Abstract: A specific real-time polymerase chain reaction followed by melt curve analysis was developed for the detection of the Swedish variant (nvCT) strain of Chlamydia trachomatis (CT). Surveillance was performed on 476 CT-positive clinical specimens obtained from 15 laboratories around the United States using nucleic acid amplification test assays, which would not miss the nvCT. All were negative for nvCT; thus, there is no evidence of the nvCT in the United States.
A real-time polymerase chain reaction for the detection of the new variant Chlamydia trachomatis strain was developed and evaluated for surveillance in the United States. No variants were detected.
From the Division of Infectious Diseases, Departments of *Medicine, †Emergency Medicine, Johns Hopkins University, Baltimore, MD; ‡Infertility Prevention Program, Centers for Disease Control and Prevention, Atlanta, GA; §Department of Epidemiology and Biostatistics, Indiana University, Bloomington, IN; and ¶University of California, San Francisco, CA
We thank all the laboratories that provided specimens for testing.
Funded, in part, by U54EB007958 and HPTN NIAID/NIH U01-AI 068613.
These data were presented, in part, at the Proceedings of the Sixth Meeting of the European Society for Chlamydia Research. P-63, pp. 260, Aarhus, Denmark, July 1–4, 2008.
Correspondence: Charlotte Gaydos, MS, DrPH, Division of Infectious Diseases, Department of Medicine, Johns Hopkins University, 855 N Wolfe St, Rangos Bldg, Rm 530, Baltimore, MD 21205. E-mail: email@example.com.
Received for publication June 6, 2012, and accepted December 18, 2012.