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Exercise Improves Fat Metabolism in Muscle But Does Not Increase 24-h Fat Oxidation

Melanson, Edward L.1,2; MacLean, Paul S.1,2; Hill, James O.2,3

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Exercise and Sport Sciences Reviews: April 2009 - Volume 37 - Issue 2 - p 93-101
doi: 10.1097/JES.0b013e31819c2f0b
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Abstract

INTRODUCTION

Impairments in fat metabolism seem to be important factors in the development of obesity and diabetes. For example, reduced rates of fat oxidation (i.e., increased resting respiratory quotient (RQ)) have been shown to predict weight gain (30), and obese and insulin-resistant individuals have a decreased capacity to oxidize fatty acid (FA) (11). Moreover, FA oxidation in isolated skeletal muscle preparations from obese individuals is lower than that in muscle from lean individuals (13). Exercise acutely increases fat oxidation, and endurance training increases the capacity to oxidize fat (10), suggesting that regular exercise could induce loss of fat mass by increasing fat oxidation. Although endurance training increases the capacity of skeletal muscle to oxidize all substrates, including FA, carbohydrate is still the predominant energy source in exercising skeletal muscle. It has been suggested that the major effect of exercise on fat metabolism may occur after exercise, although this has never clearly been demonstrated. Thus, how exercise leads to a reduction in fat mass is still not completely understood. To reduce fat mass, a state of negative fat balance must be achieved. To achieve negative fat balance, one must alter intake or expenditure such that fat oxidation exceeds fat intake. Because fat oxidation is increased during exercise, and because endurance exercise training increases the capacity to oxidize fat, by extension, it is assumed that more fat is oxidized on a day that exercise is performed, and that trained individuals will oxidize more fat during 24 h compared with sedentary individuals. We have conducted several studies in humans of varying adiposity and training status to determine the effects of exercise on 24-h fat oxidation. Our studies have been conducted using whole-room indirect calorimetry. An important element of these studies is that the 24-h measurement period encompasses both exercise and meals, thus reflecting a typical 24-h pattern. This differs from the majority of the studies in the literature on the effects of exercise and exercise training on fat metabolism that have been conducted in laboratory conditions in the fasted state. We have also measured 24-h substrate oxidation under sedentary and exercise conditions to provide a more complete picture of the responses to exercise. Before commencing these studies, our hypothesis was that fat oxidation would be elevated on days when exercise was performed and that fat oxidation would be higher in endurance-trained individuals. To our surprise, we have found that exercise has little, if any, effect on 24-h fat oxidation. Thus, there is an apparent paradox between the effects of acute and chronic exercise on fat metabolism in skeletal muscle versus the effects on 24-h fat oxidation. The purpose of this article is to summarize the effects of exercise on fat metabolism and discuss the effects of exercise on 24-h fat oxidation.

EFFECTS OF EXERCISE ON FAT METABOLISM

Before considering the effects of exercise on 24-h fat oxidation and fat balance, it is worthy to briefly review the effects of acute exercise and chronic exercise training on fat metabolism. An extensive review of this topic is beyond the scope of this manuscript, but the reader is referred to excellent recent reviews by Kiens (12), Jeukendrup (10), and Achten (1).

Effects of Exercise on Fat Metabolism in Skeletal Muscle

  1. The sources of lipid available to skeletal muscle duringexercise are circulating very-low-density lipoprotein-triglycerides (VLDL-TG), intramuscular triglyceride (IMTG), and circulating albumin-bound long-chain FA (LCFA) derived from lipolysis of subcutaneous and visceral adipocytes. The effects of exercise on oxidization of the different sources of fat have been extensively studied, and the evidence clearly indicates that the primary source of FA in exercising skeletal muscle is LCFA, especially during prolonged or low-intensity (LI) exercise. The contribution of VLDL-TG to total fat oxidation during exercise is low (∼5% of total energy expenditure). VLDL-TG hydrolysis is regulated by the activity of lipoprotein lipase (LPL), which is unchanged during moderate exercise, but is markedly elevated after exhaustive or glycogen-depleting exercise. Some evidence suggests that VLDL-TG oxidation may increase after exercise or with an increased intake of dietary fat, but the overall contribution to whole-body fat oxidation is still substantially less than that of LCFA. The available evidence also suggests that IMTG may provide up to 10% of total energy expenditure during prolonged exercise (≥1 h), although the contribution may be slightly higher in female subjects, and that oxidation of IMTG may be increased in the postexercise period, if subjects remain fasted.
  2. The uptake of free FA (FFA) by skeletal muscle during exercise is achieved via the combined processes of diffusion and protein-mediated uptake. Although initial research in this area suggested that uptake and oxidation of FFA was primarily determined by the rate of lipolysis in adipose tissue, more contemporary studies have demonstrated that several transport proteins play an important role in facilitating FFA uptake across the sarcolemma, including FA translocase (FAT/CD36), FA transport protein (FATP), and a plasma membrane-bound FA-binding protein (FABPPM). LCFA transport within the muscle is also mediated via a cytoplasmic FA-binding protein (FABPc).
  3. Transmitochondrial FA transport is dependent on a transport system involving carnitine as a carrier and two carnitine palmitoyl transferases (CPTI/CPTII). CPTI is inhibited by malonyl-CoA, the product of acetyl-CoA carboxylase and substrate of malonyl-CoA decarboxylase. Phosphorylation of these enzymes by 5′-adenosine monophosphate-activated protein kinase, sensing conditions of energy deficit (e.g., exercise), results in a decline in malonyl CoA concentrations, relieving malonyl-CoA-dependent inhibition and thereby increasing mitochondrial uptake and oxidation of FA. Although this regulation of CPTI seems to contribute to the regulation of fat oxidation during exercise, it is clear that it is not the only factor involved. Mitochondrial uptake of glycolytic products and the availability of free carnitine for the CPTI also may have a significant impact on the uptake and oxidation of FA during exercise.
  4. Endurance exercise training increases the capacity of skeletal muscle fat oxidation by increasing mitochondrial density, the activity of enzymes involved in β-oxidation, and oxygen delivery to the muscle. Recent evidence also suggests that endurance training increases the gene expression and protein content of several FA transporters, which may aid in the uptake and delivery of FA to the mitochondria.
  5. Whether training alters the source of FA oxidized during exercise is not clear. Training increases LPL activity, and LPL activity is higher in trained individuals, which suggest that VLDL-TG oxidation may be increased by training. These adaptations may be augmented by the increased capillarization within the trained muscle, which increases the surface of the endothelium, thereby not only increasing the binding sites for LPL but also decreasing perfusion distance from the endothelium to the plasma membrane. IMTG content has been shown to increase with training, but it seems that this adaptation requires that adequate amounts of fat are included in the diet. Whether IMTG use increases with training is controversial.
  6. At the same absolute workload, whole-body lipolytic rates are similar in sedentary and endurance-trained individuals and are not affected by endurance training; thus, delivery of LCFA to skeletal muscle is the same. However, during exercise performed at the same relative intensity, lipolytic rate is greater in trained individuals and is increased with training. Although the mechanism is not known, the increase may be related to the greater absolute work being performed.

Exercise and Fat Oxidation

It is well established that whole body fat oxidation increases with exercise intensity up to approximately 55%-65% of V˙O2max but decreases at higher exercise intensity (1,22). Why fat oxidation decreases at high exercise intensities is not completely understood, but evidence suggests a decrease in FFA availability because of a decrease in blood flow to adipose tissue, a limited capacity per unit time to generate adenosine triphosphate from oxidation of plasma FFA, or a decrease in the activity of CPT1 (1). The exercise intensity at which maximal fat oxidation rates occur varies according to training status, sex, and mode of exercise. Maximal fat oxidation rates (∼05.-0.6 vs ∼0.4-0.5 g.min−1 min−1) and the intensities at which this occurs (59%-64% vs 47%-52% of maximal oxygen consumption (V˙O2max)) are higher in highly trained versus moderately trained individuals. Maximal fat oxidation rates seem to be higher in women compared with men; fat oxidation rates are higher at a given exercise intensity in female subjects and maximal fat oxidation rates occur at a higher exercise intensity. Although the mechanism is not clear, the ability of female subjects to use more fat during exercise may be due to differences in levels of circulating hormones and catecholamines, a more oxidative muscle fiber type distribution, an increased sensitivity to catecholamine-stimulated lipolysis, or increased activity of hormone-sensitive lipase (1). Maximal fat oxidation rates also seem to be higher during walking and running compared with cycling, which may reflect recruitment of a smaller muscle mass and a lower catecholamine response during cycling (1).

It is also well established that endurance training increases fat oxidation during submaximal exercise (1). These adaptations have commonly been observed in response to moderate-intensity (60%-75% of V˙O2max) exercise training programs lasting 6-12 wk. A few studies have shown that daily moderate-intensity training sessions (2 h·d−1) induce an increase in fat oxidation during exercise within 7-10 days. In addition, sprint interval training at very high power outputs (150%-300%) produce similar increases in oxidative capacity as does moderate-intensity longer duration training programs, and high-intensity (HI) interval training (90%) has been shown to elicit similar increases in as little as 2 wk (5).

The effect of resistance training of fat oxidation has not been as well studied as the effects of endurance training. Most studies in this area have measured the acute effects of resistance exercise on fat oxidation in the period after exercise. These studies have reported that RQ is significantly decreased immediately after or the day after a bout of resistance exercise relative to a nonexercise control day, findings that have been interpreted to suggest that fat oxidation is enhanced in the postexercise period (1). The effects of regular resistance training on fat oxidation are unclear, with some studies reporting an increase and others showing no change (1).

EFFECTS OF EXERCISE ON 24-H FAT OXIDATION

Examining the independent effects of exercise on 24-h substrate oxidation requires proper attention to the state of energy balance and macronutrient intake. If subjects are in positive energy balance (intake > expenditure), carbohydrate oxidation increases, and fat oxidation decreases (9). Conversely, if subjects are in negative energy balance (intake < expenditure) carbohydrate oxidation decreases, and fat oxidation increases. Macronutrient intake will also affect fat oxidation. Carbohydrate overfeeding yields an immediate and profound shift in fuel use, suppressing the oxidation of fat and increasing carbohydrate oxidation and energy expenditure. In contrast, fat is not effective in promoting its oxidation during overfeeding and also does not stimulate an increase in energy expenditure (8).

Prestudy diet and exercise can also affect fat oxidation. For example, increasing the intake of dietary fat from 20% to 50% causes a substantial reduction in 24-h RQ within 24 h (24). With regard to exercise, Schrauwen et al. (25) have demonstrated that performance of glycogen-depleting exercise performed the day before measurement in a room calorimeter increases fat oxidation in the subsequent 24-h period. Thus, studies aimed at determining the independent effects of exercise on 24-h fat oxidation should use procedures to control prestudy exercise as well as energy and macronutrient intake.

Early Studies

Relatively few studies have considered the effects of exercise on 24-h fat oxidation. It is likely that the small size of some of the earliest room calorimeters prohibited the use of exercise equipment. In addition, early calorimeters had a slower response time and could not accurately measure energy expenditure and substrate oxidation during short intervals. One of the earliest studies using indirect room calorimetry to study the effects of exercise on 24-h substrate oxidation was performed in the calorimeter located in Lausanne, Switzerland (2). Ten male subjects were studied for 42 h, and the primary aim was to determine the effects of exercise on energy expenditure. The subjects entered the room on day 1 and performed 3 h of exercise at 50% of V˙O2max on day 2. RQ was lower in the period after exercise compared with the same period on the nonexercise day, leading the authors to conclude that fat oxidation was increased by exercise. However, it is likely that subjects were in a state of negative energy balance on the exercise day, which would have artificially elevated fat oxidation. Indeed, it was estimated that the EE during the 3-h exercise trial was 2100 kcal, but intake was only approximately 800 kcal higher on the exercise day. There was also no indication of prestudy dietary or exercise control.

In a subsequent study performed at the University of Alabama, Treuth et al. (28) compared the effects of low- intensity (LI) versus HI exercise on 24-h energy expenditure and substrate oxidation. Eight women were studied for 24-h periods that involved either LI exercise (60 min of stationary cycling at 50% of V˙O2max) or HI exercise with an identical work output (interval cycling with 2-min exercise at a workload corresponding to 100% V˙O2max with 2-min recovery). Prestudy dietary control was achieved using food records to record intake for 3 d and instructing subjects to consume the same diet before each testing day. Energy intake during the calorimeter stay was 1.3 times the resting metabolic rate (RMR), with a macronutrient composition of 65% carbohydrate, 20% fat, and 15% protein. As a group, subjects were in energy balance during the LI trial but were in negative energy balance during the HI trial; this is because EE was significantly higher (∼2045 vs 1885 kcal) during the HI trial. Despite the fact that RQ was significantly lower during LI exercise (0.85 ± 0.03 vs 0.93 ± 0.05), there were no differences in 24 h RQ (0.86 ± 0.03 vs 0.87 ± 0.02, LI and HI, respectively) or during sleep (0.84 ± 0.03 vs 0.84 ± 0.02, LI and HI, respectively). Twenty-four-hour fat oxidation was 79 ± 21 versus 79 ± 24 g for the LI versus HI protocol. It was concluded that 24-h fat oxidation was similar because, although more lipid was used during exercise in the LI trial, more lipid was used after the HI exercise. In a similar study, Saris and Schrauwen (23) at Maastricht University studied eight obese men (body mass index (BMI) = 31±1kg·m−2) in a room calorimeter for a period of 2 d. On alternate days, subjects performed an equivalent amount of either HI or LI exercise. In the LI protocol, subjects cycled for 60 min at 38% of the maximal work capacity (maximal watts, Wmax). In the HI protocol, subjects cycled for 30 min, alternating between 80% and 50% Wmax for 2.5-min intervals. Prestudy dietary control was achieved by providing subjects fixed amounts of food that were based on a food intake diary completed before the study. During the calorimeter measurements, subjects were fed a diet that achieved individual energy balance, and the macronutrient composition of the diet was 37% fat, 48% carbohydrate, and 15% protein. Although RQ during the LI exercise was significantly lower than that during the HI exercise (0.91 ± 0.00 vs 0.93 ± 0.01), there were no differences in 24-h RQ (0.89 ± 0.01 for both conditions). Interestingly, in the postexercise period (2 h after each exercise bout), RQ tended to be higher after LI compared with HI exercise (0.90 ± 0.01 vs 0.87 ± 0.02, P = 0.06). The authors concluded that differences in substrate oxidation during HI and LI exercise are compensated for during the postexercise period, leading to similar substrate oxidation patterns over 24 h.

Studies at the University of Colorado Denver

We have performed several studies in different subject groups to examine the effects of exercise on 24-h fat oxidation. In our first study (15), 16 moderately active (3-5 h·wk−1 of exercise), nonobese adults (eight men and eight women) were studied on three occasions; a sedentary day (CON), an LI exercise day, and an HI exercise day. LI and HI exercise consisted of 400 kcal of continuous stationary cycling at 40% of 70% of V˙O2max. Strengths of our study design were as follows: 1) the inclusion of a sedentary control day, which permitted a comparison between days with and without exercise; 2) precisely matching the EE during exercise under the LI and HI conditions, rather than total work, with workloads determined before the calorimeter testing using a metabolic cart; 3) achieving energy balance for each individual subject by setting energy intake to energy expenditure measured during a preliminary stay in the calorimeter (mean energy balance across conditions was 12.5 ± 29.2 kcal for Con, 6.8 ± 42.7 kcal for LI, and 54.5 ± 24.5 kcal for HI (NS); and 4) imposing strict dietary controls before each calorimeter stay. For 3 d, before each calorimeter stay, subjects were provided a diet (30% fat, 15% protein, and 55% carbohydrate) estimated to meet free-living energy requirements (RMR × 1.5-1.8, depending on self-reported physical activity level). Although RQ during the exercise differed significantly according to exercise intensity (0.88 ± 0.01 and 0.91 ± 0.01 for LI and HI, respectively), 24-h RQ was not affected by exercise intensity (0.87 ± 0.01 and 0.87 ± 0.01 for LI, and HI, respectively) and did not significantly differ from nonexercise control condition (0.86 ± 0.01). Both 24-h EE and carbohydrate oxidation were significantly elevated on the exercise days (CON < LI = HI), but 24-h fat oxidation was not significantly different across conditions (Fig. 1). Thus, in agreement with previous studies, we found no effect of exercise intensity on 24-h fat oxidation, but our study extended the results of the previous studies by also demonstrating that fat oxidation on days with exercise is not different from a sedentary control day.

Figure 1
Figure 1:
Absolute (A) and (B) and relative (C) and (D) 24-h carbohydrate (Carb) and fat oxidation in men (M;gray bars) and women (F; open bars). Values are expressed as mean ± SE; numbers within bars are means. CON, no-exercise day; LI, low-intensity aerobic exercise day; HI, high-intensity aerobic exercise day; FFM, fat-free mass. (P > 0.05). (Reprinted from Melanson EL, Sharp TA, Seagle HM, et al. Effect of exercise intensity on 24-h energy expenditure and nutrient oxidation. J. Appl. Physiol. 2002; 92(3):1045-52. Copyright © 2002 American Physiological Society. Used with permission).

To determine the effects of obesity on 24-h fat oxidation, we have performed a similar study in 15 sedentary obese (BMI ≥ 30 kg·m−2) and 11 sedentary lean (BMI ≤ 25 kg·m−2) individuals (17). Subjects were studied on a nonexercise control day (CON), on an LI exercise day (60 min of stationary cycling at 40% of V˙O2max), and an HI exercise day (30 min at 70% of their V˙O2max). Individual energy balance was tightly controlled (∼±200 kcal) by setting energy intake to EE measured during a preliminary calorimeter stay and adjusting for the expected exercise EE, and prestudy dietary control was used as in our previous study. In obese subjects, 24-h RQ did not significantly differ under CON (0.88 ± 0.01), LI (0.90 ± 0.01), or HI (0.88 ± 0.01) conditions. In lean subjects, 24-h RQ was significantly higher during HI (0.89 ± 0.01) compared with the LI (0.86 ± 0.01) and CON (0.86 ± 0.02). Twenty-four-hour fat oxidation did not differ across conditions and was 90 ± 13, 79 ± 16, 86 ± 15 g·d−1 in obese individuals (CON, LI, and HI, respectively) and 92 ± 13, 92 ± 16, 73 ± 15 g·d−1 in lean individuals (Fig. 2). Thus, consistent with our previous studies, there was no evidence that 24 h fat oxidation was increased with exercise. Eleven of the obese individuals were restudied after achieving a diet-induced weight loss (9.8% ± 3.0%), and 24-h RQ was still not significantly different across conditions (0.87 ± 0.02, 0.87 ± 0.02, and 0.84 ± 0.01 for CON, LI, and HI, respectively) (19), suggesting that weight loss does not increase daily fat oxidation on days when exercise is performed.

Figure 2
Figure 2:
Twenty-four-hour substrate oxidation in obese (top) and lean (bottom) subjects. CON, no-exercise day; LI, low-intensity aerobic exercise day; HI, high-intensity aerobic exercise day; Carbo, carbohydrate.

We also compared the effects of exercise on 24-h substrate oxidation in older (60-75 yr, n = 7) and younger (20-30 yr, n = 7) men (18). The men were studied on a day without exercise (CON) and a day with exercise (EX, 300 kcal of stationary cycling performed at 60% of V˙O2max). We used the same methods to achieve energy balance as in our previous studies and imposed the same level of prestudy dietary control. In agreement with our other studies, exercise did not affect 24-h RQ in either the older (0.85 ± 0.05 vs 0.87 ± 0.03 on the CON and EX days, respectively) or younger men (0.90 ± 0.03 vs 0.92 ± 0.03 on the CON and EX days, respectively). Fat oxidation (Fig. 3) also was not different on the CON and EX days in either the older (106 ± 21 vs 98 ± 14 g·d−1, respectively) or younger men (66 ± 8 vs 57 ± 9 g·d−1).

Figure 3
Figure 3:
Twenty-four-hour substrate oxidation in older (OM) and younger men (YM) on the no-exercise day (CON) and exercise (Ex) days. Error bars are SE. *YM versus OM,P < 0.05. †CON versus Ex, P < 0.05. (Reprinted from Melanson EL, Donahoo WT, Grunwald GK, and Schwartz R. Changes in 24-h substrate oxidation in older and younger men in response to exercise. J. Appl. Physiol. 2007; 103(5):1576-82. Copyright © 2002 American Physiological Society. Used with permission).

Effects of Endurance Training

To our knowledge, no studies have considered the effects of endurance training on 24-h fat oxidation. Because training increases fat oxidation during exercise, it is widely assumed that 24-h fat oxidation will be higher in trained individuals on a day when exercise is performed. To address this, we have recently studied a group of endurance-trained individuals under nonexercise and exercise conditions (20). During the exercise condition, subjects performed 60 min of stationary cycling at 55% of V˙O2max, an intensity which should be close to the maximal fat oxidation rates (1). Prestudy dietary control and energy balance were achieved as in our previous studies. We have studied nine competitive runners and triathletes (5 female subjects, V˙O2max = 46.1 ± 1.5 mL·kg−1·min−1; 4 male subjects, V˙O2max = 54.2 ± 3.6 mL·kg−1·min−1), with V˙O2max measured using stationary cycling ergometry. All subjects reported a minimum of 5 h·wk−1 of endurance training (average = 7 ± 1 hr). Twenty-four-hour RQ was 0.90 ± 0.04 (mean ± SD) on the CON day and 0.92 ± 0.04 on the EX day. Twenty-four-hour carbohydrate oxidation was significantly higher in the EX compared with the CON day (459 ± 45 vs 342 ± 31 g, mean ± SE), but 24-h fat oxidation was unchanged (55 ± 9 vs 44 ± 8 g on the CON and EX day, respectively) (Fig. 4). Fat balance also was not affected by exercise (−4 ± 10 vs 26 ± 10 g). Strikingly, five of the nine athletes achieved negative fat balance on the CON day, but only two of these subjects achieved negative fat balance on the exercise day (Fig. 5). Thus, even in endurance-trained individuals, we see no evidence of an increase in 24-h fat oxidation on the day when exercise is performed. It is possible that 24-h oxidation would be higher in endurance-trained individuals compared with untrained individuals. We are currently completing studies in sedentary individuals using the same exercise protocol (55% of V˙O2max for 1 h), which will address this question.

Figure 4
Figure 4:
Twenty-four-hour substrate oxidation in endurance-trained individuals (n = 9) studied under sedentary (CON) and exercise (EX) conditions. *EX versus CON, P < 0.05. Carbo, carbohydrate.
Figure 5
Figure 5:
Individual fat balance values in endurance-trained individuals (n = 9) studied under sedentary (CON) and exercise (EX) conditions. RQ, respiratory quotient.

Resistance Exercise and Resistance Exercise Training

We have also studied the effects of acute resistance exercise on 24-h fat oxidation (14,16), using the same dietary controls as in our other studies. For obvious reasons, performing resistance exercise in a room calorimeter presents some unique challenges. Thus, resistance exercises were performed using an exercise machine that could be easily used in the calorimeter (see description in (14)). In these studies, subjects performed a 60-min circuit consisting of 4 sets of 10 exercises at 70% of exercise-specific 1 repetition maximum (RM). During sets 1-3, subjects performed 10 repetitions, but on the fourth set, subjects exercised to failure. Exercises were performed in pairs (bench press and standing mid-row, bilateral leg extension and unilateral leg curls, triceps extension and biceps curl, abdominal crunch, and military press) and were repeated every 3 min. After completion of each exercise pair, subjects were permitted to rest until the next 3-min interval. We found that 24-h fat oxidation on the day that resistance exercise was performed (68 ± 13 g·d−1) was not different to that measured on the nonexercise control day (80 ± 17g·d−1) (16) or a day where stationary cycling was performed (∼1 h at 70% V˙O2max) (75 ± 12 vs 95 ± 18 g·d−1, respectively) (14).

We are aware of only one study that determined the effects of strength training on 24-h fat oxidation (27). Thirteen older women (67 ± 1 yr) were studied before and after a 16-wk resistance exercise training program. Diet for the 3 d before and during the calorimeter stays was identical before and after training. Body weight, body fat, and fat free mass did not change. Twenty-four-hour EE was unchanged, but 24-h RQ (0.90 ± 0.01 vs 0.82 ± 0.01) and 24-h carbohydrate oxidation (180 ± 4 vs 113 ± 10 g·d−1) significantly decreased, and 24-h fat oxidation increased (42 ± 6 vs 81 ± 7 g·d−1). Energy balance was not reported, but it seems that subjects were in positive energy balance during both stays, which would have the tendency of increasing carbohydrate oxidation. Whether the older age of these individuals contributed to this result is not clear. However, it is worth noting that the baseline 24-h fat oxidation in this group (42 ± 6 g·d−1) is much lower than we have observed in our studies of either younger men and women (∼60-90 g·d−1) or older men (∼90-100 g·d−1).

WHY DOESN'T 24-H FAT OXIDATION INCREASE WHEN EXERCISE IS PERFORMED?

If acute exercise and chronic exercise training induce changes in skeletal muscle that favor an increase in fat oxidation, why doesn't 24-h fat oxidation increase when exercise is performed? The most likely factor is the effect of carbohydrate intake on lipolysis and fat oxidation. It is important to note that most studies of the effects of exercise on fat metabolism have been performed with subjects in the fasted state. In room calorimeter studies, the length of the measurement period (≥24 h) necessitates that subjects be fed meals. Thus, in the room calorimeter studies performed to date, exercise has been performed in the postabsorptive or postprandial state. We would argue that this approach represents a more typical daily pattern for most individuals. However, ingesting as little as 60 g (240 kcal) of carbohydrate during the hour before exercise can reduce lipolysis and fat oxidation during subsequent exercise (3), and the increase in fat oxidation during exercise can be blunted for up to 6 hours after consumption of a meal (21). This is largely caused by an insulin-induced suppression of lipolysis (7,21), but a decrease in CPT activity also has been suggested (4). Consequently, the exercising muscle will increase the oxidation of glycogen to compensate for the decrease in fat oxidation (7). Moreover, the endurance training-induced increase in fat oxidation is prevented when a carbohydrate-rich diet is consumed (6).

Another possible explanation for the lack of evidence supporting an effect of exercise on 24-h oxidation is that the magnitude of increase in fat oxidation (e.g., <10 g·d−1) is below the sensitivity of most room calorimeters. The trends we see would tend to argue against this possibility. In our studies, we have consistently observed a slightly higher RQ and lower fat oxidation in most individual subjects on the days that exercise is performed, even in endurance-trained individuals (Fig. 4) Another factor to consider in the room calorimeter studies is that total EE is lower than would be observed in free-living individuals. However, in our studies, although 24-h EE is higher in the endurance-trained individuals compared with untrained individuals, 24-h RQ is increased on days when exercise is performed.

It is important to note that our studies have been performed with subjects in energy balance. Although studying subjects in energy balance may be perceived as artificial situation, the aims of our studies to date has been to determine the independent effects of exercise on 24 h fat oxidation, which can only be done if subjects are in energy balance. This may not be the ideal metabolic context in which the effect of exercise would emerge. Exercise may prime peripheral tissues to respond differently to a metabolic challenge, like overfeeding or calorie restriction. For example, Smith and colleagues (26) have eloquently demonstrated that adaptation to an increase in dietary fat is more complete when high levels of physical activity are performed. Thus, the impact of exercise may become apparent in the free-living environment, under certain metabolic challenges or feeding patterns that we have yet to recapitulate in the laboratory.

It could be argued that, although exercise does not impact 24-h RQ or fat oxidation, exercise may improve the ability to modulate fuel use. This could theoretically lead to a compensation in fat oxidation in the postexercise period, perhaps during sleep. For example, Saris and Schrauwen (23) reported that differences in substrate oxidation during LI and HI exercise were compensated in the postexercise period. In that study, RQ was lower during exercise on the LI day compared with the HI day but tended (P = 0.06) to be higher (on average) during the 2 h immediately after exercise. However, sleeping RQ was not affected, and fat balance did not differ between conditions. Importantly, subjects were in positive fat balance under both conditions. Thus, in the context of 24-h fat balance, small differences in fat oxidation in the postexercise period seem to have no effect on fat balance. We routinely compare 24-h RQ profiles within subjects and have not found evidence that a meaningful compensation occurs, even in endurance-trained individuals (Fig. 6). Finally, in most of the subjects we have studied, 24-h RQ is slightly higher, and fat oxidation is slightly lower on the exercise day compared with the nonexercise day, although, on a group level, these differences are not significant. This suggests that the effects of diet in modulating 24-h substrate oxidation are much more potent than the effects of exercise, assuming energy balance.

Figure 6
Figure 6:
Representative 24-h RQ profile in an endurance-trained individual under sedentary (CON) and exercise (EX) conditions. Thebolded arrow indicates the exercise period.

The finding that exercise does not increase 24-h fat oxidation when individuals are studied in energy balance provokes two questions. First, how do endurance-trained individuals maintain a low fat mass? Fat mass will not decrease unless fat oxidation exceeds fat intake. Thus, it is possible that endurance-trained individuals maintain a low-fat mass because their habitual fat intake is lower than their total fat oxidation. It is also possible that individuals participating in regular endurance training are in a state of negative energy balance on most of their training days. It seems reasonable that an individual expending high levels of energy (e.g., 800 or more kcal·d−1) may not completely replace the calories expended during training. Even if this difference were small (e.g., <100 kcal), it would create a state of negative energy balance and increase 24-h fat oxidation, and the repetitive effect over sequential days could have a substantial effect on fat mass. However, our ability to detect such small differences, especially in free-living individuals, is limited. Another factor to consider is the effect of consecutive days of HI training. As mentioned previously, glycogen-depleting exercise increases fat oxidation in the subsequent 24-h period (25). Thus, if glycogen stores are not completely restored before the subsequent exercise training session, fat oxidation may be increased. Additionally, it should be mentioned that endurance-trained individuals are likely to perform some training sessions for longer durations than we have studied so far. Because LCFA becomes a much more important fuel source with increasing exercise duration, and also because this type of training is likely to deplete muscle glycogen stores, it is possible that 24-fat oxidation is increased in response to long-duration exercise bouts (e.g., >1 h). Finally, we have only studied responses to cycling exercise, which as previously discussed, may have lower maximal fat oxidation rates compared with running and walking.

The second question is how can exercise be used to increase fat oxidation in those individuals who desire to use exercise to lose fat mass? For example, because the increases in fat oxidation are most pronounced in the fasted state, would exercising in the fasting state promote an increase in 24-h fat oxidation? This is an intriguing possibility, but it cannot be ruled out that there would be compensatory decrease in fat oxidation in the postexercise period once a meal was consumed. Moreover, because fat oxidation is suppressed for up to 6 h after consumption of carbohydrate, it is not likely that most individuals would find this to be an appealing option. Another possibility is that 24-h fat oxidation may be increased by altering the type of fat consumed. For example, Votruba et al. (29) have demonstrated that previous exercise increases the oxidation of dietary oleate but not dietary palmitate, suggesting that exercise increases monounsaturated fat oxidation more than saturated fat oxidation. Thus, recommendations for exercise for the purposes of reducing fat mass may need to be specific for dietary fat composition.

CONCLUSIONS

Implicit in our review is the notion that to achieve a decrease in fat mass, a state of negative fat balance must be achieved. A model of how negative fat balance may be achieved is presented in Figure 7. To achieve negative fat balance, one must alter intake or expenditure such that fat oxidation exceeds fat intake. Energy and macronutrient intake are powerful modulators of fat balance. If energy intake is less than expenditure, fat oxidation will increase, independent of macronutrient intake, inducing a state of negative fat balance. Negative fat balance can also be achieved even when in energy balance, provided that fat intake is less than oxidation. Ironically, high-fat diets will increase fat oxidation, but in this instance, negative fat balance would only be achieved if the oxidation rate exceeds the intake. In our model, the effects of high levels of physical activity and exercise remain unclear. It is widely assumed by both the scientific community and the general public that high levels of physical activity and exercise would induce an increase in fat oxidation and therefore negative energy balance. Our studies would suggest that energy and macronutrient intake is a more important modulator of daily fat balance and, therefore, that exercise recommendations made for the sake of regulating fat mass cannot be made without also considering energy and macronutrient intake. Unfortunately, at this time, we cannot make recommendations of how energy and macronutrient intake can be modulated to induce negative fat balance. We can state with a degree of confidence that if energy intake is maintained below expenditure, then exercise will induce a state of negative fat balance. However, whether manipulations of macronutrient intake will be as effective remains to be demonstrated. Finally, our studies have only considered acute exercise (i.e., exercise performed on a single day in the calorimeter). The effects of consecutive days of exercise on fat balance remains to be studied.

Figure 7
Figure 7:
Proposed model to explain the effects of dietary intake and fat oxidation on fat balance. Negative fat balance is achieved when energy expenditure exceeds energy intake or when fat oxidation exceeds fat intake. Although the effects of dietary manipulation on fat balance is well understood, the effects of exercise and physical activity (PA) on fat balance are not as well characterized.

The evidence that level of exercise affects body weight is strong, but our incomplete understanding of why could limit our ability to develop optimum strategies for using exercise to address the current epidemic of obesity. On one hand, we can just be content with a recommendation to the population to increase exercise. However, a more complete understanding of the impact of exercise on energy balance may help develop more specific and better ways to optimize the impact of exercise on body weight. This will require studies that go beyond assessing the amount and type of energy intake in response to exercise and the impact of exercise on macronutrient oxidation rates. This also will require studies where exercise and energy intake are combined in ways that reflect how exercise is done in daily life. Although these studies are difficult, we believe that we must understand the interaction of exercise and food intake on energy and macronutrient balance that may provide the most useful information.

Although we do not discount the numerous benefits of regular endurance exercise on various aspects of health (e.g., improving lipid profiles and insulin sensitivity), we do think that it is time to reconsider how we promote exercise as a means of inducing fat loss. Indeed, the plethora of studies that demonstrate that exercise produces less than an expected weight loss seems consistent with our conclusion that, in nonfasted individuals, exercise has little effect on daily fat oxidation. We offer that in individuals performing moderate amounts of exercise (e.g., <1 h·d−1); the primary benefit of endurance exercise on body weight may be in preventing additional weight gain or in preventing weight regain after weight loss, but understanding exactly how to prescribe exercise to achieve fat mass loss requires further investigation. Finally, we suggest that it is time to put the myth that LI exercise promotes a greater "fat burn" to rest. Clearly, exercise intensity does not have an effect on daily fat balance, if intake is unchanged. We must educate the public that participation in moderate doses of exercise will not burn more fat unless changes are made to energy or fat intake.

These studies could not have been performed without the assistance of the Nursing, Clinical Lab, and Dietary Staff of the University of Colorado Denver Clinical Translational Research Center (CTRC). We are grateful for their support.

The study described within has been supported by the following grants: Institutional National Research Service Award (National Institutes of Health [NIH] AG00279) and Mentored Scientist Award (NIH K01DK061348) (Dr. Melanson), NIH R01 DK42549, HL 049331 (Dr.Hill), The University of Colorado GCRC (NIH M01 RR00051).

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Keywords:

exercise training; skeletal muscle; substrate metabolism; oxidative capacity; indirect room calorimetry

©2009 The American College of Sports Medicine