It Can't Be a Coincidence: A Comparison of Cases of Autoimmune Hepatitis After Vaccination Against COVID-19 : ACG Case Reports Journal

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It Can't Be a Coincidence: A Comparison of Cases of Autoimmune Hepatitis After Vaccination Against COVID-19

Gips, Julia R. MD1; Woreta, Tinsay A. MD, MPH1

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ACG Case Reports Journal 10(1):p e00965, January 2023. | DOI: 10.14309/crj.0000000000000965
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The novel coronavirus disease 2019 (COVID-19) has had a devastating impact on the world, leading to the rapid development of vaccinations to prevent serious disease. Since the wide distribution of these vaccinations, there have been multiple case studies associating the COVID-19 vaccine with autoimmune hepatitis (AIH) (Table 1).

Table 1. - Comparison of case reports
Case report Patient history, laboratory values on presentation, and course
Authors Sex, age Autoimmune disease history Vaccine received AST (U/L) ALT (U/L) Auto-antibody positive? IgG level (mg/dL) Histology Outcomes
Vuille-Lesard et al 7 F, 76 Hashimoto Moderna 811 579 ANA 1:1,280, ASMA 1:1,280, anti-actin 84U, ANCA >1:1,280 3,940 Active hepatitis with interface hepatitis, plasma cells, feathery degeneration and pseudorosettes Successfully treated with 40 mg prednisolone
Ghielmetti et al 4 M, 63 None Moderna 1,127 1,038 ANA 1:640, anti-gastric parietal cells antibody 1:320 1,996 Interface hepatitis, lobular and centrilobular inflammation with centrilobular necrosis without fibrosis or steatosis Successfully treated with 40 mg prednisone
Avci and Abasiyanik 8 F, 61 Hashimoto Pfizer 913 455 ANA 1/100 (<1/100 negative), ASMA 1/100 (<1/100 negative) 4,260 Lymphocyte infiltration, severe portal and periportal inflammation, interface hepatitis, and mild fibrosis Successfully treated with 20 mg prednisone
Bril et al 9 F, 35 None, patient postpartum Pfizer 754 2,001 ANA 1:1,280, dsDNA 1:80 1,081 Panlobular hepatitis and lymphoplasmacytic infiltrate with rosette formation and scattered hepatocyte necrosis Successfully treated with 20 mg prednisone
Lodato et al 10 F, 43 None Pfizer 51 52 Negative WNL Portal inflammatory infiltrate and interface hepatitis with biliary injury and mild ductular proliferation Successfully treated with 1 mg/kg/d methylprednisolone
McShane et al 11 F, 71 None Moderna 1,469 1,067 ASMA 1:2,560 2,177 Inflammatory cell infiltrate including eosinophils, interface hepatitis, and periportal/portal central bridging necrosis Successfully treated with prednisolone
Rocco et al 12 F, 80 Hashimoto Pfizer 1,401 1,186 ANA 1:160 3,500 Interface hepatitis with a moderate degree of lymphoplasmacytic infiltrate and confluent foci of lobular necrosis Successfully treated with 1 mg/kg/d prednisone
Londono et al 13 F, 41 None Moderna 993 1,312 ANA 1:80, ASMA 1:40, and anti-SLA-positive 2,080 lymphoplasmacytic infiltration, severe interface hepatitis and lobular inflammation with necroinflammatory foci, apoptotic bodies, and hepatocyte ballooning. Successfully treated with 1 mg/kg/d prednisone
Clayton-Chubb et al 14 M, 36 None Oxford-AstraZeneca 633 1,744 ANA 1:160 1,280 Interface hepatitis with a lymphocytic inflammatory infiltrate without fibrosis Successfully treated with prednisolone 60 mg
Rela, M. et al 15 F, 38 Hypothyroid (not specified if Hashimoto) Covishield 1,101 1,025 ANA 1:80 1,650 Multiacinar hepatic necrosis and diffuse portal and periportal neocholangiolar proliferation Successfully treated with 30 mg prednisolone
M, 62 None Covishield 1,361 1,094 Negative Portal central bridging necrosis and lymphoplasmacytic inflammation with mild portal fibrosis Treatment attempted with 30 mg prednisolone and plasma exchange; however, his liver function did not recover, and he died 3 weeks into admission
Tan et al 16 F, 56 None, patient postpartum Moderna 1,124 1,701 ANA and ASMA-positive 3,260 Portal inflammation with interface hepatitis, lobular inflammation with plasma cell aggregates, rosette formation, and apoptotic hepatocytes Successfully treated with budesonide
Tun et al 17 M, 47 None Moderna 1,048 ANA-positive 2,510 Widespread bridging necrosis, marked interface hepatitis, lymphoplasmacytic infiltration including eosinophils, ballooned hepatocytes, multinucleated giant cells, and emperipolesis Successfully treated with prednisolone 40 mg
Camacho-Dominguez et al 18 M, 79 None AZD1222 2,003 1,994 ANA 1:80, ASMA 70 U 2,058 Focal cholestasis and lobulation of the parenchyma, marked ductular proliferation, lymphocytic infiltrate in the portal spaces with the presence of eosinophils Successfully treated with hydrocortisone and prednisone
ALT, alanine transaminase; ANA, anti-nuclear antibody; ANCA, anti-neutrophil cytoplasmic antibody; ASMA, anti-smooth muscle antibody; AST, aspartate aminotransferase; IgG, immunoglobulin G; SLA, soluble liver antigen; WNL, within normal limits.

The pathogenesis of AIH is believed to be an environmental trigger leading to T-cell-mediated destruction of hepatocytes, chronic inflammation, and fibrosis of the liver.1 Diagnosis of AIH requires elevated aspartate aminotransferase (AST), alanine transaminase (ALT), and immunoglobulin G (IgG); the presence of associated antibodies (type 1 AIH: anti-nuclear antibody [ANA] and anti-smooth muscle antibody [ASMA] vs type 2 AIH: anti-liver kidney microsome type 1), and pathology demonstrating lymphoplasmacytic interface hepatitis.2 While AIH does have known markers of disease, it remains a diagnosis of exclusion.

The mechanism behind COVID-19 vaccination-induced autoimmune disease is believed to be related to molecular mimicry. Studies have shown that there is significant peptide sharing between an antigen of COVID-19, severe acute respiratory syndrome coronavirus 2 spike glycoprotein, and mammalian proteins, which may lead to T-cell cross-reactivity.3 Other hypotheses suggest that the vaccine's activation of the interferon pathway may lead to autoimmune conditions.4 Furthermore, the COVID-19 virus itself also has an affinity for the liver as both hepatic and bile duct cells express angiotensin-converting enzyme 2, an enzyme which allows the virus to invade cells.5,6

Although rare, there have been case reports describing AIH after COVID-19 vaccination, which we compared in Table 1. Multiple vaccine formulations have led to AIH cases, including Pfizer, Moderna, AstraZeneca, and Covishield, to name a few. Among the patients described in the literature, 64% were women; 62% were older than 50 years; and all had biopsy-proven AIH with histology showing interface lymphoplasmacytic hepatitis. Two patients had preexisting autoimmune disease, both involving the thyroid, and 2 patients were postpartum at the time of diagnosis. All patients had elevated AST and ALT (with both values >500 in 93% of cases) and elevated total bilirubin. 75% had elevated IgG levels, and 86% were positive for ANA, ASMA, or both. One patient in the cohort was unable to recover liver function, but all others responded well to steroid treatment and were able to taper their immunosuppression completely over the subsequent months or to the minimum dose required to prevent reactivation of disease.

In this study, we present 1 such case of AIH after vaccination against COVID-19. A 72-year-old man with a history of well-controlled pulmonary sarcoidosis presented with scleral icterus 2 weeks after receiving his first dose of COVID-19 Pfizer vaccination. Blood work showed aminotransferase values in the 1000s. He had never been told before this that he had liver problems, which was confirmed with medical record review. He endorsed significant fatigue, stomach upset, nausea, constipation, and anorexia. He lost 11 pounds over a 2-month period.

His home medications included vitamins D3, B12, and Echinacea supplements. He was asked to stop all supplements when the jaundice began, with no improvement in transaminase values. Abdominal CT showed diminished attenuation of the liver suggesting steatosis. He was referred to our tertiary care hepatology clinic.

On physical examination, he appeared fatigued with icteric sclera. Abdominal examination was grossly unremarkable. Laboratory data on initial presentation showed white blood cell count (WBC) 6.9 K/cu mm; hemoglobin 13.8 g/dL; mean corpuscular volume 96 fL; platelets 267 K/cu mm; creatinine 1.4 mg/dL; total bilirubin 7.2 mg/dL; alkaline phosphatase 215 U/L; AST 1,177 U/L; and ALT 1,221 U/L. Follow-up laboratory test results were notable for peak values as follows: WBC 22.4 K/cu mm; AST 1,234 U/L; ALT 1,221 U/L; total bilirubin 18.6 mg/dL (direct 14.8); iron 256 ug/dL; ferritin 3,066 ng/mL; and international normalized ratio (INR) 1.1. Viral hepatitis panel showed negative hepatitis A IgM, negative hepatitis B surface antigen, negative hepatitis B core IgM, and negative hepatitis C antibody. ASMA was weakly positive (1:22), and ANA direct was positive. Anti-mitochondrial antibody and anti-liver kidney microsome type 1 antibody were negative; alpha-1 antitrypsin was mildly elevated at 208 mg/dL; ceruloplasmin was within normal range; and hemochromatosis gene testing was negative. Serum IgG was elevated at 3,480 mg/dL. Liver biopsy showed fulminant hepatitis with severe panlobular plasma cell-rich inflammation, extensive hepatocellular swelling, and frequent acidophil bodies. Reticulin stain showed evidence of chronic injury and fibrosis, but it was not clear whether this was true fibrosis or a result of his acute presentation. Ki-67 stain was consistent with regenerative response.

The patient was initiated on prednisone 60 mg daily. Laboratory test results after treatment initiation showed significant decrease in liver enzymes. The prednisone dose was tapered by 10 mg weekly until he reached a dose of 30 mg. His liver enzymes did not fully normalize and plateaued with an ALT of 118 IU/L and AST of 56 IU/mL. He was thus started on mycophenolate mofetil 1 g BID with complete normalization of liver enzymes over the subsequent month. He was then weaned to 500 mg BID of mycophenolate for 30 days before stopping his immunosuppressive regimen completely. He was advised to avoid further vaccination against COVID-19.

In this report, we presented the case of a 72-year-old man with onset of severe, biopsy-proven AIH just 13 days after receiving his first dose of the Pfizer COVID-19 vaccine. There is now a well-documented cohort of patients presenting with autoimmune response to the COVID-19 vaccine. What we can conclude from the literature thus far is that this response is more common in women older than 50 years without preexisting autoimmune disease, presents as type 1 AIH, and responds well to steroid treatment. At this point, AIH has been observed with most vaccination formulations. Of course, this should not deter anyone from receiving the vaccine against COVID-19, but instead give providers something to add to the differential when a patient presents with a new, unexplained hepatitis.


Author contributions: J. Gips contributed to the concept and writing of the article. T. Woreta contributed to the concept and editing of the report and is the article guarantor.

Acknowledgments: We thank Robert A. Anders for assistance with pathology.

Financial disclosure: None to report.

Informed consent was obtained for this case report.


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autoimmune hepatitis; COVID-19 vaccination; Adverse Response to vaccination

© 2023 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of The American College of Gastroenterology.