Ott, Mary A. MD, MA*; Harezlak, Jaroslaw PhD†; Ofner, Susan MS†; Fortenberry, J. Dennis MD, MS*
From the *Section of Adolescent Medicine, Department of Pediatrics, Indiana University School of Medicine, Indianapolis, IN; and †Department of Biostatistics, Indiana University School of Medicine, Indianapolis, IN
Conflict of interest and source of funding: Supported by the NIH K23-HD049444–01, R01 HD 044387. No authors report a conflict of interest.
Correspondence: Mary A. Ott, MD, MA, Section of Adolescent Medicine, Indiana University School of Medicine, 410 West 10th St, HS 1001 Indianapolis, IN 46202. E-mail: email@example.com.
Received for publication November 17, 2011, and accepted June 11, 2012.
Abstract: Among adolescents, partner changes are associated with STIs, but little is known about the timing. Using daily diaries and weekly STI tests, we describe whether infections occur before or after sex partner change during periods when a young woman changes partners once. Results showed infections occurring both before and after partner changes.
Adolescent females have the highest age-specific rates of gonorrhea and Chlamydia.1,2 Acquisition of these bacterial STI has been linked to partner change,3,4 including “serial monogamy,” or sequential nonoverlapping partners.3 Little is known about the timing of STI transmission within partner change, yet this information is important to prevention counseling.
In serial monogamy, in theory, STI transmission occurs as long as the gap between partners is shorter than the infectious period of the organism.5 In reality, many adolescents are unaware about their partner’s infidelity,6 and the detailed sexual network information needed to be assured of monogamy by both sex partners is rarely available to clinicians or researchers. Partner change is frequently a time of relationship turmoil.7,8 If a partner change is a reflection of partner distrust or suspicion that a partner is unfaithful, then STI may precede the partner change.
An analysis of where infection occurs within partner change requires frequent assessments of sexual behavior and STIs. Using a unique data set that included daily diaries of sexual behavior and weekly STI screening tests, we conducted an exploratory analysis of whether gonorrhea, chlamydia, and trichomonas infections are acquired before or after partner change among young women reporting serial monogamy, or one partner change with no overlap.
MATERIALS AND METHODS
Participants and Procedures
As part of a larger institutional review board-approved study of STI, 14- to 17-year-old female adolescents were recruited from 3 adolescent clinics serving communities with high STI prevalence, and followed for up to 8 years. Each year, participants provided two 3-month periods of daily diaries of partner-specific sexual behaviors, as well as weekly self-collected vaginal swabs for chlamydia, gonorrhea, and trichomonas amplified DNA-based testing. Positive gonorrhea tests were verified by a different DNA-based test. Tests were batched and done at the end of the 3-month period, at which point participants were informed and treated per Centers for Disease Control and Prevention guidelines.9 Details of the larger study are provided elsewhere.10
The units of analysis were any given 3-month-diary periods during the course of the study that met the following conditions. First, participants reported sex partner change during the diary period (n = 274). (In most periods, participants reported no partners or 1 partner). Second, to identify whether the infection occurred before or after partner change, we limited our analysis to intervals in which participants reported only 2 sex partners (n = 205) and did not switch back-and-forth between partners (n = 141). Third, to focus on incident STI, we eliminated diary periods in which the first swab of the period was positive for STI, leaving 111 intervals for analysis. Finally, when multiple periods from the same participant were used, periods were at least 5 months apart to minimize within-subject dependence.
Because of organism differences in transmission, chlamydia, trichomonas, and gonorrhea were analyzed separately. The date of partner change was the first report of sex with the second partner. The date of STI acquisition was the first weekly swab to test positive for that STI. STIs occurring before the date of partner change were classified as interval 1; STIs occurring on or after the date of partner change were classified as interval 2.
In the context of partner change, it is assumed that incidence of infections with the second partner (interval 2) will be more than with the first partner (interval 1). Therefore, our null hypothesis was that the proportion of infections in interval 2 (p2) would be more than that of interval 1 (p1), by some constant (c) amount:
Equation (Uncited)Image Tools
To estimate the differences in the probability of infection in interval 1 versus interval 2, we used a noninferiority type of 2-sample test with a 1-sided 95% confidence interval. A logistic regression model was used to estimate the odds ratios (ORs) of STI in interval 1 versus interval 2. We provide the 95% confidence intervals and noninferiority test results showing the smallest detectable OR for a significance level [alpha] equal to 0.05. We interpret the upper bound of the 95% CI to mean that, if a difference exists between proportion of STIs diagnosed before and after partner change, there is only a 5% chance that the difference will be larger than the upper limit of the 95% CI.
Ninety-two participants provided 111 diary periods. Most (83%) contributed 1 period, 14 contributed 2, and one each 3 and 4 periods. Participants were 94% black. The mean age of participants at the time the data were collected was 17.8 years.
Figure 1 shows results for timing of sex and infection for 6 representative periods. We observed different patterns of positive tests in interval 1 versus interval 2 for chlamydia, gonorrhea, and trichomonas. For chlamydia infections, incident infections occurred both before and after partner change, with 11 (9.9%) diary periods positive in interval 1 and 10 (9.0%) positive in interval 2. Similarly for trichomonas, infections were observed both in intervals 1 and 2, with 8 (7.2%) diary periods positive in interval 1 and 5 (4.5%) positive in interval 2. For gonorrhea, more infections were observed after partner change in interval 2, with 3 (2.7%) diary periods positive in interval 1 as compared with 9 (8.1%) diary periods positive in interval 2.
For chlamydia and trichomonas, we detected a moderate difference in incidence between the 2 intervals (Table 1). For these 2 organisms, we had adequate power at P < 0.05 to detect an OR of 1.8 or higher for not detecting an infection in interval 2 versus interval 1. Restated, if there is a difference in the odds of acquiring an infection in interval 2 versus interval 1, we are 95% certain it is not greater by more than 1.8. In contrast, for gonorrhea, the smallest detectable OR had an upper bound of 10. This means that, if there is a difference between interval 2 and interval 1, we are 95% certain it is not greater by more than 10. This high upper bound indicates that, for gonorrhea, interval 2 may have a much higher rate than interval 1.
This analysis extends our understanding of the association between partner change and STI acquisition. Despite the limitations of a small sample, we were able to demonstrate that many infections occur before partner change, suggesting that, in these cases, the infection was acquired from the first partner, rather than the second partner. In these cases, the STI risk was in the first relationship “going bad,” rather than the partner change itself. This is complementary to network analyses, which can miss as many as 40% to 60% of sexual partnerships.11 Our use of an urban black sample from a community with high rates of STIs may limit transferability of results to other populations; however, it is a group of importance to study because of marked STI-related health disparities.
Also of importance are organism-specific patterns of infection. This may be due in part to differences in transmissibility. Gonorrhea has been shown to be highly transmissible, and chlamydia less so.12 These patterns may also reflect differences in the development of symptoms, assuming that individuals with symptoms might have sought care and received an STI diagnosis and treatment.
These findings are consistent with research showing that young women are frequently unaware of their partners’ other partners,6 and they suggest that clinicians may want to more carefully inquire about relationships and consider enhanced screening in young women reporting relationships of lower quality, with lower levels of communication, or in turmoil.
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