Carbonic anhydrase III and collagen markers in serum following cross-country skiing

Abstract

TAKALA, T. E. S., J. J. VUORI, P. J. RAHKILA, E. O. HAKALA, J. A. KARPAKKA, M. J. ALEN, Y. S. ORAVA, and H. K. VÄÄNÄNEN. Carbonic anhydrase III and collagen markers in serum following cross-country skiing. Med. Sci. Sports Exerc., Vol. 21, No. 5, pp. 593–597, 1989. Protein leakage from type I skeletal muscle cells and collagen synthesizing cells were studied during a 24-h crosscountry skiing relay in eight men using serum carbonic anhydrase III (S-CA III) and serum galactosylhydroxylysyl glucosyltransferase (S-GGT) as the respective specific marker proteins. Serum aminoterminal propeptide of type III procollagen (S-Pro(III)-N-P) was used as an indicator of type III collagen synthesis and serum creatine kinase (S-CK) as a nonspecific muscular marker. S-CK, S-CA III, and S-GGT were increased immediately following the skiing relay by 870%, 640%, and 40%, respectively. No significant change was observed in the concentration of S-Pro(III)-N-P following the exercise. A high positive correlation (r_s = 0.92, P < 0.01) was found between the increase in S-CA III and S-CK, whereas no correlation was observed between these muscular markers and S-GGT. The results suggest leakage from type I skeletal muscle cells and collagen synthesizing cells as a consequence of prolonged cross-country skiing, but there seems to exist no dependence between acute responses in muscular and connective tissue cells to exercise.