We report new sequence types of 14 penicillinase-producing Neisseria gonorrhoeae, isolated from sexually transmitted disease clinic attendees in Manaus, Brazil. They were characterized by WI/WII/WIII groups, susceptibility testing and Multi-Antigen Sequencing Typing/Mutilocus Sequence Typing protocols. Twelve were classified as WII/III and 2 as WI and were presented resistance to penicillin and tetracycline. New alleles for por and AroE genes and novel sequence types were identified, revealing molecular characteristics not described previously. ST1590 is the common ancestor after eBURST analysis, and these findings represent an important contribution of molecular epidemiology approach in gonococci’s research in Amazonas.
A study of patients in a sexually transmitted disease clinic in Manaus, Amazonas, found novel alleles and clones for the por and AroE genes circulating in the Amazonas region.
Form the *Tropical and Infectious Disease, Universidade do Estado do Amazonas, Fundação Alfredo da Matta-FUAM, Laboratório de Bacteriologia, Clínica Rua Codajás, Manaus, Amazonas, Brazil; †Tropical and Infectious Disease, Fundação Estadual de Hematologia e Hemoterapia do Amazonas, Av. Constantino Nery, Chapada, Manaus, Amazonas, Brazil; ‡Microbiology, Instituto Leônidas e Maria Deane, FIOCRUZ-AM, Rua Teresina, Adrianópolis, Manaus, Amazonas, Brazil; §Clinical Pathology Technician, Fundação de Dermatologia Tropical e Venereologia Alfredo da Matta, Av. Codajás, Manaus, Amazonas, Brazil; and ¶Tropical Disease, Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Av. Pedro Teixeira, n° 25, Bairro D. Pedro, Manaus, Amazonas, Brazil
Institution where the work was performed: Fundação de Dermatologia Tropical e Venereologia Alfredo da Matta, Av. Codajás no. 24, Manaus, Amazonas 69065-130, Brazil.
The authors thank the following: Brazilian Ministry of Health; Maísa Porto dos Santos Biologist; patients and professionals of the Alfredo da Matta’s STI outpatient clinic; Jean Marie Smith (DNASTAR, Inc, Software for live Scientists); and Professor Dr Brian Custer, PhD, Investigator, Blood Systems Research Institute, San Francisco, and Adjunct Associate Professor, Laboratory Medicine University of California San Francisco.
WAF,CMF, FGN contributed equally to this work.
Conflict of interest: None to declare.
Correspondence: William Antunes Ferreira, PhD, Fundação Alfredo da Matta-FUAM, Laboratório de Bacteriologia, Clínica Rua Codajás, no. 25., Manaus, Amazonas 69065-130, Brazil. E-mail: firstname.lastname@example.org.
Received for publication July 17, 2012, and accepted January 7, 2013.