Mucus Digestion Improves the Detection of Chlamydia trachomatis and Neisseria gonorrhoeae on the cobas 4800

Miller, Amanda BBMedSci; Bromhead, Collette PhD; Jones, Mark FRCPA; Tustin, Paul DMLT

Sexually Transmitted Diseases:
doi: 10.1097/OLQ.0b013e318259efe2
Note
Abstract

Abstract: We compared the performance of cobas 4800 PCR for Neisseria gonorrhoeae with culture and found an association between pipetting errors and positive culture, caused by mucopurulent discharge. The problem may be resolved by pretreating samples with Sputasol, thus improving detection of both Chlamydia trachomatis and Neisseria gonorrhoeae.

Author Information

From Aotea Pathology Ltd, Wellington, New Zealand.

Conflicts of interest and sources of funding: none declared.

Correspondence: Collette Bromhead, PhD, Aotea Pathology Ltd, 89 Courtenay Place, Wellington, New Zealand. E-mail: cbromhead@apath.co.nz.

Received for publication February 28, 2012, and accepted April 5, 2012.

© Copyright 2012 American Sexually Transmitted Diseases Association