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Transplantation With Autologous Mesenchymal Stem Cells After Acute Myocardial Infarction Evaluated by Magnetic Resonance Imaging: An Experimental Study

Lu, Minjie MD; Zhao, Shihua MD; Liu, Qiong PhD; Jiang, Shiliang MD; Song, Peng PhD; Qian, Haiyan PhD; Zhang, Yan MD; Ling, Jian MD; Yan, Chaowu PhD; Cheng, Huaibing PhD; Ma, Ning PhD; Zhao, Hong MD; Liu, Yuqing MD

doi: 10.1097/RTI.0b013e31820446fa
Original Articles

Purpose: The purpose of this study was to track and investigate the effects of autologous bone marrow-derived mesenchymal stem cells (MSCs) transplantation after acute myocardial infarction in swine assessed by magnetic resonance imaging (MRI).

Materials and Methods: Twenty-four Chinese mini-pigs (27±3 kg) were divided into 4 groups, including control groups (groups 1 and 3) and MSCs transplantation groups (group 2, super paramagnetic iron oxide labeled and group 4, 4′,6-diamidino-2-phenylindole labeled). Super paramagnetic iron oxide-labeled and 4′,6-diamidino-2-phenylindole-labeled MSCs (3.0×106 cells/mL) with a volume of 10 mL were injected into the left anterior descending artery by a catheter at 1 week after acute myocardial infarction, respectively. Cell distribution, cardiac functions, and scar tissue were quantitatively assessed by MRI.

Results: The reduction of the T2* value in the myocardium, spleen, and liver in group 2 was significantly greater than that in group 1. MRI showed that function and scar size at baseline and 3 days after cell infusion were not significantly different between groups 1 and 2. Six weeks later left ventricular ejection fraction (P<0.0001), end-systolic volume (P<0.05), the number of dyskinetic segments (P<0.0001), left ventricular weight index (P<0.0001), and the infarcted size (P<0.0001) in group 4 were all improved comparing with those in group 3.

Conclusions: The majority of MSCs entrapped by the extracardial organs were mainly in the spleen. Catheter-based delivery of autologous bone marrow-derived MSCs into infarcted myocardium is feasible and effective.

Departments of *Radiology

Cardiac Surgery

Cardiology

§Pathology and Histology, Fuwai Hospital, Peking Union Medical College, People's Republic of China

The authors declare no conflicts of interest.

Supplemental Digital Content is available for this article. Direct URL citations appear in the printed text and are provided in the HTML and PDF versions of this article on the journal's Website, www.thoracicimaging.com.

Supported by a research grant of National Natural Science Foundation of China (30471722 & 81000604) and Peking Union Medical College Research Fund for Youth (2009-xhj4♯).

Reprints and corresponding author: Shihua Zhao, MD, Department of Radiology, Fuwai Hospital, Peking Union Medical College (PUMC) and Chinese Academy of Medical Sciences (CAMS), Beijing, 100037, People's Republic of China (e-mail: coolkan@163.com).

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