-iso-PGF (P = 0.002) and plasma TAC (P=0.03) levels and a significant decrease in endogenous urinary NOx (P < 0.0001). Overall, aerobic exercise training elicited no significant differences among genotype groups in either CYBA variant for any of the oxidative stress variables.

Conclusions: We found that compared with CYBA polymorphisms C242T and A640G, it was aerobic exercise training that had the greatest influence on the selected biomarkers; furthermore, our results suggest that the C242T CYBA variant influences baseline levels of urinary 8-iso-PGF but not the aerobic exercise-induced responses.

1Hypertension, Molecular and Applied Physiology Laboratory, Department of Kinesiology, Temple University, Philadelphia, PA; 2Department of Kinesiology, School of Public Health, University of Maryland, College Park, MD; 3Cardiology Branch, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, MD; 4Department of Internal Medicine, Section on Gerontology and Geriatric Medicine, Wake Forest University School of Medicine, Winston Salem, NC; 5Clinical Nutrition and Metabolism, Department of Public Health and Caring Sciences, Faculty of Medicine, Uppsala University, Uppsala, SWEDEN; and 6Department of Human Genetics, University of Pittsburgh Graduate School of Public Health, Pittsburgh, PA

Address for correspondence: Deborah L. Feairheller, B.S., Department of Kinesiology, Temple University, 16 Pearson Hall, 1800 N Broad St, Philadelphia, PA 19122; E-mail:

Submitted for publication July 2008.

Accepted for publication December 2008.

©2009The American College of Sports Medicine