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Prevalence and Molecular Epidemiological Typing of Penicillinase-Producing Neisseria gonorrhoeae and Their blaTEM-135 Gene Variants in Nanjing, China

Chen, Shao-Chun PhD*; Yin, Yue-Ping PhD*; Dai, Xiu-Qin MT*; Yu, Rui-Xing PhD*; Han, Yan MPH*; Sun, Hou-Hua MT*; Ohnishi, Makoto PhD; Unemo, Magnus PhD; Chen, Xiang-Sheng MD*

doi: 10.1097/OLQ.0000000000000037
Original Study

Background This study aimed to investigate the prevalence of penicillinase-producing Neisseria gonorrhoeae (PPNG) and their blaTEM-135 gene variant in 2007 and 2012 in Nanjing, China. In addition, molecular epidemiological typing of all isolates was performed to elucidate the genetic relationships of the PPNG strains.

Methods A total of 199 and 77 N. gonorrhoeae isolates were collected at the National Center for STD Control in 2007 and 2012, respectively. Nitrocefin tests were performed to identify PPNG. Mismatch amplification mutation assay was used to identify blaTEM-135. All isolates were genotyped using N. gonorrhoeae multiantigen sequence typing (NG-MAST), and additionally, porB-based phylogenetic analysis was performed for the PPNG isolates.

Results The total prevalence of PPNG isolates was 41% (114/276) and 58% (66/114) of these PPNG isolates possessed blaTEM-135. In 2007, 45% (90/199) produced β-lactamase, and of those PPNG, 58% (52/90) possessed blaTEM-135. In 2012, 31% (24/77) were PPNG, and 58% (14/24) of those isolates contained blaTEM-135. There were 162 NG-MAST STs among the 276 isolates, and 89 of those were novel STs. A strong association between specific NG-MAST STs and blaTEM-135 was found, and the porB-based phylogenetic analysis showed a distant evolutionary relationship between isolates in 2007 and isolates in 2012.

Conclusions A high prevalence of PPNG and blaTEM-135 was found in Nanjing, China. blaTEM-135 might be a precursor in the evolution into an extended-spectrum β-lactamase that can degrade ceftriaxone, which stresses the need to continuously monitor PPNG, blaTEM-135, and additional evolving blaTEM gene variants.

A high prevalence of β-lactamase–producing Neisseria gonorrhoeae (41%) and blaTEM-135 (58%) was found in Nanjing, China. blaTEM-135 might be a precursor to an extended-spectrum β-lactamase that can degrade ceftriaxone.

From the *National Center for STD Control, China CDC, and Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College,and Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, China; †National Institute of Infectious Diseases, Tokyo, Japan; and ‡WHO Collaborating Centre for Gonorrhoea and Other STIs, Department of Laboratory Medicine, Microbiology, Örebro University Hospital, Örebro, Sweden

The authors are grateful to Dr Shu-ichi Nakayama (National Institute of Infectious Diseases, Tokyo, Japan) for technical assistance and provision of DNA control sample.

Supported by a grant from the Natural Science Foundation of China (81101294).

All authors declare no conflict of interest.

Correspondence: Yue-Ping Yin, PhD, National Center for STD Control, 12 Jiangwangmiao St, Nanjing 210042, China. E-mail:

Received for publication June 17, 2013, and accepted August 26, 2013.

© Copyright 2013 American Sexually Transmitted Diseases Association