Analytical Specificity and Sensitivity of the APTIMA Combo 2 and APTIMA GC Assays for Detection of Commensal Neisseria Species and Neisseria gonorrhoeae on the Gen-Probe Panther Instrument

Golparian, Daniel MSc*†; Tabrizi, Sepehr N. PhD; Unemo, Magnus PhD*†

doi: 10.1097/OLQ.0b013e3182787e45
Note

Abstract: Genetic detection of Neisseria gonorrhoeae is replacing culture for increased diagnostic sensitivity. Specificity of several nucleic acid amplification tests is suboptimal. Herein, the Gen-Probe APTIMA Combo 2 and APTIMA GC assays had 100% specificity and 100% sensitivity after confirmatory testing, when testing 298 isolates of nongonococcal Neisseria and related species and 205 gonococcal isolates.

The Gen-Probe APTIMA Combo 2 and APTIMA GC assays had 100% specificity and 100% sensitivity, when testing 298 isolates of nongonococcal Neisseria and related species and 205 gonococcal isolates.

From the *WHO Collaborating Centre for Gonorrhoea and Other STIs, National Reference Laboratory for Pathogenic Neisseria, Örebro, Sweden; †Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden; and ‡Department of Microbiology and Infectious Diseases, The Royal Women’s Hospital, Parkville, Victoria, Australia

The authors are grateful to Gen-Probe for providing the diagnostic kits used in the study.

Supported by the Örebro County Council Research Committee, the Foundation for Medical Research at Örebro University Hospital, Sweden, and Scientific Affairs, Gen-Probe Incorporated, San Diego, CA.

All authors declare that they have no conflicts of interest.

Correspondence: Magnus Unemo, PhD, WHO Collaborating Centre for Gonorrhoea and Other STIs, Department of Laboratory Medicine, Microbiology, Örebro University Hospital, SE-701 85 Örebro, Sweden. E-mail: magnus.unemo@orebroll.se.

Received for publication August 4, 2012, and accepted October 10, 2012.

© Copyright 2013 American Sexually Transmitted Diseases Association