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Antimicrobial Susceptibility Evaluation and Multiple-Locus Variable Number Tandem Repeat Analysis of Neisseria gonorrhoeae Isolates in China in 2012

Yu, Rui-xing MD; Yin, Yueping PhD; Dai, Xiu-qin; Chen, Shao-chun PhD; Han, Yan PhD; Zheng, Bing-jie PhD; Zhang, Guo-yi MD; Chen, Xiang-sheng PhD

Sexually Transmitted Diseases: April 2017 - Volume 44 - Issue 4 - p 197–201
doi: 10.1097/OLQ.0000000000000580
Original Study

Objective This study aimed to gain information on the antimicrobial susceptibility and molecular epidemiological typing of Neisseria gonorrhoeae (NG) isolates in China in 2012.

Methods A total of 244 NG isolates were consecutively recovered from patients with uncomplicated gonorrhea attending sexually transmitted disease clinics in 3 Chinese cities—Guangzhou, Nanjing, and Tianjin—in 2012. Neisseria gonorrhoeae susceptibilities to penicillin and tetracycline were examined by detecting penicillinase-producing NG (PPNG) and high-level tetracycline-resistant NG, and NG susceptibilities to ciprofloxacin, spectinomycin, ceftriaxone, and cefixime were determined using an agar dilution method. Neisseria gonorrhoeae isolates were typed by multiple-locus variable number tandem repeat analysis. We conducted a χ2 analysis to compare clusters with Bonferroni correction and Kruskal-Wallis test.

Results Neisseria gonorrhoeae isolates gathered from the 3 cities differed significantly in the prevalence of tetracycline-resistant NG (P < 0.001) and NG treated with ceftriaxone with a minimum inhibitory concentration of 0.125 mg/L or higher (P < 0.001). The analysis of the combination of the 7 variable number of tandem repeats loci for all of the 244 isolates yielded 110 multiple-locus variable number tandem repeat analysis types falling into 5 clusters. Cluster III was associated with PPNG, whereas cluster II was associated with non-PPNG (P < 0.05) and NG treated with ceftriaxone with a minimum inhibitory concentration of 0.125 mg/L or higher (P < 0.05).

Conclusions Antimicrobials that can be used with confidence to treat NG infection currently in China include ceftriaxone and spectinomycin, but not penicillin, tetracycline, ciprofloxacin, and cefixime. Moreover, some of the resulting clusters were associated with PPNG and NG with decreased ceftriaxone susceptibility.

We conducted a study of antimicrobial susceptibility evaluation and multiple-locus variable number tandem repeat analysis of 244 Neisseria gonorrhoeae isolates collected from Tianjin, Nanjing, and Guangzhou, China, in 2012.

From the *Department of Dermatology, China-Japan Friendship Hospital, Beijing; and †National Center for STD Control, Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Dermatology, Nanjing, China

Conflict of interest: None declared.

Correspondence: Yueping Yin, PhD, Reference Laboratory, National Center for STD Control, Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Dermatology, 12 Jiangwangmiao St, Nanjing 210042, China. E-mail: yinyp@ncstdlc.org.

Received for publication November 19, 2014, and accepted December 5, 2016.

© Copyright 2017 American Sexually Transmitted Diseases Association